Good Fluorescent Probes Research Articles

A novel “on-off-on” fluorescent sensor based on TSPP-Ag+ for S2− detection in real samples

As a new fluorescence sensor for S[Formula: see text]detection, TSPP-Ag[Formula: see text]-based “on-off-on” switches were designed. 5,10,15,20-(4-sulphonatophenyl) porphyrin (TSPP) is used as a good fluorescent probe due to its high quantum yield, good water solubility and good biocompatibility. At room temperature, the TSPP releases the optimal fluorescence emission wavelength at 642 nm when the excitation wavelength is set to 515 nm. This indicates the “turn on” mode and the color of the solution demonstrated is pink at this time. The strong fluorescence of TSPP can be firstly quenched by Ag[Formula: see text] as it can chelate with N atoms in the TSPP cavity, which causes the fluorescence intensity to become weak and the color of the solution changes from pink to light green. At this point, the fluorescence signal for the study system is in the “turn-off” state. When S[Formula: see text]are added to the TSPP-Ag[Formula: see text] sensing system, the fluorescence signal is recovered again due to the generation of silver sulfide (Ag2S), correspondingly, the solution color changed from light green to yellow-black. At this point, the fluorescence emission for the study system is in the “turn-on” state. Moreover, TSPP-Ag[Formula: see text] showed good selectivity and sensitivity towards S[Formula: see text]than other metal ions, and the limit of detection (LOD) is lowererd to 1.77 [Formula: see text]M, the linear range is 0 - 29.21 [Formula: see text]M. In addition, the concentration of S[Formula: see text]is detected in rabbit serum. Both of the recoveries and RSDs are within satisfactory range, which indicates that the promoted “on-off-on” fluorescent sensor can be applied to detect S[Formula: see text]in real samples.

Sol-gel synthesis of CaYAlO4:Tb phosphors and their application in detecting nitroaromatic compounds

• CaYAlO 4 :Tb was synthesized by a citrate acid assisted Pechini sol gel approach. • CaYAlO 4 :Tb emits stable red emission under irradiation of ultraviolet in different conditions. • CaYAlO 4 :Tb are good fluorescent probe of p -NT, 1,2-DNT and 4-NT. • The quenching mechanism is of a dynamic quenching one based on inner filter effect. Nitroaromatic compounds are highly toxic, carcinogenic, explosive, and refractory, they do great harm to human health, national security and natural ecosystem, so detecting trace amount of nitroaromatic compounds with high accuracy is crucial to the effective emission control and pollutant treatment. Herein, we synthesized CaYAlO 4 :Tb phosphors by citric acid assisted Pechini sol–gel approach. The samples were characterized by X-ray diffraction (XRD) Fourier transformation infrared (FTIR) spectroscopy, scanning electronic microscopy (SEM), laser particle analyzer, and photoluminescence (PL). The structure and morphology characterizations indicate the samples well crystallize with a K 2 NiF 4 structured CaYAlO 4 at 1100 °C and the samples show irregular morphology with smooth surface and a grain size of about 300 nm. Under irradiation of ultraviolet, CaYAlO 4 :Tb shows the characteristic emission of Tb(III), dominated by the 5 D 4 - 7 F 5 emission at 548 nm. The emission of CaYAlO 4 :Tb can be selectively quenched by nitroaromatic compounds such as p -nitrophenol, 1,2-dinitrobeneze and 4-nitrotoluene. The quenching effect (I 0 /I) can be well fitted into a linear equation, which make CaYAlO 4 :Tb an excellent candidate to detect nitroaromatic compounds. The quenching mechanism is determined to be a dynamic quenching process.

Applications of fluorescent biosensors based on quantum dots

In modern analytical sciences, fluorescent biosensors are widely used because of their have high sensitivity, high selectivity and stability. Quantum dots (QDs) are a kind of new semiconductor materials which are popular in recent years, which have been widely used in bioimaging, sensing, drug delivery and photocatalysis. QDs have unique and excellent photoelectric characteristics, and are mainly composed of II-VI period element. Due to its good biocompatibility, high stability, excellent water solubility and easy surface modification, the prepared QDs come from a wide range of sources and are considered to be good fluorescent probes for the development of a diverse of fluorescent biosensors. This research describes the progress of fluorescent biosensors based on QDs in recent years, focusing on the three mechanisms and applications in detection, such as the detection of glucose, uric acid and cell apoptosis. And this research also looks forward to the future development trend of QDs-based fluorescent biosensors.

