Number Of Species Research Articles

Boosting biodiversity monitoring using smartphone-driven, rapidly accumulating community-sourced data.

Comprehensive biodiversity data is crucial for ecosystem protection. The Biome mobile app, launched in Japan, efficiently gathers species observations from the public using species identification algorithms and gamification elements. The app has amassed >6 million observations since 2019. Nonetheless, community-sourced data may exhibit spatial and taxonomic biases. Species distribution models (SDMs) estimate species distribution while accommodating such bias. Here, we investigated the quality of Biome data and its impact on SDM performance. Species identification accuracy exceeds 95% for birds, reptiles, mammals, and amphibians, but seed plants, molluscs, and fishes scored below 90%. Our SDMs for 132 terrestrial plants and animals across Japan revealed that incorporating Biome data into traditional survey data improved accuracy. For endangered species, traditional survey data required >2000 records for accurate models (Boyce index ≥ 0.9), while blending the two data sources reduced this to around 300. The uniform coverage of urban-natural gradients by Biome data, compared to traditional data biased towards natural areas, may explain this improvement. Combining multiple data sources better estimates species distributions, aiding in protected area designation and ecosystem service assessment. Establishing a platform for accumulating community-sourced distribution data will contribute to conserving and monitoring natural ecosystems.

Sustained ecological impacts of invasive crayfish following claw injury

ABSTRACT Human activities have significantly accelerated the translocation of species outside their native range with severe consequences for global biodiversity. Freshwater crayfish are a particularly successful group of invasive species, as exemplified by the increasingly widespread marbled crayfish Procambarus virginalis Lyko, 2017. A significant portion of crayfish populations has regenerating or missing claws. At the same time, claws play important roles in crayfish feeding and intraspecific interactions, altering their ability to invade new areas and impact native communities. Here, using functional response analyses, we combined laboratory experiments and modeling to investigate whether the number of claws on marbled crayfish modulates its ecological impact (focusing on predation rate) as well as its intraspecific interactions via population dynamic modeling. We found that the number of claws did not affect the marbled crayfish’s functional response type (hyperbolic type II), attack rates, or handling times when preying on chironomid larvae. Rates of partial prey consumption were overall low (<2%), and claw number had a significant effect on the partial consumption rate of prey, which tended to increase with the number of claws and prey density. The presence of multiple intraspecific predators influenced marbled crayfish non-trophic behavior, with antagonistic effects prevalent between crayfish pairs regardless of claw status. Moreover, the impact of multiple predators was prey density-dependent, with the highest levels of antagonism shown at the lowest prey density across crayfish groups. Our findings indicate that the ecological influence of invasive crayfish remains unchanged by the number of claws, highlighting the escalating, context-independent threat these species pose to freshwater ecosystems.

Cities in nature.

More than half of the world's population live in cities, and they have benefitted from the ecosystem services provided by urban biodiversity. International conventions like the Convention on Biological Diversity have provided recommendations on how to engage local governments and cities in the implementation of their strategic plans. In parallel, recognizing this, cities conserve, restore and enhance biodiversity in many practical ways. A list of general strategies adopted by cities is presented. The rich and diverse experiences of eleven cities, i.e. Abu Dhabi, Auckland, Durban, Edinburgh, Edmonton, Hyderabad, Los Angeles, São Paulo, Singapore, Toluca and Vitoria-Gasteiz, which have successfully conserved biodiversity in urbanized landscape across the globe, are shared. These cities have all applied the Singapore Index on Cities' Biodiversity as an evaluation and monitoring tool for biodiversity conservation efforts, in addition to using it innovatively for other purposes. Cities can play a pivotal role in ensuring that Target 12 of the Kunming-Montreal Global Biodiversity Framework will be achieved. Upscaling cities' models of success would require a worldwide concerted effort involving everyone, i.e. all levels of stakeholders. Mainstreaming of biodiversity into all sectors, including commercial, economic, financial, industrial and technological, and the incorporation of biodiversity into decision-making in urban planning and management would be a game-changer. This article is part of the theme issue 'Bringing nature into decision-making'.

