Glycine Methyl Ester Research Articles

Glycinamide Facilitates Nanocomplex Formation and Functions Synergistically with Bone Morphogenetic Protein 2 to Promote Osteoblast Differentiation In Vitro and Bone Regeneration in a Mouse Calvarial Defect Model.

This study aimed to identify glycine analogs conducive to the formation of cell-absorbable nanocomplexes, enhancing collagen synthesis and subsequent osteogenesis in combination with BMP2 for improved bone regeneration. Glycine and its derivatives were assessed for their effects on osteogenic differentiation in MC3T3-E1 cells and human bone marrow mesenchymal stem cells (BMSCs) under osteogenic conditions or with BMP2. Osteogenic differentiation was assessed through alkaline phosphatase staining and real-time quantitative polymerase chain reaction (RT-qPCR). Nanocomplex formation was examined via scanning electron microscopy, circular dichroism, and ultraviolet-visible spectroscopy. In vivo osteogenic effects were validated using a mouse calvarial defect model, and bone regeneration was evaluated through micro-computed tomography and histomorphometric analysis. Glycine, glycine methyl ester, and glycinamide significantly enhanced collagen synthesis and ALP activity in conjunction with an osteogenic medium (OSM). GA emerged as the most effective inducer of osteoblast differentiation marker genes. Combining GA with BMP2 synergistically stimulated ALP activity and the expression of osteoblast markers in both cell lines. GA readily formed nanocomplexes, facilitating cellular uptake through strong electrostatic interactions. In an in vivo calvarial defect mouse model, the GA and BMP2 combination demonstrated enhanced bone volume, bone volume/tissue volume ratio, trabecular numbers, and mature bone formation compared to other combinations. GA and BMP2 synergistically promoted in vitro osteoblast differentiation and in vivo bone regeneration through nanocomplex formation. This combination holds therapeutic promise for individuals with bone defects, showcasing its potential for clinical intervention.

Hyaluronic acid conjugates of glycine peptides and L-tryptophan

This work reports about the conjugation of glycine C-terminal ethyl and methyl ester peptides and L-tryptophan methyl ester with sodium hyaluronate in aqueous solutions using the peptide coupling agent DMTMM (or short DMT, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride). Detailed infrared (IR) absorbance and 1H and 13C (2D) NMR studies (heteronuclear multi-bond correlation spectroscopy, HMBC) confirmed covalent and regioselective amide bonds with the D-glucuronate, but also proves the presence of DMT traces in all conjugates. The ethyl ester`s methyl protons on the peptides` C-terminal could be used to quantify the degree of substitution of the peptide on the hyaluronate scaffold by NMR. The ester group also proved stable during conjugation and work-up, and could in some cases be selectively cleaved in water whilst leaving the amide bond intact as shown by potentiometric charge titration, NMR and IR. The conjugates did not influence the capability of human umbilical vein endothelial cells (HUVECs) to reduce MTS (5-[3-(carboxymethoxy)phenyl]-3-(4,5-dimethyl-2-thiazolyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) to a formazan dye, which points towards a low cytotoxicity for the obtained products. The conjugation method and products could be tested for tissue engineering gels or drug delivery purposes with alternative, biologically active peptides.

Synthesis of pyrrolo[2,3-d]pyridazines and pyrrolo[2,3-c]pyridines (6-azaindoles). Experimental and theoretical study.

A new synthetic route towards 6-azaindoles (pyrrolo[2,3-c]pyridines) and pyrrolo[2,3-d]pyridazines starting from 4-aroyl pyrroles is described. This overall protocol involves: (i) the Vilsmeier-Haack reaction to obtain pyrrolo-2,3-dicarbonyles and (ii) condensation with hydrazines or glycine methyl ester. The reaction mechanism between pyrrolo-2,3-dicarbonyl with phenyl hydrazine and glycine methyl ester has been modelled using DFT calculations to prove the formation of one from two possible isomers of condensation.

