Gillnets are widely used in research and commercial fishery activities. As passive gear, gillnets can be selective and dependent on the diel migration of fish. In areas with limited littoral extent, inshore–offshore migration may cause bias in the gillnet catch. Our hypothesis was that some factors, such as gillnet saturation, fish depletion, or chemical cues, could be the cause of the bias. We used a total of 66 CEN gillnets deployed at Římov Reservoir parallel to the shore at different positions of littoral-pelagic gradient. Individual fish direction was recorded from inshore, offshore, or unknown direction (i.e., entangled fish). A total of 5791 fishes from nine different species were caught. For most fish, it was possible to determine their directivity, and most fish were captured in littoral or first pelagic gillnets. Shallower and deeper benthic gillnets differed in their bleak (Alburnus alburnus) catch. No significant differences were found between fish directions. At the species level, only asp (Leuciscus aspius) and ruffe (Gymnocephalus cernua) showed differences between the captured directions in one case. The results support the assumption that gillnet capture is a random process that to a great extent is connected to random local movements. This is good news for fish monitoring projects. Sampling catch is likely to reflect true changes in the fish community, and not the effects of the deployment of the sampling gear. The experiment also showed that fish directivity statistics can be used for investigation of fish behavior and gear performance.