The Global Biodiversity Framework can be leveraged to better manage transboundary watersheds

The Global Biodiversity Framework can be leveraged to better manage transboundary watersheds

Recircumscription of the genera Goniozus and Sierola (Hymenoptera: Bethylidae) based on morphological and molecular evidence

A number of undescribed species of the bethylid genera Goniozus and Sierola have been discovered in Australia and North America with character states that had previously been thought to be exclusive to one or the other, prompting a re-examination of the generic boundaries. Analysis of a large dataset of cytochrome oxidase I mtDNA sequences and key morphological characters found that Goniozus is well defined by two insertion-deletion events in COI. The traditional morphological characters can also be used to separate the genera, but variation within the regional fauna must be taken into account. Sierola always has four maxillary palpomeres; Goniozus usually has five, but some Nearctic species have four. Goniozus always has cell 2R1 of the fore wing open, with vein Rs weakly curved; Sierola has Rs bent sharply towards the wing margin, usually closing 2R1, but a small number of recently-described Hawaiian species and many undescribed Australian species and have it narrowly to moderately open. As a result of these changes, two species are transferred from Goniozus to Sierola: Sierola angulata (Muesebeck, 1933) comb. nov. from Australia, and Sierola jamiei (Ward, 2013) comb. nov. from New Zealand.

Understanding diversity-synchrony-stability relationships in multitrophic communities.

Understanding how species loss impacts ecosystem stability is critical given contemporary declines in global biodiversity. Despite decades of research on biodiversity-stability relationships, most studies are performed within a trophic level, overlooking the multitrophic complexity structuring natural communities. Here, in a global analysis of diversity-synchrony-stability (DSS) studies (n = 420), we found that 74% were monotrophic and biased towards terrestrial plant communities, with 91% describing stabilizing effects of asynchrony. Multitrophic studies (26%) were representative of all biomes and showed that synchrony had mixed effects on stability. To explore potential mechanisms, we applied a multitrophic framework adapted from DSS theory to investigate DSS relationships in algae-herbivore assemblages across five long-term tropical and temperate marine system datasets. Both algal and herbivore species diversity reduced within-group synchrony in both systems but had different interactive effects on species synchrony between systems. Herbivore synchrony was positively and negatively influenced by algal diversity in tropical versus temperate systems, respectively, and algal synchrony was positively influenced by herbivore diversity in temperate systems. While herbivore synchrony reduced multitrophic stability in both systems, algal synchrony only reduced stability in tropical systems. These results highlight the complexity of DSS relationships at the multitrophic level and emphasize why more multitrophic assessments are needed to better understand how biodiversity influences community stability in nature.

Biodiversity Characteristics and Carbon Sequestration Potential of Successional Woody Plants Versus Tree Plantation under Different Reclamation Treatments on Hard-Coal Mine Heaps––A Case Study from Upper Silesia

In the discussion about sustainable forestry, a key role is played by the development of ecosystem services, including ecological, social, and economic ones, in which biodiversity and carbon (C) sequestration are among the most important. Afforestation of disturbed and post-mining sites is one of the ways to minimize the negative impact of civilization on the environment. Optimizing C sequestration strategies at post-mining sites plays a crucial role in promoting ecosystem recovery, supporting climate change mitigation, and enabling C offsetting. In this study, we compared the C storage in the soil and plant biomass of forest ecosystems developed on coal-mine heaps for different scenarios of reclamation and succession. We tested combinations of sites (i.e., non-reclaimed sites on bare carboniferous rock [BR] and sites reclaimed by applying topsoil [TS]) and successional woodland and tree plantation. The estimated potential for total C storage (in the soil + biomass) for TS sites ranged from 68.13 to 121.08 Mg ha−1, of which 52.20–102.89 Mg ha−1 was stored in the soil and 12.09–20.15 Mg ha−1 in the biomass. In the non-reclaimed sites on BR, the total C storage was much higher, amounting to 523.14 Mg ha−1 (507.66 Mg ha−1 being in the soil), which was due to the geogenic coal content in the BR. However, the C storage in the biomass (15.48 Mg ha−1) and litter (5.91 Mg ha−1) was similar to the amounts obtained from the reclaimed sites. The number of species did not differ statistically significantly between the analyzed variants. On average, 14 species were recorded in the plots. The average Shannon–Wiener index (H’) value was higher for sites with BR (1.99) than TS variants on reclaimed plots (1.71). The lowest H’ value was for those plots with Robinia pseudacacia in the stand. One of the main implications of the obtained results for sustainable forestry is the perspective of using succession in the recovery of a disturbed ecosystem. We noted that woodlands from succession on BR are highly biodiverse, have high C sequestration potential, and do not require time-consuming reclamation treatments.

