Geographical Affinities Research Articles

Geography and ecology structure diversification in Ziziphus Mill. (Rhamnaceae)

Understanding the processes responsible for creating and preserving biodiversity within tropical ecosystems is essential for gaining deeper insights into the history, dynamics, and resilience of these environments. We investigated the biogeography and evolution of Ziziphus Mill. (Rhamnaceae), a genus of c.50 species found in all major tropical biomes and across Africa, Asia and Australasia to evaluate how ecology and geography structures its diversification, and their associations with functional traits. We used consistency and retention indices, as well as the delta statistic to test for phylogenetic signal in ecology, geography and functional traits. We constructed a time-calibrated phylogeny using BEAST, followed by the reconstruction of biome and geographical affinities in BioGeoBEARS, and ancestral functional trait evolution using phytools and ape. At a global scale, geography structures Ziziphus’ diversification more than ecology, as evidenced by higher phylogenetic signal in retention indices and the delta statistic. Multiple ecological switches among biomes are associated with dispersal events, which structure Ziziphus’ diversification at smaller scales. Biogeographic estimation suggests that Ziziphus evolved out of closed biomes in Asia during the Oligocene. Long distance dispersal from Asia to Africa resulted in the occupation of two novel biomes, the expanding tropical grassy biome and the desertic biome. Geographic transitions across continents that retain the same biome are fewer but are demonstrated by a dispersal from the closed biome in Asia to the same ecological setting in Australia. In keeping with the younger age of the savanna biome and the Pliocene radiation of geoxylic species, the youngest species in Ziziphus are mostly found in open biomes, and the youngest habit is geoxylic. Leaf hair appears to confer advantages across biomes whilst being a synapomorphy for open and desertic clades. One of two evolutions back into closed biomes is associated with the development of the liana habit. We inferred the most complete phylogeny for Ziziphus to date. Ziziphus originated in closed biomes in Asia in the Oligocene. At a global scale, geography structures diversification more than ecology (biome preference) in Ziziphus. Biome shifts are frequently associated with long distance dispersal events. Shifts in growth form are frequently associated with biome shifts.

Occurrence of Andreimyrme substriolata (Chen, 1957) and Odontomutilla fletcheri Lelej, Terine & Girish Kumar, 2020 (Hymenoptera, Mutillidae) in India, with note on their geographical affinities

Occurrence of Andreimyrme substriolata (Chen, 1957) and Odontomutilla fletcheri Lelej, Terine & Girish Kumar, 2020 (Hymenoptera, Mutillidae) in India, with note on their geographical affinities

Perspetivas atuais da cooperação transfronteiriça da Euro-região Galiza-Norte de Portugal: os planos de investimentos conjuntos

Relations between Galicia and the north of Portugal go back a long way (to the Middle Ages), based on linguistic, cultural and historical ties over a common geographical area – the north-west of the Iberian Peninsula. More recently, in times of democracy, territorial cooperation, based on the historical ties, geographical affinities and cultural identity that connect these regions, has developed through specific programmes and projects that receive funding from different sources, including funds from the European Union (EU) under the Interreg programme and the EU’s main financial instrument for promoting economic and social cohesion between European regions, the European Regional Development Fund (ERDF). The aim of this study is to carry out a descriptive and explanatory analysis of the investment programmes of the Euroregion Galicia Norte de Portugal, identifying the evolutionary pattern of the axes of cooperation between the two regions, which aim to promote joint development and the resolution of common challenges, looking to the future of cross-border cooperation. The structure of the article is organised into three topics. The first defines the most recent evolutionary framework of relations between the two regions in the north-west of the Iberian Peninsula, the second refers to the establishment of the Galicia-Northern Portugal Euroregion and finally the priority axes of cross-border cooperation framework are analysed, with an emphasis on the strategic programs and EU funding programmes since 2007 until 2027, in a comparative perspective.

Revisiting the systematic position of the enigmatic Nicobar Bulbul (Ixos nicobariensis)

The Nicobar Bulbul Ixos nicobariensis is an endemic species found across the central Nicobar Islands whose systematic position has been debated for many decades. Its current placement in the genus Ixos is tentative and is based on morphological similarities and geographical affinities to other members of the genus. We used mitochondrial and nuclear markers to examine its phylogenetic relationship to other members of Ixos and Hypsipetes. Our results indicate that the Nicobar Bulbul is sister to members of the genus Hypsipetes, and not Ixos. We further discuss the origin of this species based on the results of our study.

