Genetically Modified Varieties Research Articles

Effects of graphical presentation of benefits on cognitive judgments induced by affect heuristic: Focusing on the acceptance of genetically modified foods

Genetically modified (GM) foods have been assumed to be seen through intuitive and affective routes (i.e., affect heuristics) rather than analytical and deliberative routes. We examined the impact of the graphical presentation of benefits derived from GM or conventionally bred foods on the acceptance of these varieties. In the two experiments (n = 266 for study 1 and n = 402 for study 2), no differences emerged in the estimation of farmers' benefits resulting from the introduction of improved varieties by the type of improvement. However, there were statistically significant differences in the magnitude of risk and the degree of acceptance of the improved varieties. Therefore, despite presenting identical benefits as a graphical figure, GM foods were consistently evaluated as providing higher risk and were less frequently accepted than conventionally bred foods. These results suggest that while the graphical presentation of benefits may promote comprehension of some advantages of the introduction of GM varieties, this may not lead to acceptance from the consumer's point of view. Based on the current findings, as well as previous studies on trust in risk managers, we discuss the specific factors that might promote acceptance of GM products.

A simplified strategy based on the house of quality to prioritize farming practices under variable weather conditions

This work describes for the first time a strategy based on the house of quality analysis (HOQ) to select a farming practice adaptable to variable weather conditions (VWC). Five basic requirements: water, soil, fertilizers, insecticides, and herbicides, together with six agricultural practices: organic farming (OF), agroecology (AE), conservation agriculture (CA), genetically-modified varieties (GMV), intensified agriculture (IA), and climate smart agriculture (CSA) were correlated, assessed, and prioritized. HOQ concluded that water and soil are essential for the development of a crop, whereas the need of agrochemicals was less noticeable because they could be substituted by the use of sustainable practices. OF and AE resulted more convenient because they use sustainable methods to manage a crop. The efficacy of CA and GMV to face VWC was limited by factors like the weather conditions of the site, the type of crop, and costs. IA and CSA were satisfactory when financing for the development is available. The synergy observed among OF, AE, and CA would ease transition and/or their simultaneous adoption; it would improve sustainability, productivity, and profitability for the farmer as long as the needs of the region, management of resources, and interested parties are taken into account.

Effects of genotype and environment on the nutrient and metabolic profiles of soybeans genetically modified with epidermal growth factor or thioredoxin compared with conventional soybeans

Genetically modified (GM) soybean events CT-4025 and CT-1001, which contain genes encoding thioredoxin and epidermal growth factor, respectively, have previously been developed for use in cosmetic materials. In this study, compositional analyses and metabolic profiling were carried out to assess the unintended effects on the composition of metabolites between GM soybeans and non-GM soybeans. The soybeans were cultivated in 2016 and 2017 in two regions (Ochang and Jeonju) in the Republic of Korea. Statistical analyses were performed using univariate and multivariate methods. Results from the compositional analysis showed that the compositional variation in the tested GM varieties was within the range of conventional varieties. Furthermore, the results of multivariate analysis revealed that the growing year had a much stronger effect on soybean composition than did the growing site or genotypes. In the case of soybeans harvested in 2016, the levels of most amino acids decreased while those of secondary metabolites and sucrose increased, indicating that water stress due to low rainfall in 2016 induced metabolic changes. These results suggest that environmental factors have a major impact on the composition of GM and non-GM soybeans. We further demonstrated that metabolic approaches are useful for investigating the environmental change-induced metabolic network in GM crops intended for use in cosmetic materials.

Why Africa’s New Green Revolution is failing – Maize as a commodity and anti-commodity in South Africa

The South African Government has for the past two decades spent significant resources on introducing smallholders to Genetically Modified (GM) maize with the aim to make agriculture a way out of poverty. However smallholder farming continues to decline and poverty is on the rise in the country. The present paper aims to explain this failure of the government to support its smallholders by describing the intra-actions between maize, politics and technological development in South African history. Importantly maize is understood here as an agent in that its materialities are not only being impacted by, but are also having impact on the outcome of farming practices and wider political economies. The paper describes how maize, as a result of intra-action between maize biology and choices made by farmers, politicians and breeders during the colonial era and apartheid, developed in parallel as a commodity serving the settler farmers, and an anti-commodity, or escape crop, providing subsistence to marginalised smallholders. While South Africa today is a democracy that spends significant resources on improving smallholder livelihoods, recent technological development and market concentration have increased rather than decreased the gap between commodity- and anti-commodity maize. As a result new GM and hybrid maize varieties introduced to smallholders today are badly equipped to facilitate a crop led New Green Revolution.