Description of two new species of Claustropyga Hippa, Vilkamaa &amp; Mohrig (Diptera, Sciaridae) from the northern Palaearctic

Claustropyga alveata sp. n. and C. caetrata sp. n. from the northernmost Palaearctic are described and illustrated. Claustropyga alveata sp. n. has a highly modified tegmen and unusual gonocoxal apodemes, and C. caetrata sp. n. has an uncommonly shaped apical tooth of the gonostylus. The newly described species raise the number of known species of Claustropyga to twenty-seven.

Unique microbial communities of parasitic fleas on wild animals from the Qinghai-Tibet Plateau.

Fleas, one of the most significant ectoparasites, play a crucial role as vectors in spreading zoonotic diseases globally. The Qinghai Province, as part of the Qinghai-Tibet Plateau, is one of the provinces in China with the largest number of flea species. In this study, we characterized the microbial communities of eighty-five adult fleas, belonging to nineteen species within four families (Ceratophyllidae, Ctenophthalmidae, Leptopsyllidae, and Pulicidae). We identified a total of 1162 unique operational taxonomic units at the genus level, with flea-borne pathogens such as Wolbachia, Bartonella, Rickettsia being the members of top abundant taxa. Except for comparison between Ctenophthalmidae and Leptopsyllidae families, the analyses of both alpha- and beta- diversity indicators suggested that bacterial diversity varied among flea families. This could be attributed to flea phylogeny, which also influenced by their geographical sites and animal hosts. Results of Linear discriminant analysis effect size (LEfSe) indicated that 29 genera in Ceratophylloidea, 11 genera in Ctenophthalmidae, 15 genera in Leptopsyllidae, and 22 genera in Pulicidae were significantly responsible for explaining the differences among the four flea families (linear discriminant analysis score > 2, P < 0.05). Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) analyses showed that the functional pathways varied significantly across flea families, which was supported by the significant correlation between the functional pathways and the microbial communities.

Gastropods are faced with potential environmental hazards in Kuala Langsa, Aceh

The Kuala Langsa Peninsula is a geographical region that is often developed as a site of habitat. The surrounding nature of Kuala Langsa is an environment of biodiversity that contains gastropods. There are also several mangrove areas throughout Kuala Langsa with certain characteristics, under certain conditions and status, which are ecological or non-ecological conservation. The purpose of this investigation is to determine the level of pollution that occurs in the port of Kuala Langsa by looking at the value of the index of gastropodic diversity. The research uses the quantitative method with the Shannon-Wienner diversity index formula and sampling using the square tranche technique. The results show that diversity from common and detected low quality is based on the total number of species in a community found, as well as very similar types of organisms, resulting in low diversity coefficient values and water quality belonging to moderate pollution. The conclusion is that the total number of gastropods is 1,064 individuals from 9 families and 19 species on the three research locations. It can be concluded that the highest value of the diversity index obtained with the data is at station II, which is 2, whereas the lowest diversity Index value at station III is at 1.86 and is followed by station I at 1.91, as long as from the data the variability index value belongs to the type of moderate polluted waters.

Tinkering While the Arctic Marine Environment Totters: Governance and the Triple Polar Crisis$

After describing how the marine environment is tottering in the face of the triple environmental crisis, this article explores the limited governance progressions at the global and regional levels in addressing the threats of pollution, climate change and biodiversity loss in the especially vulnerable Arctic. For pollution, key limitations include slow and arduous processes to add chemicals for control under the Stockholm and Rotterdam Conventions and reliance at the regional level on a fragmented array of pollution studies and projects but without specific region-wide legally binding pollution standards. For climate change, the world is not on track to meet the Paris Agreement’s temperature targets which is especially problematic for the Arctic cryosphere while the Arctic Council has largely been limited to providing general statements of concern and aspirational calls for enhanced climate mitigation and adaptation actions. For marine biodiversity losses, a pan-Arctic network of marine protected areas has yet to be developed and various implementation challenges surround the Agreement to Prevent Unregulated High Seas Fisheries in the Central Arctic Ocean including the need to ensure adequate financial, human resource and technical support. The paper concludes by highlighting some promising future governance directions. They include: the conclusion of a global treaty on plastic pollution; implementation of a new Global Framework on Chemicals – For a Planet Free of Harm from Chemicals and Waste; expected further clarifications from international tribunals on State responsibilities to address climate change; and regional implementation of the Kunming-Montreal Global Biodiversity Framework and the new agreement under the UN Convention on Law of the Sea on the conservation and sustainable use of marine biological diversity of areas beyond national jurisdiction.

