Glyphosate Salt Research Articles

The effectiveness of different formulations of glyphosate herbicides under simulated rainfall conditions in controlling various weeds

Herbicide effectiveness is affected by herbicide formulation, rainfall, and weed type. Differences in glyphosate salt formulations and 2,4 D amine mixtures may result in variations in the herbicide's ability to wash off the herbicide due to rain. The aim of this study was to assess the effectiveness of different glyphosate herbicide formulations under varying rainfall in controlling various weeds. The experiment took place in a controlled greenhouse environment, utilizing a split plot experimental design with four replications. The main plot was assigned to six different rainfall timings: 0 hours after application (HAA), 1 HAA, 2 HAA, 3 HAA, 4 HAA, and no rainfall. The subplots involved different herbicide formulations, namely isopropylamine glyphosate (h1), potassium glyphosate (h2), sodium glyphosate (h3) and glyphosate herbicide, IPA herbicide glyphosate + 2,4 D Amine (h4) and without any herbicide application (h0). Various parameters were observed, including weed dry weight and percentage of mortality growth. The results showed that the effectiveness of each herbicide formulation was diffent among weed specie under simulated rainfall conditions. Ageratum conyzoides can be controlled using isopropylamine glyphosate and potassium glyphosate with rainfall at 1 HAA. Axonopus compressus can be controlled by isopropylamine glyphosate and sodium glyphosate with rainfall at 1 HAA, while Borreria alata and Cyperus rotundus were effectively controlled by isopropylamine glyphosate with rainfall at 1 HAA.

Features of analytical control of glyphosate salts in preparative formulations

Goal. Development of a method of analytical control of glyphosate in preparative forms of herbicides.
 Methods. Determination of glyphosate was carried out by the chromatographic method of thin-layer chromatography (TLC) using Sorbfil plates with a thin layer of silica gel KSK adsorbent. The active substance was identified by the value of Rf, quantitative determination was based on the formalized dependence of the size of the area of the chromatographic zones on the amount of glyphosate.
 Results. Physicochemical properties of herbicides — electrolytes, to which glyphosate belongs, create special problems when developing methods for its determination in various matrices. In herbicides and desiccants, glyphosate (as a salt) usually takes the form of a deprotonated acid residue and a corresponding cation. Therefore, the determination of the active substance is carried out by the acid residue, which is important for the quantitative assessment of its content in the matrix both in the acid equivalent and in the form of the corresponding salt. The density of the preparative form serves, in particular, as a criterion for controlling the form of the glyphosate salt. The method is based on dissolution of the preparation form in 44% ethanol and quantitative determination by TLC method. The determination of the active substance is not hindered by other components of the preparations, derivatives of glycine and amines.The optimal conditions of analysis were chosen using the conceptual designs of the laboratory «Algorithm of chemical-analytical monitoring of pesticides» and «System of multi-quantitative determination of pesticides in matrices», according to which the main stages of the process are extraction/dissolution, purification of extracts, elution (chromatographic process), identification and quantitative analysis . Selective conditions for TLC determination — mobile phase with pH 9: distilled water + ethanol + aqueous ammonia (22 + 20 + 0.25 by volume), developing reagent: a specific complex of ninhydrin with cadmium acetate in an acidic medium. Quantitative determination is carried out taking into account the coefficients of conversion of the corresponding salt form to the acid equivalent.
 Conclusions. The use of the chromatographic method of thin-layer chromatography makes it possible to control the content of the active substance glyphosate in various salt formulations in preparative forms of herbicides and desiccants with high accuracy and reproducibility.

Gastrointestinal Microbial Ecology of Weaned Piglets Fed Diets with Different Levels of Glyphosate.

