Fruit Germination Research Articles

Impact of different PGR on seed germination of dragon fruit (Hylocereus undatus) under Chhattisgarh plain

Impact of different PGR on seed germination of dragon fruit (Hylocereus undatus) under Chhattisgarh plain

Optimizing germination: comparative assessment of various growth media on dragon fruit germination and early growth

Dragon fruit (Selenicereus undatus), known for its captivating appearance and remarkable nutritional profile, has garnered considerable attention in recent years. Despite its popularity, there's a dearth of research on optimal conditions for seed germination and early growth stages such as seedling shoot length, which are crucial for optimal crop yield. This study aims to bridge this gap by evaluating various growing media's performance on dragon fruit germination and early growth stages. Dragon fruit seeds were obtained from local markets in Pakistan and evaluated in five different growing media: cocopeat, peat moss, sand, vermiculite, and compost. Germination parameters were observed for 45 days, including seed germination percentage, mean germination time, and mean daily germination percentage, among others while early growth was monitored for 240 days. Statistical analysis was conducted using ANOVA and Tukey’s HSD test. Significant differences were found among the growing media regarding germination percentage, mean germination time, and mean daily germination. Vermiculite exhibited the highest germination rate (93.33%), while compost showed the least (70%). Peat moss and sand media facilitated rapid germination, while compost showed slower rates. Stem length was significantly influenced by the growth media, with compost supporting the longest stems. Vermiculite emerged as the most effective medium for dragon fruit seed germination, while compost showed slower but steady growth. These findings provide valuable insights for optimizing dragon fruit cultivation, aiding commercial growers and enthusiasts in achieving higher yields and quality. Further research could explore additional factors influencing dragon fruit growth and development.

Effectiveness of Nanoformulated Plant Growth Regulators in the Production and Propagation of High-value Crops and Ornamentals

Among the major classes of plant hormones, auxins, gibberellins, and cytokinins are widely used for plant propagation. These hormones influence the physiological and developmental processes of plants such as root initiation, apical dominance, seed germination, leaf expansion, and development of buds, flowers, and fruits. Its exogenous application was found to significantly improve the growth of several important plants. This study aimed to determine the effectiveness of plant growth regulators (PGRs) derived from naturally occurring plant growth-promoting bacteria (PGPBs) in the production and propagation of selected crops. In this study, indole- 3-acetic acid (IAA) and gibberellic acid (GA) were extracted from naturally occurring PGPB Bacillus sp. The extracted hormone was nanoformulated for controlled release and increased uptake in plants. The nanoformulated hormones were applied in the propagation of coffee and the production of eggplant and ornamentals. Results showed that the application of nanoformulated IAA (Nano-IAA) significantly increased the survival rate of coffee compared to the commercially available auxin. Nano-IAA increased the germination rate of eggplant in acidic soil and root emergence of Bougainvillea over the negative control (water) but was comparable to that of the commercially available auxin. The nanoformulated GAs and commercially available

Fruit wings accelerate germination in Anacyclus clavatus.

The lateral membranous expansions of fruits, commonly referred to as wings, have long been theorized to serve only dispersal functions. Alternatively, because winged fruits typically have earlier seed germination than unwinged fruits, we hypothesized that wings could increase the contact surface with water, ultimately triggering earlier germination. We investigated this alternative hypothesis by exploring the potential role of fruit wings on germination in the heterocarpic species Anacyclus clavatus (Desf.) Pers. (Asteraceae), which produces both winged and unwinged fruits. First, we measured the speed and degree of water absorption in winged and unwinged fruits. Second, we investigated the effects of wings on germination performance, by either reducing wing size or by preventing water absorption by sealing wings with wax. Next, we tested the influence of water availability on the germination performance of winged and unwinged fruits by reducing the water potential. Winged fruits absorbed more water at a faster rate than unwinged fruits. The sealing of wings delayed germination, whereas germination time was not significantly altered by wing cutting. The restriction of water availability by decreasing water potential significantly delayed seed germination of unwinged fruits, whereas winged fruits remained unaffected. Altogether, our results support the effect of wings on germination and cast doubt on the unique role of wings in dispersal. Whether or not wings contribute to dispersal, we propose that they also improve seed germination and seedling establishment by facilitating water absorption after the release from their mother plants.

