Good Agronomic Characteristics Research Articles

Registration of Bread Wheat (Triticum aestivum L.) Variety Kulumsa for the Midlands of Ethiopia

Bread wheat (Triticum aestivum L.) is a crucial crop in Ethiopia, and breeders test newly developed elite lines for superiority to existing cultivars to boost national productivity. Recently, commercial wheat varieties with higher genetic gain for economic traits have been released, which outperform older varieties. One such variety is Kulumsa, which has the pedigree “PFAU/MILAN/5/CHEN/ AEGILOPSSQUARROSA(TAUS)/BCN/3/VEE#7/BOW/4/PASTOR/6/2*BAVIS#1/7/BORL14” and selection history “CMSS13B00513S-099M-099NJ-099NJ-15Y-0WGY”. It was developed and released by Kulumsa Agricultural Research Center for mid to high altitudes of wheat-growing agroecology of Ethiopia. Kulumsa has higher grain yield performance than the check and has good agronomic characteristics and medium maturing type compared to the current varieties. It consistently out-yielded other tested bread wheat genotypes over two years. Compared to Wane, Danda'a, and Lemu checks, Kulumsa demonstrated significant improvement in agronomic characteristics and enhanced yield by 60%, 62%, and 68%, respectively. Wane (30.2g), Lemu (29.6g), and Danda'a (32.7g) have lower thousand kernel weights than Kulumsa (39.6g). Kulumsa had a 31%, 21%, and 34% thousand kernel weight advantage over Wane, Danda'a, and Lemu, respectively. The new variety has a better hectoliter weight than Wane, Lemu, and Danda'a by 18%, 13%, and 11%, respectively. The newly released bread wheat varieties are moderately resistant to stem rust, and yellow rust, and comparable for leaf rust disease and Septoria with the checks Wane, Danda'a, and Lemu. Kulumsa proved to be more resistant to stem yellow and leaf rust than all currently produced varieties in the mid to high-land part of wheat-growing agroecology. It offers new hope for farmers of Ethiopia and has a white grain color with good general acceptance for bread with high quality.

Mecanismos asociados con la modificación del endospermo en maíz de calidad proteínica

Protein quality maize (QPM) combines the protein quality of the opaque-2 (o2) mutant with a vitreous endosperm. These characteristics have allowed breeding programs worldwide to produce QPM genotypes that help alleviate malnutrition of people in developing countries from Africa, Asia and Latin America with a cereal-based diet. However, the development of these materials has been inefficient due to the limited knowledge about the molecular basis of the conversion of the soft o2 endosperm into a vitreous phenotype in QPM. This conversion has been associated with an increase in small protein bodies rich in 27 kDa γ-zein, the synthesis of starch with a higher proportion of amylose and short-intermediate amylopectin chain branches that favors the compaction of the starch granules, as well as alterations in the amyloplast envelope that favors the interaction between starch granules and protein bodies. Additional studies about the mechanisms involved in the modification of the endosperm in QPM will contribute to produce materials with good agronomic characteristics and protein quality.

Selection index and agronomic characters of doubled haploid rice lines from anther culture

Abstract. Hadianto W, Purwoko BS, Dewi IS, Suwarno WB, Hidayat P. 2023. Selection index and agronomic characters of doubled haploid rice lines from anther culture. Biodiversitas 24: 1511-1517. Development of superior rice varieties requires selection of the best lines having good agronomic characters and high yields. This study aimed to select high-yielding doubled haploid rice lines obtained from anther culture through index selection and agronomic characters. The study was conducted using a single-factor randomized complete block design (RCBD) with genotype as treatment. Sixty doubled haploid lines and 5 check varieties were evaluated along with three replications, hence, the total experimental unit was 195. Analysis of variance showed that the genotypes had a significant effect on all observed characters, except for the number of productive tillers. The agronomic characters observed had a high broad sense of heritability, except for the number of productive tillers which was in the medium category. Furthermore, index selection was carried out based on productivity, the number of productive tillers, the total number of grains, as well as the percentage of filled grains. Based on the index selection, 29 lines were selected among the 60 doubled haploid rice lines tested. These lines had the range in productivity from 2.95 to 6.77 ton ha-1, the number of productive tillers from 12.3 to 20.8 tillers, the total number of grains from 135 to 275.4 seeds, the filled grain percentage from 53.23 to 87.61%, and the selection index from -1.48 to 7.88. The selected lines will be further tested at the next experimental stage in several locations to produce high-yielding, brown planthopper-resistant, and drought-tolerant rice varieties.

