Protein Content Gluten Research Articles

Dynamic Study on Water State and Water Migration during Gluten-Starch Model Dough Development under Different Gluten Protein Contents.

Mixing is crucial for dough quality. The gluten content influences water migration in dough development and properties, leading to quality changes in dough-based products. Understanding how the gluten protein content influences water migration during dough development is necessary for dough processing. A compound flour with different gluten protein contents (GPCs, 10-26%, w/w) was used to study the dough farinograph parameters and water migration during dough development. According to the farinograph test of the gluten-starch model dough, the GPC increases the water absorption and the strength of the dough. Water migration was determined via low-field nuclear magnetic resonance (LF-NMR). With the increase in GPC, the gluten protein increases the binding ability of strongly bound water and promotes the transformation of weakly bound water. However, inappropriate GPC (10% and 26%, w/w) results in the release of free water, which is caused by damage to the gluten network according to the microstructure result. Moreover, the changes in proteins' secondary structures are related to the migration of weakly bound water. Therefore, weakly bound water plays an important role in dough development. Overall, these results provide a theoretical basis for the optimization of dough processing.

QUALITY CONTROL METHODS OF SHORTCRUST BISCUITS USING HIGHLY NON-TRADITIONAL RAW MATERIALS

Butter cookies refer to products obtained on the basis of visco-plastic dough, which is characterized by a significant amount of fat components, sugar, and the use of wheat flour with an average gluten content. Such a formulation makes the finished product high-calorie and low biological value. The results of research aimed at enriching butter cookies by replacing wheat flour with amaranth flour and a mixture of sprouted cereals with simultaneous regulation of its sugar content by introducing polydextrose are presented in the article. Based on theoretical and experimental studies, it was established that the addition of amaranth flour contributed to the development of springy deformations in the dough and reduced the development of plastic deformations, led to friability, which is associated with a decrease in the content of gluten proteins, the heterogeneity of starch grains, including a significant increase in the amylopectin fraction of starch. It was found that maximum possible replacement was 50% of wheat flour with amaranth flour, which did not impair the taste and aroma of the finished product, but coused dark color both of the dough and the finished product. More amount of replacement of wheat flour with a crushed mixture of sprouted cereals deepened the negative effect on the structural and mechanical properties of the dough, namely, the general deformation of the samples increased, the relative plasticity decreased, and the relative springiness increased. Such regularities are caused by the introduction of a significant amount of dietary fibers into the mixture, as well as low molecular weight water-soluble compounds accumulated due to hydrolytic processes during germination. In the finished products, the baking index increased, there was also an increase in friability. The possibility of adding polydextrose to replace 50% of powdered sugar was experimentally confirmed to improve the structural and mechanical characteristics of dough based on a mixture of amaranth flour, germinated cereal and wheat flour, but the effect on the plastic characteristics of such a replacement was insignificant. In order to achieve the necessary quality indicators, it was proposed to introduce the cellulolytic enzyme preparation Alhamalt HCF from the manufacturer "Stern Ingredients" LLC. It was established that significant changes occurred in the consistency of the dough that was left to rest at a temperature of 35°C, it acquired the plasticity necessary for forming, did not tear, the dough blanks were not characterized by deformation of the seams, and the finished cookies had a smooth, uncracked surface. In the technological scheme for the production of butter cookies with a high content of non-traditional raw materials, it was proposed to introduce a dough resting stage with a temperature of 35 °C, which will allow the enzymatic hydrolysis of cellulose and its derivatives under the action of the introduced enzyme preparation, increase the accumulation of watersoluble sugars, which will lead to the plasticization of the semi-finished product

Effect of gluten protein levels on physicochemical and fermentation properties of corn dough

SummaryIn this experiment, the effects of gluten protein levels of 6.83% (low gluten), 10.24 (medium gluten), and 13.65% (high gluten), on the physicochemical and fermentation properties of corn dough, were studied. The water absorption and weakening of corn dough increased significantly with gluten protein addition, but the development time was gradually shortened. The gas retention capacity of the dough was improved by the addition of gluten protein, which delayed the gas leakage time. The G′ and G" modulus decreased, while Tan δ increased with the increase of gluten protein content. After fermentation, corn dough with 13.65% gluten protein content had the least amount of free water. Total sulfhydryl bond content and disulfide bond content were highest in the dough with a gluten protein content of 13.65%, but irregular curling was not observed. Doughs with gluten protein content of 6.83% were less viscoelastic and elastic compared to doughs with gluten protein content of 10.24%. The gluten protein content of over 10.24% greatly improved the rheological properties of corn dough to a greater extent by strengthening the network structure of the dough.

