Gluten Cereals Research Articles

Pica Syndrome Revealing Celiac Disease: A Case Report

Celiac disease is an autoimmune mediated chronic inflammatory enteropathy primarily affecting the small intestine, occurring in genetically predisposed individuals and caused by a permanent sensitivity to ingest gluten cereals. Pica is the compulsive eating of nonnutritive substances, and may be either a cause or a result of iron deficiency, with the latter being a possible presenting sign of Celiac Disease. We report the case of an 18-year-old female patient with history of depression and pica disorder. Laboratory investigations, serology testing and duodenal biopsy showed arguments of celiac disease with spectacular improvement noted after initiating the gluten free diet.

Wpływ diet eliminacyjnych na jakość życia osób z wybranymi chorobami autoimmunologicznymi

Złożoność etiopatogenezy schorzeń autoimmunologicznych, w tym celiakii i choroby Hashimoto oraz brak skutecznej farmakoterapii powoduje, że istotnym czynnikiem poprawy stanu zdrowia i komfortu życia oraz zmniejszenia rozwoju powikłań może być odpowiednia dietoterapia. W badaniach własnych zaobserwowano, że jedna trzecia osób z celiakią oprócz wyłączenia zbóż glutenowych eliminowała z diety produkty mleczne. Większość osób z chorobą Hashimoto wyłączyła z diety zboża glutenowe, a ponad połowa również produkty mleczne i soję. Największą poprawę stanu zdrowia, w tym szczególnie zmniejszenie występowania nudności, wzdęć, dolegliwości bólowych oraz zwiększenie uczucia komfortu obserwowały osoby z celiakią eliminujące oprócz zbóż glutenowych: mleko i jego przetwory, orzechy i soję. Podobnie największe zmiany samopoczucia związanego z objawami choroby Hashimoto, w tym szczególnie zmniejszenie nadwrażliwości na temperaturę, uczucia suchości skóry oraz poprawę samopoczucia deklarowały osoby eliminujące zboża glutenowe, produkty mleczne, orzechy arachidowe i warzywa psiankowate. Leczenie celiakii oparte na diecie bezglutenowej połączone z eliminacją orzechów i soi może przynieść największe korzyści związane z poprawą stanu zdrowia i jakości życia, a eliminacja zbóż glutenowych i orzeszków ziemnych może wspomagać poprawę jakości życia osób z chorobą Hashimoto.
 Słowa kluczowe: dieta eliminacyjna, Hashimoto, celiakia

Production of free-gluten bread from some cereal crops

Bread is an important source of nutrition around the world. But there are a large number of people have gluten sensitivity which found in wheat and barley, so the manufacture of bread from free- gluten cereal is essential for these people. This study concerned with the production of free- gluten flat bread using corn and rice, in addition to a new crop that has recently cultivated in Egypt, which is the quinoa crop due to its high nutritional value and free of gluten. Flat bread was produced using mixture of 100% extraction rate of quinoa, 100% extraction rate of white rice flour and 97% extraction rate of yellow corn flour. Blends were prepared by adding them in different proportions. Physicochemical properties were studied using chemically analyzed for both row materials and different blends. The sensory evaluation and the staling were conducted to the flat bread in order to determine the best blend. By studying the sensory evaluation and the staling, it became clear that the blends of quinoa flour supplemented by white rice flour is the best. On the other hand, the increasing of the ratio of the yellow corn in the blends leads to decreasing the freshmen of the flat bread. While, the existence of the white rice flour in the blends decreases the effect of the yellow corn flour.