GENETICALLY MODIFIED CORN ADOPTION IN BRAZIL, COSTS AND PRODUCTION STRATEGY

Genetically modified (GM) corn accounting for 90% of total area in Brazil. In this paper, we report the results of a four-year field survey (2010/11, 2013/14, 2014/15 and 2015/16) on corn production costs in Brazil, for two harvests and eleven states. The survey used both a quantitative and a qualitative approach: the first one compares GM and NonGM varieties costs in the two harvests, as well as the evolution of costs for GM corn, and the second part reports the results of a qualitative field survey. Results showed that GM corn costs are higher when compared to NonGM corn, due to higher GM seed prices, which more than compensate for the decrease in insecticides costs. When comparing the evolution of GM corn costs, seed costs tended to decrease, but insecticides had an increasing trend. The qualitative survey interviewed 314 agents, and the results showed that, in most cases, risk reduction, genetic potential, and low availability of NonGM seeds explained GM adoption. These two last factors may reflect a commercial strategy of seed companies, an issue that deserves more attention.

Monitoring of Genetically Modified Rice among Some Imported Rice Samples in Iran

Background: Genetically Modified (GM) foods are produced using genetic engineering. This survey attempted to identify the presence of GM rice varieties among some imported rice samples in Iran. Methods: From May to July 2016, a total of 50 samples of the imported rice to Iran were obtained, including 20 bulk rice samples from Bandar Abbas custom, Southern Iran and 30 retail rice samples from some commercial brands sold in the local markets of Yazd, Iran. Polymerase Chain Reaction was used to assess the GM varieties. Results: None of the studied rice samples had the target genes related to GM products. Conclusion: This study indicated no evidence for presence of GM rice among some bulk and retail imported rice samples in Iran. Since marketing of GM rice is not legally permitted in Iran, more comprehensive studies must be designed with higher sample size in various provinces of country to achieve more detailed data about situation of GM rice in the Iranian markets.

The impact of using genetically modified (GM) corn/maize in Vietnam: Results of the first farm-level survey

ABSTRACT This study assessed the farm-level economic and environmental impacts from the use of genetically modified (GM) corn in Vietnam (resistant to Lepidopteran pests of corn and tolerant to the herbicide glyphosate). It was largely based on a farmer survey conducted in 2018–19. The GM varieties out-performed conventional varieties in terms of yield by +30.4% (+15.2% if the yield comparison is with only the nearest performing equivalent conventional varieties) and reduced the cost of production by between US $26.47 per ha and US $31.30 per ha. For every extra US $1 spent on GM seed relative to conventional seed, farmers gained between an additional US $6.84 and US $12.55 in extra income. The GM maize technology also reduced insecticide and herbicide use. The average amount of herbicide active ingredient applied to the GM crop area was 26% lower (1.66 kg per ha) than the average value for the conventional corn area (2.26 kg/ai per ha) and in terms of the associated environmental impact of the herbicide use, as measured by the Environmental Impact Quotient (EIQ) indicator, it was lower by 36% than the average value applicable to the conventional corn area. Insecticides were used on a significantly lower GM crop area and, when used, in smaller amounts. The average amount of insecticide applied to the GM corn crop was significantly lower by 78% (0.08 kg/ai per ha) than the average value for the conventional corn area (0.36 kg/ai per ha) and in terms of the associated environmental impact of the insecticide use, as measured by the EIQ indicator, it was also lower by 77% than the average value for conventional corn (14.06 per ha).

