• Regeneration of Clivia via somatic embryogenesis was reported for the first time. • A reproducible and simple protocol for Clivia propagation was established. • The reproduction coefficient of Clivia has been greatly improved. Clivia miniata Regel. is a world-famous ornamental and medicinal plant species. Expanding the demand for commercial Clivia cultivation requires a reliable, highly efficient, stable system for reproduction. In this study, an efficient protocol for Clivia in vitro regeneration through somatic embryogenesis via a TCL technique was established. Clivia regeneration systems were optimized for commercial application. Under complete darkness, MS media supplemented with 0.5 mg/L NAA and 1.0 TDZ induced callus production from hypocotyls, and the callus induction rate was 25.58%. The calli were then transferred to MS media + 0.5 mg/L NAA + 0.5 mg/L TDZ media and allowed to grow for 60 days, and the callus proliferation rate reached 217%. Organogenic calli inoculated in MS media + 0.5 mg/L NAA + 2.0 mg/L BAP produced adventitious shoots, and the induction rate was 81.11%. The shoots were transferred to rooting media (1/2-strength MS media+0.5 mg/L NAA); they produced roots after 45 days, and the rooting rate was 100%. Moreover, somatic embryogenesis of Clivia could be directly induced by the use of 1 mm root TCLs of in vitro plants. In the presence of 2.0 mg/L PIC, the formation of globular embryos could be directly induced from the vascular bundles of TCLs at 45 days after inoculation. An average of 10∼16 globular embryos were induced per TCL. After approximately 4 months, the somatic embryos developed into plantlets. Our in vitro propagation protocol could be used for sustainable commercial utilization as well as conservation of Clivia bioresources.