Facile Synthesis Strategy from Sludge-Derived Extracellular Polymeric Substances to Nitrogen-Doped Graphene Oxide-Like Material and Quantum Dots.

Extracellular polymeric substances (EPS) are microbial aggregates derived from waste sewage sludge accumulated in sewage treatment plants, which provides natural, renewable, and abundant carbon, nitrogen, oxygen sources for the development of carbon materials to achieve the value-added utilization of waste sewage sludge resources. In this work, a nitrogen-doped graphene oxide (GO)-like material (N-GO) was simply produced using EPS as starting materials. A facile H2O2 oxidation-assisted method (room temperature) was developed to synthesize nitrogen-doped GO-like quantum dots (N-GOQDs) with strong tunable fluorescence from N-GO for the first time. This approach eliminates the conventional use of toxic chemicals, concentrated acids as well as expensive equipment, and strict condition requirements, which provides new insights into the synthesis of N-GO and N-GOQDs. In addition, this H2O2-assisted method was further demonstrated to prepare yellow fluorescent GO quantum dots (GOQDs) from GO successfully. The as-prepared N-GO shows excellent adsorption capacity for removing organic matters (malachite green, rhodamine B, and methylene blue) from water in 10 min. The water-soluble N-GOQDs were demonstrated to be a low toxicity and good biocompatibility fluorescence probe for bioimaging.

A Novel Fluorescent 1,10‐Phenanthroline‐2,9‐dicarboxaldehyde‐ 2,5‐diaminoterephthalicacid‐Schiff Base Polymer for Cu2+ Detection

AbstractA novel fluorescent Schiff base polymer (PTDA‐DATA‐SBP) is synthesized by amine aldehyde condensation reaction between 1,10‐phenanthroline‐2,9‐dicarboxaldehyde (PTDA) and 2,5‐diaminoterephthalicacid (DATA) as a selective fluorescent probe for Cu2+ detection. The as‐prepared PTDA‐DATA‐SBP displayed irregular nanospheres with a diameter of about 5 μm. Under the excitation of 338 nm, the PTDA‐DATA‐SBP had a characteristic emission peak at 565 nm. The PTDA groups in PTDA‐DATA‐SBP could coordinate with Cu2+ under help of carboxyl groups of DATA. Owing to the photoinduced electron transfer mechanism, the fluorescence of PTDA‐DATA‐SBP was weakened after the addition of Cu2+ in the presence of common ions. Hence, the PTDA‐DATA‐SBP was exploited as a fluorescent nanosensor for sensing Cu2+. The PTDA‐DATA‐SBP showed high selectivity and fast response towards Cu2+ even in presence of other relevant metal ions. The PTDA‐DATA‐SBP fluorescent sensor has a good linear range of 3.45 nM–8.00 μM, and the limit of detection was as low as 1.15 nM. The proposed PTDA‐DATA‐SBP sensor could also detect Cu2+ in actual samples. The work not only provided a good fluorescent probe for Cu2+ detection but also provided a feasible strategy to construct highly selective fluorescent probes.

Carbon-dot-based ratiometric fluorescent probe of intracellular zinc ion and persulfate ion with low dark toxicity.

Metal ions and anions play significant roles in biological systems and industrial processes, therefore it is important to develop good fluorescent probes to detect metal ions and anions. Here, N,O-co-doped carbon quantum dots (CDs) that could detect Zn2+ via a ratiometric fluorescence method were fabricated. The reaction between the as-prepared CDs and zinc acetate gave the composite CDs-Zn, in which fluorescence changed ratiometrically upon addition of S2 O8 2- . With change in excitation light, the emission peaks of the CDs and CDs-Zn were kept fixed while intensity changed. CDs and CDs-Zn exhibited good photostability, thermal stability, selectivity, and strong anti-interference ability. In addition, CDs and CDs-Zn displayed low dark toxicity under physiological temperatures. Ratiometric fluorescence imaging of intracellular Zn2+ and S2 O8 2- was carried out in living HeLa cells for both of these probes. Compared with reported ratiometric fluorescent hybrid nanosensors based on organic dyes and inorganic nanomaterials, the as-prepared CDs and CDs-Zn had low toxicity, and were easy to synthesize.

