Gingival Carcinoma Research Articles

肉腫様変化を示した再発下顎歯肉癌の１例

肉腫様変化を示した再発下顎歯肉癌の１例

Clinical, radiological and histopathological studies of squamous cell carcinoma of lower gingiva

In order to improve the treatment results of the lower gingival carcinoma, the treatment results were analysed based on the X-ray views, histopathological findings and mode of treatment. A series of 152 cases of primary lower gingival carcinomas treated at the First Department of Oral and Maxillofacial Surgery, Tokyo Medical and Dental University, during the 25 years between 1964 and 1988 was surveyed. The lesions with the invasive type of bone resorption on the X-ray picture often had unclear tumor margin histopathologically and those of the pressure type had a smooth front, with a small number of exceptions. The five-year cumulative survival rate was 76.7% for the pressure type, 64.6% for those without bone resorption, 53.9% for the invasive type. The deeper the resorption was, the higher the death rate was in the invasive type. Grade II of WHO classification and Grade 3 of the mode of invasion had the least recurrence rate and the least frequency of delayed cervical metastases. Grade 4D showed the highest recurrence rate and Grade 4C a high frequency of cervical metastases. Five-year cumulative survival rate was 77.8% for the cases treated by surgery, 65.6% for those by the combination of surgery and radiation, 57.1% for those by chemotherapy and 29.5% for those by radiation.

Squamous Cell Carcinoma of the Gingiva Presenting as an Endoperiodontic Lesion

An unusual case of gingival carcinoma, initially presenting as an endoperiodontic lesion without clinical evidence of malignancy, is presented. It is important to consider carcinoma of the gingiva in cases of an endoperiodontic lesion unresponsive to therapy, and to investigate further when bone resorption occurs around one tooth. Submission of all dental tissue removed during oral surgical procedures for pathologic analysis may be beneficial.

Cyclosporin-induced gingival carcinoma

Cyclosporin-induced gingival carcinoma

ＤＭＢＡ誘発ハムスター歯肉癌の顎骨浸潤に関する電顕的研究

Bone invasion of the DMBA-induced gingival carcinoma in hamster was studied with electron microscope.In the early phase of bone invasion, light microscopic observation showed the presence of muscle and/or connective tissue between the tumor and the bone on which the proliferation of osteoclasts often occurred. Electron microscopic observation revealed the development of active ruffled border of these osteoclasts. However, in the later phase, the tumor invasion near the bone surface led to the disappearance of these osteoclasts.Direct contact of the tumor with bone was observed with the electron microscope in one case among 23 animals. Tumor cells attached to the bone with half desmosome and invaded osteocyte lacunae and bone canaliculi. But there was no development of cell organelles related to bone resorption in the cytoplasm of the tumor cells, and lamina limitans was clearly observed in the bone surface facing to the tumor. Thus, the ultrastructural evidence that the tumor cells themselves directly resorb bone matrix was not obtained, though the tumor cells attaching directly to the bone may have some functions in bone resorption.

A transforming Kirsten ras oncogene in an oral squamous carcinoma

This investigation has employed the NIH 3T3 cell transfection assay in an effort to detect transforming genes in DNA from squamous carcinomas of the head and neck. Of 11 tumor DNAs tested, 1 DNA sample from a gingival squamous carcinoma was able to produce primary and secondary transformants containing the human K-ras oncogene. This is the first report of an activated ras oncogene derived from a carcinoma of the head and neck. Head and neck cancers may possess activated ras oncogenes more often than is indicated by this study because of the relative inefficiency of transfection assays in detecting large transforming genes such as K-ras.

辺縁性歯周炎との診断のもとにヒドロキシアパタイトか粒が填塞された下顎歯肉癌の１例

辺縁性歯周炎との診断のもとにヒドロキシアパタイトか粒が填塞された下顎歯肉癌の１例

４‐ｎｉｔｒｏｑｕｉｎｏｌｉｎｅ‐１ ｏｘｉｄｅ（４ＮＱＯ）飲料水投与法による実験的歯肉癌の発生 とくに付着上皮発癌とヒト慢性歯周炎型歯肉癌の組織発生との関連について

