Stalked Barnacle Research Articles

The goose barnacle Pollicipes pollicipes as a tool for trace metal biomonitoring and health risk assessment for human consumers in northwestern Atlantic coast of Morocco

The bioaccumulation of trace metals Cd, Cr, Cu, Fe, and Zn in soft tissues of the barnacle Pollicipes pollicipes was investigated seasonally along the Atlantic coast of northwestern Morocco. Average concentrations (μg g−1 dry weight) exhibited a decreasing order: Fe (548.15 ± 132.43) > Zn (430.80 ± 181.68) > Cd (17.46 ± 9.99) > Cu (7.72 ± 1.26) > Cr (3.12 ± 0.80), with the highest levels during wet seasons. The “Metal Pollution Index” and “Individual Multimetal Bioaccumulation Index” revealed a substantial barnacle contamination in industrialized areas. Additionally, Cd and Zn concentrations surpassed permissible guideline limits. While the “Target Hazard Quotient” and “Hazard Index” unveiled no significant health risks associated with barnacle consumption for humans, Cd posed potential risks, particularly for children consuming barnacles from polluted locations. Regarding the “Maximum Safe Consumption”, Cd demonstrated potential harm across all sex and age groups. These findings contribute valuable data on the safety of barnacle consumption, marking the initial assessment of such risks in Morocco. The study offers evidence of metal pollution occurrence and proposes the barnacle species as a reliable biomonitor of trace metal bioavailabilities in marine coastal areas. To our knowledge, this investigation is the first comprehensive report of metal contamination biomonitoring using barnacles from Moroccan Atlantic waters.

Under pressure: Assessing the ecological boundaries of the epipelagic goose barnacle Lepas anatifera using ocean gliders and laboratory experiments

Epipelagic barnacles have been considered good bioindicators since they are abundant and broadly distributed but with apparent tolerance restrictions to temperature and salinity, and also bioaccumulate pollutants. However, Lepas anatifera was found attached to the oceanic gliders, thriving through drastic and unreported environmental fluctuations. This study aimed to assess the resistance and oxidative stress responses of L. anatifera collected from gliders and attached to floating litter to temperature, salinity, and pressure. Barnacles withstood all tested pressure, temperature, and salinity ranges, except the extreme salt concentration. The activities of antioxidant enzymes – catalase and superoxide dismutase – were significantly increased under high temperature, high pressure, and low salinity. Malondialdehyde levels significantly increased only under high pressure. In conclusion, L. anatifera can be considered resistant organisms to extreme environmental changes. However, the instauration of oxidative stress under certain circumstances makes them vulnerable to predicted future trends in marine environments.

Parasitism of Pennella instructa on indo-pacific sailfish Istiophorus platypterus and its commensality relation with goose barnacle, Conchoderma virgatum along Eastern Arabian Sea

Parasitism of Pennella instructa on indo-pacific sailfish Istiophorus platypterus and its commensality relation with goose barnacle, Conchoderma virgatum along Eastern Arabian Sea

Fatty acids profiling of goose barnacle (Pollicipes pollicipes) tissues to evaluate nutritional quality and confirm harvesting location

In the Iberian Peninsula, illegal harvesting and mislabeling of geographic origin of goose barnacle (Pollicipes pollicipes) poses serious socio-economic risks, including food safety issues, product depreciation, and depletion of natural stocks. In the present study, we assess the fatty acid (FA) profiles of two soft tissues (the edible peduncle and the adductor muscle) of P. pollicipes originating from six locations over the Atlantic NW and W Iberian coasts of mainland Portugal to demonstrate their suitability to confirm the geographic origin of this species and shed light on the nutritional value of the edible peduncle. Random forest models exhibited high accuracy in allocating samples to their original location (91.7% for the edible peduncle and 86.7% for the adductor muscle). Moreover, the edible peduncle possessed a high nutritional quality, as revealed by the following indices: fish lipid quality (FLQ, %): 44.3–53.6; atherogenic index (AI): 0.32–0.48; thrombogenic index (TI): 0.14–0.20; and hypocholesterolemic/hypercholesterolemic ratio (HH): 2.65–4.64. Overall, the traceability approach employed in the present work can assist in the fight against illegal harvesting and the mislabeling of P. pollicipes geographic origin, thus contributing to the added value of this nutritious bioresource, along with its conservation and sustainable management in the Iberian Peninsula and elsewhere.