Effect of passage through the common palm civet gut on seed germination of fleshy fruits in Pachmarhi Wildlife Sanctuary, central India

Frugivorous carnivores like civets consume fruits of many species and play an important role in their seed dispersal by virtue of depositing their scats farther away from the parent tree. This study was conducted to understand the role of the common palm civet in affecting the seed germination rate of three plant species (i.e. Syzygium cumini, Phoenix sylvestris and Ficus racemosa) in a tropical moist deciduous forest. We collected scats of common palm civets in Pachmarhi Wildlife Sanctuary, central India, and monitored the germination rate of these species for 90 days. Further, to understand the patterns of seed selection (if there are any), for seeds of a specific size, we compared the length, width, and mass of the civet‐ingested seeds with those of the control seeds. We had to discard the analyses on F. racemosa as none of its seeds in any treatment group germinated even after 50 days. Our results show a differential impact of gut passage: civet‐ingested P. sylvestris seeds experienced a higher germination percentage compared to the control seeds, while gut passage did not alter the seed germination of S. cumini. However, seed germination speed (proportion of seeds germinated per day) for both species, P. sylvestris and S. cumini, was faster for civet‐ingested seeds compared to their respective controls. Our findings also showed that civets preferred seeds that were shorter in length and width, and weighed less, in the case of P. sylvestris; however, no such statistically significant difference was found for seed selection in the case of S. cumini. Thus, our study shows that civets act as a legitimate seed disperser of plant species and exert a differential impact in altering the germination rate.

Photoperiod and temperature on seed germination of passion fruit 'SCS437 Catarina'

The passion fruit (Passiflora edulis Sims.) propagates by sexual reproduction and presents slow and uneven seed germination. This work has the goal of identifying a temperature and photoperiod that provide greater germination of the cultivar SCS 437 Catarina. We used an entirely randomized experimental design in a 2 x 3 + 1 factorial: two temperatures (alternating between 20/30 ºC and constant 25 ºC) and three photoperiods (8/16 – 8 hours of light and 16 hours of darkness; 12/12 – 12 hours of light and 12 hours of darkness; and absence of light), and for alternating temperature, an absence of light treatment was added, being classified as neutral photoblastic, and at a constant temperature of 25 ºC made germination unfeasible, while the 20/30 ºC alternating temperatures provided an average germination of 75%. The seeds showed an indifferent behavior to light, and the temperature alternated with 12/12 h photoperiod was the ideal condition to promote a higher percentage of germination.

Germinação in vitro de sementes de maracujá em função da remoção do tegumento e meios de cultura

ABSTRACT The presence of salts in the culture medium and the addition of phytohormones, necessary to break seed dormancy in the in vitro germination of passion fruit, can induce critical values of osmotic potential. This can change the membrane permeability of seeds, affecting the hydration rate, enzyme release, ion transport, pH, and inhibitor values, which may decrease germination percentage. The aim of this study was to evaluate different types of culture media, composed of other substances, combined with mechanical techniques of tegument removal to determine the most appropriate culture medium for the in vitro germination of Passiflora edulis (Sims flavicarpa Deg.) seeds obtained by open pollination. The experimental design was completely randomized in a 10 × 3 factorial scheme (10 culture media × three types of seed-intact, scarified, and cut), with eight replicates and five seeds per plot. Scarified seeds cultivated in a medium composed of a commercial substrate and gibberellic acid presented a higher percentage of germination than intact or cut seeds cultivated in different concentrations of Murashige & Skoog (MS) medium. Scarification is a satisfactory method for breaking the dormancy of passion fruit seeds, and commercial substrates Bioflora® enriched with gibberellic acid may replace in vitro germination.

