Gradient Mode Research Articles

Performance prediction applying different reduced turbulence models to the SMART tokamak

Abstract The SMall Aspect Ratio Tokamak (SMART) is currently being
commissioned at the University of Seville and will be able to compare the
performance of positive and negative triangularity plasmas at low aspect ratio.
Predictive simulations have been performed for different machine scenarios and
heating schemes using the TRANSP code. The objectives of these simulations
are to predict the parameters expected in positive triangularity plasmas, to
guide diagnostic development, and to validate transport models. Several reduced
turbulence models have been used to predict electron and ion temperatures for the
operational phase 2. All models provide similar results from approximately midradius
to the separatrix but important discrepancies are found in the core region.
These positive triangularity results are compared with experiments from a similar
size machine like GLOBUS-M2. The multi-mode model (MMM) shows the best
agreement. Simulations with different boundary conditions have been performed
and no strong differences have been observed between them. The impact of neutral
beam injection (NBI) on the predicted profiles has also been addressed. Rotation
reduces turbulence levels so higher temperatures are achieved when included in
the simulations. Studying the different contributions to the thermal diffusivities,
it is observed that electron temperature gradient (ETG) turbulence dominates
at the plasma core while mictro-tearing modes (MTM) dominate at the edge
in the electron channel. In the ion channel, the neoclassical contribution is
dominant at the core and at the very edge while the Weiland component, which
includes ion temperature gradient mode (ITG), trapped electron mode (TEM),
kinetic ballooning mode (KBM), peeling mode (PM) and collisionless and collision
dominated magnetohydrodynamic (MHD) modes governs the mid-radius region.
For phase 3, two plasmas with different electron densities have been studied. The
case with lower density matches well a specific discharge of GLOBUS-M2. The
higher density plasma shows high performance with βN ≈ 3.8.

RP-HPLC Method for Simultaneous Quantification of Withanolides in <i>Withania somnifera</i>

Background: Withanolides are the primary bioactive components of Withania somnifera (L.) Dunal. Among them, withanolide A, and 12-deoxy-withastramonolide are the herb’s most physiologically active ingredients. The steroidal lactones are isomers, which makes them difficult to separate. Aim: In the current research, a simple, precise, accurate, and decent RP-HPLC method has been developed, optimised and validated for simultaneous quantification and separation. Methods: Resolution was conducted on Gemini, Phenominex C18 (250mm X 4.6mm, 5 μ) column at 40oC utilising binary mobile phases of acetonitrile and ammonium acetate (10 mM) in gradient mode with 1.0 mL/min flow rate and observed at 230 nm. The total run time was twenty-five minutes. The procedure complied with ICH guidelines, covering the limit of detection, specificity, linearity, quantification, precision, accuracy, and robustness. Results: The standard curves of both relevant analytes displayed a linear pattern with regression values > 0.999 in the 1–10 μg/mL range. For both herbal markers, 1 μg/mL was the lowest limit of quantification. The method’s accuracy was 100.38-100.93 % and 99.13-100.75 % for with withanolide A, and 12-deoxy-withastramonolide respectively. The precision of within and between days was found in the tolerable limit of >2% for both herbal markers. Conclusion: The suggested method worked well for simultaneously analyzing the extracted samples and identifying withanolides.

Advanced surrogate model for electron-scale turbulence in tokamak pedestals

We derive an advanced surrogate model for predicting turbulent transport at the edge of tokamaks driven by electron temperature gradient (ETG) modes. Our derivation is based on a recently developed sensitivity-driven sparse grid interpolation approach for uncertainty quantification and sensitivity analysis at scale, which informs the set of parameters that define the surrogate model as a scaling law. Our model reveals that ETG-driven electron heat flux is influenced by the safety factor $q$ , electron beta $\beta _e$ and normalized electron Debye length $\lambda _D$ , in addition to well-established parameters such as the electron temperature and density gradients. To assess the trustworthiness of our model's predictions beyond training, we compute prediction intervals using bootstrapping. The surrogate model's predictive power is tested across a wide range of parameter values, including within-distribution testing parameters (to verify our model) as well as out-of-bounds and out-of-distribution testing (to validate the proposed model). Overall, validation efforts show that our model competes well with, or can even outperform, existing scaling laws in predicting ETG-driven transport.