Source material from crosses among <I>Solanum</I> L. spp. for potato breeding

Background. Late blight (agent: Phytophthora infestans (Mont.) de Bary) is one of the most destructive diseases for potato. The climate conditions in the northwest of Russia are very favorable for annual late blight infestation. Spreading of the pathogen leads to significant harvest losses. A promising breeding strategy is to expand the genetic diversity of resistance sources.Materials and methods. Resistance of experimental hybrid clones to late blight, their ability to form tubers under long daylight conditions, and a number of agronomic traits were studied using standard methods.Results. Long-term evaluation data were obtained for original interspecies potato hybrids concerning their resistance to late blight. The hybrids were also assessed for their yield and agronomic tuber characteristics. Some of the produced interspecies hybrids demonstrated field resistance to late blight, high tuber yield, and good agronomic traits. Clones derived from some cross combinations significantly exceeded both parents in tuber yield per plant and tuber size.Conclusions. Crosses between potato cultivars and various late blight resistance sources made it possible to combine in hybrid clones the genes of wild and cultivated Andean species and Solanum tuberosum L. with different pedigrees. Productive hybrid clones with resistance to late blight and good agronomic characteristics promise to become useful material for potato breeding programs.

The Use of the Polish Germplasm Collection of Nicotiana tabacum in Research and Tobacco Breeding for Disease Resistance

The Polish germplasm collection of Nicotiana tabacum was started in the 1920s. Up to now, more than eight hundred accessions originating from different regions of the world have been gathered in the collection. It includes valuable breeding lines and obsolete cultivars, among them cytoplasmic male-sterile lines. Numerous cultivars are rich sources of features desired in tobacco breeding. Therefore, the accessions are continually characterised in terms of their various features, one of the most important of which is disease resistance. Much research is being done to explain the nature of resistance and its genetic basis. Moreover, cultivars with good agronomic characteristics are used in wide hybridisation, being recipients of resistance genes from wild species or are genetically modified with transgenes conditioning resistance. The biological diversity of cultivars also allows a proper selection of plant material for pathogen studies, while the large number of the accessions facilitates research into the conditions for long seed storage. Numerous examples of the use of Polish tobacco germplasm in research and breeding, specifically in disease resistance, have been presented in this paper.

Application of selection index for rice mutant screening under a drought stress condition imposed at reproductive growth phase

Abstract. Sarwendah M, Lubis I, Junaedi A, Purwoko BS, Sopandie D, Dewi AK. 2022. Application of selection index for rice mutant screening under a drought stress condition imposed at reproductive growth phase. Biodiversitas 23: 5446-5452. Drought is a condition of limited availability of water that can reduce rice growth and productivity, and may threaten food security. Therefore, efforts are needed to produce drought-tolerant rice genotypes. This study aimed to select drought tolerant of the M3 mutant upland rice population at the reproductive growth phase. This research was carried out in dryland at BATAN, Indonesia, from September 2020 to February 2021, using an augmented design. The study employed 360 ??genotypes of mutant upland rice and 5 check varieties, namely Salumpikit, IR20, IR64, Limboto, and Situgintung. The selection index was constructed based on multivariate analyses such as correlation, path, and Principal Component Analysis (PCA). The coefficient of the selected main component can be used as a selection index. Before selecting the character values of each genotype, the values were standardized to obtain the same degree of value. The results showed that grain weight per hill (W.G.), panicle density (P.D.), panicle length (P.L.), and the number of filled grains per panicle (NFG) could be used as selection criteria for mutant upland rice tolerant to drought at reproductive growth phase. The selection index formula was I = (5*0.44*WG) + (2*0.41*PD) + (0.23*PL) + (0.42*NFG). Selection of genotypes of mutant upland rice based on weighted index selection resulted in 89 genotypes with high yields and good agronomic characteristics so that they could be tested in further research.

AAC Westlock Canada Prairie Spring Red wheat

AAC Westlock, an awned hard red spring wheat ( Triticum aestivum L.) cultivar, combines high grain yield and good agronomic characteristics with excellent resistance to leaf, stripe, and stem rust (including variants of Ug99), Fusarium head blight (FHB), and common bunt. Based on 40 station-years of registration trial data from 2018 to 2020, the grain yield of AAC Westlock was 1% higher than AAC Foray and 7% over AAC Penhold. AAC Westlock was significantly shorter than AAC Foray, had straw strength similar to AAC Penhold, and maturity similar to AAC Foray. AAC Westlock had similar test weight and protein concentration but lower thousand kernel weight as compared to AAC Foray. AAC Westlock had milling and baking quality suitable for grades of the Canada Prairie Spring Red wheat market class.