Gooseneck barnacle optimization algorithm: A novel nature inspired optimization theory and application

This paper introduces the Gooseneck Barnacle Optimisation Algorithm (GBO) as a novel evolutionary method inspired by the natural mating behaviour of gooseneck barnacles, which involves sperm casting and self-fertilization. GBO is mathematically modelled, considering the hermaphroditic nature of these microorganisms that have thrived since the Jurassic period. In contrast to the previously published Barnacle Mating Optimizer (BMO) algorithm, GBO more accurately captures the unique static and dynamic mating behaviours specific to gooseneck barnacles. The algorithm incorporates essential factors, such as navigational sperm casting properties, food availability, food attractiveness, wind direction, and intertidal zone wave movement during mating, creating two vital optimization stages: exploration and exploitation. Real-world case studies and mathematical test functions serve as qualitative and quantitative benchmarks. The results demonstrate that GBO outperforms well-known algorithms, including the previous BMO, by effectively improving the initial random population for a given problem, converging to the global optimum, and producing significantly better optimization outcomes.

A Benchmarking of Commercial Small Fixed-Wing Electric UAVs and RGB Cameras for Photogrammetry Monitoring in Intertidal Multi-Regions

Small fixed-wing electric Unmanned Aerial Vehicles (UAVs) are perfect candidates to perform tasks in wide areas, such as photogrammetry, surveillance, monitoring, or search and rescue, among others. They are easy to transport and assemble, have much greater range and autonomy, and reach higher speeds than rotatory-wing UAVs. Aiming to contribute towards their future implementation, the objective of this article is to benchmark commercial, small, fixed-wing, electric UAVs and compatible RGB cameras to find the best combination for photogrammetry and data acquisition of mussel seeds and goose barnacles in a multi-region intertidal zone of the south coast of Galicia (NW of Spain). To compare all the options, a Coverage Path Planning (CPP) algorithm enhanced for fixed-wing UAVs to cover long areas with sharp corners was posed, followed by a Traveling Salesman Problem (TSP) to find the best route between regions. Results show that two options stand out from the rest: the Delair DT26 Open Payload with a PhaseOne iXM-100 camera (shortest path, minimum number of pictures and turns) and the Heliplane LRS 340 PRO with the Sony Alpha 7R IV sensor, finishing the task in the minimum time.

Tarballs on the Brazilian coast in late 2022 sustain Lepas anatifera Linnaeus, 1758 (Crustacea: Cirripedia): Occurrence and risk of petroleum hydrocarbon ingestion

Since the 2019 oil spill on the northeastern coast of Brazil, oil materials have washed up on the beaches. A characteristic of the recent oil spill that began in late August was that some of the oiled material, such as tarballs, contained the goose barnacle species Lepas anatifera (Cirripedia, Lepadomorpha), which is well-known for its cosmopolitan distribution and wide occurrence in the oceans. The findings of this study provide information on the occurrence and contamination of petroleum hydrocarbons in animals adhered to the surfaces of tarballs sampled from beaches in the Brazilian states of Ceará and Rio Grande do Norte, between September and November 2022. The size of the barnacles varied from 0.122 to 2.20 cm, suggesting that the tarballs had been floating in the ocean for at least a month. All groups of L. anatifera collected from the tarballs had polycyclic aromatic hydrocarbons (PAHs) present (∑21PAHs from 476.33 to 3816.53 ng g−1). In comparison to high-molecular-weight PAHs, which are primarily from pyrolytic sources, low-molecular-weight PAHs, such as naphthalene and phenanthrene, which are mostly related to petrogenic sources, were shown to be more abundant. In addition, dibenzothiophene, which is exclusive of petrogenic origin, was found in all samples (30.74–537.76 ng g−1). The aliphatic hydrocarbons (AHs): n-alkanes, pristane, and phytane were also found and displayed petroleum characteristics. These results highlight the danger of increasing the absorption of petrogenic PAHs and AHs by organisms that use tarballs as substrates. L. anatifera is a crucial component of the food chain because many animals such as crabs, starfish, and gastropods consume it.

