Reducing drag is critical to aircraft design. In recent years, laminar technology has become one of the most important feasible technologies for civil aircraft drag reduction design under many design constraints. However, various factors have a certain impact on the laminar flow characteristics in the state of transonic flight. Therefore, it is necessary to deeply understand the specific effects of various flight parameters on the characteristics of laminar flow. In this paper, a parameter sensitivity analysis for a central experimental wing in a special layout aircraft was carried out to investigate its transonic laminar characteristics. Then, the airfoil of the central experimental wing of the aircraft was designed for real flight. The RANS (Reynold-averaged Navier–Stokes) method combined with the γ−Reθ transition model based on local variables was used. The computational approach was validated by the wind tunnel tests and analyzed by the grid independence analysis. The sensitivity mainly focuses on the transition location and the length of the laminar flow zone of the central experiment under different boundary conditions. The transonic transition was affected by a variety of interacting factors that include FSTI (free stream turbulence intensity), pressure gradient, Re (Reynolds number), Ma (Mach number) and α (angle of attack, degree). The essence of the transition is the disruption of flow stability caused by the increase in flow entropy. Among these factors, FSTI directly affects global flow stability, and the pressure gradient affects local flow stability. Ma and α can indirectly affect the flow stability by changing the pressure gradient. Re can control the boundary layer properties to change the flow stability, whereas its effect is easily determined by the pressure gradient. Finally, the improved design of the airfoil with the central experimental wing was conducted. The design of weak shock wave and aerodynamic load on the rear part of the airfoil can improve the aerodynamic characteristics (CL, lift coefficient, increases by 0.28) of the airfoil, which can reduce the load burden on the outboard wing without affecting the laminar flow characteristics of the airfoil. In the next step, cross-flow instability will be considered.