Effects of cadmium stress on fruits germination and growth of two herbage species.

Cadmium (Cd) pollution is a global environmental problem. It is of great significance to find a kind of pasture that can grow normally in a cadmium environment, especially in the Tibetan Plateau. We studied the fruit germination and fruit growth of Elymus sinsubmuticus S.L. Chen and Elymus tangutorum (Nevski), native plants of the Tibetan Plateau, in different cadmium environments. The results showed that with increased cadmium stress, the fruit germination rate, final germination rate, fruit-vigor, average germination time, and germination-speed index for the two grass species gradually decreased, and the 50% germination time for the seed gradually increased. Root length, biomass, and the number of leaves decreased in both species. We quantified the fruit germination and growth of plants in the cadmium environment and found that E. sinosubmuticus S.L. Chen had better fruit germination and fruit growth, and it had the development potential of cadmium pollution control.

Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex.

The rapid production and numerous applications of nanomaterials warrant the necessity and importance of examining nanoparticles in terms to their environmental and biological effects and implications. In this study, the effects of a water-soluble hyper-harmonized hydroxyl-modified fullerene (3HFWC) on cherry tomato seed germination, seedlings growth, physiological response and fruiting was evaluated. Changes in the photosynthetic pigments content, oxidative stress assessment, and aquaporin genes expression in cherry tomato plants were studied after during short- and long-term continuous exposure to 3HFWC nanosubstance (200 mg/L). Increased levels of photosynthetic pigments in leaves, lycopene in fruits, decreased levels of hydrogen peroxide content, activation of cellular antioxidant enzymes such as superoxide dismutase, catalase and peroxidase and increased aquaporin gene expression (PIP1;3, PIP1;5 and PIP2;4) were observed in 3HFWC nanosubstance-exposed plants in comparison to control, untreated cherry tomato plants. The 3HFWC nanosubstance showed positive effects on cherry tomato seed germination, plantlet growth and lycopene content in fruits and may be considered as a promising nanofertilizer.

The Foraging Activity and Pollination Services of Three Stingless Bee Species to Enhance Fruit Quality and Quantity of Okra (Abelmoschus Esculentus L.)

Insect pollination has been reported to have a better yield quality and quantity than artificial pollination. Wild bees, including stingless bees contribute to effective and efficient pollination on okra (Abelmoschus esculentus L.: Malvaceae). However, previously no report about okra pollination by native Indonesian stingless bees, which can be potential as pollinators. Therefore, this research aims to compare foraging activities of three species of stingless bees (Heterotrigona itama, Tetragonula laeviceps, and T. clypearis) on okra plants and fruits quality and quantity parameters among four pollination treatments (three stingless bee species and without pollinator). Four cages with 50 okra plants were used for pollination treatments. The foraging observation of different stingless bee species on okra was conducted in the cages, and the okra yield from each treatment was compared. Our results showed the foraging activities of three stingless bee species were higher in the morning than those in the afternoon. In the morning, the number of visits of T. clypearis and T. laeviceps were negatively correlated with visit duration. The current study also showed that size and weight of pod, the number of seeds, and the percentage of fruit set of okra were enhanced by pollination of three stingless bee species. Unfortunately, the pollination of stingless bee species did not affect the seed germination and flavonoid content of fruits. The native of Indonesian stingless bees are good pollinators to improve pod quality and quantity.

The Effect of Temperature and Moisture on Colonization of Apple Fruit and Branches by Botryosphaeria dothidea.

