Anaerobic Gram-positive Cocci Research Articles

Microbiological isolates and associated complications of dacryocystitis and canaliculitis in a prominent tertiary ophthalmic teaching hospital in northern China.

To report the microbiological isolates, aetiology, complications, antibiotic susceptibilities, and clinical remission of dacryocystitis and canaliculitis in a prominent tertiary ophthalmic teaching and referral hospital located in northern China and to offer appropriate recommendations for preventing and formulating drug treatment strategies. This prospective study recruited a total of 477 participants who had been diagnosed with either dacryocystitis or canaliculitis. The cohort comprised 307 patients with chronic dacryocystitis, 111 patients with acute dacryocystitis, and 59 patients with canaliculitis. Purulent discharge from the lacrimal duct was collected using a sterile swab and immediately subjected to microbial culture. Antimicrobial susceptibility testing was conducted following established protocols. All participants were scheduled for follow-up visits within 14 days after receiving antibiotic therapy. The present findings indicated that women exhibited a higher susceptibility to the condition, as evidenced by the occurrence of 367 cases in comparison to 110 cases among men. Among the 477 patients, definitive causes were established in 59 individuals, accounting for 12.4% of the patients. Additionally, ocular complications were reported by 132 patients, representing 27.7% of the total. Monocular involvement was observed in the majority of cases, with 402 out of 477 patients (84.3%) affected, while binocular involvement was present in 75 patients (15.7%). In total, 506 microbiological strains were recovered from 552 eyes, with Staphylococcus epidermidis (16.4%) being the most prevalent microorganism. Other predominant isolates included Corynebacterium macginleyi (9.1%), Staphylococcus aureus (5.1%), Streptococcus pneumoniae (4.9%), Haemophilus (4.4%), Propionibacterium acnes (3.5%), and Eikenella corrodens (3.1%). Among the 12 isolated fungi, Candida parapsilosis accounted for 66.7%. The susceptibility to antimicrobial agents tested in gram-negative bacilli (79.5%) was observed to be higher than that of anaerobic bacteria (76.7%) and gram-positive cocci (55.4%). With pharmacological therapy, the remission rate of acute dacryocystitis (72.7%) was found to be higher than that of canaliculitis (53.3%) and chronic dacryocystitis (42.3%). This study highlights the microbial spectrum of dacryocystitis and canaliculitis, particularly C.macginleyi, E.corrodens and C.parapsilosis, which are also more frequently isolated. Vancomycin and imipenem may be more effective treatment options. Most cases have an unknown aetiology, and essential preventive measures involve postoperative cleansing of the lacrimal passage following eye and nasal surgeries, as well as the proactive management of rhinitis.

Pengaruh Konsentrasi Ekstrak Biji Kakao Terhadap Diameter Zona Bening Pada Bakteri Streptococcus Mutans

Cocoa beans can inhibit the growth of pathogenic bacteria such as Streptococcus Mutans. Utilization of cocoa bean extract as an anti-bacterial inhibitor containing chemical compounds extracted in simplicia aims to increase the content in the form of alkaloids, flavonoids, tannins, saponins and polyphenols. Streptococcus Mutans is a gram-positive facultative anaerobic coccus that is usually found in the human oral cavity and one of the causes of tooth decay or cariogenic bacteria capable of creating an acidic atmosphere in the mouth and can metabolize carbohydrates. This paper describes the effect of the concentration of cocoa bean extract on the diameter of the clear zone of Streptococcus Mutans bacteria. The antibacterial test was carried out using the Mueller Hinton Agar (MHA) method, samples of cocoa bean extraction were prepared by maceration method with varying concentrations of 2.5% to 12.5% ​​at 2.5% intervals. With an interval of three hours, measurements were carried out for 48 hours of observation. The results showed that the greater the concentration of cocoa bean extract, the larger the diameter of the clear zone, with the smallest diameter (8.44 ± 1.61) mm (for a concentration of 2.5%) and the largest diameter (9.70 ± 1.66) mm (for a concentration of 12.5%).