Anthropogenic fibers and microplastics in the pelagic gooseneck barnacle Lepas (Lepas) anatifera in Capo Milazzo Marine Protected Area (Tyrrhenian Sea): A first characterization

This study provides an assessment of the efficiency of the biofouler Lepas (Lepas) anatifera Linnaeus, 1758 in capturing microplastics and microfiber particles floating in the water column. In this context, we collected pelagic gooseneck barnacles at fixed moorings in the Capo Milazzo Marine Protected Area (MPA). Fibers and fragments were found in the digestive tract of 30% of the 120 specimens collected. The ingested debris were mainly fibers (85.9%) of synthetic (30.6%) and natural (11.7%) origin, with length ranging between 1 and 2 mm (33.3%) and transparent (47.2%). The highest concentration of fibers was recorded in barnacles collected in the western sector of the MPA that is more affected by the input of organic matter coming from several urban wastewaters. No correlation was found between the presence of artificial polymers in L. (Lepas) anatifera and the size of individuals. The great diversity of fibers and plastic fragments by typology, size, shape and color, as well as the large number of bivalve and ostracod shells found in the digestive tract of the samples, confirm the opportunistic diet of these organisms, which can indiscriminately ingest any type of prey and cannot distinguish between microplastics and food. The results obtained, as well as the wide distribution and abundance, and ease of sampling of these barnacle species in macrofouling suggest that including this species in monitoring programs could be a cost-effective and easy method for assessing the presence of microplastics and microfibers in coastal marine waters to monitor the ecological status of pelagic and coastal ecosystems, including MPAs.

New identifications of natural history images on the “Bodleian Plate”, an early eighteenth-century engraved copperplate

The “Bodleian Plate”, an early eighteenth-century engraved copper printing plate, bears a series of images of buildings erected in Williamsburg, Virginia, USA, as well as depictions of plants and animals. Four of these natural history images portray fragments of marine algae here identified as Sargassum spp. (Sargassaceae) and Ascophyllum nodosum (Fucaceae). A fifth botanical subject is identified as fragments of the inflorescences of Tilia sp. (Malvaceae). Two of the Sargassum fragments show commensal marine organisms, a bryozoan (probably Jellyella tuberculata (Membraniporidae)) and goose barnacles ( Lepas sp. (Crustacea; Cirripedia)). A seahorse ( Hippocampus sp. (Syngnathidae)), a ground beetle and a spider are also depicted. These new identifications contradict the hypothesis that the illustrations portray agricultural or other commodities of financial importance in Virginia during the early 1700s. A putative link with William Byrd II of Westover, Virginia, is not supported by the natural history images.

Structure and Composition of the Cuticle of the Goose Barnacle Pollicipes pollicipes: A Flexible Composite Biomaterial.

Arthropods, the largest animal phylum, including insects, spiders and crustaceans, are characterized by their bodies being covered primarily in chitin. Besides being a source of this biopolymer, crustaceans have also attracted attention from biotechnology given their cuticles' remarkable and diverse mechanical properties. The goose barnacle, Pollicipes pollicipes, is a sessile crustacean characterized by their body parts covered with calcified plates and a peduncle attached to a substrate covered with a cuticle. In this work, the composition and structure of these plates and cuticle were characterized. The morphology of the tergum plate revealed a compact homogeneous structure of calcium carbonate, a typical composition among marine invertebrate hard structures. The cuticle consisted of an outer zone covered with scales and an inner homogenous zone, predominantly organic, composed of successive layers parallel to the surface. The scales are similar to the tergum plate and are arranged in parallel and oriented semi-vertically. Structural and biochemical characterization confirmed a bulk composition of ɑ-chitin and suggested the presence of elastin-based proteins and collagen. The mechanical properties of the cuticle showed that the stiffness values are within the range of values described in elastomers and soft crustacean cuticles resulting from molting. The removal of calcified components exposed round holes, detailed the structure of the lamina, and changed the protein properties, increasing the rigidity of the material. This flexible cuticle, predominantly inorganic, can provide bioinspiration for developing biocompatible and mechanically suitable biomaterials for diverse applications, including in tissue engineering approaches.

First record of the barnacle Conchoderma virgatum on an ephippid fish

AbstractThe goose barnacle Conchoderma virgatum is a highly opportunistic epibiont, with observations of attachment to a wide range of marine organisms. Attachment to perciform fishes, however, is rarely observed. Observations of Conchoderma-osteichthyan associations in general are also rare in the southern hemisphere, especially in Australian waters. We present the first record of C. virgatum as an epibiont on an ephippid fish (Platax teira). This is also only the second known record of C. virgatum attached to a perciform fish in the Tasman Sea. We also summarize all previous known observations of C. virgatum in association with perciform fishes.