Construction of a new three-dimensional fluorescent probe based on BaII ions and 2,5-bis-(4-carboxy-phenylsulfanyl)-terephthalic acid

A new barium(II)-based coordination polymer, poly[half[μ7-2,5-bis-(4-carboxy-phenylsulfanyl)-terephthalic acid-κ7O1:O2:O3#3:O4#1:O4#4:O5#2:O5#4]barium(II)], [Ba(PSTP)0.5(H2O)]n (1), was synthesized through a simple solvothermal method using BaII ions and 2,5-bis-(4-carboxy-phenylsulfanyl)-terephthalic acid (H4PSTP). The asymmetric unit includes one BaII center, half of PSTP4− ligand and one coordinated water molecule. Thermal stability, IR spectroscopy, el.emental analysis, powder X-ray diffraction, solid-state photoluminescence, and the ability to detect small organic molecules as a fluorescent probe were investigated. Experimental results indicate that 2,4,6-trinitrophenol (TNP) and p-nitroaniline (4-NA) have a strong quenching effect on the fluorescence of 1 (dispersed in DMF solution), and the quenching rate is as high as 99.7% and 99.2%, respectively. The quenching constant Ksv is 9.6 × 105 M−1 and 5.4 × 105 M−1, respectively. The calculated LOD (limit of detection) values are 0.56 ppm (for 4-NA) and 0.31 ppm (for TNP), respectively. It can be used as a good fluorescent probe to detect TNP and 4-NA effectively with high selectivity and high sensitivity.

Mitochondrial directed ratiometric fluorescent probe for quantitive detection of sulfur dioxide derivatives

A good biocompatibility fluorescence probe for imaging of sulfur dioxide derivatives in cancer cells.

A lysosome-targetable turn-on fluorescent probe for the detection of thiols in living cells based on a 1,8-naphthalimide derivative

Biological thiols, like cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play crucial roles in biological systems and in lysosomal processes. Highly selective probes for detecting biological thiols in lysomes of living cells are rare. In this work, a lysosome-targetable turn-on fluorescent probe for the detection of thiols in living cells was designed and synthesized based on a 1,8-naphthalimide derivative. The probe has a 4-(2-aminoethyl)morpholine unit as a lysosome-targetable group and an acrylate group as the thiol recognition unit as well as a fluorescence quencher. In the absence of biothiols, the probe displayed weak fluorescence due to the photoinduced electron transfer (PET) process. Upon the addition of biothiols, the probe exhibited an enhanced fluorescence emission centered at 550nm due to cleavage of the acrylate moiety. The probe had high selectivity toward biothiols. Moreover, the probe features fast response time, excitation in the visible region and ability of working in a wide pH range. The linear response range covers a concentration range of Cys from 1.5×10−7 to 1.0×10−5mol·L−1 and the detection limit is 6.9×10−8mol·L−1 for Cys. The probe has been successfully applied to the confocal imaging of biothiols in lysosomes of A549 cells with low cell toxicity. Furthermore, the method was successfully applied to the determination of thiols in a complex multicomponent mixture such as human serum, which suggests our proposed method has great potential for diagnostic purposes. All of such good properties prove it can be used to monitor biothiols in lysosomes of living cells and to be a good fluorescent probe for the selective detection of thiols.

Thermal diffusivity and lifetime studies of Styryl 7 dye on DNA-CTMA complex

This study reports the sensing of micro viscosity variation of Styryl 7(LDS 750) dye in different solvents and biological samples through thermal lens and fluorescence lifetime techniques. Fluorescence properties of Styryl 7 dye in different solvents and with DNA-CTMA (deoxyribonucleic acid attached with cetyltrimethyl-ammonium cationic surfactant molecules) were measured using fluorescence lifetime technique. Fluorescence lifetime of Styryl 7 in different solvents are found to increase with the viscosity of the samples. With increase in weight percentage of DNA-CTMA, the fluorescence lifetime increases from 0.17ns to1.47ns. Lifetime studies confirm that Styryl 7 dye is a good fluorescent probe for detecting the micro viscosity of the samples. Thermal lens technique, which is an efficient probe to measure non-radiative relaxation route were used to measure the thermal diffusivity of Styryl 7 in different solvents and with varying weight percentage of DNA-CTMA. Thermal diffusivity of the samples are found to decrease with increase in solvent viscosity and increased weight percentage of DNA-CTMA