New synthesis of β-aryl-GABA drugs

A new synthetic method for the preparation of β-aryl-GABA drugs is reported. The key step for the construction of α,β-unsaturated-β-aryl-γ-lactam derivatives relies on a novel cascade radical reaction. An N-propargyl aryl amide was rapidly constructed starting from an aryl carboxylic acid and propargyl amine. Protection of the amide N–H with a Boc group, followed by a cascade radical aryl migration reaction, produced the key intermediate, an unsaturated β-aryl-γ-lactam. Further catalytic hydrogenation and deprotection gave rolipram. Meanwhile, coupling of the glycine methyl ester and propargyl bromide, followed by benzoylation gave the radical precursor. The radical reaction resulted in the production of the unsaturated β-aryl-γ-lactam with the glycine moiety. After aminolysis reactions, phenylpiracetam and phenylpiracetam hydrazide were produced, respectively. This synthetic protocol provides a general and simple approach for preparing racemates or chiral forms of β-aryl-GABA drugs and analogues.

Growth, structural, spectroscopic, DFT calculations, insilico biological analysis of glycine methyl ester hydrochloride (GMEHCl) against human estrogen receptor

On Glycine Methyl Ester Hydrochloride (GMEHCl), X-ray crystallographic (Lattice parameter), FT-IR, FT-Raman, DFT, HOMO-LUMO, MEP, ADMET, and molecular docking investigations are studied. The small molecule (GMEHCl) docking analysis with the target protein revealed that this is a good molecule that docks well with a target connected to the Human estrogen receptor macromolecule. The improvement of electrostatic interactions between the protein and the ligand is made possible by molecular electrostatic potential (MEP). DFT-B3LYP calculations using the 6–31++G (d,p) basis set are used to determine the optimized geometry of the GMEHCl molecule, and the computed vibrational frequencies are assessed by comparing them to experimental results. Using a 2D fingerprint plot and Hirshfeld Surfaces, the molecular surface and hydrogen bonding interactions in the GMEHCl crystal structure were discovered and investigated. The parameters of absorption, distribution, metabolism, and excretion (ADME) were measured using the internet server preADMET.

Synthesis, crystal structure, and intrinsic reactivity descriptors of coordination complexes of [(cis-PdCl2·L-proline) L-proline] and [trans-PdCl2·(glycine-OMe)2

This work describes the synthesis and structural characterization of two palladium (II) complexes elucidated by infrared spectroscopy, dispersive energy analysis (EDS) with Scanning Electron Microscopy (SEM) and X-ray single-crystal diffraction analysis. The first chiral complex is derivatized from l-proline amino acid, obtaining [Cis-PdCl2·L-proline(L-proline)] (1), in which the structure does not have symmetry elements and crystallized in a triclinic crystal system on a non-centrosymmetric space group P1. The comparison with the structural complex of [trans-PdCl2·(glycine-OMe)2] (3) becomes necessary due to the anticancer activity of its analog, cis-platinum, and the catalytic activity caused by Pd as a metallic center. Complex 1 is therefore made up of a free proline molecule linked by a single hydrogen bond to a PdCl2 molecule forming a 5-member chelate ring of the COO···Pd···NH type with another proline molecule and displaying distorted angles and bond sizes to the square planar geometry of the palladium (Pd) center. Ring formation generates a new stereogenic center chemically correlated with the induction caused by the absolute configuration S of the initial amino acid. The crystal results strongly indicate the confirmed configuration by the Flack parameter and its crystal packing stabilization by an intermolecular H-bond network, highlighting as a secondary mimicking structure the β sheets formed by hydrogen bonding interaction of the N—H···Cl type responsible for the disorder moldered on pyrrolidine ring.Furthermore, the Pd(II) complex obtained by the glycine was [trans-PdCl2·(glycine-OMe)2] (3). Regarding its chemical structure, this complex is constituted by the union of two glycine methyl ester molecules binding to a Pd in a trans disposition concerning the almost perfect square plane, which also contains a C2 axis of rotation. Complex 3 crystallized in a monoclinic system and centrosymmetric space group P21/c, the packing is favored by four strong hydrogen bond type interactions presenting helices with 90° rotations between each layer.Owing to their great interest and relevance in Pd—N amino acid in catalytic and medicinal chemistry, the electronic and global reactivity properties of complexes 1 and 3 were further explored by computational methods in the framework of Density Functional Theory, finding 1 as the most reactive complex due to the narrower E-LUMO/E-HOMO energy gap, having this band gap in agreement with the lowest hardness value compared to those shown for 3, in which band gap and hardness are higher with a tendency to kinetic stability and controlled reactivity.

Acetyl group for proper protection of β-sugar-amino acids used in SPPS.