Glyphosate possesses antimicrobial properties, and the present study investigated potential effects of feed glyphosate on piglet gastrointestinal microbial ecology. Weaned piglets were allocated to four diets (glyphosate contents [mg/kg feed]: 0 mg/kg control [CON; i.e., basal diet with no glyphosate added], 20 mg/kg as Glyphomax commercial herbicide [GM20], and 20 mg/kg [IPA20] and 200 mg/kg [IPA200] as glyphosate isopropylamine [IPA] salt). Piglets were sacrificed after 9 and 35 days of treatment, and stomach, small intestine, cecum, and colon digesta were analyzed for glyphosate, aminomethylphosphonic acid (AMPA), organic acids, pH, dry matter content, and microbiota composition. Digesta glyphosate contents reflected dietary levels (on day 35, 0.17, 16.2, 20.5, and 207.5 mg/kg colon digesta, respectively). Overall, we observed no significant glyphosate-associated effects on digesta pH, dry matter content, and-with few exceptions-organic acid levels. On day 9, only minor gut microbiota changes were observed. On day 35, we observed a significant glyphosate-associated decrease in species richness (CON, 462; IPA200, 417) and in the relative abundance of certain Bacteroidetes genera: CF231 (CON, 3.71%; IPA20, 2.33%; IPA200, 2.07%) and g_0.24 (CON, 3.69%; IPA20, 2.07%; IPA200, 1.75%) in cecum. No significant changes were observed at the phylum level. In the colon, we observed a significant glyphosate-associated increase in the relative abundance of Firmicutes (CON, 57.7%; IPA20, 69.4%; IPA200, 66.1%) and a decrease in Bacteroidetes (CON, 32.6%; IPA20, 23.5%). Significant changes were only observed for few genera, e.g., g_0.24 (CON, 7.12%; IPA20, 4.59%; IPA200, 4.00%). In conclusion, exposing weaned piglets to glyphosate-amended feed did not affect gastrointestinal microbial ecology to a degree that was considered actual dysbiosis, e.g., no potential pathogen bloom was observed. IMPORTANCE Glyphosate residues can be found in feed made from genetically modified glyphosate-resistant crops treated with glyphosate or from conventional crops, desiccated with glyphosate before harvest. If these residues affect the gut microbiota to an extent that is unfavorable to livestock health and productivity, the widespread use of glyphosate on feed crops may need to be reconsidered. Few in vivo studies have been conducted to investigate potential impact of glyphosate on the gut microbial ecology and derived health issues of animals, in particular livestock, when exposed to dietary glyphosate residues. The aim of the present study was therefore to investigate potential effects on the gastrointestinal microbial ecology of newly weaned piglets fed glyphosate-amended diets. Piglets did not develop actual gut dysbiosis when fed diets, containing a commercial herbicide formulation or a glyphosate salt at the maximum residue level, defined by the European Union for common feed crops, or at a 10-fold-higher level.

Use of a wiper applicator for the control of the invasive species Eragrostis plana with glyphosate salts

Background: Eragrostis plana is an invasive plant in native grassland areas associated with degradation of the Pampa biome. Selective control of E. plana is difficult due to its similarity to other grassland species. There is a lack of information about E. plana control using selective herbicide application equipment. Objective: The present study aimed to evaluate the efficacy of a pressurize rope wick applicator for controlling the invasive plant E. plana as function of different salts of glyphosate, application volumes, [...]

Volatilization of Dicamba Diglycolamine Salt in Combination with Glyphosate Formulations and Volatility Reducers in Brazil

Dicamba can be included in weed management programs for Brazilian agricultural crops, such as Conyza spp. and Amaranthus spp., and it is essential to implement good management practices that include salts with low volatility levels and appropriate associations to maintain volatility at acceptable levels. The objective of this study was to evaluate the volatilization of dicamba diglycolamine (DGA) salt associated with different glyphosate salts and volatility reducers. Laboratory and field studies were conducted with the application of DGA alone and in mixtures with three glyphosate formulations (potassium salt, ammonium salt, and di-ammonium salt) with and without a volatility reducer. Under laboratory conditions, the sprayed targets (corn straw) were sent to a vapor collection system for subsequent determination of the amount of volatilized dicamba. In the field, the treatments were applied in a tray containing clay soil, and then, these trays were arranged in soybean rows for 48 h under plastic tunnels. The plant injury and the concentrations of the dicamba in the soybean plants at different distances from the tray were determined. The methodologies used in this study were adequate for understanding the volatility of DGA. The volatility of the dicamba DGA salt used was reduced and was managed through the use of volatility reducers and the correct formulation of glyphosate in the mixture. The VR was efficient in reducing the volatility for dicamba alone and DGA in combination with all glyphosate salts. The combination of dicamba DGA salt with glyphosate potassium salt and a volatility reducer was the mixture with the lowest volatility and is the most suitable combination to recommend to farmers.