Phylogeny and evolution of streptophyte algae.

The Streptophyta emerged about a billion years ago. Nowadays, this branch of the green lineage is most famous for one of its clades, the land plants (Embryophyta). While Embryophyta make up the major share of species numbers in Streptophyta, there is a diversity of likely more than 5000 species of streptophyte algae that form a paraphyletic grade next to land plants. Here, we focus on the deep divergences that gave rise to the diversity of streptophytes-and thus, particularly on the streptophyte algae. Phylogenomic efforts have not only clarified the position of streptophyte algae to land plants but recent efforts have also begun to unravel the relationships and major radiations within streptophyte algal diversity. We illustrate how new phylogenomic perspectives have changed our view on the evolutionary emergence of key traits such as intricate signaling networks that are intertwined with multicellular growth and the chemodiverse hotbed from which they emerged. These traits are key for the biology of land plants-but were bequeathed from their algal progenitors.

Environmental Factors Structuring Diatom Diversity of the Protected High Mountain Lakes in the Kaçkar Mountains National Park (Rize, Turkey)

The altitude of the habitat is one of the important regulators of species survival. Kaçkar Mountains National Park is located in the Eastern Black Sea region of Turkey. This is the first study on the benthic diatom flora of the high mountain lakes in Kaçkar Mountains National Park, which is situated between 2782 and 3075 m a.s.l. A total of 84 diatom species were identified from benthic communities of 15 habitats in summer (19 July, 28 August) and autumn (10 September) months of 2020. The genus Pinnularia (thirteen species) formed the basis of the taxonomic list, followed by Eunotia (five species), Navicula (five species), and Frustulia (four species) genera, respectively. The waters in all the studied lakes were fresh, low-saline, with low-alkaline or circumneutral pH and organically uncontaminated, as evidenced by prevailed bioindicator groups. Statistical methods and comparative floristic results confirm the role of the lake altitude for the diatom species distribution. The species richness of the studied lakes was higher in lakes with lower altitudes. The statistical approach also revealed the potential for an increase in the number of species in high mountain lakes if the study of the diatom flora of the Kachkar Mountains National Park is continued and the species composition of the lakes is replenished. Further studies will be needed to continue exploring this pattern. To protect studied high mountain lakes, their ecological conditions must be constantly monitored in the Kaçkar Mountains National Park.

Poor convergence between local traditional farmers and conservationists on which species to protect locally

Abstract Locals engaged in traditional farming and possessing traditional ecological knowledge consider certain species worthy of protection, as do official nature conservationists, although the sets of taxa may not be identical. Exploring the relationship between the two sets of taxa could bring many practical benefits, yet the literature on this subject is scarce. For more efficient conservation and better engagement and knowledge co‐production with locals, it is necessary to understand the principles, preferences and worldviews of the two knowledge systems, and the drivers behind the choices of which animal species to protect. We examined which animal species traditional farmers and conservationists wish to protect, and why. We also examined whether there is a correlation between the extent of farmers' ecological knowledge and the number of species they regard as needing protection. In the case of the species that conservationists consider most in need of protection, we also enquired how knowledgeable local farmers are about these species. Our research was carried out in two adjacent protected sites in Central Europe along the Slovenian‐Hungarian border. We conducted 20 structured interviews with traditional farmers at each of the two sites (40 altogether), and 23 with local conservationists. Both conservationists and local farmers predominantly mentioned the protection of species that do not provide a tangible economic benefit to farmers and that show a declining population trend. Local farmers with greater species knowledge did not know significantly more than those with less knowledge about the species to be protected, nor did they list more legally protected species. The preliminary assumption of the conservationists was that the locals knew the species and listed the ones to be protected for essentially functional reasons (e.g. usefulness). By contrast, it was found that many more aspects (e.g. population trends, appearance) also had a significant impact. Once the boundaries between the two knowledge systems are removed, collaboration between the stakeholder groups can facilitate the protection of natural assets and local communities. We consider it the responsibility of conservationists (together with ethnoecologists and other researchers) to ensure that these preferences are properly understood for the benefit of conservation and local communities. Read the free Plain Language Summary for this article on the Journal blog.

Does Exposure to Burning and Heated Tobacco Affect the Abundance of Perio-Pathogenic Species in the Subgingival Biofilm?