Melica as an emerging model system for comparative studies in temperate Pooideae grasses.

Pooideae grasses contain some of the world's most important crop and forage species. Although much work has been conducted on understanding the genetic basis of trait diversification within a few annual Pooideae, comparative studies at the subfamily level are limited by a lack of perennial models outside 'core' Pooideae. We argue for development of the perennial non-core genus Melica as an additional model for Pooideae, and provide foundational data regarding the group's biogeography and history of character evolution. Supplementing available ITS and ndhF sequence data, we built a preliminary Bayesian-based Melica phylogeny, and used it to understand how the genus has diversified in relation to geography, climate and trait variation surveyed from various floras. We also determine biomass accumulation under controlled conditions for Melica species collected across different latitudes and compare inflorescence development across two taxa for which whole genome data are forthcoming. Our phylogenetic analyses reveal three strongly supported geographically structured Melica clades that are distinct from previously hypothesized subtribes. Despite less geographical affinity between clades, the two sister 'Ciliata' and 'Imperfecta' clades segregate from the more phylogenetically distant 'Nutans' clade in thermal climate variables and precipitation seasonality, with the 'Imperfecta' clade showing the highest levels of trait variation. Growth rates across Melica are positively correlated with latitude of origin. Variation in inflorescence morphology appears to be explained largely through differences in secondary branch distance, phyllotaxy and number of spikelets per secondary branch. The data presented here and in previous studies suggest that Melica possesses many of the necessary features to be developed as an additional model for Pooideae grasses, including a relatively fast generation time, perenniality, and interesting variation in physiology and morphology. The next step will be to generate a genome-based phylogeny and transformation tools for functional analyses.

Data driven analysis of social capital in Farmer Producer Companies

The Farmer Producer Company (FPC), a subset of the Farmer Producer Organization (FPO), is an important institutional form designed to organize farmer groups towards better coordinated farming and marketing. In the Indian context, as FPCs have emerged as new forms of members-led agribusiness, their ability to identify prevailing social ties and tap them effectively towards business growth needs to be better understood. Although social capital is studied broadly for its potential to drive organizational performance, it has been poorly researched in farmer collectives such as FPCs. The current work examines the effect of social capital on benefits and business performance at the level of member groups in FPCs. An empirical analysis was conducted in which two FPCs, which differed significantly in their mobilization strategies, farming methods, and supply chain linkages, were surveyed. Data collected from the surveys were visualized and clustering analysis was carried out using Self Organizing Maps (SOM), an unsupervised Artificial Neural Network (ANN) tool. Insights from clustering reveal the importance of pre-existing social ties, leadership, participation in group activities and the geographical affinity of groups in benefits realization and business performance of FPCs. The importance of bottom-up approaches in establishing robust supply chain linkages in emerging FPCs was keyed out through this work. The inferences through SOM, distilled strategies for FPCs' stakeholders in prioritizing interventions for member groups and in generating broader implications for policy makers accounting social capital in new institutional models.

Unravelling the genetic diversity and population structure of common walnut in the Iranian Plateau

BackgroundCommon walnut (Juglans regia L.) has a long cultivation history, given its highly valuable wood and rich nutritious nuts. The Iranian Plateau has been considered as one of the last glaciation refugia and a centre of origin and domestication for the common walnut. However, a prerequisite to conserve or utilize the genetic resources of J. regia in the plateau is a comprehensive evaluation of the genetic diversity that is conspicuously lacking. In this regard, we used 31 polymorphic simple sequence repeat (SSR) markers to delineate the genetic variation and population structure of 508 J. regia individuals among 27 populations from the Iranian Plateau.ResultsThe SSR markers expressed a high level of genetic diversity (HO = 0.438, and HE = 0.437). Genetic differentiation among the populations was moderate (FST = 0.124), and genetic variation within the populations (79%) significantly surpassed among populations (21%). The gene flow (Nm = 1.840) may have remarkably influenced the population genetic structure of J. regia, which can be attributed to anthropological activities and wind dispersal of pollen. The STRUCTURE analysis divided the 27 populations into two main clusters. Comparing the neighbor-joining and principal coordinate analysis dendrograms and the Bayesian STRUCTURE analysis revealed the general agreement between the population subdivisions and the genetic relationships among the populations. However, a few geographically close populations dispersed into different clusters. Further, the low genetic diversity of the Sulaymaniyah (SMR) population of Iraq necessitates urgent conservation by propagation and seedling management or tissue culture methods; additionally, we recommend the indispensable preservation of the Gonabad (RGR) and Arak (AKR) populations in Iran.ConclusionsThese results reflected consistent high geographical affinity of the accession across the plateau. Our findings suggest that gene flow is a driving factor influencing the genetic structure of J. regia populations, whereas ecological and geological variables did not act as strong barriers. Moreover, the data reported herein provide new insights into the population structure of J. regia germplasm, which will help conserve genetic resources for the future, hence improving walnut breeding programs’ efficiency.