The synthesis of D-glucosamine-1-carboxylic acid based β-sugar amino acids (β-SAAs) is typically performed in nine consecutive steps via an inefficient OAc → Br → CN conversion protocol with low overall yield. Here, we present the improved and more efficient synthesis of both Fmoc-GlcAPC-OH and Fmoc-GlcAPC(Ac)-OH, β-SAAs consisting of only 4-5 synthetic steps. Their active ester and amide bond formation with glycine methyl ester (H-Gly-OMe) was completed and monitored by 1H NMR. The stability of the pyranoid OHs protecting the acetyl groups was investigated under three different Fmoc cleavage conditions and was found to be satisfactory even at high piperidine concentration (e.g. 40%). We designed a SPPS protocol using Fmoc-GlcAPC(Ac)-OH to produce model peptides Gly-β-SAA-Gly as well as Gly-β-SAA-β-SAA-Gly with high coupling efficiency. The products were deacetylated using the Zemplén method, which allows the hydrophilicity of a building block and/or chimera to be fine-tuned, even after the polypeptide chain has already been synthesized.

Glycine methyl ester based Schiff base bearing long alkoxy arm and its zinc(II) and copper(II) complexes: Synthesis, mesomorphism, photoluminescence, CT-DNA interaction and DFT study

A new photoluminescent mesogenic glycine methyl ester based Schiff base ligand and its zinc(II) and copper(II) complexes have been synthesised. The compounds were characterized by elemental analyses, Fourier transform infrared spectroscopy (FT-IR), 1H, 13C nuclear magnetic resonance (NMR), ultraviolet–visible spectroscopy (UV–Vis), ESI-MS, MALDI-TOF, EDX and thermogravimetric analysis (TGA). The polarizing optical microscopy (POM) and differential scanning calorimetry (DSC) study revealed that the ligand and zinc(II) complex possess SmecticA mesophase on heating but copper(II) complex is non-mesogenic. The optical absorption and fluorescence spectroscopy studies of zinc(II) complex indicate the existence of aggregation/ de-aggregation species in the presence of non-coordinating and coordinating solvents. The Density Functional Theory (DFT) study was carried out using LanL2DZ and 6–31 G (d, p) basis set with meta-hybrid Truhlar's functional M06–2X to obtain a stable, optimized structure, HOMO-LUMO energy gap and chemical hardness. As designed, the combination of both the –CN- and -COO- linkages plays significant role to influence the mesomorphic behaviour as well as biogenic properties of the compounds. The in vitro interactions of the compounds with CT-DNA has been investigated using UV–Vis and fluorescence spectroscopy. To the best of our knowledge, this is one of the few report on DNA binding studies of mesogenic amino acid Schiff base, which is having binding constant in the same range with the gold standard compound, ethidium bromide.

19 Molecular Docking Analysis of Volatile Compounds from Fraction of Eichhornia crassipes Herbs Ethanol Extract As α-Glucosidase Inhibitor

Diabetes mellitus is a degenerative disease characterized by an increase in blood sugar (hyperglycemia).Inhibiting it will prevent the uptake of glucose in the plasma. One of the plants that can be usedas antidiabetic is E. crassipes herbs. This research aims to know the interaction between volatilechemical compounds from ethyl acetate fraction and ethanol fraction of ethanol extract of E. crassipesherbs with the α-glucosidase enzyme. This study used ethanol extract of E. crassipes herbs with avariation of ethyl acetate fraction and ethanol fraction then analyzed the fraction using GC-MS (GasChromatography-Mass Spectroscopy). The ethyl acetate fraction contained 2-pyridine-propanoicacid, alpha.-methyl-.beta.-oxo-,Ethyl ester;1-propanol-1-di; carbamic acid, methyl ester; acetic acid,aminooxy-, hydrazide; (2R) - [2-2H2] glycine methyl ester hydrochloride. The ethanol fractioncontained semicarbazide hydrochloride; carbonic dihydrazide; hydroxy-acetaldehyde; 1-propanol-1-di; acetic acid, and hydroxyl. The result of docking with the α-glucosidase enzyme is 2-pyridinepropanoicacid, alpha.-methyl-.beta.-oxo-, the Ethyl ester of the ethyl acetate fraction having ΔG is-4.14 Kcal/mol. Semicarbazide hydrochloride from the ethanol fraction has ΔG of -3.17 Kcal/mol.Keywords: Diabetes mellitus, docking molecular, (Eichhornia crassipes (Mart.) Solms Herbs, ethylacetate fraction, α-glucosidase enzyme.