Individual and integrated methods on tough lovegrass control

ABSTRACT: The current study evaluated the efficiency of mechanical, physical, chemical and cultural methods, used exclusively or integrated, to control tough lovegrass. The experimental design was completely randomized, with 15 treatments and four repetitions. Physical control of tough lovegrass was based on the application of fire, whereas mechanical controls consisted in mowing and harrowing/plowing procedures. The herbicide clethodim and different glyphosate salts were evaluated for chemical control. Glyphosate and soil fertility correction were applied as cultural and integrated methods, in addition to isopropylamine + potassium salts combination, soil fertility correction and implantation of one of the following forage plants: Elephant grass, Pangola grass, Forage peanut and Birdsfoot trefoil. The effect of treatments on the incidence of tough lovegrass and on its botanical composition was evaluated one year after their applications. Isolated control methods, except for glyphosate using, did not present efficient tough lovegrass control. Glyphosate salts could control tough lovegrass plants, but their association with improved soil fertility and Pangola grass implantation was the best strategy to control the invasive plant.

Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays

Cytotoxic effects of the market leading broad-spectrum, synthetic herbicide product Roundup Classic, its active ingredient glyphosate (in a form of its isopropylamine (IPA) salt) and its formulating surfactant polyethoxylated tallowamine (POE-15) were determined on two murine cell lines, a neuroectodermal stem cell-like (NE-4C) and a high alkaline phosphatase activity osteoblastic cell line (MC3T3-E1). Cytotoxicity, genotoxicity, effects on cell viability and cell cycles were examined in five flow cytometry tests, the two former of which were compared by the enzymatic-assay and the alkaline single cell gel electrophoresis (Comet) assay. All of the tests indicated the NE-4C cells being more sensitive, than the MC3T3-E1 cell line to the treatments with the target compounds. Higher sensitivity differences were detected in the viability test by flow cytometry (7–9-fold), than by the MTT assay (1.5–3-fold); in the genotoxicity test by the Comet assay (3.5–403-fold), than by the DNA-damage test (9.3–158-fold); and in the apoptosis test by the Annexin V dead cell kit (1.1–12.7-fold), than by the Caspase 3/7 kit (1–6.5-fold). Cell cycle assays indicated high count of cells (~70%) in the G0/G1 phase for MC3T3-E1 cells, than in NE-4C cell (~40%) after 24 h. The order of the inhibitory potency of the target substances has unequivocally been POE-15 > Roundup Classic > > glyphosate IPA salt.

Rethinking the term “glyphosate effect” through the evaluation of different glyphosate-based herbicide effects over aquatic microbial communities

Glyphosate-based herbicides (GBH) -the most widely used herbicides in agriculture worldwide-are frequently generalized by the name of “glyphosate”. However, GBH encompass a variety of glyphosate salts as active ingredient and different adjuvants, which differ between products. These herbicides reach water bodies and produce diverse impacts over aquatic communities. Yet, the risk assessment assays required for the approval focus mostly on active ingredients. Herein, we compared the effect of five different GBH as well as of monoisopropylamine salt of glyphosate (GIPA) on aquatic microbial communities from natural shallow lakes that were mixed and allowed to evolve in an outdoor pond. We performed an 8-day long assay under indoor control conditions to evaluate the effects of exposure on the structure of nano-plus microphytoplankton (net phytoplankton, with sizes between 2 and 20 μm and >20 μm, respectively) and picoplankton (size ranging between 0.2 and 2 μm) communities through microscopy and flow cytometry, respectively. Significantly different effects were evident on the structure of microbial communities dependent on the GBH, even with herbicides sharing similar active ingredients. Each GBH evoked increases of different magnitude in bacterioplankton abundance. Furthermore, GIPA and a formulation decreased the abundance of a phycocyanin-rich (PC-rich) picocyanobacteria (Pcy) cytometric population and GIPA further altered Pcy composition. Also, two GBH increased net phytoplankton total abundance and, unlike the tested GBH, no apparent effect of GIPA was detected on this community structure. These results demonstrate that GBH effects on aquatic microbial communities should not be summarized as “glyphosate” effects considering that the formulations have effects beyond those exerted by the active ingredients alone. This work intends to alert on the lack of real knowledge regarding the consequences of the variety of GBH on natural aquatic ecosystems. Indeed, the wide use of the term “glyphosate effect” should be thoroughly rethought.