In vitro activity of delafloxacin against anaerobic bacteria compared with other antimicrobials

The aim of this study was to describe the in vitro activity of delafloxacin against 230 anaerobic isolates and compare it with the activity of other antimicrobials used against infections caused by anaerobic microorganisms. Minimal inhibitory concentrations (MICs) were lower for delafloxacin than for all other antibiotics tested with the exception of piperacillin-tazobactam and meropenem against Gram-positive anaerobic cocci. Only two (0.8 %) isolates of Bacteroides spp. showed a MIC ≥4 μg/mL. With some exceptions, the present results show lower MICs for delafloxacin in comparison to the other antibiotics used against anaerobes.

Parvimonas micra, an oral pathobiont associated with colorectal cancer, epigenetically reprograms human colonocytes

ABSTRACT Recently, an intestinal dysbiotic microbiota with enrichment in oral cavity bacteria has been described in colorectal cancer (CRC) patients. Here, we characterize and investigate one of these oral pathobionts, the Gram-positive anaerobic coccus Parvimonas micra. We identified two phylotypes (A and B) exhibiting different phenotypes and adhesion capabilities. We observed a strong association of phylotype A with CRC, with its higher abundance in feces and in tumoral tissue compared with the normal homologous colonic mucosa, which was associated with a distinct methylation status of patients. By developing an in vitro hypoxic co-culture system of human primary colonic cells with anaerobic bacteria, we show that P. micra phylotype A alters the DNA methylation profile promoters of key tumor-suppressor genes, oncogenes, and genes involved in epithelial–mesenchymal transition. In colonic mucosa of CRC patients carrying P. micra phylotype A, we found similar DNA methylation alterations, together with significant enrichment of differentially expressed genes in pathways involved in inflammation, cell adhesion, and regulation of actin cytoskeleton, providing evidence of P. micra’s possible role in the carcinogenic process.

Microbial patterns and culture utility in orbital cellulitis

To determine the prevalence and types of pathogens found in children with orbital cellulitis and to evaluate the utility of nonoperative cultures. This was a retrospective cohort study of children with imaging-confirmed orbital cellulitis over a period of 8 years. Outcomes included prevalence and types of organisms, polymicrobial infection, mixed aerobic-anaerobic infection, effect of age, and culture utility. Of 220 children with orbital cellulitis, 112 (51%) had cultures taken; 69 (31%) had surgical intervention. Culture sources for the 112 children with cultures included blood (57 patients [51%]), sinus (53 [47%]), orbit (42 [38%]), brain (6 [5%]), and skin/conjunctiva/lacrimal sac (6 [5%]). Streptococcus anginosus group strains grew in cultures from 19 children (17%); methicillin-sensitive Staphylococcus aureus (MSSA), in 15 (13%); Streptococcus pyogenes, in 12 (11%); methicillin-resistant Staphylococcus aureus (MRSA), in 6 (5%); anaerobic/facultative gram negative rods, in 8 (7%); anaerobic Gram-positive cocci, other Viridans group streptococci, and Streptococci pneumoniae, in 3 (3%) each; and normal respiratory/skin flora, in 23 (21%). Polymicrobial infection (P = 0.08) and anaerobic organisms (P=0.58) did not differ by age (range, 0.1-16.8 years). In all 220 (100%) children, nonoperative cultures were either not obtained (108 [49%]), not helpful in avoiding surgery (69 [31%]), showed no growth (39 [18%]), or grew an organism that did not change management from empiric therapy (4 [2%]). While many organisms may be cultured from children with orbital cellulitis, Streptococcus and MSSA were the most common in our study cohort. MRSA is uncommon, so initial empiric coverage is not necessary. Rates of polymicrobial and anaerobic infection were similar across ages. Our results indicate that nonoperative cultures are not indicated in the initial medical management of orbital cellulitis; in our cohort, they neither resulted in treatment changes nor helped avoid surgery.

Bacteremia due to Leuconostoc species: A 13-year single-center case series

IntroductionLeuconostoc spp. are facultatively anaerobic Gram-positive cocci involved in cases of hospital-acquired bacteremia, mainly in immunocompromised hosts. The available data is scarce due to its uncommon presentation. MethodsWe describe all the episodes of Leuconostoc spp. bacteremia in a third level hospital in a 13-year period (2008–2021). ResultsFour cases of clinically relevant bacteremia were detected. All cases were categorized as catheter-related. The following risk factors were found: previous glycopeptide therapy (75%), use of parenteral nutrition (100%) and cancer (75%). All isolates showed susceptibility to beta-lactams. Catheter removal was performed and wide spectrum antimicrobials were administered, with clinical response in all cases except one. DiscussionApart from cancer and glycopeptide exposure, disruption of skin barrier and gastrointestinal conditions were identified as risk factors, as it was concordantly underlined in other case series. Susceptibility to beta-lactams is usually maintained. Catheter removal and administration of an active antibacterial therapy seem to be the best approach for Leuconostoc spp. catheter-related bacteremia.