Elevated atmospheric CO2 delays the key timing for split N applications to improve wheat (Triticum aestivum L.) protein composition.

Late stage nitrogen (N) applications following basic fertilization are commonly used to ensure grain yield and increase grain protein content in wheat. Split N applications at the late growth stage of wheat are an effective measure to improve N absorption and transport and thus increase grain protein content. However, whether split N applications can alleviate the decrease in grain protein content induced by elevated atmospheric CO2 concentrations (e[CO2]) remains unclear. In the present study, a free-air CO2 enrichment system was used to investigate the effects of split N applications (at booting or anthesis) on grain yield, N utilization, protein content, and the composition of wheat under atmospheric (ACO2; 400 ± 15 ppm) and elevated CO2 concentrations (ECO2; 600 ± 15 ppm). The results showed that wheat grain yield and grain N uptake increased by 5.0% (being grains per ear by 3.0%, 1000-grain weight by 2.0%, and harvest index by 1.6%) and 4.3%, respectively, whereas grain protein content decreased by 2.3% under ECO2 conditions. Although the negative effect of e[CO2] on grain protein content was not alleviated by split N applications, gluten protein content was enhanced due to the alteration of N distribution in different protein fractions (albumins, globulins, gliadins, and glutenins). Compared to that without split N applications, the gluten content of wheat grains increased by 4.2% and 4.5% when late stage N was applied at the booting stage under ACO2 and anthesis under ECO2 conditions, respectively. The results indicate that rational handling of N fertilizers may be a promising approach to coordinating grain yield and quality under the effects of future climate change. However, compared to ACO2 conditions, the key timing for improving grain quality by split N applications should be postponed from the booting stage to anthesis under e[CO2] conditions.

Protein Maps for Durum Wheat Precision Harvest and Pasta Production.

The quality traits of dough and dry pasta obtained from semolina (Triticum durum Desf. var. Biensur), harvested from a 13.6 ha field, subjected to variable-rate nitrogen (N) fertilization, were assessed to test site-specific pasta production for a short supply chain. Based on the grain quality spatial distribution, two distinct areas with protein content lower or higher than 13% were delineated and harvested selectively. The rheological properties of semolina samples obtained from those areas were evaluated. Furthermore, dry pasta was produced and characterized for its cooking behaviour and sensory characteristics. Semolina was demonstrated to have rheological characteristics (i.e., gluten aggregation time and energy, as evaluated by GlutoPeak test) positively related to the protein content as well as the related pasta, showing better cooking quality. These results are driven by the high amounts of gluten proteins, as well as by the glutenin/gliadin ratio, which are indicators of the technological quality of semolina. Overall, the results indicate that segregation of the grain with >13% of protein at harvest led to the production of semolina with a higher gluten protein content and a higher glutenin/gliadin ratio and, hence, to the production of pasta with better cooking quality. Therefore, site-specific pasta could be a potential asset for a short supply chain, aiming at improving traceability, as well as environmental and economic sustainability.

INFLUENCE OF THE SYSTEMATIC APPLICATION OF FERTILIZERS ON THE INTENSITY OF ACCUMULATION OF NITRATES IN AGRICULTURAL CROPS

This article examines the problems of climate change and the impact of fertilizers on the productivity and quality of winter wheat. As a result of research, it has been established that in recent decades the world has undergone significant climate change, which causes serious problems in the development of agriculture. The results of research show that the yield of winter wheat, which is obtained due to the potential fertility of soils, on average for 3 years was 2,5 t/ha. Systematic application of the fertilizers in crop rotation helped to increase the yield of winter wheat. After the treatment N60P60K60 the additions in 1,0 t/ha to the control have been received. On the plots where winter wheat was tested the aftereffect of manure 30 t/ha in combination with N60P60K60 increases the yield on 1,0 and 1,3 t/ha respectively. The highest yield of winter wheat obtained by application of mineral fertilizers in quantity N120P120K120 was 3,7 t/ha. On the control option the average protein content was 12,13. The record amount of protein observed after N120P120K120 application was 13,28. It should be noted that the quality of the gluten protein content and the winter wheat grain in all these tests is significantly higher compared with controls. The average gluten content in the control test was 20,3, while during the fertilizer using it varied in the range of 23,5–27,3. The highest protein was observed after N120P120K120 application 27,3. Besides that, the nitrate content in the grain and plants of winter wheat after different doses of mineral and organic fertilizers have been examined. It should be noted that the nitrate content in winter wheat grain is independent from the fertilizer and varied from 44,0 to 50,0 mg/kg, in the straw this dependence has been slightly observed. So if the control has 128,6 mg/kg nitrate content, in the fertilized tests it varied from 128,6 to 155,0 mg/kg. Maximum nitrate content was on the plot after the minimum N60P60K60 application and manure usage at 30 t/ha. Comparative study of the influence of mineral and organic fertilizers, as well as their combinations showed that after the application of organic fertilizer fewer nitrates accumulates in plants than after mineral and organic-mineral.