Botryosphaeria dothidea causes severe disease of apple trees in China. The process of conidium germination, colonization, and infection of apple fruit and branches was examined on 'Fuji' apple and the effect of temperature, surface wetness and relative humidity (RH), and host surface washates on these processes was studied in controlled environments. Initial germ tube development and hyphal growth resulted in the colonization of the host surface without forming an infection structure. Hyphae expanded radially across the host surface and, after entering lenticels, developed into a dense mycelium mass or differentiated pseudoparenchyma. Hyphae from the bottom of the pseudoparenchyma either directly penetrated the lenticel surface intercellularly through the cell layer, or formed an undifferentiated hypha that invaded the lenticel through cracks formed during the lenticel development. Conidial germination and hyphal colonization occurred at 10 to 40°C, with an optimum of approximately 28°C. Conidial germination required an RH > 95% or surface wetness but, for hyphal colonization, an RH > 90% was sufficient. Conidia germinated and formed germ tubes within 1 h under optimum conditions. However, the pathogen required a longer period at RH > 90% or surface wetness for hyphae to colonize and form pseudoparenchyma or dense mycelia on the host surface. Hyphal colonization is a crucial stage for infection of apple tissues by B. dothidea.

Characterization of Okra Species, Their Hybrids and Crossability Relationships among Abelmoschus Species of the Western Ghats Region

Wild relatives of okra represent a good source of variation for breeding programs, particularly for traits related to biotic and abiotic stresses and fruit quality. However, wild species remain largely unexploited for okra breeding. The cultivated okra accession Abelmoschus esculentus cv. ‘Arka Anamika’ was crossed with three wild accessions: Abelmoschus manihot, Abelmoschus ficulens and Abelmoschus moschatus. The crossability was estimated based on fruit set, seed set and germination rate. The results of cross compatibility studies revealed that the crosses are compatible only when cultivated A. esculentus is used as a female, and reciprocal crosses were not successful in any of the three wild species. Higher fruit set (87.2%) and seed germination (92%) were obtained from the crosses ‘Arka Anamika’ × Abelmoschus manihot. The interspecific F1s exhibited normal growth, flowering and fruit formation, but the hybrids were completely sterile on selfing due to abnormal meiosis. Characterization of the parents and interspecific hybrids revealed that the interspecific hybrids were generally more vigorous than the parents and displayed greater alliance towards their wild parent. The sterile F1s were further treated with 0.1% colchicine to restore fertility. The three interspecific crosses showed considerable differences in mean performance and heterosis over mid parent and better parent. The cross ‘A. Anamika’ × A. manihot displayed positive heterosis over mid parent and better parent for the yield traits, viz., fruit yield per plant, number of fruits per plant, number of primary branches per plant and plant height. The information obtained in the study on crossability relationship, phenotypic characterization and heterosis in interspecific hybrids will assist breeders in the development of prebreeding material.

Germination study in Gokhru (Pedalium murex L.) seeds

Gokhru (Pedalium murex L.) is one of the important medicinal plants propagated through seeds but no germination test has been standardized for this crop yet. Germination test is mandatory for seeds sale which is required for labelling. The fruits of Pedalium murex L. are four angled indehiscent hard pyramidal with four sharp spines and it is very difficult to extract the seeds from fruits. Hence, study was conducted to find out suitable method and optimum temperature for germination test of fruits as well as seeds of gokhru. The study was conducted by using three germination testing methods viz., Top of Paper (TP), Between Paper (BP) and Sand (S) methods and three temperatures i.e. 25, 30 and one alternating temperature of 20-30℃. The results revealed that during germination test of fruits, ‘Sand’ method showed superiority by registering maximum germination (29.67%) over ‘Between Paper’ (23.33%) and ‘Top of Paper’ (3.11%) methods. Viogur index-I (213) and Vigour Index-II (2.20) were also observed maximum in ‘Sand’ method while maximum seedling length (8.00cm) and seedling dry weight (0.075mg) were observed in ‘Between Paper’ method. Among the temperatures, maximum germination (22.78 & 36.00%), seedling length (8.00 & 9.44cm), seedling dry weight (0.142 & 0.077mg), Vigour index-I (186 &341) and Vigour index-II (2.26 & 2.79) were observed at 30℃ in fruits and seeds respectively. No germination was recorded below 20℃. Good germination was observed in seeds compared to fruits. In case of seeds, maximum germination (38.33%), seedling length (9.00cm), seedling dry weight (0.076mg), vigour index-I (329) and vigour index-II (2.77) were recorded in ‘Between Paper’ method. It is concluded that 30℃ temperature is optimum for germination test of both seed as well as fruit in Gokhru and ‘Sand’ method is best for fruit germination test while in case of germination test of seeds ‘Between Paper’ method is best.