Chaotic Genetic Patchiness in the Highly Valued Atlantic Stalked Barnacle Pollicipes pollicipes From the Iberian Peninsula: Implications for Fisheries Management

The stalked barnacle Pollicipes pollicipes inhabits rocky shores from the Atlantic coasts of Brittany (France) to Senegal. Because of the culinary traditions of southern Europe, stalked barnacles represent an important target species for local fisheries on the Iberian Peninsula. To manage this fishery sustainably, it is therefore important to assess the dynamics of local populations over the Iberian coast, and how they are interconnected at a wider scale using finely tuned genetic markers. In this work, a new enriched library of GT microsatellites for P. pollicipes was prepared and sequenced using Ion Torrent™ Next Gen-Sequencing Technology. 1,423 adults and juveniles were sampled in 15 localities of three geographic regions: southern Portugal, Galicia and Asturias (both in northern Spain). Twenty polymorphic loci arranged in five multiplex PCRs were then tested and validated as new molecular tools to address the spatial and temporal genetic patterns of P. pollicipes. Our results revealed high genetic diversity among adults. However, juveniles were genetically more structured than their adult counterparts, which alternatively displayed much more connectivity among the three studied regions. The lack of spatial genetic heterogeneity in adults may be due to the overlapping of several generations of settlers coming from different geographic origins, which mainly depends on the orientation of residual currents along the coast during reproduction. The genetic differentiation of juveniles may indeed be congruent with Iberian Peninsula hydrodynamics, which can produce chaotic genetic patchiness (CGP) at small temporal scales due to sweepstake reproductive success, collective dispersal and/or self-recruitment. Remarkably, most of the genetic heterogeneity of juveniles found in this work was located in Galicia, which could represent an admixture between distinct metapopulations or an old refuge for the most northern populations. To conclude, high genetic variation in P. pollicipes can lead to the false impression of population panmixia at the Iberian scale by masking more restricted and current-driven larval exchanges between regions. This possibility should be taken into consideration for further specific management and conservation plans for the species over the Iberian Peninsula.

Chromosome-level genome assembly, annotation, and phylogenomics of the gooseneck barnacle Pollicipes pollicipes.

BackgroundThe barnacles are a group of >2,000 species that have fascinated biologists, including Darwin, for centuries. Their lifestyles are extremely diverse, from free-swimming larvae to sessile adults, and even root-like endoparasites. Barnacles also cause hundreds of millions of dollars of losses annually due to biofouling. However, genomic resources for crustaceans, and barnacles in particular, are lacking.ResultsUsing 62× Pacific Biosciences coverage, 189× Illumina whole-genome sequencing coverage, 203× HiC coverage, and 69× CHi-C coverage, we produced a chromosome-level genome assembly of the gooseneck barnacle Pollicipes pollicipes. The P. pollicipes genome is 770 Mb long and its assembly is one of the most contiguous and complete crustacean genomes available, with a scaffold N50 of 47 Mb and 90.5% of the BUSCO Arthropoda gene set. Using the genome annotation produced here along with transcriptomes of 13 other barnacle species, we completed phylogenomic analyses on a nearly 2 million amino acid alignment. Contrary to previous studies, our phylogenies suggest that the Pollicipedomorpha is monophyletic and sister to the Balanomorpha, which alters our understanding of barnacle larval evolution and suggests homoplasy in a number of naupliar characters. We also compared transcriptomes of P. pollicipes nauplius larvae and adults and found that nearly one-half of the genes in the genome are differentially expressed, highlighting the vastly different transcriptomes of larvae and adult gooseneck barnacles. Annotation of the genes with KEGG and GO terms reveals that these stages exhibit many differences including cuticle binding, chitin binding, microtubule motor activity, and membrane adhesion.ConclusionThis study provides high-quality genomic resources for a key group of crustaceans. This is especially valuable given the roles P. pollicipes plays in European fisheries, as a sentinel species for coastal ecosystems, and as a model for studying barnacle adhesion as well as its key position in the barnacle tree of life. A combination of genomic, phylogenetic, and transcriptomic analyses here provides valuable insights into the evolution and development of barnacles.