Gram-positive anaerobic cocci guard skin homeostasis by regulating host-defense mechanisms.

In atopic dermatitis (AD), chronic skin inflammation is associated with skin barrier defects and skin microbiome dysbiosis including a lower abundance of Gram-positive anaerobic cocci (GPACs). We here report that, through secreted soluble factors, GPAC rapidly and directly induced epidermal host-defense molecules in cultured human keratinocytes and indirectly via immune-cell activation and cytokines derived thereof. Host-derived antimicrobial peptides known to limit the growth of Staphylococcus aureus-a skin pathogen involved in AD pathology-were strongly upregulated by GPAC-induced signaling through aryl hydrocarbon receptor (AHR)-independent mechanisms, with a concomitant AHR-dependent induction of epidermal differentiation genes and control of pro-inflammatory gene expression in organotypic human epidermis. By these modes of operandi, GPAC may act as an "alarm signal" and protect the skin from pathogenic colonization and infection in the event of skin barrier disruption. Fostering growth or survival of GPAC may be starting point for microbiome-targeted therapeutics in AD.

Group A Streptococcus (Streptococcus pyogenes): the Most Interesting Pathogen in the World

Group A Streptococcus, or Streptococcus pyogenes, is a facultatively anaerobic Gram-positive coccus and one of the most common causes of bacterial infection in humans. The introduction of antibiotics has greatly reduced the morbidity and mortality associated with this organism, but despite uniform susceptibility to treatments of choice, it remains a significant human pathogen. It is particularly problematic in underdeveloped and lower socioeconomic status countries, where hygiene may be suboptimal. This review covers a wide range of topics related to the organism, including diagnosis, treatment, and clinical manifestations. Where possible, the review addresses less conventional and often controversial topics that have not been extensively reviewed elsewhere, such as the activity of trimethoprim sulfamethoxazole against the species, penicillin tolerance, and the use of protein synthesis inhibitors to reduce toxin production and improve outcomes.

Granulicatella spp., a Causative Agent of Infective Endocarditis in Children

Granulicatella spp. are non-motile, non-sporulating, facultatively anaerobic Gram-positive cocci. Throughout the literature, these organisms have been referred to by several names, such as “nutritionally deficient streptococci”, “vitamin-B dependent streptococci” and “pyridoxal-dependent streptococci”, because of their fastidious nutritional requirements, which can often make culture isolation challenging. Known to be a member of the normal microbiota of the human oral cavity and urogenital and intestinal tracts, similar to other streptococci, Granulicatella spp. can cause bacteremia, sepsis and infective endocarditis. Considering the difficulty in growing this organism on culture medium, the fact that it is now included among the bacteria known to be responsible for culture-negative infective endocarditis suggests that its pathogenic role could be highly underestimated. Moreover, being considered such a rare causative agent, it is not a target of standard antibiotic empiric treatment. We present a rare case of G. elegans endocarditis in a young child and review the medical literature on Granulicatella endocarditis in the pediatric population, with the aim of sharing knowledge about this microorganism, which can be challenging for a clinician who is not familiar with it.

Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Chickens.

Rothia nasimurium is a facultative anaerobic Gram-positive coccus belonging to the Rothia genus of the Micrococcaceae family. While Rothia nasimurium is considered an opportunistic pathogen, to date few studies have investigated its pathogenicity and drug resistance. In January 2022, chickens at a poultry farm in China's Xinjiang Uygur Autonomous Region became ill and died. Treatment with commonly used Chinese medicines and antibiotics was ineffective, causing economic losses to the poultry farm. In order to determine the cause of the disease in these poultry farm chickens, the isolation and identification of the pathogens in the livers and other internal organs of the sick and dead chickens were performed. Further, animal pathogenicity tests, antibiotic susceptibility tests, and the detection of antibiotic resistance genes were carried out to analyze the pathogenicity and drug resistance of the identified pathogens. A Gram-positive coccus was isolated from the livers of the diseased chickens. The isolate was resistant to 17 antibiotics, including ciprofloxacin, chloramphenicol, and florfenicol, and was only sensitive to penicillin, amikacin, and tigecycline, to varying degrees. The results of the drug resistance gene testing indicated that the isolated bacterium carried 13 kinds of resistance genes. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, morphological observations, biochemical tests, and 16S rRNA gene sequence analysis were performed on the isolated bacterium, and it was determined that the isolated bacterial strain was Rothia nasimurium. The animal pathogenicity tests showed that the isolate caused feather loss and death in chicks; the clinical symptoms and necropsy lesions of the test chicks were consistent with those observed in the farmed chickens. A review of the literature revealed that, to date, there are no reports of infection with Rothia nasimurium in chickens. Thus, in this study, Rothia nasimurium was isolated from chickens for the first time and an investigation of the biological characteristics of the bacterium was carried out in order to provide a reference for the clinical treatment, prevention, and control of Rothia nasimurium infection.