Occurrence and potential transfer of mycotoxins in gilthead sea bream and Atlantic salmon by use of novel alternative feed ingredients

Plant ingredients and processed animal proteins (PAP) are suitable alternative feedstuffs for fish feeds in aquaculture practice, although their use can introduce contaminants that are not previously associated with marine salmon and gilthead sea bream farming. Mycotoxins are well known natural contaminants in plant feed material, although they also could be present on PAPs after fungi growth during storage. The present study surveyed commercially available plant ingredients (19) and PAP (19) for a wide range of mycotoxins (18) according to the EU regulations. PAP showed only minor levels of ochratoxin A and fumonisin B1 and the mycotoxin carry-over from feeds to fillets of farmed Atlantic salmon and gilthead sea bream (two main species of European aquaculture) was performed with plant ingredient based diets. Deoxynivalenol was the most prevalent mycotoxin in wheat, wheat gluten and corn gluten cereals with levels ranging from 17 to 814 and μgkg−1, followed by fumonisins in corn products (range 11.1–4901μgkg−1 for fumonisin B1+B2+B3). Overall mycotoxin levels in fish feeds reflected the feed ingredient composition and the level of contaminant in each feed ingredient. In all cases the studied ingredients and feeds showed levels of mycotoxins below maximum residue limits established by the Commission Recommendation 2006/576/EC. Following these guidelines no mycotoxin carry-over was found from feeds to edible fillets of salmonids and a typically marine fish, such as gilthead sea bream. As far we know, this is the first report of mycotoxin surveillance in farmed fish species.

The effects of reduced gluten barley diet on humoral and cell-mediated systemic immune responses of gluten-sensitive rhesus macaques.

Celiac disease (CD) affects approximately 1% of the general population while an estimated additional 6% suffers from a recently characterized, rapidly emerging, similar disease, referred to as non-celiac gluten sensitivity (NCGS). The only effective treatment of CD and NCGS requires removal of gluten sources from the diet. Since required adherence to a gluten-free diet (GFD) is difficult to accomplish, efforts to develop alternative treatments have been intensifying in recent years. In this study, the non-human primate model of CD/NCGS, e.g., gluten-sensitive rhesus macaque, was utilized with the objective to evaluate the treatment potential of reduced gluten cereals using a reduced gluten (RG; 1% of normal gluten) barley mutant as a model. Conventional and RG barleys were used for the formulation of experimental chows and fed to gluten-sensitive (GS) and control macaques to determine if RG barley causes a remission of dietary gluten-induced clinical and immune responses in GS macaques. The impacts of the RG barley diet were compared with the impacts of the conventional barley-containing chow and the GFD. Although remission of the anti-gliadin antibody (AGA) serum responses and an improvement of clinical diarrhea were noted after switching the conventional to the RG barley diet, production of inflammatory cytokines, e.g., interferon-gamma (IFN-γ), tumor necrosis factor (TNF) and interleukin-8 (IL-8) by peripheral CD4+ T helper lymphocytes, persisted during the RG chow treatment and were partially abolished only upon re-administration of the GFD. It was concluded that the RG barley diet might be used for the partial improvement of gluten-induced disease but its therapeutic value still requires upgrading—by co-administration of additional treatments.

A trichobezoar in a child with undiagnosed celiac disease: A case report

Celiac disease is a chronic, immune-mediated enteropathy caused by a permanent sensitivity to ingested gluten cereals that develops in genetically susceptible individuals. The classic presentation of celiac disease includes symptoms of malabsorption but has long been associated with cognitive, emotional, and behavioral disorders. We describe an 8-year-old patient with non-scarring alopecia and diagnosed with trichotillomania. Furthermore, she presented with a 3-year history of poor appetite and two or three annual episodes of mushy, fatty stools. Laboratory investigations showed a normal hemoglobin concentration and a low ferritin level. Serologic studies showed an elevated tissue immunoglobulin G anti-tissue transglutaminase level. A duodenal biopsy showed subtotal villous atrophy and crypt hyperplasia, and a large gastric trichobezoar was found in the stomach. Immediately after beginning a gluten-free diet, complete relief of trichotillomania and trichophagia was achieved. In this report, we describe a behavioral disorder as a primary phenomenon of celiac disease, irrespective of nutritional status.