A sustainable RP-HPLC method for concurrent estimation of capecitabine and celecoxib in liposomal formulation: Greenness and whiteness appraisal.

Liposomes have been reported for combination therapy due to their ability to carry both hydrophilic and lipophilic drugs together. The current investigation aims to develop a novel, eco-friendly, and sustainable reverse-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous quantification of capecitabine and celecoxib co-encapsulated in liposomes. The method reported herein uses a C18 column (4.6 × 250 mm2, 5 μm) and a mobile phase consisting of water, and acetonitrile/methanol in a ratio of 60:40, containing 0.1% formic acid in both the phases. The flow rate is maintained at 1 mL/min, with an injection volume of 10 μL in the gradient mode. Detection is set at λmax = 240 nm for capecitabine and 252 nm for celecoxib. The developed liposomes are mono-disperse with a surface potential of -6.93 mV. The average size of the liposomes is 142 nm. The percentage entrapment efficiency for capecitabine is 52.39 ± 0.94%, and for celecoxib, it is 77.13 ± 0.74%. The Analytical Greenness Metric of 0.61 and Analytical Eco-Scale Score of 75 signify the greenness of the developed method. Also, the Red-Green-Blue model shows excellent whiteness, with a score of 83.2. Thus, the developed method offers a reliable, accurate, precise, buffer-free, and environment-friendly RP-HPLC approach for the simultaneous analysis of capecitabine and celecoxib co-encapsulated in liposomes.

In situ Single-Pass Perfused Rabbit Intestinal Study sample Analysis of Ractopamine by UPLC

In recent years, the prediction of oral drug absorption in humans has been a challenge for researchers and many techniques for permeability studies have been developed for several purposes, including biowaiver processes. The Single-Pass Intestinal Perfusion (SPIP) method performed in rats can provide permeability results closest to in vivo condition. he use of SPIP in rats and calculations for absorption prediction in humans may indicate the transport mechanisms and/or pre-systemic metabolism involved on permeation processes of drugs, since this model is the closest to in vivo conditions. Ractopamine hydrochloride is a commercial beta-adrenergic agonist commonly used as a dietary supplement in cattle production for improved feed efficiency and growth promotion. Currently, regulatory target tissues (as approved in the New Animal Drug Application with Food and Drug Administration) for ractopamine residue testing are muscle and liver. The aim of the study is to develop and validate an UPLC assay method to determine whether detectable and quantifiable levels of perfused ractopamine in small intestine of cattle or not. The separation is carried on Acquity UPLC BEH C18 (150mm x 2.1mm, 1.7 µm) reversed phase column using acetonitrile and 0.01M ammonium formate as the mobile phase in gradient mode at flow rate of 0.5mL/min. The precision, accuracy, extraction recovery, matrix effect, and stability meet the requirements of the guiding principles. In order to verify the sensitivity and selectivity of the method in a real-time situation, the developed method is successfully applied to a perfusion study in small intestine of rabbit and the samples were analyzed by Empower software.

Ion temperature gradient modes modulational stability with kappa-distribution

We investigated the modulational stability and instability of the ion temperature gradient (ITG) mode in electron-ion plasma. Ions are dynamic species, whereas electrons follow a Kappa distribution. We used the reduction perturbation approach to determine the linear dispersion relation for the fluid under consideration. The nonlinear Schrodinger equation describes nonlinear features such as nonlinear modulational stability or instability in the ITG mode. The product of dissipation and nonlinear coefficients, known as LM, contains both modulational stability and instability in the ion temperature gradient mode. Theoretical results are expanded numerically, showing the influence of various plasma parameters on modulational stability and instability, particularly the superthermality coefficient κ e . The current observations may be extended to space and laboratory plasma for modification.