Determination of physiological maturity of four quinoa (Chenopodium quinoa Willd) varieties for introduction in the Russian Federation

Due to its high agricultural and nutritional potential, interest in quinoa has increased in recent years, making it a diversification alternative for agriculture in Russia. Therefore, it is necessary to have new varieties that adapt in the sowing season, with good agronomic characteristics. The quinoa a cultivated plant is an allotetraploid of 36 chromosomes, means that it has two copies of two different genomes (A and B) indicating their allelic richness, with various varieties. Therefore, it is necessary to study statistically for the determination of such phenotypic and genotypic variations in quinoa varieties. Based on this, the present research was carried out during 2021 in the experimental plot in The Federal Scientific Center for Vegetable Growing (FGBNU “FNTSO”), Moscow, Russia to study ripening of grain. Four varieties were grown in an experimental area of 40 m2 and divided into twelve experimental units. Therefore, to determine this statistical variable (from sowing to physiological ripening of grain in days), it is necessary to evaluate the germination and vegetative phases. All varieties germinated on April 20, 2021. The beginning of flowering (days) and length of panicle (cm) were also recorded in all varieties of quinoa. The NPK fertilizer was applied to all varieties at the rate of 16%/2.5 kg. The optimal grain filling was considered as the ripening stage of the grain. Duncan's Multiple Range Test was applied for the study of variations in 4 varieties of quinoa (1. Purple, 2. Lilac, 3. Green and 4. Robust green) through 4 repetitions. The results showed significant differences between varieties 1, 3 and 4. The varieties 1 (Purple) and 3 (Green) with physiological maturity at 132 and 133 days, respectively being semi early varieties, which represented 50% of plants in the experimental plots. The variety 4 (Robust green) with physiological maturity at 163 days being a late variety represented 15% of plants in the experimental plot. The introduction of the quinoa in Russia is very important where there are variations in weather during growing season. For quinoa cultivation, only 4 months are available in the spring and summer seasons in Russia. The climate begins with light rains in the month of May followed by strong radiation during June, featuring drought in the month of July, ending with the harvest in the month of August. The key factor for successful cultivation of quinoa in Russia is to adapt early varieties of quinoa of Peruvian origin.

ESTIMATION OF SALT TOLERANCE POTENTIAL OF SYNTHETIC HEXAPLOID WHEAT BASED ON ION DISCRIMINATION

Soil salinity has become a significant problem in agricultural lands making a huge area unavailable for production. Salt affected lands can be exploited effectively by utilization of salt tolerant germplasm for various crops through identification and development of tolerant genotypes. Wheat being a major food crop in many countries come to priority in this regard, and has been subjected to extensive breeding to develop tolerant germplasm. In the quest for novel sources of genes to improve the existing germplasm, several synthetic hexaploids have been developed by exploiting a diploid wheat progenitor, Aegilops tauschii. Some of these synthetic hexaploids were evaluated for their salt tolerance potential using an in vitro test called K+:Na+ discrimination. A total of thirteen genotypes were grown in hydroponics at a salinity level of 75mM NaCl. Eight genotypes showed tolerance by discriminating between Na+ and K+ ions under saline conditions. SH-13 and SH-11 were the most tolerant genotypes with good agronomic characters under salt stress, while SH-3, SH-5, SH-7, SH-8, and SH-12 showed the highest 1000 grain weight and more grains per spike in the field. A positive correlation was found between root and shoot length and K+/Na+ value of the tolerant genotypes. The results suggested that the genes for salt tolerance have been transferred in the synthetics from wild species which can be exploited through their utilization in wheat breeding programs.