Safety Assessment of Foods and Drinks Consumed by People on a Gluten-Free Diet.

Naturally gluten-free foods and processed foods that do not contain information about the potential presence of gluten in them pose a hypothetical threat to people with food allergies and celiac disease. Patients who should follow a strict gluten-free diet do not always do so. Therefore, the aim of this research was to analyze certified “gluten-free” and naturally gluten-free products without labeled “may contain gluten” information in terms of their content of gluten proteins. The enzyme immunoassay AgraQuant Gluten G12 ELISA test kit was used for the analysis. Of all the products used in the research, only 5.8% were found to contain gluten above 20 ppm. Only one product labeled “gluten-free” was contaminated with gluten at 79.3 ppm (cider cake). In addition, our research also examined the gluten content of commercial beers containing barley malt not labeled as “gluten-free”. Research has shown that 60% of samples are not safe for those on a strict gluten-free diet. Our research clearly shows that many manufacturers, although they do not monitor their products for the presence of gluten in them, offer safe products, although they cannot be recommended in a gluten-free diet. Therefore, there is a strong need to increase the frequency of testing by food manufacturers for the presence of gluten in their products, so that the number of products approved for people on a gluten-free diet continues to increase.

Chemistry of wheat gluten proteins: Quantitative composition

AbstractBackground and ObjectivesWheat is essential to secure nutrition for the world's population. Its unique processing properties are largely determined by gluten protein content and composition.FindingsGluten proteins are subdivided into gluten protein types, α‐, γ‐, ω1,2‐, and ω5‐gliadins and high‐molecular‐weight glutenin subunits and low‐molecular‐weight glutenin subunits. The overall content and relative proportions of these types vary considerably depending on different genetic and environmental factors and mutual interactions.ConclusionThis review summarizes the latest developments related to the chemistry of gluten and how species and variety, as well as soil type, weather conditions, atmospheric CO2 concentration, diseases, and fertilization with nitrogen, sulfur, phosphorus, potassium, and other minerals affect wheat gluten protein composition.Significance and NoveltySignificant progress has been made to study the effect of different factors on gluten composition. However, comparisons between studies are almost impossible, because of the huge variability in experimental setups, environmental conditions and varieties studied. This calls for a need to develop common guidelines on how to set up experiments, on which parameters to investigate and on which procedure to use to improve comparability and reproducibility of the results.

Effects of the Foliar Application of Potassium Fertilizer on the Grain Protein and Dough Quality of Wheat

Potassium (K) has a significant effect on wheat yield and quality. Owing to the limitations of irrigation and production costs, soil-based applications of potassium fertilizer are not performed in wheat production on the Loess Plateau of China. In the late growth stage of wheat, potassium deficiency occurs even under sufficient nitrogen/phosphorus (N/P) levels, so it is necessary to supplement potassium through foliar spraying. However, there are few studies on the effect of the foliar application of potassium fertilizer (KFA) on wheat quality. Field experiments were conducted at two experimental sites for 2 years to study the effects of different potassium fertilizer application levels and periods on wheat yield and quality. The results showed that KFA had no significant effect on the yield of the wheat variety Xinong 20 (XN20) but increased the yield of the wheat variety Xiaoyan 22 (XY22). The improvement effect of KFA on the wet gluten content and stabilization time (ST) of XN20 was better than that on these parameters of XY22, while the sedimentation value (SV) and formation time (FT) showed the opposite trend. KFA significantly reduced the albumin content of the two varieties but had no significant effect on the globulin content. Compared with that at the other two stages, the potassium application in the form of potash fertilizer spray at a concentration of 60 mmol L−1 (K2) at the flowering stage (BBCH 65) significantly increased the protein content, wet gluten content, SV and gluten protein content in XN20 grains, whereas the application at 10 days after flowering (AA10, BBCH 71) at the K2 concentration was more beneficial to prolonging the dough FT. For XY22, the application of potassium fertilizer at the K2 concentration at the flowering stage increased the wet gluten and gluten protein levels and dough development time. There were significant genotypic differences in the composition and content of HMW-GS between the two varieties. KFA significantly increased the levels of the 1, 7 + 8, and 4 + 12 subunits in XN20 and the 1 subunit in XY22, but had no significant effect on the 2 + 12 subunit in XY22. Partial least squares path modelling (PLS-PM) analysis showed that the processing quality indexes (SV, FT, ST) and gluten protein and HMW-GS levels were regulated by the potassium fertilizer foliar spraying stage and concentration and revealed in part that KFA affected the processing quality by affecting the HMW-GS content.