Carbon (δ13C) and nitrogen (δ15N) isotope ratios reveal geographic affinity and dietary status of Ajnala skeletal remains: A forensic anthropological study.

Stable isotope analysis from bones and teeth has been widely used to estimate the likely geographic locations and dietary status of individuals whose osseous remains have been retrieved from some forensic or bio-archaeological contexts. The carbon and nitrogen stable isotope signatures can provide insights into geographic affinity and dietary habits. Ajnala skeletal remains represent a serious crime against humanity committed in past by colonial rulers and by amateur archaeologists of modern times. In present study, isotopic concentrations of carbon (δ13C) and nitrogen (δ15N) estimated from 21 mandibular molars have been used to estimate the local or non-local status of badly damaged skeletal remains retrieved from an abandoned well at Ajnala (India). The collagen samples having C/N ratio within range of 2.8-3.6 were considered as well-preserved and non-contaminated ones. The isotope concentrations of carbon and nitrogen varied from -18.7‰ to -22.9‰ and +7.6‰ to +11.7‰, with an average of -20.49 ± 1.2‰ and +9.31 ± 1.1‰, respectively. The analysis of the obtained isotope values reflected the consumption of C3/C4 mixed diet by majority of the individuals, and such type of dietary habits are mainly restricted to the reported Indo-Gangatic plain of India to which slain soldiers reportedly belonged to. These observations corroborated the previous observations about the geographic affinity and dietary status of Ajnala individuals. Though C and N isotopes are by and large not the confirmed/direct indicators of geographic origin, they can provide corroboratory information to support other observations narrowing down the dietary habits of individuals of certain specific geographical regions.

The impact of environmental and climatic variables on genetic diversity and plant functional traits of the endangered tuberous orchid (Orchis mascula L.)

Understanding how environmental factors shape patterns of genetic and phenotypic variations in a species is necessary for conservation and plant breeding. However, these factors have not yet been completely understood in tuberous orchid species used to make ‘Salep’, an important ingredient in traditional medicine and beverages in middle eastern countries and India. In many areas, increasing demand has pushed species to the brink of extinction. In this study, 198 genotypes from 18 populations of the endangered species Orchis mascula L. spanning a large-scale climatic gradient in northern Iran were used to investigate patterns of genetic diversity and plant functional traits. Populations were sampled from three land cover types (woodland, shrubland, and pastureland/grassland). Plant height, stem length, number of flowers, bulb fresh and dry weight, glucomannan, and starch concentrations showed high variation among populations and were significantly related to land cover type. In general, genetic diversity was high, particularly in those from eastern Hyrcanian; additionally, populations showed a high level of genetic differentiation (G'st = 0.35) with low gene flow (Nm = 0.46). The majority of genetic differentiation occurred within populations (49%) and land cover types (20%). The population structural analysis using the AFLP marker data in K = 4 showed a high geographical affinity for 198 O. mascula genotypes, with some genotypes having mixed ancestry. Temperature and precipitation were found to shape genetic and phenotypic variation profoundly. Significant isolation by the environment was observed, confirming the strong effect of environmental variables on phenotypic and genetic variation. Marker-trait association studies based on MLM1 and MLM2 models revealed significant associations of P-TGG + M-CTT-33 and E-AGG + M-CGT-22 markers with plant height and glucomannan content. Overall, a combination of large-scale climatic variables and land cover types significantly shaped genetic diversity and functional trait variation in O. mascula populations.

