This study delves into the nuanced realm of medical geography, focusing on the intricate interplay between geographical factors and public health outcomes in the Samarkand and Navoi regions of Uzbekistan. Over the decades, the field of medical geography has evolved, embracing a holistic perspective that integrates ecological, socioeconomic, and accessibility elements to elucidate the complex dynamics of disease distribution and healthcare provision. Utilizing a comprehensive methodology, this research employs a combination of GIS tools, statistical analysis, and expert input to delineate medical geographical zones within the study area. By synthesizing data from diverse sources including administrative records, environmental assessments, and demographic surveys, the study constructs a detailed picture of the region's health landscape. The analysis revealed five primary medical geographical zones and a sub-zone, each characterized by distinct natural features, economic activities, and health challenges. From the bustling urban centers of Samarkand and Navoi to the remote desert expanses of Uzguduk - Tomdi North, each zone presents unique opportunities and obstacles for healthcare delivery and environmental management. Key findings highlight the disproportionate distribution of healthcare resources, with densely populated areas in the southern part of the study area benefitting from greater accessibility to medical facilities compared to remote rural regions. Moreover, environmental factors such as air pollution, water contamination, and climatic variability emerge as significant determinants of public health, exacerbating respiratory ailments, cardiovascular diseases, and other health conditions. The study underscores the importance of spatial analysis and interdisciplinary collaboration in addressing complex health issues within diverse geographical landscapes. By mapping medical geographical zones and understanding their specific health concerns, policymakers and healthcare professionals can develop targeted interventions to improve healthcare access, mitigate environmental hazards, and promote community well-being. In conclusion, this research contributes valuable insights to the field of medical geography, shedding light on the multifaceted interactions between geography, environment, and health in the Samarkand and Navoi regions of Uzbekistan. Moving forward, further research and collaborative efforts are needed to address the underlying determinants of health disparities and foster sustainable development in the region.