Proteome Analysis and Epitope Mapping in a Commercial Reduced-Gluten Wheat Product.

Gluten related disorders, such as coeliac disease, wheat allergy and baker's asthma are triggered by proteins present in food products made from wheat and related cereal species. The only treatment of these medical illnesses is a strict gluten-free diet; however, gluten-free products that are currently available in the market can have lower nutritional quality and are more expensive than traditional gluten containing cereal products. These constraints have led to the development of gluten-free or gluten-reduced ingredients. In this vein, a non-GMO wheat flour that purports to contain “65% less allergenic gluten” was recently brought to market. The present study aims to understand the alteration of the proteome profile of this wheat flour material. Liquid chromatography-mass spectrometry was used to investigate the proteome profile of the novel wheat flour, which was contrasted to a wheat flour control. Using both trypsin and chymotrypsin digests and a combined database search, 564 unique proteins were identified with 99% confidence. These proteins and the specific peptides used to identify them were mapped to the wheat genome to reveal the associated chromosomal regions in the novel wheat flour and the mixed wheat control. Of note, several ω- and γ-gliadins, and low-molecular weight glutenins mapping to the short arm of chromosome 1, as well as α-gliadins from the chromosome 6 short arm were absent or expressed at lower levels in the novel wheat variety. In contrast, the high-molecular weight glutenins and α-amylase/trypsin inhibitors were notably more abundant in this variety. A targeted quantitation experiment was developed using multiple reaction monitoring assays to quantify 359 tryptic and chymotryptic peptides from gluten and related allergenic proteins revealing a 33% decrease of gluten protein content in the novel wheat flour sample in comparison to mixed wheat control. However, additional mapping of known allergenic epitopes showed the presence of 53% higher allergenic peptides. Overall, the current study highlights the importance of proteomic analyses especially when complemented by sequence analysis and epitope mapping for monitoring immunostimulatory proteins.

Gluten aggregation properties as a tool for durum wheat quality assessment: A chemometric approach

The study aims at assessing the gluten aggregation properties of wholemeal semolina for the differentiation of durum wheat cultivars with different amounts of gluten-quality related fractions. To this end, a calibration set of 56 durum wheat cultivars harvested in 2015–2016 was categorized into four quality classes based on the total gluten protein content and the glutenin to gliadin ratio. Quality class distribution was carried out by Principal Component Analysis (PCA) performed on either gluten aggregation parameters alone or with gluten fractions. PCA differentiated high and medium-high quality varieties from those of low and medium-low quality. Furthermore, PCA confirmed the relationship between gluten fractions and their aggregation kinetics, by means of a validation step consisting of 22 cultivars harvested in 2016–2017. In conclusion, gluten aggregation properties proved to be good indicators of durum wheat semolina quality, and thus a reliable tool for blend preparation in the pasta-making process.

Insight into the effect of gluten-starch ratio on the properties of Chinese steamed bread (Mantou)

Chinese steamed bread (CSB) is one of the traditional staple foods of Chinese people, and its quality is mainly affected by wheat gluten and starch. Herein, four different ratios of wheat gluten and starch were selected to investigate its effects on the properties of CSB. It was observed that the surface of CSB gradually became darker, yellower, and shrank with increasing gluten-starch ratio. The hardness and chewiness of CSB decreased with the increasing of gluten-starch ratio, as well as the network structure of CSB was dense and porous. The increase of gluten content could effectively control the migration of water in the CSB. Moreover, with increasing gluten-starch ratio, the crystallinity of starch was reduced from 9.95% to 2.03%. As a result, the ratio of gluten-starch mainly affected the development of gluten network structure and starch gelatinization through the competition of water between gluten and starch in the system, which in turn affected the quality of CSB. Thus, it will provide the basis for the adaptability of wheat flour from different origins as the raw material of CSB processing, and also provide guidance for consumers to select flour with different gluten protein content to prepare steamed bread according to their preferences.