Culprit or Innocent Bystander? A Case of Hematemesis.

An 11-year-old previously healthy female presented with 1 year history of generalized abdominal pain, early satiety and intermittent hematemesis without history of melena. She underwent esophagogastroduodenoscopy (EGD) as a part of her workup. EGD was notable for diffuse gastric erythema and retained food in stomach despite being nil per os for solid food for 14 hours (Fig. 1). Histopathologic examination showed chronic gastritis in the gastric body and focal organisms identified morphologically as Sarcina ventriculi (Fig. 2).FIGURE 1.: Gastric mucosal erythema and retained food in stomach.FIGURE 2.: Photomicrograph showing S. ventriculi organisms in the stomach mucosa with characteristic morphology: Aggregates of basophilic stained, cuboid organisms in tetrad packet arrangement with flattening of the cell walls in areas of contact with adjacent cells. Hematoxylin and eosin stain, original magnification ×40.S. ventriculi is a Gram-positive anaerobic coccus with a birefringent cellulose coating, averaging from 1.8 to 3 μm in size, that is found in water and soil and thrives in low pH environment. In the stomach, it appears as clusters of tetrads. Its metabolism is strictly carbohydrate dependent. It is unclear if S ventriculi has a pathogenic role, but it has been associated with gastroparesis, gastric ulcers, mucosal inflammation (1), emphysematous gastritis (2), gastric perforation (3), and esophagitis (4). Local accumulation of acetaldehyde and ethanol from carbohydrate fermentation by S. ventriculi may cause gastric mucosal injury. The most common endoscopic feature (5), which was found in this patient, is the presence of food retention due to delayed gastric emptying, often accompanied by an inflamed gastric mucosa. There are neither consensus on which patients need treatment nor guidelines regarding antibiotic selection or duration of treatment. In this case, the child received empiric treatment with metronidazole and amoxicillin/clavulanic acid for 1 week along with a proton-pump inhibitor for 2 weeks, with resolution of hematemesis and improvement of her abdominal pain. ACKNOWLEDGMENTS Informed consent was provided by patient’s parents for this report to be published

Epidemiology and antibiotic susceptibility profiles of obligate anaerobes in a hospital of central Italy during a one-year (2019) survey

ObjectivesIn this study, we report on the epidemiology and susceptibility profiles of anaerobic pathogens consecutively isolated from various clinical samples at an Italian hospital during a one-year survey. MethodsThe collection included all non-duplicated consecutively collected anaerobic clinical isolates during 2019 in San Luca Hospital (Lucca, Italy). Antimicrobial susceptibility testing was performed using MICRONAUT-S Anaerobes MIC lyophilized plates (MERLIN Diagnostika, Germany) and interpreted using EUCAST criteria (11.0). ResultsA total of 169 Gram-negative and 213 Gram-positive were collected. The most frequent anaerobes were Bacteroides spp. (120, 31.4%) followed by Finegoldia magna (62, 16.2%). External ulcers were the most common source of isolates (39%), followed by blood (25.7%). In 230 patients (65%) polymicrobial aerobic/anaerobic isolates were cultured from the same external ulcer specimen. High resistance rates to clindamycin were overall detected, with the highest values among the genera Parabacteroides, Bacteroides, Prevotella, Gram-positive anaerobic cocci and Clostridium. High resistance rates were also observed to metronidazole among both Gram-positive and Gram-negative species, ranging between 10.8-50% and 13.8–46.2%, respectively. ConclusionsOur findings revealed that anaerobes susceptibility patterns have become less predictable due to an increase of resistance and suggest that periodic resistance surveillance should also be carried out for anaerobes in order to guide empirical therapy.