Evaluasi Galur-galur Padi Sawah Dihaploid Hasil Kultur Antera Menggunakan Seleksi Indeks

Plant breeders performed multi-trait selection using a suitable method, one of which is selection index. A multivariate analysis may be used for developing a selection index model. The objective of this experiment was to select doubled haploid rice lines with high yield potential and good agronomic characters based on the selection index. The experiment was carried out using a single factor completely randomized design with three replications, from August 2018 to December 2019. The study included 89 doubled haploid (DH) lines derived from anther culture as well as four check varieties (Ciherang, Inpari 30 Ciherang Sub1, Inpari 41, and Inpari 35 Agritan). Development of selection index model considered the results of analysis of variance, correlation analysis, path analysis, principal component analysis, and aimed plant ideotype. The findings revealed that there was variation in agronomic traits and yield among the DH lines tested. The selection index model can be formulated as I (index value) = 3*0.75 yield + 0.85 total grain per panicle – 0.85 plant height. Based on this model, 85 lines had index value and yield higher than Inpari 41. The selected DH lines were included in the subsequent field evaluation.
 Keywords: correlation, heritability, path analysis, variability

Yield Performance Evaluation of Arabica Coffee Progenies Resulted from Three Way Cross Method

Breeding for high yielding, pest and disease resistance, and good quality Arabica coffee is the direction of the coffee breeding program in Indonesia. Threeway cross-breeding method becomes the alternative method to improve plant genetics to achieve those goals. This research was aimed to evaluate the agronomic performance and grouping of Arabica coffee genotypes from three-way cross progeny. This study was conducted in Andungsari Experimental Station, Bondowoso, East Java from July until September 2021. A randomized complete block design with three replications was used as an experimental design. It consisted of eight Arabica genotypes resulting from three-way cross method and one check variety (Andungsari 1) as treatments. The tested genotypes were resulted from crossing parents i.e. S 1934, AB 3, RC, YC, S 795, and Catimor that was planted in 1997. The results of the agronomic performance evaluation showed that TWC (Tree Way Cross) 2 ((S 1934/AB 3 x AB 3) x AB 3) and TWC 3 (RC/S 795 x Catimor) had good agronomic characteristics i.e. weight of 100 cherries and cherry weight per tree. While grouping using heatmap clustering method showed that there were three groups of genotypes. One of the groups which consisted of TWC 1 ((S 1934/AB 3 x AB 3) x S 1934), TWC 2 ((S 1934/AB 3 x AB 3) x AB 3), and TWC 5 (S 1934/YC x S 1934) were categorized as moderate yield group.

Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes

Conservation of genetic resources is essential for the continuation of future crop production. Watermelon (Citrul-lus lanatus), a member of Cucurbitaceae, is widely distributed in tropical and subtropical regions. The aim of this study was to reveal the genetic relationships with the help of microsatellite markers in a watermelon collection free of unnecessary repetitions, and to determine the success of SSR (Simple Sequence Repeats) primers developed in cucurbits. In this study, 96 watermelon genotypes with good agronomic characteristics were used among the geno types collected from different regions of Turkey and purified up to the S4-S6 (self-pollination) stage. In the study, 33 SSR primer pairs were used to determine the genetic relationship between watermelon genotypes. In the study, a total of 67 bands were obtained with SSR primers. As a result of UPGMA (Unweighted Pair Group Method Using Arithmetic Averages) analysis, genotypes showed similarity at the level of 0.84-1.00. The number of alleles detected per primer varied between 1 and 6. In terms of the total number of alleles obtained, CMCT44 (5 units) and Cgb4767 (6 units) loci produced the most alleles. Primers with high polymorphism rate and allele excess were determined, and the possibilities for use in genetic stability analyses, variety differentiation and other genetic analyses were determined.

Characterization of Biochar Derived from Crop Residues for Soil Amendment, Carbon Sequestration and Energy Use

The crop residues generated in agricultural fields are mostly considered a burden due to their disposal issues. This study attempts to effectively use pigeon pea stalk (PPS) for biochar production, a promising source as a soil amendment for carbon sequestration and alternative fuel source. PPS was pyrolyzed at different loads and reaction times to optimize the kiln temperature (350–400 °C and 450–500 °C) and changes in physicochemical properties, higher heating value (HHV) and yield were assessed. The results indicated that biochar yield, volatile matter, bulk density, O/C and H/C atomic ratios decreased, whereas fixed carbon, ash content and total porosity increased with increasing kiln temperature across all loads. Biochar produced at 450–500 °C (18 kg load kiln−1) had higher total carbon, nitrogen, phosphorous, recovered total carbon and total nitrogen, total potential carbon and CO2 reduction potential. Biochar produced at 350–400 °C had the maximum cation exchange capability (43.0 cmol kg−1). Biochar has estimated O/C and H/C atomic ratios of 0.07–0.15 and 0.35–0.50, respectively. Biochar exhibited good agronomic characteristics and fulfilled key quality criteria of H/C < 0.7 and O/C < 0.4 for soil carbon sequestration, as described by the European Biochar Certificate and the International Biochar Initiative. The estimated mean residence time and the mass fraction of carbon that would remain after 100 years were consistently greater than 1000 years and 80%, respectively. The biochar produced at 450–500 °C (at 18.0 kg kiln−1) from PPS had higher fixed carbon (65.3%), energy density (1.51), energetic retention efficiency (53%), fuel ratio (4.88), and HHV (25.01 MJ kg−1), as well as lower H/C and O/C ratios, implying that it is suitable for use as an alternative solid fuel.