PARTIAL WHEAT FLOUR REPLACEMENT WITH GLUTEN-FREE FLOURS IN BREAD - QUALITY, TEXTURE AND ANTIOXIDANT ACTIVITY

Consumers are increasingly looking for products in which gluten is not present or its content has been reduced by the conversion of conventional raw materials to gluten-free. The influence of the partial wheat flour replacement (from 10% to 40%) with gluten-free flours from amaranth, quinoa and millet on bread properties, including texture and crumb structure of the final product, as well as antioxidant potential and consumer acceptance were evaluated. The effect of replacement on pasting properties of flours mix was determined as well. It was found that the increased addition of millet and quinoa flours resulted in an increased maximum viscosity, whereas adding amaranth flour exerted an opposite effect. The wheat flour replacement influenced the colour of the product reducing its lightness and shifting colour balances as well. Due to a lower content of gluten proteins in the bread formulation the firmness of the crumb increased. Wholemeal flours are good source of antioxidants, antioxidant activity increased with a higher proportion of gluten-free flours.

Comparative Study on Gluten Protein Composition of Ancient (Einkorn, Emmer and Spelt) and Modern Wheat Species (Durum and Common Wheat).

The spectrophotometric Bradford assay was adapted for the analysis of gluten protein contents (gliadins and glutenins) of spelt, durum wheat, emmer and einkorn. The assay was applied to a set of 300 samples, including 15 cultivars each of common wheat, spelt, durum wheat, emmer and einkorn cultivated at four locations in Germany in the same year. The total protein content was equally influenced by location and wheat species, however, gliadin, glutenin and gluten contents were influenced more strongly by wheat species than location. Einkorn, emmer and spelt had higher protein and gluten contents than common wheat at all four locations. However, common wheat had higher glutenin contents than einkorn, emmer and spelt resulting in increasing ratios of gliadins to glutenins from common wheat (< 3.8) to spelt, emmer and einkorn (up to 12.1). With the knowledge that glutenin contents are suitable predictors for high baking volume, cultivars of einkorn, emmer and spelt with good predicted baking performance were identified. Finally, spelt, emmer and einkorn were found to have a higher nitrogen partial factor productivity than common and durum wheat making them promising crops for a more sustainable agriculture.

Gradients of Gluten Proteins and Free Amino Acids along the Longitudinal Axis of the Developing Caryopsis of Bread Wheat.

Gradients in the contents and compositions of gluten proteins and free amino acids and the expression levels of gluten protein genes in developing wheat caryopses were determined by dividing the caryopsis into three longitudinal sections, namely, proximal (En1), middle (En2), and distal (En3) to embryo. The total gluten protein content was lower in En1 than in En2 and En3, with decreasing proportions of HMW-GS, LMW GS, and α/β- and γ-gliadins and increasing proportions of ω-gliadins. These differences were associated with the abundances of gluten protein transcripts. Gradients in the proportions of the gluten protein polymers which affect dough processing quality also occurred, but not in total free amino acids. Microscopy showed that the lower gluten protein content in En1 may have resulted, at least in part, from the presence of modified cells in the dorsal part of En1, but the reasons for the differences in composition are not known.

Influence of Konjac Glucomannan and Frozen Storage on Rheological and Tensile Properties of Frozen Dough.

The impact of various amounts of konjac glucomannan on the structural and physicochemical properties of gluten proteins/dough at different periods of frozen storage is evaluated in the present study. As frozen storage time was prolonged, the molecular weight and the free sulfhydryl content of gluten proteins and the tensile properties of frozen dough all decreased. The addition of konjac glucomannan reduced the variations in the structural and rheological properties of gluten proteins/dough. Frozen dough with 2.5% added konjac glucomannan showed the highest water binding capacity and retarded the migration of water. Scanning electron microscopy and differential scanning calorimetry results also revealed that adding konjac glucomannan reduced the cracks and holes in the dough and enhanced its thermal stability. The correlations between mechanical characteristics and structure parameters further indicated that konjac glucomannan could not only stabilize the structures of gluten proteins but also bind free water to form more stable complexes, thereby retaining the rheological and tensile properties of the frozen dough.