Biogeochemistry and trophic structure of a cold seep ecosystem, offshore Krishna-Godavari basin (east coast of India)

Active cold seep sites associated with shallow gas hydrates were recently reported from Krishna-Godavari (K-G) basin, off east coast of India. Benthic fauna including chemosymbiotic bivalves (genera Bathymodiolus, Acharax, Conchocele), polychaetes (genus Sclerolinum), and heterotrophs (gastropods: Provannidae family; squat lobster: Munidopsidae/Galatheidae; Goose barnacle: genus Neolepas; Brittle star: genus Amphiodia) were sampled from the cold seep locations. Here, we present the first δ13C and δ15N data sets of the soft tissues extracted from the organisms along with the sediment pore fluid composition. The isotope ratios of the soft tissues provide a glimpse of the potential food sources for the cold seep organisms and the trophic structure of the ecosystem. The δ13C values suggest the dependence of the Bathymodiolus on methanotrophic symbionts, whereas Acharax, Conchocele, and Sclerolinum show the tell-tale signature of the thiotrophic pathways. In contrast, the heterotrophic benthic fauna shows a wide range of δ13C values, a signature of a variety of carbon sources. The δ15N values of the studied organisms also show a broad range (−1 to +9 ‰) indicating variable food sources as well as variable nitrogen assimilation pathways followed by various symbionts.

Marine turtles and their barnacles from Scottish waters and adjacent seas

The history of marine turtles in Scottish waters and adjacent seas is reviewed. The recording and reporting schemes for stranded marine animals in Scotland and the U.K. are highlighted. Detailed descriptions are provided of strandings of a loggerhead turtle (Caretta caretta) at Irvine, Ayrshire in December 2015, with numerous attached goose barnacles (Lepas anatifera) and a leatherback turtle (Dermochelys coriacea) at St. Cyrus, Aberdeenshire in January 2016, with several acorn barnacles (Stomatolepas dermochelys). A review of U.K. marine animal strandings reports revealed additional records of L. anatifera and L. hillii, from stranded loggerheads in Carmarthenshire in 2006 and in Cornwall in 2016 respectively, as well as another leatherback with S. dermochelys in Cornwall in 2018. The occurrence of lepadomorph and balanomorph barnacles on turtles is discussed including historical records from the U.K. and Ireland, and adjacent waters in the Mediterranean and the north-east Atlantic. Taxonomic confusion within the genus Stomatolepas is clarified. Other species of barnacle that might occur on turtles reaching the waters of the U.K. and Ireland in the future are discussed along with other exotic fauna that can accompany turtles. The impact of barnacles on turtles and how turtles remove attached epibionts is summarised.

An example for transatlantic hitchhiking by macrozoobenthic organisms with a research vessel

In 2019 the RV Meteor cruised from Guadalupe in April/May to Cape Verde in June/July and to Namibia in August/September. The distance is about 10,000 km. The ship has a moon pool for installation of instruments. In Cape Verde we had a first glimpse of the already sparsely populated moon pool. We reached Namibian waters in mid-August. In mid-September, 47 days later and 6000 km south, the ship's moon pool was sampled in the port of Walvis Bay. 13 different taxa could be identified belonging to two phyla, four classes, six orders and 10 families. Most of these species have not yet been observed in the port or in the adjacent areas and are new records for the entire Namibian coast. The goose barnacles Conchoderma auritum (Linnaeus, 1767), Conchoderma virgatum Spengler, 1789 and Lepas anatifera Linnaeus, 1758 were particularly noticeable. They were only surpassed by the large number of amphipods. The species Ericthonius brasiliensis (Dana, 1853), Jassa marmorata Holmes, 1905, Stenothoe senegalensis Krapp-Schickel, 2015 and Paracaprella pusilla Mayer, 1890 are particularly noteworthy here. In addition, the pycnogonid species Endeis straughani Clark, 1970 and the titan acorn barnacle Megabalanus coccopoma (Darwin, 1854) should be mentioned, which occurred very frequently as well. The present study shows, on the one hand, an example of the transatlantic spread of bioinvasive species by ships as vectors and, on the other hand, a convenient method for sampling ship hulls.