Stacked genetically modified soybean harboring herbicide resistance and insecticide rCry1Ac shows strong defense and redox homeostasis disturbance after glyphosate-based herbicide application

BackgroundWorld agricultural production of genetically modified (GM) products, in particular, the combination of different traits/genes in the same plant has been a trend over the last decade. There have been concerns raised over stacking multiple herbicide and insect-resistant transgenes that could result in fitness costs depending on the type and strength of selection pressures exerted by the environment. Here, we report the results of transcriptomic analysis comparing the effect of glyphosate-based herbicide (GBH) in the single-transgene versus stacked, herbicide-resistant soybean varieties on various biological processes, metabolic pathways, and key shikimic enzymes.ResultsGene expression data showed that defense metabolism and redox homeostasis were equally modulated in single-transgene and stacked-variety samples. Carbon accumulation and energy metabolisms were distinct between the varieties and photosynthesis metabolism was found negatively affected in the single-transgene variety only. In the stacked variety, the shikimate pathway was modulated by the accumulation of transcripts from phenylalanine gene and other cascade genes. As expected, the expression of native EPSPS was upregulated in both varieties when herbicide was applied. On the other hand, transgenic EPSPS expression was down-regulated in both GM varieties upon herbicide application which cannot be explained.ConclusionGlyphosate-based herbicides toxicity suggests its effects on carbon central metabolism and flux, redox metabolism, photosynthesis, and to hormone and defense response in plants. The observed unintended effects in GM herbicide-tolerant varieties unravel the deleterious effects previously observed on GM-tolerant varieties growth and production. The impact of GBH on shikimate and cascade pathways was observed in terms of both native and transgenic insensitive EPSPS modulation, alteration of jasmonic acid and lignin metabolism in both single-transgene and stacked variety. The energy metabolism and carbon flux were differently affected in these varieties. Oxidative stress, more specifically glutathione metabolism, induced by GBH, was also observed in this study. The stacked variety showed a more pronounced stress response (activation of specific stress defense proteins, Rboh, WRKY) and secondary compounds (β-glucosidase, isoflavone 7-O-methyltransferase). Omics profiling techniques, such as transcriptomics, can be considered tools to support risk assessment in detecting unintended effects due to the GBH application.

Evaluation of the effects of sugarcane processing on the presence of GM DNA and protein in sugar

ABSTRACT The Brazilian Sucro-energy Sector produces both energy, in the form of ethanol fuel, industrial steam and electricity, and sugar. Centro de Tecnologia Canavieira (CTC), the leading Brazilian sugarcane breeding company, has developed a pipeline of insect-protected sugarcane varieties to control sugarcane borer damage. The goal of this manuscript is to present the results of studies with three genetically modified (GM) sugarcane varieties and to evaluate the published literature regarding the possible presence of GM sugarcane DNA or protein in raw or refined sugar. Specifically, two varieties of approved GM sugarcane, CTC91087-6 and CTC175-A, and an experimental CTC variety, were grown in four individual plots to produce four batches each of processed raw sugar using standard smaller-scale laboratory processing methods resulting in a total of 12 independent batches of raw sugar. Herein, we report the development of event-specific probes and DNA detection methods, designed to detect the junction of sugarcane genomic DNA and the inserted DNA of the two approved GM varieties. An identical approach was used for the testing of sugar made from the experimental CTC variety. The methodology used TaqMan® real-time PCR and ELISA assays validated for the four GM proteins expressed by these three events (Cry1Ab, Cry1Ac, NPTII, and PAT (bar)). The developed assays had very low limits of detection (LODs) for the various event-specific DNA probes (7.2–25 ng/g sugar) and insecticidal and selectable marker proteins (2.9–10.9 ng/g sugar). No event-specific DNA and no GM proteins were detectable in the 12 independent batches of raw sugar produced from these three GM sugarcane events. The results of this study, using very sensitive methods and testing several sugar batches, extend the conclusions of previous studies, reviewed herein, that showed the extensive degradation and removal of DNA and protein during sugarcane processing. Overall, these results indicate that there are no distinguishable differences between the highly purified, chemically defined sugar produced from conventional or GM varieties.

