Among the four palynozones established for the Pennsylvanian–Permian sedimentary succession of the Paraná Basin, two are related to the glacial (AcZ: Ahrensisporites cristatus and CmZ: Crucisaccites monoletus zones) and one is related to the following terminal glacial phase (VcZ: Vittatina costabilis Zone) in this portion of Gondwana. The AcZ and the CmZ are typically recognized within the basal and middle portions of the Itararé Group and the Aquidauana Formation, while the VcZ corresponds to the upper portions of both units. In this paper we present a palynostratigraphic analysis of all occurrences of these palynozones from 66 boreholes and 29 outcrops. Two paleogeographic maps are depicted showing the distribution of all sections where the palyonozones related to glacial and terminal glacial sections occur within the Itararé Group and the Aquidauana Formation. Glacial palynozones (AcZ and CmZ) are recognized from outcrops and boreholes from Santa Catarina, Paraná, São Paulo, Mato Grosso do Sul, Mato Grosso and Goiás states, encompassing the thickest portion of the Itararé Group and the Aquidauana Formation. The terminal glacial palynozone (VcZ) was recognized from several boreholes and outcrops, well distributed throughout the basin, from the Rio Grande do Sul, Santa Catarina, Paraná, São Paulo and Mato Grosso do Sul states. Reworked palynomorphs from Devonian and Mississippian strata found together with indigenous species in assemblages assigned to the glacial palynozones (AcZ and CmZ) are used herein as a tool for interpretation of the source area and as indicators of transport direction. As observed elsewhere in Gondwana, palynozones related to the glacial and terminal glacial phases present dominance of spores and monosaccate pollen grains, whereas those linked to the latter phase record the income and the quantitative increase of certain species indicative of progressive climate amelioration. Further sedimentary and stratigraphical aspects are also discussed.