Performance and mineral status of weaning pigs fed diets with different levels of glyphosate and tryptophan

Glyphosate-based herbicides (GBH), like Roundup, are used worldwide for weed control in genetically modified feed crops and pre-harvest desiccation of conventional crops, leaving glyphosate residues in feed made from these sources. Glyphosate possesses herbicidal and antimicrobial properties due to inhibition of the shikimate pathway, involved in aromatic amino acid synthesis in plants and microorganisms, including animal gut bacteria. Furthermore, glyphosate possesses mineral chelating properties. The objective of the present study was to investigate potential effects of dietary glyphosate residues (here glyphosate-amended feed) on livestock, focusing on performance and mineral status of weaning pigs. Two experiments were conducted with pigs from 20 litters (4 females and 4 males per litter), weaned on day 28 of age. In experiment I, 104 pigs from 13 litters were assigned to one of four treatments on the weaning day and fed a standard weaning diet amended with glyphosate as follows: no glyphosate (CON), 20 mg glyphosate/kg as Glyphomax HL (GM20) (Albaugh UK,Ltd., London, UK), 20 mg glyphosate/kg as IPA salt (IPA20) (Monsanto Company, St. Louis, MO, USA) and 200 mg glyphosate/kg as IPA salt (IPA200). The EU maximum residue level (MRL) of glyphosate is 20 mg/kg for a range of common feed ingredients. In experiment II, 56 pigs from seven litters were assigned to one of two treatments on the weaning day and fed a weaning diet similar to that used in experiment I, but without supplementation of crystalline tryptophan. The glyphosate amendments were: no glyphosate (CON-II) and 200 mg glyphosate/kg as IPA salt (IPA200-II). In both experiments, half of the pigs were euthanised on day 9/10 (referred to as day 9) and the other half on day 35 after commencement of the treatments. Individual weight, feed intake and faeces score were registered during the experiment, and small intestine digesta, blood and urine were sampled immediately after euthanising. Analysed levels of glyphosate in the diets were close to the intended levels; only, the glyphosate level in the control diets was not zero, indicating that glyphosate-free diets are difficult to obtain. The concentration of glyphosate in small intestine digesta, serum and urine reflected the treatment levels, e.g. at day 9, serum concentrations were 0.06, 10.9, 11.7 and 68.2 µg/L for CON, GM20, IPA20 and IPA200, respectively. Neither feed intake, growth rate, serum mineral concentrations nor faeces score were significantly affected by level or source (pure glyphosate salt or GBH) of the dietary glyphosate.

The use of a herbicide as a tool to increase livestock consumption of medusahead (Taeniatherum caput-medusae)

AbstractMedusahead [Taeniatherum caput-medusae (L.) Nevski] is an invasive annual grass spreading into rangelands throughout the western United States. We tested cattle (Bos taurus L.) utilization of T. caput-medusae following treatment with glyphosate in two forms of its salt (potassium salt and isopropylamine salt) at three different rates of application; low (236 g ae ha−1), medium (394 g ae ha−1), and high rates (788 g ae ha−1) in eastern Washington. The herbicide was applied on April 26, 2016. A second location, northern Utah, was treated with glyphosate in the form of its isopropylamine salt at the high rate. The herbicide was applied on June 5, 2019. Cattle were allowed to start grazing T. caput-medusae 15 d after glyphosate treatment and had unlimited access to the glyphosate-treated plots for more than 85 d. The greatest utilization of T. caput-medusae occurred at the highest glyphosate application rate (P < 0.05), in Washington, with no difference between forms of glyphosate salt. Cattle also consumed T. caput-medusae at the Utah site (P < 0.05). Glyphosate treatment preserved the water-soluble carbohydrate content of T. caput-medusae at levels greater than the nontreated controls (P < 0.05) at both locations. The glyphosate treatment assisted in the increased utilization of T. caput-medusae by cattle and is a viable option for the reduction of T. caput-medusae while increasing the forage value of the weed.

Glyphosate-resistant soybeans yield in function of glyphosate salts, doses and stadium phenological

Given the importance of soybean to the global economy and as a food source, improving crop management techniques is integral to obtaining higher yields. As such, this study aimed to assess the yield of soybean cultivars as a function of different glyphosate salt formulations, doses and application times. Two field experiments were conducted simultaneously using a randomized block design and 3x4x2 factorial scheme, with three repetitions. Factor A consisted of three glyphosate formulations: isopropylamine salt (Roundup Original®), ammonium salt (Roundup WG®), and potassium salt (Zapp Qi®), factor B four application times: 0, 14, 28, 42 days after planting (DAP), and factor C two doses: the recommended dose of 720 grams of acid equivalent per hectare and twice that (1440 grams of acid equivalent per hectare). There was no difference between the glyphosate salts for the BMX Ativa RR® cultivar, but yields of BMX Apolo RR® submitted to ammonium salt were 28% higher than those recorded for the other salts studied. Glyphosate potassium salt resulted in lower soybean yields. Potassium salt application between 14 and 28 DAP caused greater toxicity in both the soybean cultivars analyzed.