Synthesis of dehydroabietane-derived sulfonamides with fragments of L-amino acid esters and hydrazides

Ethyl ester of 12-chlorosulfodehydroabietic acid, when reacted with the methyl esters of glycine, methionine, leucine, glutamic acid, tyrosine, proline, histidine, for the first time yielded the corresponding sulfonamides, the ester group (-COOMe) of which was selectively transferred to the hydrazide (-COONHNH2) without affecting the ethyl group bounded with the dehydroabietic acid fragment. In the case of glutamic acid, the corresponding dihydrazides were obtained. The reaction of 12-chlorosulfodehydroabietic acid ethyl ester with cystine dimethyl ester led to the selective formation of bis-sulfonamide, while carrying out the same reaction in an acetone-contained solution was accompanied by the cleavage of the disulfide bond of the cystine fragment to give thioketal. The obtained bis-sulfonamide and thioketal were oxidized with chlorine dioxide to afford in both cases sulfochloride.

Synthesis and characterization of functionalized modified PVC-chitosan as antimicrobial polymeric biomaterial

Amino acetic acid modified poly(vinyl chloride), MPVC, was obtained by chemical modification of PVC using glycine methyl ester. MPVC was used as a precursor to prepare some functionalized MPVC conjugates to be used in biomedical applications. MPVC-Cs was prepared by the chemical reaction of MPVC with chitosan as a natural polymer in absence and presence of epichlorohydrin (Ech) as a crosslinking agent. Further chemical modification was performed by the reaction of MPVC with Cs and salicylic acid in the absence in presence of Ech via one-pot reaction. The chemical structure of the formed MPVC, MPVC-Cs, MPVC-Cs/POH, MPVC-Cs/SA and MPVC-Cs/POH/SA was confirmed by the FTIR spectroscopic analysis, scanning electron microscopy, and thermogravimetric analysis (TGA). The antibacterial activity of the prepared MPVC and its conjugates was investigated against two Gram +ve bacteria (Staphylococcus aurous and Listeria monocytogenes) and (Escherichia coli and Salmonella typhi) as Gram −ve bacteria in addition to the Catondida albicans as yeast. Minimum inhibition concentration (MIC) was also determined for the prepared materials.

Multicomponent Molecular Systems Based on Porphyrins, 1,3,5-Triazine and Carboranes: Synthesis and Characterization.

2,4,6-Trichloro-1,3,5-triazine (cyanuric chloride) is an excellent coupling reagent for the preparation of highly structured multifunctional molecules. Three component systems based on porphyrin, cyanuric chloride and carborane clusters were prepared by a one-pot stepwise amination of cyanuric chloride with 5-(4-aminophenyl)-10,15,20-triphenylporphyrin, followed by replacement of the remaining chlorine atoms with carborane S- or N-nucleophiles. Some variants of 1,3,5-triazine derivatives containing porphyrin, carborane and residues of biologically active compounds such as maleimide, glycine methyl ester as well as thioglycolic acid, mercaptoethanol and hexafluoroisopropanol were also prepared. A careful control of the reaction temperature during the substitution reactions will allow the synthesis of desired compounds in a good to high yields. The structures of synthesized compounds were determined with UV-vis, IR, 1H NMR, 11B NMR, MALDI-TOF or LC-MS spectroscopic data. The dark and photocytotoxicity as well as intracellular localization and photoinduced cell death for compounds 8, 9, 17, 18 and 24 were evaluated.

Combination of Glycinamide and Ascorbic Acid Synergistically Promotes Collagen Production and Wound Healing in Human Dermal Fibroblasts.