First results of turbulence investigation on Globus-M2 using radial correlation Doppler reflectometry

The first results of investigation of the turbulence structure using Doppler backscattering (DBS) on the Globus-M2 tokamak are presented. A one-channel DBS system with a variable probing frequency within the 18–26 GHz range was installed to investigate the edge plasma at normalized minor radii 0.9–1.1. Radial correlation Doppler reflectometry was used to study the changes in turbulence eddies after the LH transition. Correlation analysis was applied to the phase derivative of complex in-phase and quadrature (IQ) signals of the DBS diagnostic as it contains information about the poloidal plasma rotation velocity. In L-mode, the radial correlation length L r is estimated to be 3 cm and after transition to H-mode reduces to approximately 2 cm. Gyrokinetic modelling in a linear local approximation using code GENE indicates that the instability with positive growth rate at the normalized minor radius 0.75 in L-mode and H-mode on Globus-M2 was the ion temperature gradient (ITG) mode.

Hybrid organic monolithic column containing MIL-68(Al) for the separation of small molecules by capillary HPLC

A hybrid organic monolithic column made of poly(lauryl methacrylate-co-1,6-hexanediol dimethacrylate) and the metal-organic framework MIL-68(Al) was prepared for the first time. The column was used in capillary liquid chromatography, both in isocratic and gradient elution modes. Separation performance towards small molecules of different chemical nature (polycyclic aromatic hydrocarbons, alkylbenzenes, phenols, etc.) was studied. Monte Carlo simulations were made to both select the proper precursors to obtain empty metal-organic framework micropores in the monolithic polymer and also, to analyze the potential free access of the studied analytes into the micropores (necessary to improve mass transfer and column efficiency). The hereby synthesized metal-organic framework microcrystals allowed obtaining homogeneous hybrid monolithic columns. Adding of MIL-68(Al) (1030 m2 g-1 BET specific surface area) increased the surface area from 3.9 m2 g-1 for the parent monolith to 18.2 m2 g-1 for the hybrid column containing 8 mg mL-1 of the microcrystals. Chromatographic performance of this new column was evaluated by studying retention factors, resolution, and plate counts at room temperature. Different compounds, not completely resolved in the parent monolith, were partially or completely separated after metal-organic framework addition. Using the monolithic column with only 2 mg mL-1 of MIL-68(Al), five alkylbenzenes were completely separated with very symmetrical peak shapes, resolution factors up to 3.60 and plate counts of 4300 plates m-1 for n-hexylbenzene. This value is higher than those obtained by other authors who used organic monolithic columns with embedded metal-organic frameworks to perform separations at room temperature. Additionally, nine polycyclic aromatic hydrocarbons were partially or completely resolved in gradient elution mode. The hybrid monolithic columns exhibited very good intra-day (%RSD=1.9), inter-day (%RSD=2.6), and column-to-column (%RSD=4.3) reproducibility values. Easy and fast column preparation, and versatility to efficiently separate several compounds of different chemical nature in isocratic and gradient mode, makes this new hybrid column a very good option for the analysis of small molecules in capillary (or nano) HPLC.