Evaluation of the cytotoxic effects of glyphosate herbicides in human liver, lung, and nerve

Glyphosate-based herbicides are broad-spectrum pesticides widely used in the world, which is considered a highly safe pesticide due to their target specificity, but recently, there has been an ongoing controversy regarding their carcinogenicity and possible side effects of glyphosate on human health. Commercial glyphosate-based herbicides (GBHs) consist of declared active ingredient (glyphosate salts) and a number of formulants such as ethoxylated formulants (4130®, 3780®, and A-178®). The aim of our study is to investigate whether the toxicity of GBHs is related to formulants. The effects of GBHs on human health were studied at the cellular level based on their toxicity to liver, lungs and nerve tissue. The inhibitory toxicity to cell viability by GBHs was examined with cell-based systems using three human cell lines: HepG2, A549, and SH-SY5Y. Data obtained showed that all tested ethoxylated formulants and their mixtures with declared active ingredient glyphosate isopropylamine salt (GP) have significant inhibitory effect on cell proliferation, while the declared active ingredient has no significant toxicity. Our study demonstrates that the toxic effect of GBH is primarily due to the use of formulants. This result suggests that GP is relatively safe and a new approach for the assessment of toxicity should be made.

Glyphosate-based herbicides toxicity on life history parameters of zoophytophagous Podisus nigrispinus (Heteroptera: Pentatomidae)

The increase of agricultural areas with glyphosate-resistant (GR) crops, and use of this herbicide in Brazil, makes necessary to assess its impacts on non-target organisms. The objective was to evaluate the development, reproduction and life table parameters of Podisus nigrispinus (Heteroptera: Pentatomidae) reared on GR-soybean plants treated with glyphosate formulations (Zapp-Qi, Roundup-Transorb-R and Roundup-Original) at the recommended field dose (720g acid equivalent ha−1). Glyphosate formulations had no affect on nymph and adult weight of this predator. Fourth instar stage was shortest with Zapp Qi. Egg-adult period was similar between treatments (26 days) with a survival over 90%. Zapp-Qi and Roundup-Transorb-R (potassium-salt: K-salt) reduced the egg, posture and nymph number per female, and the longevity and oviposition periods of this predator. Podisus nigrispinus net reproductive rate was highest in GR-soybean plants treated with Roundup-Original (isopropylamine-salt: IPA-salt). However, the duration of one generation, intrinsic and finite increase rates, and time to duplicate the population, were similar between treatments. Glyphosate toxicity on P. nigrispinus depends of the glyphosate salt type. IPA-salt was least harmless to this predator. Formulations based on K-salt altered its reproductive parameters, however, the development and population dynamic were not affect. Therefore, these glyphosate formulations are compatible with the predator P. nigrispinus with GR-soybean crop.

Glyphosate Efficacy of Different Salt Formulations and Adjuvant Additives on Various Weeds

In many crops, weeds are managed by herbicides, mainly due to the decrease in crop yields and farmers’ incomes caused by them. In general, chemical control of weeds is considered to be an easy, relatively cheap, and highly effective method. However, not all weeds can be successfully controlled, either because of their natural tolerance or their herbicide resistance. Glyphosate is one of the most widely used herbicides in the world. It can manage effectively a broad spectrum of weeds, and promotes conservation agriculture by significantly reducing conventional plough tillage. Unfortunately, its extensive use has led to the evolution of glyphosate resistance, which has evolved into a major problem for global crop production. Alternative herbicides are, in some cases, available, but they do not usually control certain weeds as efficiently as glyphosate. The transmission of herbicides to the target site is a complex process, and consists of several stages. Each herbicide is affected and can be manipulated by the product formulation for the optimization of its use. Many experiments have confirmed that different glyphosate salts and adjuvant additives are instrumental in the optimization of herbicide absorption and delivery processes. The objective of this paper is to provide a brief overview of these experiments and summarize the literature related to the effect of various glyphosate formulations and adjuvants on weed control. Determining the differences among formulations and adjuvants may lead to the further optimized long-term use of glyphosate.