WHEN FLU LEADS THE WAY: A CASE OF PARVIMONAS MICRA POLYMICROBIAL EMPYEMA IN A PATIENT WITH INFLUENZA A INFECTION

WHEN FLU LEADS THE WAY: A CASE OF PARVIMONAS MICRA POLYMICROBIAL EMPYEMA IN A PATIENT WITH INFLUENZA A INFECTION

Incidence and Clinical Characteristics of Anaerobic Bacteremia at a University Hospital in Hungary: A 5-Year Retrospective Observational Study.

Strict anaerobes have been reported to account for 0.5–13% of episodes of bacteremia in the adult population, with a growing awareness among clinicians regarding anaerobic bacteremia, especially in patients with specific predisposing factors. The aim of our present study was to assess the incidence and clinical characteristics of anaerobic bacteremia during a 5-year period (2016–2020) at a tertiary care teaching hospital, and to compare our findings with other studies in Hungary. Overall, n = 160 strict anaerobes were detected, out of which, 44.4% (n = 71; 0.1% of positive blood cultures, 0.1/1000 hospitalizations, 3.3/100,000 patient days) were clinically significant, while Cutibacterium spp. accounted for 55.6% (n = 89) of isolates. Among relevant pathogens, the Bacteroides/Parabacteroides spp. group (32.4%; n = 23), Clostridium spp. (22.5%; n = 16) and Gram-positive anaerobic cocci (15.5%; n = 11) were the most common. The mean age of patients was 67.1 ± 14.1 years, with a male majority (59.2%; n = 42). A total of 38.0% of patients were affected by a malignancy or immunosuppression, while an abscess was identified in 15.5% of cases. A total of 74.7% (n = 53) of patients received antibiotics prior to blood culture sampling; in instances where antimicrobials were reported, anaerobic coverage of the drugs was appropriate in 52.1% (n = 37) of cases. The 30-day crude mortality rate was 39.4% (n = 28); age ≥ 75 years was a significant predictor of 30-day mortality (OR: 5.0; CI: 1.8–14.4; p = 0.003), while malignancy and immunosuppression, lack of anti-anaerobic coverage or female sex did not show a significant relationship with the mortality of these patients. Early recognition of the role played by anaerobes in sepsis and timely initiation of adequate, effective antimicrobial treatment have proven efficient in reducing the mortality of patients affected by anaerobic bacteremia.

A Multi-Criteria Approach for Comparison of Ginger Extract and Conventional Irrigants in Root Canal Treatment.

BackgroundConsidering the importance of irrigation in dental root canal treatment, there is an urgent need to find a risk-free bioactive and antibacterial endodontic solution. Enterococcus faecalis, an anaerobic gram-positive coccus, has been identified as the main reason for endodontic infections. Several studies have been conducted on E. faecalis and periapical infection. Nowadays, plants used in traditional medicine play a role that is widely appreciated by researchers. One of these herbs is ginger which shows an acceptable antimicrobial effect on E. faecalis. Due to the highly crucial role that irrigation plays in the success of endodontic treatment, a comprehensive survey based on several criteria, namely, scientific, technical, and empirical, is required to address the goal of determining the best endodontic solution.MethodologyThe most important criteria are antibacterial activity, risks and hazards, cost, and availability. In this study, the analytical network process (ANP), which is a multi-criteria decision-making method, was applied to determine the best endodontic irrigant.ResultsSeveral alternatives were investigated using the ANP. In this study, 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine were at the top of the list. According to the sensitivity analysis, 10% ethanolic ginger extract showed comparable results to 2.5% NaOCl.ConclusionsTo carefully prioritize endodontic irrigants a wide range of standards and criteria should be considered. Considering the low risk, great wettability, and active compounds of ginger extract, it can be a promising viable risk-free solution for root canal treatments.

Multi-year comparison of VITEK® MS and 16S rRNA gene sequencing performance for the identification of rarely encountered anaerobes causing invasive human infections in a large Canadian region: can our laboratory abandon 16S rRNA gene sequencing?