Effect of DNA-CTMA complex on optical properties of LDS 821 dye

We have investigated the fluorescence behavior of LDS 821 dye (Styryl 9 M) with deoxyribonucleic acid attached with cetyltrimethyl-ammonium (DNA-CTMA). Optical absorption studies confirm the intercalation of the dye molecules with DNA-CTMA. Fluorescence studies show an enhancement of fluorescence intensity of dye with DNA-CTMA, which suggest the reduction of TICT states of the dye molecule. The FWHM of the fluorescence spectrum increases from 95 nm to 161 nm indicating the formation of new energy levels when DNA-CTMA forms a complex with LDS 821 dye. Fluorescence lifetime measurements shows that lifetime of LDS 821 varies from 507ps to 953 ps with the addition of DNA-CTMA, which also confirms the deactivation of TICT states of dye molecule. Results show that the incorporation of DNA-CTMA with LDS 821 dye improves the optical characteristics of LDS 821 dye and therefore, can be used as a good fluorescence probe for DNA visualization as well as in lasing applications.

Hydrothermal synthesis of fluorescent carbon dots from sodium citrate and polyacrylamide and their highly selective detection of lead and pyrophosphate

Fluorescent carbon dots (CDs) have been a promising star in analytical and environmental fields. Present study designed a new bright-blue fluorescent carbon dots with sodium citrate and polyacrylamide by a hydrothermal method. The obtained CDs had an average diameter of 2.4 nm and exhibited excitation-independent property. The experimental results demonstrated that the bright-blue fluorescent CDs exhibited “off-on” property with Pb2+ and pyrophosphate (PPi), which proved that it was a good fluorescent probe for the determination of Pb2+ and PPi. The fluorescence intensity of CDs was significantly quenched by Pb2+ (turn-off) through forming CDs/Pb2+ complexes via an inner filter effect, and the fluorescence intensity of CDs/Pb2+ system was completely resumed by PPi (turn-on) owing to the release of CDs from the CDs/Pb2+ complexes due to higher binding force of PPi to Pb2+. The detection limits were 4.6 nM for Pb2+ and 54 nM for PPi, respectively. The probe was successfully validated with real water samples and human urine. The results showed that this the probe was facile, rapid and exhibited high sensitivity, selectivity, repeatability and stability. This “off-on” fluorescent probe based on CDs provided a promising platform for environmental and biological sensing applications.

A Schiff base fluorescence probe for highly selective turn-on recognition of Zn2+

A simple Schiff base CTS, synthesized between 2-hydroxy-1-naphthaldehyde and 2-benzylthio-ethanamine, was found to be a good turn-on fluorescence probe for the detection of Zn2+, due to the restriction of the rotation of the bond between CN and naphthalene ring and/or the blocking of the photo-induced electron transfer (PET) mechanism of the nitrogen atom to naphthalene ring. Excellent selectivity for Zn2+ was evidenced, over many other competing ions, including Fe3+, Cr3+, Ni2+, Co2+, Fe2+,Mn2+, Ca2+, Hg2+, Pb2+, Cu2+, Mg2+, Ba2+, Cd2+, Ag+, Li+, K+, and Na+, in EtOH/HEPES buffer (95:5, v/v, pH=7.4). It was noteworthy that Cd2+ had no interference with Zn2+. The stoichiometric complex of CTS-Zn2+ was determined to be 2:1 for CTS and Zn2+ in molar, based on the Job plot and single crystal X-ray diffraction data. The binding constant of the complex was 85.7M−2 with a detection limit of 5.03×10−7M. The fluorescence bio-imaging capability of CTS to detect Zn2+ in live cells was also studied. These results indicated that CTS could serve as a favorable probe for Zn2+.

Theoretical investigation on ratiometric two-photon fluorescent probe for Zn2+ detection based on ICT mechanism

OPA (one-photon absorption), TPA (two-photon absorption) and fluorescence properties of a free ligand L upon coordination with Zn2+, and the regeneration with CN− were investigated in theory. According to our research, OPA spectra of ligand L show red-shift binding with Zn2+ while blue-shift with CN−. The fluorescence spectra and TPA wavelength are shifted in the same situation as those of OPA spectra. The value of TPA cross-section decreased at first, and then increased to 1813 GM for [L-Zn(CN)4]2−. Intramolecular charge transfer (ICT) mechanism was investigated by natural bond orbital (NBO) analysis. It demonstrates that L is hopeful to be a good ratiometric fluorescent probe for zinc ion detection in solution, and it can regenerate after CN− was introduced.