Magnetic shaping effects on turbulence in ADITYA-U tokamak

The work reported in this paper addresses two aspects. In the first part, numerical simulations are conducted to examine the impact of magnetic equilibrium shaping (elongation and triangularity), on both conventional Ion Temperature Gradient (ITG) modes and Short Wavelength ITG modes. This analysis is performed considering the experimental profiles and parameters of the ADITYA-U tokamak, employing the nonlinear global gyrokinetic Particle-in-Cell code ORB5. The linear and nonlinear collisionless electrostatic simulation of these modes are carried out with kinetic ions and adiabatic electrons. From the linear results, it has been observed that the magnetic equilibrium shaping slighty reduced the growth rates and widened the spectrum, and the maxima of growth rate curve is shifted to higher toroidal wave number. We find that the heat flux is reduced by a significant ≃35% for the true circular magnetohydrodynamic magnetic equilibrium as compared to ad hoc concentric circular equilibrium reported in Singh et al (2023 Nucl. Fusion 63 086029). A further ≃10% reduction in the heat flux is seen with magnetic equilibrium shaping. In the second part, linear collisionless electrostatic simulation studies of ITG coupled with fully kinetic electrons (both trapped and passing electrons are treated kinetically) using a drift-kinetic ordering is performed. It can be seen from the linear results that, in presence of kinetic electrons, the growth rate and real frequency of the ITG mode are increased significantly for ADITYA-U parameters and a mode propagating in the electron diamagnetic direction is identified at high toroidal wavenumbers.

Millimeter-wave high-wavenumber scattering diagnostic developments on EAST and NSTX-U.

A pioneering 4-channel, high-k poloidal, millimeter-wave collective scattering system has been successfully developed for the Experimental Advanced Superconducting Tokamak (EAST). Engineered to explore high-k electron density fluctuations, this innovative system deploys a 270GHz mm-wave probe beam launched from Port K and directed toward Port P (both ports lie on the midplane and are 110° part), where large aperture optics capture radiation across four simultaneous scattering angles. Tailored to measure density fluctuations with a poloidal wavenumber of up to 20cm-1, this high-k scattering system underwent rigorous laboratory testing in 2023, and the installation is currently being carried out on EAST. Its primary purpose lies in scrutinizing ion and electron-scale instabilities, such as the electron temperature gradient (ETG) mode, by furnishing measurements of the kθ (poloidal wavenumber) spectrum. This advancement significantly bolsters the capacity to probe high-k electron density fluctuations within the framework of EAST. Beam tracing and data interpretation modules developed for both EAST and NSTX-U high-k scattering diagnostics are described.

New millimeter-wave diagnostics to locally probe internal density and magnetic field fluctuations in National Spherical Torus Experiment-Upgrade (invited).

A set of new millimeter-wave diagnostics will deliver unique measurement capabilities for National Spherical Torus Experiment-Upgrade to address a variety of plasma instabilities believed to be important in determining thermal and particle transport, such as micro-tearing, global Alfvén eigenmodes, kinetic ballooning, trapped electron, and electron temperature gradient modes. These diagnostics include a new integrated intermediate-k Doppler backscattering (DBS) and cross-polarization scattering (CPS) system (four channels, 82.5-87GHz) to measure density and magnetic fluctuations, respectively. The system can access reasonably large normalized wavenumbers kθρs ranging from ≤0.5 to 15 (where ion sound gyroradius ρs = 1cm and kθ is the binormal density turbulence wavenumber). The system addresses the challenges for making useful DBS/CPS measurements with a remote control of launch polarization (X- or O-mode), probed wavenumber, polarization match of the launch beam with the edge magnetic field pitch angle, and beam steering of the launched beam for wave-vector alignment. In addition, a low-k DBS system consisting of eight fixed frequencies (34-52GHz) and four tunable frequencies (55-75GHz) for low-k density turbulence and fast ion physics will be located at a nearby port location. The combined systems cover the near LCFS and pedestal regions (34-52GHz), the pedestal or mid-radius (50-75GHz), and core plasmas (82.5-87GHz).

Verification and validation of electrostatic turbulence simulation for numerical Lie transform code

Abstract This paper reports the verification and validation of a new gyrokinetic (GK) code, numerical Lie transform (NLT), for electrostatic turbulence simulation in tokamak plasmas. Based on the standard cyclone base case (CBC), NLT has been verified against two typical GK codes, GK toroidal code (GTC) and GK numerical experiment of tokamak, by simulating the ion temperature gradient mode and trapped electron mode. The linear dispersion relation and mode structure agree well among all these codes. In nonlinear simulation, the time evolution of ion heat diffusivity obtained from NLT is consistent with the GTC results. Furthermore, utilizing experimental equilibria with obvious turbulence features from HL-2A shot #22388, the validation of NLT for real tokamak simulation has been carried out. The profiles of ion turbulent thermal diffusivity calculated by NLT match well with experimental results.