Cross-reward effects in a coalition loyalty program: The impact of a point currency devaluation

While single-brand reward programs encourage customers to remain loyal to that one brand, coalition programs encourage customers to be “promiscuous” by offering points redeemable across partner stores. Despite the benefits of this “open relationship” with customers, store managers face uncertainty as to how rewards offered by partners influence transactions at their own stores. We use a model of multi-store purchase incidence and spend to show how the value of points shared among partner stores can explain patterns in customer-level purchases across them. We also allow reward spillovers to be moderated by three measures of store affinity that characterize a coalition’s portfolio: the relative popularity, geographic distance, and overlap in product categories between each pair of stores.For the coalition studied, popularity affinity was the main determinant of the valence of cross-reward effects, both before and after the devaluation. In contrast, category and geographic affinity had a smaller and more heterogenous impact. Through the use of an event where the loyalty program uniformly devalued the entire coalition’s value of reward points, we show that cross-reward effects changed (lessened), leading to larger financial losses for the most popular stores. While we do not observe changes to the composition of the coalition’s portfolio, our results also suggest that the value of a shared reward currency may be driven by the inclusion of smaller partners.

Molecular phylogenetics reveals the evolutionary history of marine fishes (Actinopterygii) endemic to the subtropical islands of the Southwest Pacific

Remote oceanic islands of the Pacific host elevated levels of actinopterygian (ray-finned fishes) endemism. Characterizing the evolutionary histories of these endemics has provided insight into the generation and maintenance of marine biodiversity in many regions. The subtropical islands of Lord Howe, Norfolk, and Rangitāhua (Kermadec) in the Southwest Pacific are yet to be comprehensively studied. Here, we characterize the spatio-temporal diversification of marine fishes endemic to these Southwest Pacific islands by combining molecular phylogenies and the geographic distribution of species. We built Bayesian ultrametric trees based on open-access and newly generated sequences for five mitochondrial and ten nuclear loci, and using fossil data for time calibration. We present the most comprehensive phylogenies to date for marine ray-finned fish genera, comprising 34 species endemic to the islands, including the first phylogenetic placements for 11 endemics. Overall, our topologies confirm the species status of all endemics, including three undescribed taxa. Our phylogenies highlight the predominant affinity of these endemics with the Australian fish fauna (53%), followed by the East Pacific (15%), and individual cases where the closest sister taxon of our endemic is found in the Northwest Pacific and wider Indo-Pacific. Nonetheless, for a quarter of our focal endemics, their geographic affinity remains unresolved due to sampling gaps within their genera. Our divergence time estimates reveal that the majority of endemic lineages (67.6%) diverged after the emergence of Lord Howe (6.92 Ma), the oldest subtropical island in the Southwest Pacific, suggesting that these islands have promoted diversification. However, divergence ages of some endemics pre-date the emergence of the islands, suggesting they may have originated outside of these islands, or, in some cases, ages may be overestimated due to unsampled taxa. To fully understand the role of the Southwest Pacific subtropical islands as a ‘cradle’ for diversification, our study advocates for further regional surveys focused on tissue collection for DNA analysis.

Distributional patterns of Vetigastropoda (Mollusca) all over the world: a track analysis

AbstractThe distributional patterns of the species of the mollusc subclass Vetigastropoda were analysed using the panbiogeographical method of track analysis. We analysed distributional data of 434 species and 72 genera of the world obtained from specialized literature and records in 12 malacological collections. We constructed the individual tracks for the species analysed and superimposed them, applying a parsimony analysis of endemicity with progressive character elimination. We identified 26 generalized tracks, extending in the Pacific Ocean (14), Indian Ocean (six), Atlantic Ocean (four), Mediterranean Sea (one) and Atlantic–Pacific Oceans (one). The generalized tracks show the complex relationships of the mollusc biota, representing geographical and phylogenetic boundaries of taxa with diverse geographical affinities. Five panbiogeographical nodes were identified by the intersection of two or more generalized tracks. Our analysis offers an approximation for understanding the distributional patterns of vetigastropod species worldwide from the perspective of evolutionary or historical biogeography. The results obtained for the Vetigastropoda coincide completely or in part with those previously presented for other taxa. The generalized tracks support hypotheses of primary biogeographical homology that might be corroborated by evidence from other different, phylogenetically unrelated taxa.

