Lung cancer is presently the deadliest cancer worldwide, which causes large quantities of deaths, estimated more than 1.8 million, globally every year. Meanwhile, the diagnosis and prognosis for the early-stage lung cancer patients is always absent even until recent years, which has led to the result that the patients are mostly diagnosed with lung cancer in advanced stage. The present study searches for biomarkers related to early stage diagnosis so as to help deal with this factual problem. After analyzing plenty of samples on Kpalan-Meier plotter websites, this research discovers that ALK, GALNT14, TIMP1 are negatively related with OS of lung cancer patients. Further stratified analysis reveals that three proteins can indicate poor prognosis in the patients who got adenocarcinoma and were in early stage or grade. In addition, the present study also finds that the three proteins can respectively work as prognosis factor among three different groups of patients divided by gender, smoking history, and whether receiving chemotherapy or not. Conclusively, this research have discovered three biomarkers which can be applied in diagnosis, prognosis and treatment in the patients with early stage or grade of lung cancer.