Effects of glyphosate and the glyphosate based herbicides Roundup Original® and Roundup Transorb® on respiratory morphophysiology of bullfrog tadpoles

Glyphosate-based herbicides are widely used in agriculture and are commonly found in water bodies. Roundup Original® (RO) contains an isopropylamine glyphosate (GLY) salt containing the surfactant POEA, while Roundup Transorb R® (RTR) contains a potassium salt of GLY with unknown surfactants. Both contain different compositions of so-called “inert” ingredients, more toxic than glyphosate. Amphibian tadpoles often experience variations in O2 availability in their aquatic habitats; an ability to tolerate hypoxia can condition their survival and fitness. We evaluated the impacts of sublethal concentrations of GLY (1 mg L−1), RO (1 mg L−1 GLY a.e) and RTR (1 mg L−1 GLY a.e) on metabolic rate (V·O2 – mLO2 Kg1 h−1) of bullfrog tadpoles during normoxia and graded hypoxia, and related this to morphology of their skin, their major site of gas exchange. In control (CT) V·O2 remained unaltered from normoxia until 40 mmHg, indicating a critical O2 tension between 40 and 20 mmHg. GLY significantly reduced V·O2, possibly due to epidermal hypertrophy, which increased O2 diffusion distance to O2 uptake. In contrast, RTR increased V·O2 during hypoxia, indicating an influence of “inert” compounds and surfactants. V·O2 of RO did not differ from CT, suggesting that any increase in V·O2 caused by exposure was antagonized by epidermal hypertrophy. Indeed, all herbicides caused marked alterations in skin morphology, with cell and epithelium wall presenting hyperplasia or hypertrophy and chromatid rupture. In summary, GLY, RO and RTR exert different effects in bullfrog tadpoles, in particular the surfactants and inert compounds appear to influence oxygen uptake.

Studies of Acute and Chronic Toxicity of Commercial Herbicides with Glyphosate against Danio rerio

Glyphosate or N-(phosphonomethyl) glycine is a broad spectrum non-selective systemic herbicide, used to kill weeds, mainly in soybean crops. Nowadays there are many controversies about the intensive use of these herbicides due to the potential environmental impact and the effects on human health. The environmental impacts of commercial Glyphosate formulation Roundup were assessed by evaluation of acute and chronic toxicity of Danio rerio fish. The effects of glyphosate commercial formulations and glyphosate isopropylamine salt solutions were evaluated in different steps. First the lethal doses of Roundup herbicide toward the experimental models were determined. Subsequently the specie was exposed to sublethal concentrations of both, the commercial preparations and pure glyphosate salt in order to evaluate the chronic toxicity. Acute toxicity was assessed by calculating the mortality indexes and chronic toxicity by measuring several biochemical parameters such as the activity of marker enzymes like aspartate aminotransferase (AST), alanine aminotransferase (ALT) and acetylcholinesterase (AchE). The registers of histological alterations in liver tissue sections were also considered in this study. Commercial herbicides produce mortality of D. rerio and sublethal doses of these herbicides and the salt of the pure compound produce effects of chronic toxicity at the liver and muscle level; such are enough causes to limit the potential survival of these organisms in the medium.

A multicenter retrospective survey of poisoning after ingestion of herbicides containing glyphosate potassium salt or other glyphosate salts in Japan

ABSTRACTIntroduction: A multicenter retrospective survey of patients poisoned by herbicides containing glyphosate salts in Japan was conducted to identify differences in symptoms and outcome of poisoning. Methods: Participants were patients who were transported to emergency facilities between October 2006 and March 2014 after consuming herbicides containing glyphosate potassium salt (GlyK+) (the K-group) or other glyphosate salts (the O-group). Questionnaires were mailed to 38 emergency facilities that agreed to participate in the study. Results: Serum potassium levels upon arrival were significantly higher (p < 0.01), and abnormal electrocardiogram findings were significantly more common (p < 0.01) in the K-group (n = 55) than in the O-group (n = 62). Conversely, acute lung injury (ALI) including acute respiratory distress syndrome (ARDS) (p = 0.05) and liver injury (LI) (p < 0.01) were significantly more common during hospitalization in the O-group, although no significant differences in the duration of hospital stay (p = 0.92) or outcomes (p = 0.95) were observed between the two groups. Discussion and conclusion: The ingestion of products containing glyphosate isopropylamine or ammonium salts, and polyoxyethyleneamine (POEA) as a surfactant, can cause severe organ injury. Physicians should note that the ingestion of products containing glyphosate potassium salt and surfactants other than POEA can cause hyperkalemia, potentially leading to fatal arrhythmias or cardiac arrest.