Intellectual structure of Library and Information Science in Iberoamerica using journal co-citation analysis: a comparative study based on Scopus and Web of Science

Abstract In this study, the intellectual structure of the Iberoamerican research on Library and Information Science was visualized, from the perspective of journal citation relations. In addition, the core journals that most influence intellectual structure were identified. The study had a comparative emphasis since data from Scopus and Web of Science Core Collection were used. Journal co-citation maps were generated to identify the journal communities. Strategic diagrams with citation and betweenness centrality measures were also generated to identify core, under development, and peripheral journals. The results showed that the intellectual structure is fragmented into communities that respond to the geographic context and language affinity. Only two journals are considered core, El Profesional de la Información and Ciência da Informação. In general, there was not much difference in the comparative analyzes between the two databases. Complementary studies are necessary to show which epistemic factors influence the current fragmentation of the intellectual structure of the Library and Information Science research in the region.

Phylogeographic study using nuclear genome sequences of <i>Asip</i> to infer the origins of ventral fur color variation in the house mouse <i>Mus musculus</i>

While the house mouse (Mus musculus), widely distributed in Eurasia, is known to have substantial coat color variation between and within local populations, in both primary and secondary distribution areas, including the Japanese archipelago, the evolutionary history of the color variation is poorly understood. To address the ventral fur color variation, we quantified the lightness of museum skin specimens, and found that the southern subspecies, M. m. castaneus (CAS), has high and low lightness in dry and rainy geographic regions, respectively. The northern subspecies, M. m. musculus (MUS), has low and high levels of lightness in the high and middle latitudes of northern Eurasia, respectively. We examined sequence variation of the agouti signaling protein gene (Asip), which is known to be responsible for the ventral fur color. We performed phylogenetic analyses with 196 haplotype sequences of Asip (~180 kb) generated by phasing the whole-genome data of 98 wild mice reported previously. Network and phylogenetic tree construction revealed clustering of haplotypes representing the two subspecies, MUS and CAS. A number of subclusters with geographic affinities appeared within the subspecies clusters, in which the essential results were consistent with those reconstructed with whole mitochondrial genome data, indicating that the phased haplotype genome sequences of the nuclear genome can be a useful tool for tracing the dispersal of geographical lineages. The results of phylogeographic analysis showed that CAS mice with darker ventral fur possessed similar Asip haplotypes across the geographic distribution, suggesting that these haplotypes are major causes of the historical introduction of Asip haplotypes for darker ventral fur in mice from northern India to the peripheral areas, including the Japanese archipelago. Similarly, MUS in East Asia, which has a white abdomen, formed an Asip haplogroup with that from northern Iran, also with a white abdomen.

The Urban-Rural Divide: Spatial Inequalities and Backlash in the Investment Treaty Regime

A growing literature in international law has examined the backlash against international institutions and norms and its links with the rise of nationalist parties and populism. Some of this backlash have been said to originate with populations in small towns and rural areas socioeconomically “left-behind” by economic globalization. These developments have made salient the growing economic and political polarization between urban and non-urban areas. Nevertheless, this urban- rural divide and its implications for international law have only started to be acknowledged. Aligned with these concerns, this article adopts the urban-rural divide as a geographical scale or frame to suggest a new perspective on the investment treaty regime, its backlash and reform. Outside of the particularly virulent nature of its backlash, the regime’s context provides fertile ground for this frame: it is structured so that urban actors principally located in global or capital cities, such as multinational enterprises, global law firms, or national executive branches, make decisions about foreign investment projects that are often located and impact non-urban areas and populations. As this article contends, this context points to the regime’s potential to impact (and address) through geographical affinities the global growth of political and economic polarization between urban and nonurban areas. The impact of these urban decisions on non-urban areas has so far principally been examined through frames that emphasize impact in terms of the “environment” or “local communities” together with calls for reforms to the regime by allocating more policy space for States. An “urban-rural” frame centers additional impacts in terms of non-urban public interest, local participation, and the distribution of resources, and queries the ability of domestic policies alone to respond to them in the pursuit of socially and economic inclusive investment.

