Aims: Screening of tomato germplasm against fusarium wilt and tomato leaf curl virus diseases in naturally ventilated polyhouse. Place and Duration of Study: The experiment was conducted during rabi 2020-2021 under naturally ventilated poly house, which located at Center of Excellence, Dr. Y.S.R. Horticultural University, Venkataramannnagudem. Methodology: Total 21 tomato genotypes which included 18 germplasm lines and 3 commercial cultivars as a check (Arkavikas, Polyana and BSS 1006) collected from the HRS, Venkataramannagudem which were screened against major diseases of tomato under naturally ventilated polyhouse and to find out resistance source. Results: Among the 21 genotypes screened against tomato leaf curl virus lowest per cent disease incidence was recorded in VRSL (Venkataramannagudem Solanum lycopersicum) -114 (5.88 %), VRSL-134 (6.54 %) and VRSL-136 (6.86 %). Highest per cent disease incidence was recorded in Arka vikas (17.87 %). Among the 21 genotypes screened lowest per cent disease incidence was recorded in VRSL -40 and VRSL-134 (9.79 %). Highest per cent disease incidence was recorded in A.vikas (32.28 %).