Aim: Wound location infections are among the first three of hospital-acquired infections. During the treatment of wound infections, culture and antibiogram sensitivities will increase the success of the treatment and prevent the use of the wrong antibiotics and prevent the development of resistance in the bacteria. In our study, between the years 2016-2018 outpatient and inpatient wound distribution of microorganisms isolated from samples from patients we determined the rate and antibiotic susceptibilities were meant to be guiding the empirical treatment options. Material and Methods: A total of 315 wound sites were examined retrospectively in our microbiology laboratory. Descriptive microorganisms were determined at species level and antimicrobial susceptibility tests were investigated. Results: Of the isolated microorganisms, 46.4% (n=146) were Gram positive cocci, 53% (n=167) were Gram negative bacteria and 0.6% (n=2) were fungi ( Candida albicans ). S. aureus was the second most frequent coagulase negative staphylococcus (16%), E. coli was the third most common (15%) (p<0.001). In the case of Gram positive bacteria, 16.7% of S. aureus strains were resistant to methicillin. There was no glycopeptide resistance in Gram positive bacteria including enterococci. The ESBL ratio in E. coli and K. pneumoniae strains (43.8%, 48.5%) was higher than the other Gram negative bacteria (p=0.493). The highest antibiotic resistance rate was found to be against ampicillin with 70.8% and to imipenem with a minimum resistance rate of 2%. Conclusion: In our study, S. aureus and E. coli , which are frequent agents of wound infections, were considered to be quite high in patients with gastrointestinal carcinoma. Therefore, it was thought that the antibiotic susceptibility of these agents should be considered in empirical treatment.