Biodiversity Assessment and Geographical Affinities of Discards in Clam Fisheries in the Atlantic–Mediterranean Transition (Northern Alboran Sea)

Biodiversity Assessment and Geographical Affinities of Discards in Clam Fisheries in the Atlantic–Mediterranean Transition (Northern Alboran Sea)

Mapping Community Engagement with Urban Crowd-Sourcing

Cities are highly dynamic entities, with urban elements such as businesses, cultural and social Points-of- Interests (POIs), housing, transportation and the like, continuously changing. In order to maintain accurate spatial information in these settings, crowd-sourcing models of data collection, such as in OpenStreetMap (OSM), have come under investigation. Like many crowd-sourcing platforms (e.g., Wikipedia), these geo- wikis exhibit tailing-off activity, bringing into ques- tion their long-term viability. In this paper, we begin an investigation into the sustainability of urban crowd-sourcing, by studying the network structure and ge- ographical mapping of implicit communities of con- tributors in OSM. We observe that spatially clustered crowd-sourcing communities produce higher coverage than those with looser geographic affinity. We discuss the positive implications that this has on the future of urban crowd-sourcing.

Coeval Evolution of the Eastern Philippine Sea Plate and the South China Sea in the Early Miocene: Paleomagnetic and Provenance Constraints From ODP Site 1177

AbstractThe Philippine Sea Plate (PSP) and the South China Sea (SCS), located at the intersection of the Eurasian, Pacific, and Indo‐Australian plates, are key areas for global plate reconstruction. However, the relationships between the PSP and the SCS are still elusive. Here we report a new paleolatitude determination (16.0° ± 4.5°N at ∼20 Ma) from the Ocean Drilling Program Site 1177 in the Shikoku Basin (SB) within the eastern PSP. The new results indicate that the SB had a geographical affinity with the SCS in the early Miocene. Furthermore, the similarity in the U‐Pb zircon age spectra of early Miocene turbidites from SB Site 1177 and SCS Site X28 implies that the two sites might receive similar materials in the early Miocene. Based on the new paleolatitude, provenance results, and other constraints, we suggest that the eastern PSP might be coupled dynamically to the SCS in the early Miocene.

Comparative phylogeography uncovers evolutionary past of Holarctic dragonflies

Here, we investigate the evolutionary history of five northern dragonfly species to evaluate what role the last glaciation period may have played in their current distributions. We look at the population structure and estimate divergence times for populations of the following species: Aeshna juncea (Linnaeus), Aeshna subarctica Walker, Sympetrum danae (Sulzer), Libellula quadrimaculata Linnaeus and Somatochlora sahlbergi Trybom across their Holarctic range. Our results suggest a common phylogeographic pattern across all species except for S. sahlbergi. First, we find that North American and European populations are genetically distinct and have perhaps been separated for more than 400,000 years. Second, our data suggests that, based on genetics, populations from the Greater Beringian region (Beringia, Japan and China) have haplotypes that cluster with North America or Europe depending on the species rather than having a shared geographic affinity. This is perhaps a result of fluctuating sea levels and ice sheet coverage during the Quaternary period that influenced dispersal routes and refugia. Indeed, glacial Beringia may have been as much a transit zone as a refugia for dragonflies. Somatochlora sahlbergi shows no genetic variation across its range and therefore does not share the geographic patterns found in the other circumboreal dragonflies studied here. Lastly, we discuss the taxonomic status of Sympetrum danae, which our results indicate is a species complex comprising two species, one found in Eurasia through Beringia, and the other in North America east and south of Beringia. Through this study we present a shared history among different species from different families of dragonflies, which are influenced by the climatic fluctuations of the past.

Tibetan Y-STR trait in the eleven regions of the Qinghai-Tibet Plateau.

Y-Chromosomal short-tandem repeats (Y-STRs) could provide highly valuable information for forensic investigation and demographic studies. However, there is still no systematic Y-STR information on Tibetan as obtained from different regions of the broad Qinghai-Tibet Plateau. In this study, an analysis was conducted on 585 male individuals, classed into 3 different dialect branches as Ü-Tsang, Amdo, and Khams and originating from 11 scattered regions of the Qinghai-Tibet Plateau. The gene diversity values of the 41 Y-STRs in Tibetan ranged from 0.3636 to 0.9322. Additionally, a total of 563 distinct haplotypes were obtained with an overall haplotype diversity of 0.9999 and a discrimination capacity of 0.9624. As suggested by the inter-population diversity analysis, there were two main separated clades of Tibetan subgroups. The visualization of pairwise genetic distances between 11 Tibetan subgroups and 59 reference populations using cladogram revealed the distribution of various populations, which was basically consistent with the patterns of geographic origin and linguistic affinity.