AAC Whitehead hard white spring wheat

AAC Whitehead, an awned hard white spring wheat (Triticum aestivum L.) cultivar, combines high grain yield and good agronomic characteristics with excellent disease resistance. Based on 34 station-years of registration trial data from 2017 to 2019, AAC Whitehead had grain yield significantly higher than all of the check cultivars. AAC Whitehead had maturity similar to the checks, low lodging scores, and significantly shorter plant height than Snowstar and Whitehawk. AAC Whitehead had significantly lower test weight and higher kernel mass than than all the check cultivars. AAC Whitehead expressed resistance to the prevalent races of leaf, stripe and stem rust, and common bunt; and moderate resistance to Fusarium head blight (FHB). It also expressed tolerance to the orange wheat blossom midge. AAC Whitehead expresses quality attributes within the range of the check cultivars and is eligible for grades of Canada Western Hard White Spring wheat.

AAC Perform red spring wheat

AAC Perform, an awned hard red spring wheat (Triticum aestivum L.) cultivar, combines high grain yield and good agronomic characteristics with excellent resistance to leaf, stem, and stripe rust. Based on 43 station years of registration trial data from 2017 to 2019, the grain yield of AAC Perform was about 6% higher than AAC Foray and about 12% over AAC Penhold. AAC Perform was significantly shorter than AAC Foray and had straw strength similar to AAC Penhold. It was about two days later maturing than AAC Foray. AAC Perform had similar test weight, smaller kernel size and lower protein concentration as compared with AAC Foray. AAC Perform had milling and baking quality suitable for grades of the Canada Prairie Spring Red wheat market class.

AAC Castle red spring wheat

AAC Castle, an awned hard red spring wheat (Triticum aestivum L.), cultivar, combines high grain yield and good agronomic characteristics with excellent resistance to leaf, stem, stripe rust, common bunt and loose smut. It also expressed tolerance to the orange wheat blossom midge. Based on 39 station years of data in the registration trials from 2014 to 2016, the grain yield of AAC Castle was about 17% higher than 5700PR but similar to the other checks. AAC Castle was significantly shorter than AAC Foray and CDC Terrain, but had similar lodging resistance and maturity. AAC Castle had higher test weight, protein concentration, falling number and flour yield than AAC Foray and CDC Terrain. AAC Castle is eligible for grade of the Canada Prairie Spring Red wheat market class.

Greening Ultrasound-Assisted Extraction for Sorghum Flour Multielemental Determination by Microwave-Induced Plasma Optical Emission Spectrometry.

Sorghum is the fourth most important cereal produced in Argentina and the fifth worldwide. It has good agronomic characteristics and could be developed in arid areas, allowing a wide geographic distribution. Its starch content, higher than 70%, makes it possible to obtain a good yield of flours. Nutritionally, it should be noted that the grain does not have the protein fraction called prolamins, which makes it suitable for consumption by people with celiac disease. The multielemental composition constitutes an important indicator of the nutritional profile of the grains and allows, together with other parameters, to select the most suitable varieties for human consumption. In its determination, the preanalytical stage is decisive to obtain a reliable result. Organic samples are a challenge for sample introduction systems that use plasma-based techniques. As an alternative to conventional pretreatment with a microwave-assisted digestion (MWAD), a greener, quick, and simple treatment is proposed, using ultrasound-assisted extraction (UAE) in diluted acid media. The UAE method accelerates analysis times, improves performance and productivity, and was applied to sorghum samples cultivated in the province of La Pampa (Argentina). Microwave-induced plasma optical emission spectrometry (MIP OES) was employed for the determination of Cu, K, Mg, Mn, P, and Zn. The detection limits found ranged from 0.6 (Cu) to 89 (P) mg kg−1, and the precision expressed by the relative standard deviation (RSD) was ≤7.7% (Zn). For validation, a maize reference material (NCS ZC 73010) was evaluated. The principal component analysis revealed three different groupings related to the sorghum varieties' mineral profile.