Seedball Coating Material Formulation to Enhance Germination and Growth of Fruit and Forest Seeds

Planting using seed ball, where seeds are enclosed in materials and planted directly to the field by throwing the seed balls or using aerial planting. This technique has been considered to speed up the rehabilitation of degraded land and forest. Formulation of materials to enclose the seed is important and could be specific for different seeds. Therefore, this research aims to determine the appropriate formulation of seed balls to enhance seeds germination and their subsequent growth. The research was conducted from February-June 2021 in a greenhouse. Three formulas have been tested, i.e. 1) Formula A: clay and compost (1:1); 2) Formula B: clay, compost, and insecticide (50:50:1); 3) Formula C: clay, sawdust, bone meal, and vermicompost (8:8:2:1). The seeds used were seeds of durian (Durio zibethinus), jackfruit (Artocarpus heterophyllus), velvet apple (Diospyros blancoi), merbau (Intsia bijuga), and white teak (Gmelina arborea). The experimental design used was a completely randomized design (CRD) with 3 treatments and 3 replications. The fastest germination rate of durian, jackfruit, and merbau seeds was recorded on Formula A, while velvet apple and white teak seeds were on Formula C. The best height growth of seedlings was found using coating Formula C, i.e. 17.98 cm within 3 months after planting.

К БИОЛОГИИ OENANTHE AQUATICA (L.) POIR. (UMBELLIFLORAE)

The results of experiments on seed germination and the initial stages of ontogenesis of the hygrohelophyte Oenanthe aquatica under laboratory conditions are presented. The features of fruit germination and the initial stages of ontogenesis of Oenanthe aquatica (L.) Poir were studied. It was revealed that mature fruits of O. aquatica do not have a dormant period. Germination of both freshly harvested fruits and those subjected to various storage conditions for 4 months (in laboratory conditions, refrigerator and freezer), showed a high percentage of laboratory germination from 90.6 to 100%. At the same time, storage of fruits in the freezer and in the refrigerator led to a decrease in the lag-time and germination period (in comparison with those of freshly picked fruits and fruits stored at room temperature). It was revealed that the fruits of O. aquatica germinate without a dormant period. It was shown that the development of O. aquatica seedling under laboratory conditions lasts about 30 days. The transition of a plant to a juvenile ontogenetic state occurs when the cotyledon leaves die off and is accompanied by the appearance of all the main plant organs. The sprout is a monopodially growing rosette shoot with main and adventitious roots. The juvenile plant is also represented by a uniaxial monopodial and anisotropic rosette shoot. The transition to an immature age-related state is characterized by the preservation of the rosette form of growth and the appearance of axillary buds on the shoot. In juvenile and immature age states, plants usually hibernate. It was revealed that already at the seedling stage, the polyvariance of ontogenesis is traced according to the rates of development of plants (growing in Petri dishes and floating on the surface of the water in beakers), associated with different rates of plant transition from the seedling phase to the juvenile age state.

Removal of the epi- and mesocarp from the semi-mature fruit of Peumus boldus and not the use of gibberellic acid promotes the highest seed germination

Because of climate change, wildfire events have increased in the semi-arid ecosystems of the world. In this context, it is necessary to count with efficient tools for the rehabilitation or restoration of those systems. Plant propagation is crucial to obtain plants for rehabilitation programs, and it becomes a limiting factor in the success of those initiatives. For this, it is necessary to know how relevant factors affect seed germination in plants of interest. Peumus boldus is a dominant plant species of the Mediterranean area of Chile that has been severely affected by wildfire events. Consequently, in this investigation, the joint effect of pre-germination treatments such as epicarp and mesocarp removal with the use of gibberellic acid on the germination of semimature fruits of P. boldus was studied. The results show that only the removal of the epicarp and the mesocarp had a significant effect on seed germination, reaching a proportion of 51.4%, which is the highest value reported so far in a published study with a controlled setting for P. boldus. Therefore, to obtain a high proportion of P. boldus plantlets for use in rehabilitation programs; seeds should be collected in December and exposed to the pre-germination treatment of pericarp and mesocarp removal without using gibberellic acid in doses lower than or equal to 10 g L -1 .