Bioavailability of lysine in selected foods by rat growth assay

Lysine bioavailabilities in reference protein and 16 test protein diets were estimated using 10 day rat growth assays. A standard growth curve was obtained by feeding 5 diets containing casein, zein and synthetic amino acids ranging in total lysine concentration from 0.3 to 0.7%. Experimental foods were added to the basal diet at the expense of zein and/or synthetic amino acids to provide 2 specific lysine concentrations, i.e., 0.4 and 0.6%. Availabilities were established by comparing growth responses from the test food diets to the regression line of the standard growth data. Availabilities were over 88% for 13 of 16 products. Utilization was poor in pinto beans (73%), rice-wheat gluten cereal (70%), and skim milk powder heated to 100 degrees C for 12 h (66%). Addition of excess lysine (700 mg/100 g diet) to the pinto bean diet did not improve growth response; thus poor digestibility or some unidentified growth inhibitor is indicated.

Digestibility of protein and amino acids in selected foods as determined by a rat balance method.

Values (%) for true digestibility of crude protein and individual amino acids in 20 selected foods were determined by the rat balance (fecal) method. The products were fed as the sole source of protein in diets containing 8% crude protein (N x 6.25). Lowest true protein digestibility values (79-84) were obtained for pinto beans, kidney beans and lentils; intermediate values (89-92) were obtained for chick peas, beef stew, skim milk (over heated), rolled oats, whole wheat cereal, and pea protein concentrate; and highest values (94-100) were obtained for sausage, macaroni-cheese, rice-wheat gluten cereal, skim milk, tuna, soy isolate, peanut butter, chicken frankfurters, beef salami, casein and casein + methionine. In animal foods, peanut butter and soy isolate, the differences between true digestibility of crude protein and most individual amino acids were less than 5%. However, the values for true digestibility of methionine and cystine were up to 44% lower than those of crude protein in pinto beans, kidney beans, lentils, chick peas and pea concentrate. In these legumes, digestibility of crude protein was not a good predictor of digestibility of the limiting amino acids.

Relationship between amino acid scores and protein quality indices based on rat growth

Protein efficiency ratio (PER), relative PER (RPER), net protein ratio (NPR) and relative NPR (RNPR) values, and amino acid scores were calculated for 20 food products (casein, casein + Met, beef salami, skim milk, tuna, chicken frankfuters, sausage, heated skim milk, peanut butter, rolled oats, soy isolate, chick peas, pea concentrate, kidney beans, wheat cereal, pinto bean, lentils, rice-wheat gluten cereal, macaroni-cheese, and beef stew). In most cases, PER, RPER, NPR or RNPR ranked the products in the same order and positive correlations among the protein quality methods were highly significant (r = 0.98-0.99). Amino acid scores (based on the first limiting amino acid, Lys-Met-Cys, Lys-Met-Cys-Trp or lys-Met-Cys-Trp-Thr) were positively correlated to the PER, RPER, NPR or RNPR data (r = 0.61-0.75). Inclusion of the correction for true digestibility of protein improved the correlations between amino acid scores and the indices based on rat growth. The correlations were especially high between Lys-Met-Cys scores (corrected for true digestibility of protein) and PER, RPER, NPR or RNPR (r = 0.86-0.91). Inclusion of the correction for true digestibility of individual amino acids did not result in further improvements of the correlations in most cases. It is concluded that adjusting amino acid scores for true digestibility of protein would be sufficient and further correction for digestibility of amino acids would be unnecessary in mixed diets.

Protein efficiency ratios and net protein ratios of selected protein foods.

As a part of a cooperative study initiated to assess both in vitro and in vivo protein quality methods, the protein efficiency ratio (PER) and net protein ratios (NPR) of 15 different protein sources were determined. Male weanling Sprague-Dawley rats were fed a 10% protein diet. Fourteen-day NPR and relative NPR (RNPR) values and 14- and 28-day PER and relative PER (RPER) values were calculated for each protein source. When protein quality values were expressed relative to ANRC casein, the 14- and 28-day PER data ranked the protein sources essentially in the same order. RPER values of nonfat dried skim milk (unheated) and tuna were more than 100% that of casein; nonfat dried skim milk (heated), chickpeas, and breakfast sausage were between 50 and 70% of that of casein; and pinto beans and rice-wheat gluten cereal did not support substantial growth of the rat. The NPR method did not always rank the protein sources in the same order as the PER method. For the poor quality proteins, RNPR values were much higher than the RPER values; however, the RNPR and RPER values agreed closely for high quality protein sources.