Genomic insights into the sessile life and biofouling of barnacles (Crustacea: Cirripedia)

Members of the infraclass Cirripedia, commonly called barnacles, are unique among the subphylum Crustacea in that they exhibit a biphasic life cycle with a planktonic larval stage and a sessile adult stage. Understanding their unique sessile life and mechanisms of attachment are hampered by the lack of genomic resources. Here, we present a 746 Mb genome assembly of Lepas anserifera – the first sequenced stalked barnacle genome. We estimate that Cirripedia first arose ~495 million years ago (MYA) and further diversified since Mesozoic. A demographic analysis revealed remarkable population changes of the barnacle in relation to sea-level fluctuations in the last 2 MYA. Comparative genomic analyses revealed the expansion of a number of developmental related genes families in barnacle genomes, such as Br–C, PCP20 and Lola, which are potentially important for the evolution of metamorphosis, cuticle development and central nervous system. Phylogenetic analysis and tissue expression profiling showed the possible roles of gene duplication, functional diversification and co-option in shaping the genomic evolution of barnacles. Overall, our study provides not only a valuable draft genome for comparative genomic analysis of crustacean evolution, but also facilitates studies of biofouling control.

Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle Lepas anatifera.

We focus on the stalked goose barnacle L. anatifera adhesive system, an opportunistic less selective species for the substrate, found attached to a variety of floating objects at seas. Adhesion is an adaptative character in barnacles, ensuring adequate positioning in the habitat for feeding and reproduction. The protein composition of the cement multicomplex and adhesive gland was quantitatively studied using shotgun proteomic analysis. Overall, 11,795 peptide sequences were identified in the gland and 2206 in the cement, clustered in 1689 and 217 proteinGroups, respectively. Cement specific adhesive proteins (CPs), proteases, protease inhibitors, cuticular and structural proteins, chemical cues, and many unannotated proteins were found, among others. In the cement, CPs were the most abundant (80.5%), being the bulk proteins CP100k and -52k the most expressed of all, and CP43k-like the most expressed interfacial protein. Unannotated proteins comprised 4.7% of the cement proteome, ranking several of them among the most highly expressed. Eight of these proteins showed similar physicochemical properties and amino acid composition to known CPs and classified through Principal Components Analysis (PCA) as new CPs. The importance of PCA on the identification of unannotated non-conserved adhesive proteins, whose selective pressure is on their relative amino acid abundance, was demonstrated.

The ecology of Lepas-based biofouling communities on moored and drifting objects, with applications for marine forensic science

Pelagic biofoulers such as barnacles or bryozoans settle and raft on natural debris like pumice or seeds. Recent influxes of marine debris into the world’s oceans, especially plastic, have increased habitat availability for these biofoulers. Goose barnacles in the genus Lepas are some of the most common biofouling taxa globally, and play an important role in biofouling communities as foundation species. We examined community succession, growth rates and isotopic composition in Lepas and their associated biofouling communities in coastal waters of eastern Australia. Community succession on a fixed surface mooring showed an increase in species diversity over 25 weeks. Using the abundances of L. anserifera, L. anatifera, and the amphipods Caprella danilevskii and Jassa slatteryi, we created an equation to estimate minimum duration at sea. Predators such as the polychaete Amphinome rostrata may influence the biofouling community, as can beach scavengers once floating debris is cast ashore. We report a new maximum growth rate for L. anserifera of 1.45 mm−1, and our study is the first to report growth rates for any species of Lepas faster than 1 mm day−1. Lepas were larger on moored floats than on smaller, free-floating drifters. δ18O content of Lepas shells was a robust predictor of sea surface temperatures during formation for L. anatifera and L. anserifera. Our findings have important applications for estimating drift duration and trajectories of marine debris.

Possible Transoceanic Rafting of Lepas Spp. on an Unopened Plastic Bottle of Chinese Origin Washed Ashore in Victoria, Australia

Floating marine debris and litter act as a vector transporting various species across long distances. The present study reports possible transoceanic rafting of a small colony of barnacles on an unopened plastic bottle of Chinese origin found washed ashore on the Ninety Mile Beach in Victoria, Australia. The crustaceans attached were identified to be the goose barnacle Lepas pectinata. Based on the number and size of the colony the marine pollutant was estimated to adrift for several months. We hypothesised the origin of the flotsam, especially the barnacles and how it made its way from the Pacific to be washed ashore in Australia. Furthermore, we identified two types of microbes, Vibrio alginolyticus and Vibrio parahaemolyticus, associated with the Lepas pectinata growing on the bottle. This study appears to be the first report of possible transoceanic rafting on unused plastic pollutants and highlights the potential environmental threats caused by plastic.