Highly stable organic fluorescent nanorods for living-cell imaging

Metal-free, organic-dye-based fluorescent nanorods were fabricated through a simple solvent-exchange procedure. The as-prepared nanorods exhibit low toxicity to living cells and excellent photostability. Furthermore, they are stable in solutions of various pHs and high ionic strength and in solutions with interfering metal ions. Compared with the free DPP-Br molecules in THF, these nanorods exhibit larger Stokes shift, broader absorption spectra, and greatly improved photostability. We successfully demonstrated the application of the nanorods, including their aforementioned beneficial characteristics, as a good fluorescence probe for bio-imaging.

Fluorescent N-doped carbon dots for both cellular imaging and highly-sensitive catechol detection

N-doped carbon dots (NCDs) obtained by one-step reflux treatment of C3N4 with ethane diamine possess good water dispersibility, strong visible fluorescence, low toxicity, proton adsorption capacity, and photo-induced electron-accepting/donating abilities. Based on these unique properties, we demonstrate that the obtained NCDs can be used as a good fluorescent probe for cellar imaging without any further functionalization. Further, the NCDs are combined with laccase (LAC) to form NCDs–laccase (NCD–LAC) hybrids, which are sensitive, stable, and of low cost for the precise detection of catechol and catechol derivatives.

Dansyl-labeled anionic amphiphile with a hexadecanoic carbon chain: Synthesis and detection for shape transitions in organized molecular assemblies

The probing properties of a new fluorophore-labeled anionic surfactant, sodium 16-(N-dansyl)aminocetylate (16-DAN-ACA) were investigated systematically in molecular assemblies, especially in the transitions between micelles and vesicles. 16-DAN-ACA can efficiently differentiate the two different aggregate types in mixed cationic and anionic surfactant systems. The fluorescence anisotropy of 16-DAN-ACA was found to be sensitive for directly detecting the micellar growth in micelles containing oppositely charged surfactants; both cationic cetyltrimethylammonium bromide (CTAB) systems and anionic sodium dodecyl sulfate (SDS) systems were studied. The results indicated that the 16-DAN-ACA is a good fluorescent probe for differentiating the different aggregates, and even more can be used to detect the micellar growth.

Nonlinear Optical Chemosensor for Sodium Ion Based on Rhodol Chromophore

As part of a strategy to identify good fluorescent probes based on two-photon excited fluorescence (TPEF), the sensor for sodium cation has been designed bearing a rhodol chromophore linked with an aza-crown ether. An efficient synthetic route to rhodol derivatives possessing five-membered heterocycles at position 9 and their precursors that contain xanthylium salt has been developed. The synthesis involves condensation of xanthylium salts bearing vinamidinium moiety at position 9, with phenylhydrazine derivatives as the key step. To accomplish the synthesis of derivatives bearing 1-aza-15-crown-5 and 1,10-diaza-18-crown-6, the Buchwald-Hartwig reaction has been employed in the final stage. Electronic spectra of all prepared rhodols display strong absorption in the range of 450-550 nm with well-resolved vibronic bands, which maintains its fine structure in a wide range of solvents. The most intensive two-photon absorption (2PA) band in the rhodol spectrum (165 GM), located at shorter wavelengths, matches well with the short-wavelength absorption band in the linear electronic spectrum and is most probably related to the two-photon allowed electronic transition S0→S2. The influence of cation binding on one- and two-photon spectroscopic properties of rhodol linked with 1-aza-15-crown-5 via the phenylpyrazole bridge has been investigated. This probe exhibits high sensitivity and good selectivity for Na(+) in CH3CN. The mechanism involves the complexation of the Na(+) by 1-aza-15-crown-5 in the probe, which induces prominent fluorescence enhancement via quenching of electron-transfer. Interestingly, the complexation with Na(+) led to a significant increase of the 2PA band in the 750-800 nm region (corresponding to a two-photon allowed, one-photon forbidden transition) for rhodol bearing 1-aza-15-crown-5, which led to the overall enhancement of the TPEF signal (approximately an order of magnitude). Thus, a turn-on fluorescent probe for sodium ion, which does not respond to many other metal species, has been constructed.