The purpose of this study is to present a novel strategy to enhance collagen production in cells. To identify amino acid analogs with excellent collagen production-enhancing effects, human dermal fibroblasts (HDFs) were treated with 20 kinds of amidated amino acids and 20 kinds of free amino acids, individually at 1 mM. The results showed that glycinamide enhanced collagen production (secreted collagen level) most effectively. Glycine also enhanced collagen production to a lesser degree. However, other glycine derivatives, such as N-acetyl glycine, N-acetyl glycinamide, glycine methyl ester, glycine ethyl ester, and glycyl glycine, did not show such effects. Glycinamide increased type I and III collagen protein levels without affecting COL1A1 and COL3A1 mRNA levels, whereas transforming growth factor-β1 (TGF-β1, 10 ng mL−1) increased both mRNA and protein levels of collagens. Ascorbic acid (AA, 1 mM) increased COL1A1 and COL3A1 mRNA and collagen I protein levels. Unlike TGF-β1, AA and glycinamide did not increase the protein level of α-smooth muscle actin, a marker of differentiation of fibroblasts into myofibroblasts. The combination of AA and glycinamide synergistically enhanced collagen production and wound closure in HDFs to a level similar to that in cells treated with TGF-β1. AA derivatives, such as magnesium ascorbyl 3-phosphate (MAP), 3-O-ethyl ascorbic acid, ascorbyl 2-O-glucoside, and ascorbyl tetraisopalmitate, enhanced collagen production, and the mRNA and protein levels of collagens at 1 mM, and their effects were further enhanced when co-treated with glycinamide. Among AA derivatives, MAP had a similar effect to AA in enhancing wound closure, and its effect was further enhanced by glycinamide. Other AA derivatives had different effects on wound closure. This study provides a new strategy to enhance cell collagen production and wound healing using glycinamide in combination with AA.

Rhodium(I) carbonyl complexes containing amino acid ester ligands: synthesis, reactivity and DFT studies

Bridge-splitting reaction of the dimer [Rh(CO)2Cl]2 with two molar equivalents of amino acid ester ligands yield the rhodium(I) dicarbonyl complex [Rh(CO)2ClL] (1a-g) {L = glycine methylester (a), alanine methylester (b), alanine ethylester (c), alanine t-butylester (d), phenylalanine methylester (e), phenylalanine ethylester (f), valine methylester (g)}. Complexes 1 undergo oxidative addition (OA) with CH3I to give the five-coordinate Rh(III) complexes of the type [Rh(CO)(COCH3)IClL] (2a–g). The complexes have been characterized by elemental analysis, IR and 1H NMR spectroscopy. Theoretical calculations reveal that the synthesized complexes 1 are very prone to undergo oxidative addition with CH3I with a low activation barrier.

Syntheses, Structures and Cytotoxicity of Cu(II) Complexes with α‐Amino acid derived Benzamidines

AbstractN‐(diethylthiocarbamoyl)benzimidoyl chloride reacts with methyl esters of glycine, L‐alanine, L‐valine under formation of pro‐ligands 1 which undergo de‐esterification in reactions with Cu(CH3COO)2 and finally form complexes 2 with the compositions of [{Cu(L‐gly)}4] (2 a), [{Cu(L‐ala)}∞] (2 b) and [{Cu(L‐val)}∞] (2 c). In all complexes, Cu(II) ion is coordinated by a dianion {L2−} via its (O,N,S) donor set and a bridging carboxylate O atom, and thus, adopts a distorted square‐planar coordination sphere. Treatment of complexes 2 with equimolar amounts of o‐phenanthroline results in the formation of square pyramidal ternary complexes with the compositions of [Cu(L)phen] (L=L‐gly, L‐ala, L‐val) (3). Antiproliferative effects of the compounds on MCF‐7 and Hep‐G2 cancer cells were studied. Generally, the ternary complexes 3 are more active than the complexes 2 and the pro‐ligands 1. In each series of compounds, the derivatives of L‐valine show higher cytotoxicity than those of L‐alanine and glycine.

Targeted delivery of emamectin benzoate by functionalized polysuccinimide nanoparticles for the flowering cabbage and controlling Plutella xylostella.

Plutella xylostella, one of the most destructive and cosmopolitan pests of cruciferous crops, is especially harmful to the young tissues of the flowering cabbage (Brassica campestris L.). Although emamectin benzoate (EB) has high insecticidal activity against P.xylostella, one major reason of low utilization for EB is the lack of internal transport in the young plants. In this study, four kinds of functional EB/polysuccinimide (PSI) with glycine methylester nanoparticles (EB@PGA NPs) were prepared. The obtained EB@PGA NPs could effectively protect EB from photolysis, and the degradation rate of EB@PGA NPs was <30% in 24 h. Simulating the intestinal pH=9 of P.xylostella, the highest cumulative release rate of EB@PGA NPs could reach 89.61% in 24 h. Furthermore, EB@PGA NPs could delivery EB into the young tissues of the flowering cabbage through the nanocarrier, and the highest transport efficiency of EB@PGA25 reached 1.437%. The bioactivity of EB@PGA25 against P.xylostella larvae (LC50 =0.34μg mL-1 ) was 1.6-fold higher than that of EB (LC50 =0.53μg mL-1 ). EB@PGA could easily become 'internalized' into the intestinal wall of P.xylostella, thus increasing the penetration of the drug and enhancing the insecticidal activity. The accurate delivery of insecticides by PGA nanocarriers into young tissues of plants could be a promising new method for the efficient management of field pests and diseases. © 2021 Society of Chemical Industry.