The main purpose of this study was to experimentally classify the histogenesis and malignant course of gingival carcinoma, showing its relationship to the clinical progress of chronic periodontitis.The experiment was performed using an oral cancer model induced by the oral administration of 4 NQO solution. Seventy S-D rats were given administered with a 0.001% 4 NQO solution for 34 weeks. Ten rats were prepared as controls. Fifty-seven rats were killed with ether at intervals between 30 and 75 weeks after the beginning of administration of 4 NQO, and the gingiva was histologically examined at the first and second molars, and at the retromolar area in both maxilla and mandible.The incidence of gingival carcinoma was relatively high; 80% between 60-64 weeks. All carcinomas induced were of the squamous cell type. Carcinogenesis in the gingiva was observed in the junctional and differentiation and gingival epithelium. Histogenesis in the junctional epithelium was also discernable parthy as a result of malignant processes in the inner epithelium; particularly by malignant changes localized in the junctional epithelium, slight dysplasia of the gingival oral epithelium, and the maintenance of on obvious border between junctional and gingival epithelium. Malignant changes in the junctional epithelium, combined with extension of epithelium in to the cementum, exfoliation of epithelium and retraction away from the enamel by dysplastic changes all resulted in the formation of a gingival pocket. Finally, invasion of the periodontium by cancer nests resulted in the so-called “floating tooth”Carcinogenesis in the junctional epithelium may indicate the histogenesis of human gingival carcinoma, whilst appearing clinically to follow the course of chronic periodontitis. It is considered that formation of gingival pockets by malignant changes of junctional epithelium further indicate the clinical charactristics of gingival carcinoma of this type.Malignant processes in junctional epithelium by replacement of epithelium by atypical cells indicates “epithelial dysplasia-carcinoma in situ-early invasive carcinoma”. In the gingival epithelium, the malignant processes showed “epithelial dysplasia-early invasive carcinoma” by atypical downward proliferation of epithelium.

腸骨による下顎骨再建後にハイドロオキシアパタイトか粒を用いて絶対的歯槽堤形成を行った１症例

A new method of ridge extension using hydroxyapatite particles is described. This method was applied to a patient who received transplanted iliac bone after the resection of gingival carcinoma. Ridge extension using autogenous bone has the disadvantage of possible resorption neccessitating further surgery. Hydroxyapatite however, is not absorbed and was therefore selected as being suitable for ridge extension. There are various types of hydroxyapatite ie porous particular, dense particular, porous block and dense block types.Block type is most suitable for alveolar process formation, but perforation is a frequent problem. Porous particular type resists infection due to of discharging particle individually. Alveolar process formation is difficult, and suitable methods for the application of hydroxyapatite particles to the resected and reconstructed jaws have yet to be found.We report a new method of ridge extension using hydroxyapatite particles, obtaining satisfactory results even in a unfavorable cases.

Basic studies on CaO-P2O5-MgO-SiO2-CaF system glass ceramics. 2. Ultrastructural study on interface between culture cells and glass ceramics

The aim of this study was to determine biocompatibility of glass ceramics and adhesion of cultured cells to glass ceramics. Four established cultured cell lines, human fibrosarcoma cells (HT-1080), human gingival carcinoma cells (Ca9-22), human osteosarcoma cells (NY) and mouse osteoblasts (MC3T3-E1), were used. For phase-contrast and electron microscopic observation they were cultured on substrates of glass ceramics or polystyrene coverslips as a control. The results obtained were as follows. Glass ceramics caused neither cellular degeneration nor death, as revealed by phase-contrast microscopy. By transmission electron microscopy an amorphous structure similar to the basal lamina was observed at the interface between the substrates and Ca9-22, and between glass ceramics and NY. A similar structure sometimes existed between the substrates and MC3T3-E1. On the other hand HT-1080 showed no such structure. The findings suggest that the biocompatibility of glass ceramics was satisfactory. Furthermore, from the clinical point of view it seems to be possible to close the material-tissue interface with epithelial, fibrocytic and osteocytic cells.

術中に急性ＤＩＣを生じた下顎歯肉癌患者の治療経験

A case of disseminated intravascular coagulation syndrome (DIC), which was induced during surgical operation and cured by the prompt treatment, is reported. The patient was a 52-year-old female with gingival carcinoma at the mandible (T2N0M0). Radiation therapy with 60Co (total dose of 30 Gy) and chemotherapy (100 mg pepleomycin. and 60 g tegaful in total dose) were carried out preoperatively. Tumor excision with segmental mandibulectomy, immediate reconstruction of the mandible by use of A-O osteosynthetic plate and radical neck dissection were performed.Close to the end of the surgical procedure, hemorrhagic diathesis arose. Data of laboratory examination immediately after the operation (7.7×104/mm3 of platelets, 33% of prothrombin time and 43 mg/dl of fibrinogen) suggested acute DIC.Heparin, FOY (gabexate mesilate), and FFP were immediatly administrated and then the hemorrhagic diathesis disappeared. Seven weeks were required to cure the chronic DIC completely.At present, the patient is free from recurrence of both DIC and carcinoma.