This study investigated the impact of tobacco exposure, specifically through heating and burning, on periodontopathogens in the subgingival microbiome among clinically healthy individuals and those diagnosed with periodontitis. The sample comprised 66 subjects (26–56 years, median 38 yrs; 64% females) classified as non-smokers, classic cigarette smokers, and tobacco heating system (THS) smokers (each N = 22). Full-mouth periodontal examination was performed, and 330 paper-point samples from periodontal pockets were collected. Next-generation sequencing of 16S rRNA genes was conducted to identify the composition of subgingival microbiome. Periodontitis prevalence among the groups was ranked as THS (41%) &lt; non-smokers (44%) &lt; cigarette smokers (68%), without statistically significant differences between the groups. The number of perio-pathogenic species was higher in subjects with periodontitis compared to those without (median 7 vs. 6 species; p = 0.005) but without significant differences between exposure groups: non-smokers (6) = smokers (6) &lt; THS (6.5). When combining exposure and periodontal status, each smoker group had more perio-pathogenic species than non-smokers: non-smokers without periodontitis (5) &lt; smokers without periodontitis (5.5) &lt; THS without periodontitis (6); non-smokers with periodontitis (6.5) &lt; THS with periodontitis (7) = smokers with periodontitis (7). Multiple linear regression indicated periodontitis as the sole predictor of perio-pathogenic species quantity, irrespective of the type of tobacco consumption, sex, age, or oral hygiene (R2 = 0.163; p = 0.005). In conclusion, the quantity of perio-pathogenic species in the subgingival microbiome was more influenced by periodontitis than by exposure to tobacco smoke, regardless of whether it was heated or burned.

Secondary Succession in Fallow Agroforestry Systems Managed in Tropical Dry Forest in Western Mexico

Tropical dry forests (TDFs) are ecosystems of high biocultural value, in which agroforestry systems (AFSs) have been essential in their management and conservation. We aimed to characterize agroforestry practices and analyze their capacity to conserve perennial plant diversity. In addition, we sought to evaluate how the management of TDFs as AFSs, together with their regeneration, influences species diversity and vegetation structure in a landscape with AFSs and TDFs in different conservation states. We compared the species diversity and basal area (BA) of plants in active and fallow AFSs at different regeneration stages in Zacualpan, Colima, Mexico. We found that AFSs harbored 71% of species richness (0D), forming a mosaic that contributed to the gamma diversity (124 species) of TDFs in the area. AFSs supported 23 endemic and 12 protected species. TDFs, active and advanced regeneration AFSs, had the highest number of useful species and diversity. Species richness (0D) in management categories increased as succession progressed, but not the BA, possibly due to frequent browsing and wood and firewood extraction. However, BA may be related to the management of useful trees maintained through agroforestry practices. We suggest increasing the matrix quality through a mosaic of active and fallow AFSs to promote ecological connectivity and biodiversity conservation.

Laboratory evaluation of different eco-friendly insecticides against the Egyptian fluted scale, Icerya aegyptiaca

Lac cultivation is generally carried out on a limited number of indigenous tree species found scattered in forests and cultivable land. Recent studies show that Flemingia semialata Roxb. (Leguminosae) is an excellent bushy host plant for the cultivation of the kusmi strain of lac insects. The present investigation was carried out with the objective of getting acquainted with the different eco-friendly insecticides under laboratory evaluation against the Egyptian fluted scale, Icerya aegyptica. Maximum nymphal mortality of 81.67 per cent and 78.34 per cent was observed at 0.2 per cent Pestoguard and Neemazal after 12 days of treatment, which is statistically at par with each other. 51.67 per cent mortality was achieved after 12 days of treatment at 1 per cent Uro-insecticide and Green mealikil, which is a microbial pesticide that is also effective against the Egyptian fluted scale, Icerya aegyptiaca. The mortality percentage was low after 1, 3 days and seven days, but it gradually increased after 12 days of treatment, obtaining 48.34% mortality, which is statistically at par with Uro-insecticide. All the eco-friendly insecticides used in the present experiment (Uroinsecticides, Pestoguard, green mealikil and neemazal) are effective in controlling the population of Icerya aegyptiaca. Unlike Malathion and Imidacloprid, they don’t have a quick knockdown effect; however, the mortality due to exposure significantly increases as there is an increase in days or duration of application, and they are safe for the lac insect.