A Quality by Design (QbD) driven gradient high performance liquid chromatography method development for the simultaneous estimation of dasatinib and nilotinib in lipid nanocarriers

Tyrosine kinase inhibitors (TKIs) are effective as a targeted treatment for chronic myeloid leukemia (CML), which can selectively suppress BCR-ABL1 kinase activity. CML therapy with TKIs combination has been supported by in-vitro, in-vivo, and patient-based data where the nilotinib-dasatinib co-administration has exerted superior anticancer efficacy with greater cellular uptake, less resistance to chemotherapy, and no additive adverse events encountered. Therefore, it is essential to develop a suitable analytical method for the simultaneous estimation of these drugs in the developed novel lipid nanocarriers like liposomes. Design of Experiment (DoE) has been implemented as a tool of QbD to systematically investigate the relation between the HPLC method attributes and analytical responses, i.e., chromatographic detection, quantification, and peak properties for dasatinib and nilotinib. An Ishikawa diagram is constructed to delineate possible influencing variables to the analytical performances. Afterward, 4 factors 2 level full factorial design (FFD) was employed to model and identify the main effects and interaction effects between the factors selected after the initial risk assessment. The suggested design space for optimized chromatographic conditions by QbD analysis is linear within the selected range of drug concentrations, accurate and precise, sensitive, and robust according to the ICH guidelines. The optimal method is comprised of a 1 mL/min flow rate of mobile phase (ACN and 20 mM KH2PO4 of pH 7.00) in gradient mode at 25 °C column temperature for 20 μL sample injection volume and detection wavelength fixed at 297 nm. Most importantly, this novel HPLC method is simple and selective enough to evaluate dasatinib and nilotinib content in the lipid nanocarriers.

Spherical bispectrum expansion and quadratic estimators

We describe a general expansion of spherical (full-sky) bispectra into a set of orthogonal modes. For squeezed shapes, the basis separates physically-distinct signals and is dominated by the lowest moments. In terms of reduced bispectra, we identify a set of discrete polynomials that are pairwise orthogonal with respect to the relevant Wigner 3j symbol, and reduce to Chebyshev polynomials in the flat-sky (high-momentum) limit for both parity-even and parity-odd cases. For squeezed shapes, the flat-sky limit is equivalent to previous moment expansions used for CMB bispectra and quadratic estimators, but in general reduces to a distinct expansion in the angular dependence of triangles at fixed total side length (momentum).We use the full-sky expansion to construct a tower of orthogonal CMB lensing quadratic estimators and construct estimators that are immune to foregrounds like point sources or noise inhomogeneities. In parity-even combinations (such as the lensing gradient mode from TT, or the lensing curl mode from EB) the leading two modes can be identified with information from the magnification and shear respectively, whereas the parity-odd combinations are shear-only. Although not directly separable, we show that these estimators can nonetheless be evaluated numerically sufficiently easily.

Estimation of Glyoxylic Acid in Emtricitabine by a New Validated RP-HPLC Method

To establish a simple, sensitive, as well as specified method for the separation and quantitative determination of glyoxylic acid in emtricitabine by using reversed-phase high-performance liquid chromatography (RP-HPLC). A superior separation of glyoxylic acid in emtricitabine was done by using a C8 column and a mobile system of (0.1% OPA and ACN: H2O) with 1-mL/min flow rate in gradient mode and a wavelength of 210 nm. The glyoxylic acid was eluted at 5.37 minutes with good efficiency and system suitability. The method has shown good linearity ranges from a limit of detection (LoQ) to 150% level of glyoxylic acid standard solution (0.1% or 0.001 mg/mL). ICH principles validated the suggested approach, and every parameter met the requirements for the International Council of Harmonization (ICH) Q2 recognition. %RSD of System, method precision, and precision at LoQ were in the range of 0.46 to 1.91. The developed method was sensitive, specific, and precise in estimating the trace levels of glyoxylic acid in emtricitabine.