Inhibition of human gingival carcinoma cell growth by prostaglandins

The inhibitory effects of various prostaglandins (PGA 2, PGD 2 and PGE 2) on the growth of human gingival carcinoma cell line Ca 9–22 and human cervical carcinoma cell line HeLa were studied in vitro. 1. 1. PGA 2 and PGD 2 significantly depressed Ca 9–22 cell proliferation in dose and time related fashion. 2. 2. The inhibitory rate by PGs for Ca 9–22 cells exceeded than HeLa-cells. 3. 3. IC 50 value of PGD 2 on growth of Ca 97ndash;22 was calculated to be 4 μg/ml.

１３年後に局所再発と脳内転移をきたした腺様嚢胞癌の１例

A 73-year-old female was admitted to our hospital with a complaint of left temporal swelling. She had a history of gingival adenoid cystic carcinoma in 1967, and was operated for left maxillary cancer including a partial resection with left selective lymphnode resection. Radium needle application was done against recurrence at that time. After these treatments, she was cancer free until November, 1980.Pathological examination showed recurrence in the gingiva around maxillary defect. CT scan revealed that the tumor proliferated from the maxilla to the left temporal region. It was suspected that it has mestasted to brain. Chemoserapy using adriamycin and mitomycin C, and x-ray irradiation therapy had been done effectively. The tumor in the temporal region decreased in size after these therapies.We reported a case of adenoid cystic carcinoma that recurred 13 years after the first treatment. The pertinent literature was reviewed and the clinical procedure discussed.

下顎歯肉癌の術前治療後の骨組織新生状況

The effect of the pre-operative therapy on the formation of new bone was examined in ten cases of gingival carcinoma of the mandible. All of the cases was treated with radiotherapy and/or chemotherapy before surgical operation. The effect of the pre-operative therapy was evaluated on the histopathological basis of classification by Oboshi and Shimosato. As a results, the formation of new bone was notably observed in four of ten cases. These four cases were G.III or IV in Oboshi and Shimosato's classification. Such active formation of new bone progressed along the periosteum and aponeurosis.

化学療法が奏効したと思われる高齢者歯肉がんの２例

Two aged patients, i.e., a 75-year-old patient with the well differentiated squamous cell carcinoma originating in the gingiva and an 80-year-old patient with the verrucous carcinoma, showed excellent responses to daily administration of a small dose (2.5mg) of Peplomycin.In the patient with the lower gingival carcinoma, the tumor no longer existed when the patient returned one year after discharge due to a total dose of 80mg of Peplomycin, 43, 500mg of Futraful suppository, 3, 400 rad of external 60Co irradiation, 750mg daily of Futraful suppository and 3.0g daily of Krestin over 1 year after discharge. In the other patient with the upper gingival carcinoma, administering of a total dose of 22.5mg of Peplomycin alone regressed the tumor, which was resected under local anesthesia.In both patients, the favorable general condition during the treatment including immunity was thought to be greatly contributory to the excellent responses.

術後ＤＩＣの合併をみた下がく歯肉がんの１例

A case of DIC occurred following neck dissection and resection for gingival carcinoma of the mandible was presented. The treatment was successfully performed with continuous heparin drip, and concomitant respiratory distress was treated with artificial ventilation and administration of diuretics. The importance of careful observation for various symptoms in DIC was emphasized.

ヒト歯肉がんの無血清培養株の樹立と，その応用に関する研究 ２ ヒト歯肉がん由来無血清培養株（Ｃａ９‐２２ＳＦ）に対する成長因子の影響に関する研究

ヒト歯肉がんの無血清培養株の樹立と，その応用に関する研究 ２ ヒト歯肉がん由来無血清培養株（Ｃａ９‐２２ＳＦ）に対する成長因子の影響に関する研究

ヒト歯肉がんの無血清培養株の樹立と，その応用に関する研究 １ ヒト歯肉がんの無血清培養株（Ｃａ９‐２２ＳＦ）の樹立

ヒト歯肉がんの無血清培養株の樹立と，その応用に関する研究 １ ヒト歯肉がんの無血清培養株（Ｃａ９‐２２ＳＦ）の樹立

Experimental production of gingival carcinomas by local application of 9,10-dimethyl-1,2-benzanthracene (author's transl)

Experimental production of gingival carcinomas by local application of 9,10-dimethyl-1,2-benzanthracene (author's transl)

Evaluation of treatment of advanced primary and secondary gingival carcinoma.

Evaluation of treatment of advanced primary and secondary gingival carcinoma.