Porovnanie vybraných genotypov pšenice, ovsa a pohánky na proteomickej úrovni

Cereals and pseudocereals are one of the most important crops. In recent years, number of people suffering from various food allergies and intolerances has grown and exactly, cereal proteins are the most common food allergen group. Pseudocereals are suitable alternative that have positive effect on human organism and do not induce allergies. The aim of this work was to analyze grains of winter wheat (Triticum aestivum L.), oat (Avena sativa) and buckwheat (Fagopyrum esculentum Moench.) and compare protein composition from the point of nutritional and technological quality. Wheat had the best technological quality based on the highest content of gluten proteins (63.42%) and HMW-GS (15.21%). Potentially allergenic wheat proteins with molecular weight in the range of 25 to 45 kDa gave strong signal in Western blot. Oat, due to the higher coefficient of nutritional quality (373.89%) and content of albumins and globulins (49.29%), had worse technological and better nutritional quality than wheat. The best nutritional quality was based on the highest coefficient of nutritional quality (6005.93%), content of albumins and globulins (60.81%) and essential amino acids in buckwheat. Buckwheat is suitable crop for gluten-free diet because it contained only 1.35% of prolamins that were not detected by Western blot analysis.

Near-Infrared Spectrum Detection of Wheat Gluten Protein Content Based on a Combined Filtering Method.

To eliminate the random error of the derivative near-IR (NIR) spectrum and to improve model stability and the prediction accuracy of the gluten protein content, a combined method is proposed for pretreatment of the NIR spectrum based on both empirical mode decomposition and the wavelet soft-threshold method. The principle and the steps of the method are introduced and the denoising effect is evaluated. The wheat gluten protein content is calculated based on the denoised spectrum, and the results are compared with those of the nine-point smoothing method and the wavelet soft-threshold method. Experimental results show that the proposed combined method is effective in completing pretreatment of the NIR spectrum, and the proposed method improves the accuracy of detection of wheat gluten protein content from the NIR spectrum.

Optimising durum wheat cultivation in North Italy: understanding the effects of site-specific fertilization on yield and protein content

Variable rate fertilization and precision harvesting could increase the potential for meeting durum wheat quality standards. Field spatial distribution of yield and protein content, and their interactions with soil properties and N fertilization were evaluated in an experiment on durum wheat in North Italy in 2011 and 2012. Variable rate fertilization was adopted in three management zones (MZs) with increasing soil fertility, and a foliar N was applied at flowering to investigate differences in protein quantity and quality. During the crop cycle, changes in crop biomass and N status were monitored through NDVI measurement, and grain was sampled in each MZ and gluten proteins extracted at harvest. Spatial variability of yield and protein content was mainly driven by soil texture and base fertilization in both the years, while foliar fertilization was not efficient in enhancing grain protein content. Variable rate fertilization partially mitigated the weather impact; however, unpredictable weather conditions resulted in low N use efficiency. High N rates were confirmed to provide high protein levels and enhance gluten proteins technological quality, but with a risk for the environment. The marked spatial variability in grain quality in terms of total protein and gluten protein content, and the ratio between glutenin/gliadin and high and low-molecular weight glutenin sub-units, suggested the implementation of zone harvesting as a strategy to exploit the positive interaction between grain quality and soil fertility.

Flax - Evaluation of composite flour and using in cereal products

Two types of yellow and brown linseed, differing in granulation, were tested in form of wheat flour composites (additions 2.5% and 5.0%) by using the Farinograph, the Extensigraph and the Rapid Visco Analyser (RVA) apparatuses. Additions of brown and yellow flax fibre significantly affected Falling Number and Zeleny test values. Curves of farinograph were differed according to flax fibre type - finer flax (better terminology) granulation meant somewhat stronger negative changes in dough stability and dough softening degree. Results of extensigraph test demonstrated changes in dough elasticity and extensibility due to lowering of gluten protein content. Appearance of the RVA profiles was verifiably different, reflecting diverse wheat and flax polysaccharides, added dietary fibre type and its granulation. Due to that, bread volume and shape was lowered up to one-half in case of golden flax composites. Similar tendencies with smaller negative influence caused the brown linseed. Fibre from flax is used for technical (textile) use, but linseed dietary fibre addition affected quality of laboratory prepared cut-off biscuits and dried pasta differently, showing a dependence on the fibre type, granulation as well as addition level. Sensory profiles of all mentioned product types were acceptable.