Ni/Graphene Coating for Enhanced Corrosion Resistance of Metal Foam Flow Field in Simulated PEMFC Cathode Environment.

Metal foam flow field suffers serious corrosion issues in proton exchange membrane fuel cells due to its large surface area. Ni and Ni/graphene coatings are prepared under constant and gradient current modes, respectively, to improve the corrosion resistance. The effect of the electrodeposition current mode and the deposition mechanism is studied. Compared with Ni coating, Ni/graphene coating brings low corrosion current density and high coating resistance, effectively enhancing the stability of Ni foam in an acidic environment. Different from Ni coating with a single layer, Ni/graphene deposits have core-shell structure, with graphene coated on the surface of Ni nanoparticles. It is shown that graphene deposits cover the Ni particles during the electrodeposition, which protects nickel particles from agglomeration and forms an inert film on the surface of the porous structure. After an 8 h constant potential test, no significant pitting is observed on the surface of Ni/graphene coating, showing excellent anticorrosion performance. As to the effect of the deposition current mode, it is shown that more composite particles deposit on the upper layer under the gradient current mode, which brings denser protective film and fewer surface defects on the surface. Ni/graphene coating electrodeposited under a gradient current mode between 0 and 10 mA·cm-2 exhibits the lowest corrosion current densities. The values at 50 and 80 °C are only 62.9 and 26.0% of those of uncoated Ni foam, respectively.

Sustainable HPLC technique for measurement of antidiabetic drugs: Appraisal of green and white metrics, content uniformity, and in vitro dissolution

Abstract Green chemistry and white chemistry are two approaches to chemistry that prioritize sustainability and environmental protection. The pursuit of green chemistry is to develop chemical processes and products that decrease or stop the use and generation of dangerous materials. In contrast, white chemistry focuses on developing energy-efficient, sustainable chemical processes that produce minimal waste. Our study evaluated the environmental friendliness of the suggested approach, using eight greenness appraisal techniques, including analytical eco-scale, analytical method volume intensity, HPLC-environmental assessment tool, analytical method greenness score, analytical greenness, analytical greenness metric for sample preparation, green analytical procedure index, and complexgreen analytical procedure index, in addition to the unique metric blue applicability grade index of white chemistry. We have developed and validated a highly effective and reliable method for concurrently analyzing designated pharmaceutical medications characterized in metformin (MET) and empagliflozin (EMP) formulations, including their degraded products. This method is cost-effective, specific, and environmentally friendly, utilizing reversed-phase high-performance liquid chromatography with an XBridge BEH C8 column (150 mm × 4.6 mm, 5 μm) at a flow rate of 1.0 mL·min−1, an injection volume of 5.0 μL, a column oven temperature of 50°C, a wavelength of 224 nm, and a mobile phase comprised of phosphate buffer adjusted at pH 6.8 and acetonitrile in gradient mode. In the HPLC method, linearity has been achieved over the concentration range of 10–106 and 30–1,050 µg·mL−1 for EMP and MET, respectively, with correlation coefficients more than 0.999 and good recoveries within 98–102%. An assessment of the content uniformity of finished products confirmed that they met the declaration’s acceptance standards (85–115%). A comparative study has been successfully conducted on generic and reference products, demonstrating their similarity. The suggested approach was validated by adhering to international council for harmonisation criteria.