Using a zero-inflated model to assess gene flow risk and coexistence of Brassica napus L. and Brassica rapa L. on a field scale in Taiwan

BackgroundThe cropping area of genetically modified (GM) crops has constantly increased since 1996. However, currently, cultivating GM crops is associated with many concerns. Transgenes are transferred to non-GM crops through pollen-mediated gene flow, which causes environmental problems such as superweeds and introgressive hybridization. Rapeseed (Brassica napus L.), which has many GM varieties, is one of the most crucial oil crops in the world. Hybridization between Brassica species occurs spontaneously. B. rapa grows in fields as a weed and is cultivated as a crop for various purposes. Both B. rapa weeds and crops participate in gene flow among rapeseed. Therefore, gene flow risk and the coexistence of these two species should be studied.ResultsIn this study, field experiments were conducted at two sites for 4 years to evaluate gene flow risk. In addition, zero-inflated models were used to address the problem of excess zero values and data overdispersion. The difference in the number of cross-pollination (CP) events was nonsignificant between upwind and downwind plots. The CP rate decreased as the distance increased. The average CP rates at distances of 0.35 and 12.95 m were 2.78% and 0.028%, respectively. In our results, zero-inflated negative binomial models were comprehensively superior to zero-inflated Poisson models. The models predicted isolation distances of approximately 1.36 and 0.43 m for the 0.9% and 3% threshold labeling levels, respectively.ConclusionsCultivating GM crops is prohibited in Taiwan; however, the study results can provide a reference for the assessment of gene flow risk and the coexistence of these two species in Asian countries establishing policies for GM crops.

A universal analytical approach for screening and monitoring of authorized and unauthorized GMOs

Release of various genetically modified organisms (GMOs) into market during the past decade contributes to concerns regarding the traceability of food and feed products. In this study, a comprehensive list of inserted elements of commercialized GMOs has been collected, which contained information of 191 singular genetically modified (GM) varieties. Several elements were selected to develop a universal analytical approach for screening GM presence in food and feed products. This approach achieved coverage of those singular GM events as well as their hybrid crosses. The developed screening approach showed great specificity and sensitivity of less than 25 copies that meet the labeling demands for all countries. Moreover, microfluidic chip and digital PCR were combined in this approach to detect GM varieties and estimate GM content, both of which achieved high-throughput and accurate identification of unauthorized GMOs. Overall, this new analytical approach will serve as a functional tool for accurate control of authorized and unauthorized GMOs.

Determinants of Food Security Status amongst Smallholder Farmers Utilizing Different Maize Varieties in OR Tambo District, South Africa

Maize is an important staple crop for poverty reduction and global food security in Sub Saharan Africa. Food insecurity can be combated through adoption of productivity improving technologies, which include improved maize varieties. In that endeavour, South Africa has promoted various improved maize varieties which include open pollinated varieties (OPVs), hybrids, and genetically modified (GM) varieties. Despite this, the traditional landrace varieties have also been dominating in the country. However, the household food insecurity problem in the Eastern Cape Province of South Africa may signify a mis-match between maize varieties being promoted amongst smallholder farmers’ and their needs. It therefore necessitates a scrutiny of the food security status among users of different maize varieties, and the determinants of such food security. A cross sectional survey was conducted in Port St Johns, Mqanduli and Flagstaff in the Eastern Cape Province, South Africa. Data was purposively collected from a sample of 650 smallholder farmers using a structured questionnaire. Descriptive statistics, Household Food Insecurity Access Score and ordinal logistic regression model were employed to characterize, examine the household food insecurity status and the determinants, respectively. Fifty-six percent of the respondents were utilizing land race maize varieties, whilst 29% GMs, 10% combining GMs and landrace, 4% improved OPVs and 1% convectional hybrids. The average land area under maize was 1.09 hectares with average yields (t/ha) of 1.9, 0.5, 1.7 and 1.6 for GM, landrace, conventional hybrids and improved OPVs respectively. Fifty-five percent of households utilizing GM varieties and 61% of those combining maize varieties were food secure. The regression model showed that maize variety had significant influence on food security. The study found that GM maize, improved OPV, white maize and combination effects of GM maize was associated with reduction of household food insecurity. From the study, it can be put into perspective that use of white and improved maize varieties reduces household food insecurity. Therefore, to address household food insecurity, the study recommended targeting white maize varieties, especially GM white maize varieties which are highly productive and a positive influence on household food security.