Gambogic Acid Combined With CdTe QDs For Leukemia Cancer Cells Inhibition and Their Bio-Safety For Rat Brain

The development of new anticancer agents typically involves long research cycles, high cost and low efficiency and so on. There has been a growing public interest in the use of complementary and alternative medicine for cancer treatment and drug discovery. The emergence of nanotechnology and nanomaterials has greatly stimulated research in drug delivery and optimization. As a good fluorescence probe and simultaneously as potential drug carrier, Cadmium-Tellurium quantum dots (CdTe QDs) can optimize the use and a new potential therapy method of some drug-active compounds. Based on these considerations, we have explored the possibility to connect cysteamine (Cys) modified CdTe QDs (Cys-CdTe) to gambogic acid (GA). These functional QDs were studied for multi-functional drug delivery to investigate the synergetic effect of these composites between GA and QDs for human leukemia caner cells K562 and their drug-resistant cell line K562/A02 in vitro. Also the neurotoxicity of different drug systems for Sprague Dawley (SD) rat brain was analyzed by in vivo real-time microdialysis. The nanocomposites of GA loaded CdTe QDs were prepared and the drug releasing of GA-CdTe nanocomposites was performed at the medium of pH 6.0 and 7.4 in vitro. The cytotoxicity of GA and the nanocomposites for K562 and K562/A02 cells was studied by MTT, cell morphology, and flow cytometry. The labeling and imaging of CdTe QDs and the nanocomposites for cancer cells was performed by laser confocal fluorescence microscopy. Different drug systems were respectively injected into SD rat by vena caudalis and cerebrospinal fluid was collected by microdialysis in vivo. The neurotoxicity of different drug systems for rat brain was investigated by the changes of amino acid content in the cerebrospinal fluid. The Cys modified CdTe QDs showed the good fluorescence characteristics, with the wide excitation spectrum and narrow emission spectrum. The CdTe QDs could label in human leukemia cancer cells not only for K562 but also K562/A02 , which had the potential application in the cell imaging. Following the electrostatic attraction, surface adsorption et al, these CdTe QDs had the good capability of drug delivery with the high drug-loading and envelopment capacity. Also these GA-CdTe nanocomposites showed the pH sensitivity for the drug release and improved the drug's un-dissolvability. The GA-CdTe nanocomposites also had the fluorescence characteristics for labeling the different kinds of leukemia cancer cells. Gambogic acid play an important role in cancer therapy and also showed the sensitivity for the multidrug resistance (MDR) of K562/A02 cells in this study. The results indicated that GA-CdTe nanocomposites could significantly enhance the drug accumulation to improve the cytotoxicity and considerably overcome the multidrug resistance. GA-CdTe nanocomposites induced the arrest of G0/G1 phase to promote the cells apoptosis. The combination between GA and CdTe QDs can optimize the new potential therapeutic method for GA with the real time labeling and tracing during the disease therapy. The neurotoxicity of GA and GA-CdTe nanocomposites for rat brain was firstly explored by using the microdialysis in vivo. The results indicated that GA had the serious neurotoxicity through the concentration changes of amino acids for the rat brain. The concentration of some excitatory amino acids was remarkably increased. For the GA-CdTe nanocomposites based on CdTe QDs, the side effect of GA was visibly cut down, and the time to cause the neurotoxicity was apparently shortened. These nanocomposites own the better biocompatibility and bio-safety for the relevant cancer treatment in vitro and in vivo. This raises the promising possibility of the application of these fluorescent nanocomposites based on CdTe QDs for the target cancer therapy. The combination of GA with Cys-CdTe QDs can optimize the use and new potential multi-mode therapy of cancers. This may shed new light to exploit the potential application of the active compounds from Traditional Chinese Medicine (TCM). Disclosures:No relevant conflicts of interest to declare.

A novel dansyl-based fluorescent probe for highly selective detection of ferric ions

A novel dansyl-based fluorescent probe was synthesized and characterized. It exhibits high selectivity and sensitivity towards Fe3+ ion. This fluorescent probe is photostable, water soluble and pH insensitive. The limit of detection is found to be 0.62μM. These properties make it a good fluorescent probe for Fe3+ ion detection in both chemical and biological systems. Spike recovery test confirms its practical application in tap water samples.