Analytical method development and validation for the evaluation of related substances in Apalutamide by RP-HPLC

Apalutamide is an anti-cancer agent used for the management of prostate cancer. A new stability indicating RP-HPLC method (Gradient mode) has been developed for the estimation of Apalutamide and its related substances using Waters Alliance system (Model no. 2996 and 2695) with Inertsil ODS-3 (250 × 4.6 mm, 5μm) column (PDA detector) was used for the present study. A mixture of Ammonium phosphate buffer solution and Acetonitrile (30: 70, v/v) was used as the mobile phase for the chromatographic study (Flow rate: 1.0 mL/min; Detection wavelength: 243 nm). Stress degradation studies were performed and the method was validated as per ICH guidelines. Keywords: Apalutamide, RP-HPLC, Related substances, Impurities, Stability indicating, Validation, ICH guidelines.

Ti/Te effects on transport in EAST low q95 plasmas

Enhanced confinement is observed in EAST low q95 ( q95<3.5 ) plasmas with the temperature ratio ( Ti/Te ) increasing. A statistically significant positive correlation between H98y2 and Ti/Te has been established. Increased Ti triggerd by Neutral Beam Injection in experiments maintaining constant Te leads initially to an improved Ti/Te ratio, which subsequently mitigates heat transport in the ion channel. The Trapped Gyro–Landau Fluid transport model was applied to analyse the dependence of the turbulent transport on Ti/Te . Simulation results demonstrate that the Ion Temperature Gradient mode is stabilized in high Ti/Te plasma, with ion flux results from predictive modelling further validating this effect. These findings corroborate the Weiland model and complement one another. This study contributes to a deeper understanding of how the dimensionless parameter Ti/Te influences plasma transport, and helps demonstrate the feasibility of operations under the low q95 condition required for the ITER-inductive scenario in the new Ti/Te regime.

Ceftobiprole quantification in human serum by HPLC-UV to implement routine TDM in clinical practice

Background and aimsCeftobiprole is a recent 5th generation parenteral cephalosporin with antibacterial activity against a large range Gram+ and Gram− bacteria. Therapeutic drug monitoring (TDM) is an essential tool for maintaining plasma concentrations of antibiotics above the MIC by the end of the dosing interval, thus preventing the resistant strain diffusion. TDM is already recommended for other cephalosporins, and it is a reasonable tool contributing to the safety and efficacy of these drugs. During the treatment of patients in real-life, a number of pharmacokinetic (PK) changes not normally seen in healthy volunteers can occur which can impair the pharmacokinetic/pharmacodynamic target attainment.We aimed to develop simple and rapid HPLC-UV method for determination of ceftobiprole in human serum to implement TDM in clinical practice and support PKs and pharmacokinetic/pharmacodynamic (PK/PD) studies. Materials and methodsSamples preparation of calibration standards, QC, and anonymous patients serum samples was performed by protein precipitation by adding 0.01 ml of sulphosalicylic acid at 30 % to 0.1 ml of each sample. Then samples were vortexed and the centrifuged at 12,000 rpm for 10 min at 4 °C. Fifty microlitres of clear supernatant were diluted 1:1 with mobile phase and transferred into HPLC autosampler held at 8 °C. Chromatographic separation was carried out in a gradient mode at 35 °C on an ultra-Biphenyl column using a Thermo Scientific chromatographic system with a Diode array. Data management was performed with Chromeleon 7.4 software. ResultsThe HPLC-UV method proved to be linear over wide concentration ranges (0.5–50.0 mg/L) and was accurate and reproducible in the absence of matrix effects, allowing for robust, specific, and rapid quantification of ceftobiprole from a low amount of serum (0.1 mL). The mean steady state Ctrough and Cend values measured in the anonymous patients’ samples were 6.26 ± 3.81 mg/L and 22.56 ± 15.69 mg/L, respectively. ConclusionsWe report a broadened simple and fast HPLC with UV detection method for quantification of ceftobiprole in human serum to implement ceftobiprole TDM as clinical routine, and support future (PK/PD) studies in special patients’ population.