BackgroundOur large regional laboratory routinely provides a definitive identification (ID) for 800-1,200 anaerobic bacteria per annum that cause invasive human infections. An increasing number of isolates (i.e., 10 to 13%) recovered from clinical specimens from these cases were more unusual or rarely isolated genera and/or species (i.e., ≤5 individual cases/annum). MethodsVITEK® MS (MALDI-TOF MS) is done initially on all anaerobic bacteria, but rare isolates undergo in-house PCR/sequencing when proteomics provides a wrong ID or no results despite repeat testing. A clinical microbiologist in consultation with the Infectious Diseases service approves molecular analyses. This multi-year comparison (2014-19) of the performance of MALDI-TOF MS and 16S rRNA gene sequencing using the IDNS® SmartGene bacterial dataset shows both method's abilities to provide a genus-level and/or species-level ID for rare isolates. Results489 rare anaerobes were recovered from a variety of clinical specimens: 57% blood cultures, 19% other sterile fluids, 14% sterile tissues, 8% deep wounds/abscesses, and 2% prosthetic implants. 16S rRNA gene sequencing gave an accurate genus-vs. species level ID for 487/489 (99.6%) and 401/489 (82.0%) of isolates respectively. Accurate genus-vs species-level ID were obtained by MALDI-TOF MS for 269/489 (53.4%) and 187/489 (37.3%) of isolates respectively. MALDI-TOF MS gave wrong or no results for 35.1% of Gram-negative anaerobic cocci (GNAC), 62% of Gram-negative anaerobic bacilli (GNAB), 30.8% of Gram-positive anaerobic cocci (GPAC) and 46.3% of Gram-positive anaerobic bacilli (GPAB). Neither method gave an ID for one GNAB and one GPAC isolate. MALDI-TOF MS genus-level ID of GNAC and genus/species-level ID of GPAB improved during the study but its performance remained stable for genus- or species-level ID of other organism groups. ConclusionsMALDI-TOF MS provides accurate ID for most common anaerobes, but molecular analyses need to be available for rare isolates. Large complex laboratories should have a workflow for sending rare isolates for 16S rRNA gene sequencing in invasive cases where a definitive ID is clinically required.

Antibiotic Resistance and Mechanisms of Pathogenic Bacteria in Tubo-Ovarian Abscess.

A tubo-ovarian abscess (TOA) is a common type of inflammatory lump in clinical practice. TOA is an important, life-threatening disease, and it has become more common in recent years, posing a major health risk to women. Broad-spectrum antimicrobial agents are necessary to cover the most likely pathogens because the pathogens that cause TOA are polymicrobial. However, the response rate of antibiotic treatment is about 70%, whereas one-third of patients have poor clinical consequences and they require drainage or surgery. Rising antimicrobial resistance serves as a significant reason for the unsatisfactory medical outcomes. It is important to study the antibiotic resistance mechanism of TOA pathogens in solving the problems of multi-drug resistant strains. This paper focuses on the most common pathogenic bacteria isolated from TOA specimens and discusses the emerging trends and epidemiology of resistant Escherichia coli, Bacteroides fragilis, and gram-positive anaerobic cocci. Besides that, new methods that aim to solve the antibiotic resistance of related pathogens are discussed, such as CRISPR, nanoparticles, bacteriophages, antimicrobial peptides, and pathogen-specific monoclonal antibodies. Through this review, we hope to reveal the current situation of antibiotic resistance of common TOA pathogens, relevant mechanisms, and possible antibacterial strategies, providing references for the clinical treatment of drug-resistant pathogens.

Profile of Multidrug Resistance and Methicillin-Resistant Staphylococcus aureus (MRSA) on dairy cows and risk factors from farmer