Seed Germination in Blood Fruit (Haematocarpus validus (Miers.) Bakh. f. ex Forman) as Influenced by Substrate

Blood fruit is a tropical underutilized species, fruits of which have been identified as source of natural colourant. However, forests are the major source of fruits to native people. In order to conserve the natural population of this species and promote cultivation, nursery techniques need to be standardized. In the present investigation, effect of three substrates viz. vermicompost, sand and soil + vermicompost was studied on seed germination characteristics. Results revealed significant differences among the treatments. Use of vermicompost improved seed germination (69%) significantly over the other substrates used. Though most of the studied seedling growth parameters did not differ among the treatments, significantly highest seedling vigour index (5466.9) was recorded when vermicompost was used as substrate. Considering these findings, use of vermicompost is advisable for germination of blood fruit in nursery.

Seed Germination in Blood Fruit (Haematocarpus validus (Miers.) Bakh. f. ex Forman) as Influenced by Substrate

Blood fruit is a tropical underutilized species, fruits of which have been identified as source of natural colourant. However, forests are the major source of fruits to native people. In order to conserve the natural population of this species and promote cultivation, nursery techniques need to be standardized. In the present investigation, effect of three substrates viz. vermicompost, sand and soil + vermicompost was studied on seed germination characteristics. Results revealed significant differences among the treatments. Use of vermicompost improved seed germination (69%) significantly over the other substrates used. Though most of the studied seedling growth parameters did not differ among the treatments, significantly highest seedling vigour index (5466.9) was recorded when vermicompost was used as substrate. Considering these findings, use of vermicompost is advisable for germination of blood fruit in nursery.

Reliable Physical Parameters for Determining Fruit/Seed Maturity Timing of Ficus semicordata Buch. in Kumaun Region of Central Himalaya

Wild edible plants play an important role as food supplements during scarcity for local inhabitants. Ficus semicordata is commonly known as drooping fig. Plant parts are used for medicinal purposes. It is a common agroforestry tree in the Himalayan region growing from 750 to 1500 m elevation. The present study was carried out to assess the exact time of seed and fruit maturation and germination of F. semicordata in Nainital district of Kumaun Himalaya across 3 sites during two consecutive years 2017 and 2018. Fruit of F. semicordata were collected from the marked trees from all the sites till the availability of the fruits. Across all the sites the mean fruit size varied between 190.24 ± 0.46 and 395.14 ±3.56.14 mm² in Yr-1 and 135.56 ±3.69 to 516.86 ±1.26 mm² in Yr-2. Across both the sites the colour change from green to pinkish brown appears as one of the indicator of maturity. In addition to the colour fruit moisture content between 50.25±0.58% and 62.73±2.30% coincided with maximum germination (48.0±0.06% and 85± 4.61%).

Responses of Germination to Light and to Far-Red Radiation—Can they be Predicted from Diaspores Size?

This paper presents an update of a dataset of seed volumes previously released online and combines it with published data of the photoblastic response of germination of fruits or seeds (light or dark conditions), and of the effects of enhanced far-red radiation on germination. Some evidence was found to support that germination in larger diaspores might be indifferent to light or dark conditions. Similarly, germination in smaller diaspores might be inhibited by far-red radiation. However, the length, width, thickness, volume, shape, type of diaspore, or relative amplitude of volume is essentially useless to predict photoblastic responses or the effects of far-red radiation on germination of diaspores.