Effect of different glyphosate salts on phosphodiesterase and phosphotriesterase activities in soil with reference to ecological importance of soil pollution. A laboratory experiment

Abstract The aim of this study is to determine the effect of the two glyphosate salts: isopropylamine and potassium (contained in preparations Roundup) on the activity of phosphodiesterase and phosphotriesterase in the soil. The experiment was carried out in the laboratory conditions on two soil types: loamy sand (Corg 8.70 g·kg−1, pHKCl 6.39) and sandy loam (Corg 10.90 g·kg−1, pHKCl 6.81). Two glyphosate salts (isopropylamine and potassium) in dosage of 0, 1 and 100 mg·kg−1 were applicate into soils. Phosphodiesterase and phospotriesterase activities in soils were determined spectrophotometrically on days 1, 7, 14, 28, 56 and 112. The obtained results were converted with respect to the enzyme activities in the control soil (assuming it to be 100%) and given as percent of inhibition. The results were shown as environmental danger zones graphs. Obtained results showed that glyphosate salts application has primarily resulted in inhibition of phosphodiesterase activity, and stimulation of phosphotriesterase activity in soils. Soil type and kind of glyphosate salt affect the interaction of herbicide with phosphodiesterase and phosphotriesterase activities. The observed changes in the phosphodiesterase and phosphotriesterase activities caused by the addition of both glyphosate salts were located on environmental danger zones graphs in the range of negligible or acceptable values, which may indicate a low impact of herbicide on measured enzymes.

Hygroscopic salts support the stomatal penetration of glyphosate and influence its biological efficacy

The objective of this study was to better understand the role of chaotropic and kosmotropic salts in stomatal uptake, a process that occurs in parallel with cuticular penetration and affects the bioefficacy of glyphosate. In Trial 1, salt solutions of kosmotropic (NH4)2SO4 and chaotropic NaClO3, with or without the organosilicone surfactant, Break‐Thru® S233 (BT), and with or without glyphosate, were prepared. In Trial 2, sodium salts with a kosmotropic‐to‐chaotropic nature (Na2SO4, NaCl, NaNO3 or NaClO3) and BT were prepared with or without glyphosate. At very high concentrations, the salts of a kosmotropic nature induced a slight decrease in the surface tension, whereas those of a chaotropic nature triggered a significant decrease in the surface tension. Solution droplets were deposited onto the leaves of Viola arvensis and Chenopodium album. The dried deposits were analyzed with an environmental scanning electron microscope. The salts formed structures linking the exterior to the interior of the stomata in the crystalline ([NH4]2SO4) or amorphous (NaClO3) form. However, the low surface tension alone did not appear to be the unique driving force for stomatal uptake. With glyphosate, amorphous globular structures of glyphosate salt were formed in the Na2SO4 (kosmotropic) solution. Differently, the salts of a rather chaotropic nature (NaNO3 or NaClO3) formed a colloidal matrix. In general, salt residues were observed over the periclinal cell walls and inside the stomatal chamber. Neither (NH4)2SO4 nor Na2SO4 had a significant impact on glyphosate bioefficacy. However, the chaotropic salt, NaNO3, enhanced the bioefficacy of glyphosate the most, with a performance that was comparable to that of the solution glyphosate + organosilicone surfactant.

Application of Ferric Chloride for Removal of Glyphosate: Modeling of Axial and Radial Flow Impellers Using Artificial Neural Networks

AbstractThis paper studied the removal of the herbicide Glyphosate in aqueous solution using ions capable of forming an insoluble complex Glyphosate salt (ferric chloride). A maximum Glyphosate removal of 67.4% was achieved using 300 mg/L of ferric chloride. The precipitation of the insoluble Glyphosate salt was affected by rapid mixing velocity, rapid mixing time, dosage of flocculant, and types of impellers. An artificial neural networks (ANN) model was used to predict the removal of Glyphosate. The results showed good agreement over the range of experimental and predicted data. Increasing the velocity gradient increased the Glyphosate removal. The Glyphosate removal decreased with further increase in shear stress. At higher flocculant dosage, the effect of impeller shear is less as the flocs are stronger. The results showed that the effect of impeller type was highly dependent on the rapid mixing velocity and rapid mixing time.