Praxelis cleggiae (Compositae: Eupatorieae: Praxeliinae), a new species from the granite inselbergs in the east of the Departamento de Santa Cruz, Bolivia

SummaryPraxelis cleggiae (Compositae: Eupatorieae: Praxeliinae) is described and illustrated from the granite inselbergs (upon which it is most common), and on road crown chippings on old roads, in the Departamento de Santa Cruz, Bolivia; it has a disjunct distribution and is also found on the Serranía San Simón, in the San Ignacio Schist belt, Departamento de Beni. Material of this species was mostly determined as P. insignis (Malme) R.M.King &amp; H.Rob., a Brazilian species, described from the metamorphosed sandstone of what is now the Parque Nacional da Chapada dos Guimarães in Mato Grosso State. The two species have some superficial resemblance, but differ significantly in plant size, internode length (relative to leaf length), branching, leaf shape and size, phyllaries, achenes and pappus. The total number of species of Praxelis recognised in Bolivia is now seven, and 20 species in the genus worldwide.

Can a naturally depauperate Ephemeroptera, Plectoptera and Trichoptera (EPT) fauna track river degradation in south-western Australia?

Freshwater aquatic ecosystems are threatened globally. Biological monitoring is required to deliver rapid and replicable assessment of changes in habitat quality. The Ephemeroptera, Plectoptera, Trichoptera (EPT) index is a globally recognised rapid bioassessment that measures taxa richness of three insect orders whose larvae are considered sensitive to freshwater habitat degradation. South-western Australia contains threatened freshwater ecosystems but has depauperate EPT fauna and high endemism, potentially reducing the capacity of the EPT index to track degradation. This study investigated if EPT species richness, composition or individual species tracked physical or chemical river degradation in three catchments in south-western Australia. We sampled EPT fauna and measured water chemistry, erosion, sedimentation, riparian vegetation cover and instream habitat at 98 sites in the winters of 2007 and 2023. We found 35 EPT taxa across the study area with a median number of species per site of two. EPT species richness had weak positive associations with a composite water quality index and dissolved oxygen and weak negative associations with electrical conductivity and total nitrogen. No association was found between physical and fringing zone degradation measures and EPT species richness. EPT community structure generally did not distinguish between sites with high or low degradation levels. The presence of the mayfly Nyungara bunni tracked salinity, dissolved oxygen and nitrogen levels, but its usefulness as a bioindicator could be limited by its restricted range. This study suggests that the EPT index would need modification or combination with other indices to be a useful rapid bioassessment in south-western Australia.

Data coverage, biases, and trends in a global citizen‐science resource for monitoring avian diversity

AbstractAimUnderstanding and addressing the global biodiversity crisis requires ecological information compiled continuously from across the globe. Data from citizen science initiatives are useful for quantifying species' ecological niches and geographical distributions but can be difficult to apply towards biodiversity monitoring. The presence of fixed geographical locations reduces the opportunistic nature of citizen science data, allowing for more reliable and nuanced trend estimation. The eBird citizen‐science program contains predefined locations whose bird assemblages are sampled across years (‘hotspots’). For hotspots to function as a biodiversity monitoring resource, issues related to data coverage, biases, and trends need to be addressed.LocationGlobal.MethodsWe estimated the survey completeness of species richness at 300,500 eBird hotspots during 2002–2022. We documented sampling biases at eBird hotspot and non‐hotspot locations during 2022 based on protection status, temperature, precipitation, and landcover.ResultsA total of 10,410 bird species (ca. 96.9% of total) were recorded at hotspots. The number of hotspots, checklists, and participants and the quality of species richness estimates increased worldwide with the Nearctic containing the strongest and most consistent trends. Compared to non‐hotspots, hotspots oversampled areas with higher protection status. Hotspots and non‐hotspots oversampled warmer and wetter locations in the Antarctic, Nearctic, and Palearctic, and cooler locations in the Afrotropics, Australasia, and the Neotropics. Hotspots and especially non‐hotspots oversampled urban areas. Hotspots and non‐hotspots undersampled shrublands in Australasia. Hotspots and especially non‐hotspots undersampled forests in the Afrotropics, Indomalaya, Neotropics, and Oceania.Main ConclusionsHotspots have captured a large component of the world's avian diversity but have done so inconsistently across space and time. Data quantity and quality are increasing in many regions, but the presence of regionally specific sampling biases and spatial uncertainty in hotspot locations should be addressed when applying the data.