A high-efficient nano pesticide-fertilizer combination fabricated by amino acid-modified cellulose based carriers.

As the global population grows, large quantities of agrochemicals are used to secure food demand and there is an urgent need to develop efficient and environmentally friendly pesticides and fertilizers. Compared with traditional pesticides, nanopesticide formulations can achieve better foliar wettability, chemical stability, and water dispersibility without or with the minimal use of organic solvents, in line with the development of sustainable agriculture. In this research, glycidyl methacrylate (GMA) was used as an intermediate and glycine methyl ester (GLY) was used as an organic nitrogen source to modify carboxymethyl cellulose (CMC) to synthesize a pesticide nanocarrier CMC-PGMA-GLY. A nanopesticide EB@CMC-PGMA-GLY (EB@CPG) was formed by the hydrophobic and electrostatic interactions of emamectin benzoate (EB), which had good water dispersion. The good affinity of the composite towards leaves led to the effective moistening of the leaf surface by the pesticide and through the pH control pesticide release. Biological experiments show that nanopesticides can maintain high insecticidal activity and have no toxicity to seed germination. The results of pot experiment showed that the nanocarriers could be used as an organic nitrogen fertilizer to increase the fresh weight of plants by 39.77%. This environmentally friendly pesticide carrier can be used as a fertilizer to provide sufficient nutrients for crops, opening a new method for the development of sustainable agriculture. © 2021 Society of Chemical Industry.

Water-based carbodiimide mediated synthesis of polysaccharide-amino acid conjugates: Deprotection, charge and structural analysis

We report here a one-step aqueous method for the synthesis of isolated and purified polysaccharide-amino acid conjugates. Two different types of amino acid esters: glycine methyl ester and L-tryptophan methyl ester, as model compounds for peptides, were conjugated to the polysaccharide carboxymethylcellulose (CMC) in water using carbodiimide at ambient conditions. Detailed and systematic pH-dependent charge titration and spectroscopy (infrared, nuclear magnetic resonance: 1H, 13C- DEPT 135, 1H- 13C HMBC/HSQC correlation), UV–vis, elemental and ninhydrin analysis provided solid and direct evidence for the successful conjugation of the amino acid esters to the CMC backbone via an amide bond. As the concentration of amino acid esters increased, a conjugation efficiency of 20–80% was achieved. Activated charcoal aided base-catalyzed deprotection of the methyl esters improved the solubility of the conjugates in water. The approach proposed in this work should have the potential to tailor the backbone of polysaccharides containing di- or tri-peptides.

Application of allylic amine formation from aziridine-2-ol under Appel reaction condition: Synthesis of N-(tert-butoxycarbonyl)-D-vinyl glycine methyl ester

PPh3/I2/imidazolde mediated allyl amine formation from aziridine-2-alcohol was explored for the synthesis of N-(tert-butoxycarbonyl)-D-vinyl glycine methyl ester.

Kinetics, mechanism and DFT calculations on base hydrolysis of -amino acid esters catalyzed by [Pd(TMPDA)(H2O)2]2+

Pd(TMPDA)Cl2 (TMPDA = N,N,N`,N`-tetramethyl-1,3-propanediamine) was synthesized and characterized by elemental analysis. [Pd(TMPDA)(H2O)2]2+ reacts with amino acid esters (L) to form mixed ligand [Pd(TMPDA)L]2+ complexes. The kinetics of base hydrolysis of [Pd(TMPDA)L]2+ was studied by pH-stat technique and the corresponding rate constants are reported. The coordinated glycine methyl ester is efficiently hydrolyzed, whereas the coordinated methionine methyl ester is hydrolysed with a much lower catalytic activity. The catalytic influence is depended on the mode of coordination of the ester to the palladium complex. Probable mechanisms for these reactions are considered. Activation parameters for the hydrolysis of the coordinated glycine methyl ester were determined experimentally. DFT calculations (B3LYP/LANL2DZ) were applied to determine the possible mechanism of the base hydrolysis of the amino acid esters. The calculations are discussed in reference to the reported experimental data.