Performance of Solanum phureja-derived bacterial-wilt resistant potato clones in a field naturally infested with Ralstonia solanacearum in Central Brazil

ABSTRACT Bacterial wilt (BW), or brown rot, caused by the soil and seed borne bacterium Ralstonia solanacearum, is one of the most devastating diseases of potatoes cultivated in warmer regions of the world. There are no potato cultivars with a desirable level of BW resistance, although it has been recognized that resistance can be an outstanding component for disease management. However, the sources of resistance available lack agronomic traits required by potato growers, therefore being of little interest to breeders. The objective of this work was to evaluate the performance of 11 clones selected for BW resistance and improved for tuber traits upon selection in the last two decades. The clones under test were compared with susceptible and resistant clones and cultivars, in a completely randomized blocks design with three replications of single lines of 10 plants, in a field naturally infested with race 1, biovar 1, phylotype II of R. solanacearum. BW incidence was assessed 60-70 days after planting and total tuber yield in each plot was recorded 110 days after planting. All the evaluated clones presented higher levels of resistance to BW compared with the commercial varieties, not differing from the resistant, not commercial, controls. In a next step, these clones will be characterized for other desirable traits and those which combine high level of resistance and commercial characteristics will be recommended for breeders for enriching the genotypic background in the search for commercial varieties. We also confirmed that the cultivar BRSIPR Bel displays an intermediate level of resistance, what makes it an interesting genitor for its good agronomic characteristics. The findings of this work demonstrate that the improved potato clones selected under tropical conditions in the Embrapa’s pre-breeding project possess high and stable levels of resistance to bacterial wilt, being a valuable resource for breeders.

Performance and genetic diversity of pre-commercial sweet sorghum hybrids in Central-Western and Southern Brazil

Sweet sorghum is a promising source of ethanol production. In this sense, the investigation of genetic diversity aims to direct heterotic crosses and identify populations that display transgressive segregation in advanced generations. For this, two experiments consisting of 10 sweet sorghum genotypes were installed in 2015/16 at the municipalities of Sinop, in the state of Mato Grosso (MT), and Passo Fundo, in the state of Rio Grande do Sul (RS), Brazil. The variables evaluated in this assessment comprised fresh mass production, dry mass production, Ethanol liters per hectare, soluble solid contents, plant height and flowering. The sweet sorghum performance and genetic diversity was estimated. Five genetic groups were observed. Fresh mass, Ethanol, flowering and height were significant for both locations, demonstrating the influence of environment on hybrid behavior. Crosses between genotypes 4x8 at Passo Fundo, and 9x10 at Sinop are favorable, as these genotypes exhibit good agronomic characteristics, especially for the main variable employed in the sugar-alcohol industry. Flowering contributed the most to genotype separation in both locations. Crosses between genotypes 4x8 and 4x9 are promising to obtain superior segregating populations for both environments, as they exhibited the greatest diversity-interfering contributions considering the investigated variables.

Assessment of potato varieties preserving at VIR’s collection to (Phytophthora infestans) resistance for late blight in field and laboratory evaluation

Thirty-eight varieties of potato Solanum tuberosum L. from the N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) collection were studied for leaf and tuber resistance to late blight. Аn aggressive Phytophthora infestans isolate collected from VIR experimental field was used for inoculation at a concentration of 50,000 zoospores/ml. The disease severity was evaluated using a scale from 1 to 9 grades, where grade 9 means resistant. Recent field observations revealed that most of the variety accessions were sensitive to late blight. In the present study, some of the varieties identified as foliar resistant in field evaluation were further tested under artificial inoculation. Resistance of some varieties found in field observations was not confirmed in laboratory assays. The data obtained in tuber inoculation tests did not show a direct correlation between resistance levels in leaves and tubers. Only some varieties possessed both characteristics, while varieties with leaf resistance and tuber sensitivity and with tuber resistance and leaf sensitivity were also identified. To develop potato varieties that combine resistance to late blight with good agronomic characteristics, resistant lines identified in this study can be potentially hybridized with varieties of high consumer value that are susceptible to late blight.