Abstract. Khairullah AR, Sudjarwo SA, Effendi MH, Ramandininto SC, Gelolodo MA, Widodo A, Riwu KHP, Kurniawati DA, Rehman S. 2022. Profile of Multidrug Resistance and Methicillin-Resistant Staphylococcus aureus (MRSA) on dairy cows and risk factors from farmer. Biodiversitas 23: 2853-2858. Staphylococcus aureus is a facultative anaerobic Gram-positive coccus that causes mastitis and is one of the most widespread pathogenic bacteria in dairy farming. These bacteria can quickly develop into antibiotic-resistant strains due to inappropriate antibiotic administration. Staphylococcus aureus bacteria that have Multidrug-Resistant (MDR) properties can be known as Methicillin-Resistant S. aureus (MRSA) if these bacteria are resistant to beta-lactam antibiotics. The high number of MDR and MRSA contamination in dairy farms as a result of excessive administration of antibiotics in the treatment of dairy cows and the spread of these bacteria cannot be separated from the sanitation management at the time of milking, it can be from milk that is milked from the udder and it can also be from the hands of farmers during the milking process. This study tests antibiotic resistance and detects Methicillin-Resistant S. aureus (MRSA) strains sourced from milk and hand swabs of farmers in Probolinggo, East Java, Indonesia. A total of 109 dairy cow’s milk samples and 41 samples of farmers’ hand swabs were taken from each farm. Samples were cultured and purified using Mannitol Salt Agar (MSA). Staphylococcus aureus resistance profile was performed by disk diffusion test using antibiotic disks such as Oxacillin, Cefoxitin, Tetracycline, Erythromycin, and Gentamicin. Staphylococcus aureus isolates that were resistant to Oxacillin and Cefoxitin antibiotics were then tested for Oxacillin Resistance Screening Agar Base (ORSAB) as a confirmation test for Methicillin-Resistant S. aureus (MRSA). The results of the isolation and identification found 80 (53.33%) S. aureus isolates, then the results of the resistance test found 8 (10%) S. aureus isolates that were Multidrug-Resistant (MDR), and in the Oxacillin Resistance Screening Agar Base (ORSAB) test there were 3 isolates of S. aureus MDR were ORSAB positive. It can be found that there are several S. aureus isolates that are Multidrug-Resistant (MDR) and Methicillin-Resistant S. aureus (MRSA) in dairy farms. The S. aureus that is MDR and MRSA can be spread to the community and will endanger public health. Thus, prevention and control measures are needed to suppress the spread of S. aureus infection on a dairy farm in Probolinggo, East Java, Indonesia.

BRAIN ABSCESS CAUSED BY PARVIMONAS MICRA: A RARE CASE REPORT

Parvimonas micra are anaerobic gram-positive cocci, usually found in the human oral mucosa. We present a case of cerebral abscess caused by Parvimonas micra, an infrequent agent for this pathology, in a 03year old male child known case of congenital heart disease (Hypoplastic right heart with critical pulmonary stenosis operated) and Left dysplastic Kidney. Initially he was conscious, irritable, febrile (102.F), had cyanosis, clubbing, cervical lymphadenopathy and raised JVP (Jugular Venous Pressure). He was evaluated with MRI Brain which showed left temporo-parietal abscess. He underwent emergency left temporo-parietal craniotomy and excision of abscess cavity along with Ommaya chamber placement. Approximately 100ml of abscess was drained sent for culture sensitivity. Postoperatively, he was managed with Intravenous uids, anticonvulsants, cerebral decongestants and antibiotics (Ceftriaxone and Vancomycin).

Metagenomics of Cariogenic Microbiota in Primary Teeth with Deep Dentinal Caries: An Illumina-Based Sequencing Study

Discrepancy in the dynamic metabolic progression of the dental biofilm leads to dental caries, which is a polymicrobial infection. The homeostasis of the biofilm is unaffected and is not apparent unless there is an imbalance in the mineralization pattern of the supragingival biofilm. Literature provides numerous facts on microorganisms related to dental caries. Pathologic colonizers in the early stages of tooth decay involve organisms like Streptococcus oralis, subsequent adherence of Streptococcus sobrinus, and Streptococcus mutans[1]. Reports suggest the association of highly complex, variable, and uncultivable microbial population with dental caries, even though prime organisms are commonly known to be allied with disease succession. Association of mutans group streptococci and lactobacilli with the initiation of caries and early caries development have been well documented [2]. Studies have shown transition of early caries causing gram-positive primary facultative bacteria to anaerobic gram-positive rods and cocci and gram-negative rods as the lesion progresses to deep carious lesion[3]. Mapping of bacterial taxa revealed up to 14 phyla along with 101 genera. This comprised around 706 diverse OTUs (Operational Taxonomic Units). Caries progression at various stages and bacterial profile of different species associated with several disease have been observed. Some of the observed species found abundantly in whitespot lesion and dentin lesion of both dentitions were S. Parasanguinis and S. salivarius[4]. Molecular methods for bacterial identification and enumeration are performed routinely to precisely study bacterial species associated with dental caries, including those that are not presently cultivable.