High-throughput sequencing analysis of microbial community diversity in response to indica and japonica bar-transgenic rice paddy soils.

Potential environmental risks of genetically modified (GM) crops have raised concerns. To better understand the effect of transgenic rice on the bacterial community in paddy soil, a field experiment was carried out using pairs of rice varieties from two subspecies (indica and japonica) containing bar transgene with herbicide resistance and their parental conventional rice. The 16S rRNA gene of soil genomic DNA from different soil layers at the maturity stage was sequenced using high-throughput sequencing on the Illumina MiSeq platform to explore the microbial community diversity among different rice soils. There were no significant differences in diversity indices between transgenic japonica rice and its sister conventional rice (japonica pair) among different soil layers, but, significant differences was observed between transgenic indica rice and its conventional rice (indica pair) in the topsoil layer around concentrated rice roots according to the ace diversity index. Though the japonica rice soil and indica rice soil were shared several key genera, including Rivibacter, Anaeromyxobacter, Roseomonas, Geobacter, Thiobacillus, Clostridium, and Desulfobulbus, the primary bacterial genera in indica rice soil were different from those in japonica rice. Synechococcus and Dechloromonas were present in japonica rice samples, while Chloronema, Flexibacter, and Blastocatella were observed in indica rice soil. Moreover, the abundance of genera between GM and non-GM varieties in japonica rice was significantly different from indica rice, and several bacterial communities influenced these differences. Anaerovorax was more abundant in transgenic japonica rice soil than conventional rice soil, while it was deficient in transgenic indica rice soil compared to conventional rice soil, and opposite responses to Deferrisoma were in that of indica rice. Thus, we concluded that transgenic indica and japonica rice had different effects on soil bacteria compared with their corresponding sister conventional rice. However, these composition and abundance difference only occurred for a few genera but had no effect on the primary genera and soil characteristics were mainly contributed to these differences. Thus, differences in bacterial community structure can be ignored when evaluating the impacts of transgenic rice in the complex soil microenvironment.

Use of omics analytical methods in the study of genetically modified maize varieties tested in 90 days feeding trials

Genetically modified (GM) maize and their non-modified counterparts were compared using MON810 varieties, the only GMO event cultivated in Europe. The differences in grain samples were analysed by omics profiles, including transcriptomics, proteomics and metabolomics. Other cultivated maize varieties were analysed as a reference for the variability that will exist between cultivated varieties. The observed differences between modified and non-modified maize varieties do not exceed typical differences between non-modified varieties. The use of these advanced analytical approaches to analyse novel plant materials as compared to the results from animal feeding trials with whole foods is assessed. No indications were observed for changes in the GM varieties that warrant further investigations. Furthermore, it was shown that such indications will be obtained if maize samples of inferior quality are analysed similarly. Omics data provide detailed analytical information of the plant material, which facilitates a risk assessment procedure of new (GM) plant varieties.

Unintended effects of a Phytophtora-resistant cisgenic potato clone on the potato aphid Macrosiphum euphorbiae and its parasitoid Aphidius ervi

Genetically modified (GM) plants may show unintended differences compared to the original varieties, due to the modification process. Such differences might in some cases affect non-target organisms linked to the crop into an agro-ecosystem. In this paper, we aimed to study interactions of two blight-resistant GM potato clones with the aphid species Macrosiphum euphorbiae Thomas, a non-target arthropod frequently feeding on potato plants and one of the major pests of that crop. One of the potato events used in our experiments caused an increased fertility of the aphids in the first generation, and consequently, a positive effect on the growth of the aphid population was estimated. When a second generation of the aphid was reared on potato leaves of the same GM event, differences in aphid fertility were no longer observed. Behavioural studies conducted in a wind tunnel using the aphid parasitoid Aphidius ervi Haliday indicated that neither of the two tested GM varieties had a significant effect on the attractiveness of potato plants towards the parasitoid. In planta tests proved to be sensitive protocols to detect unintended effects on a non-target arthropod; experimental results, however, indicate that these effects are not expected to be biologically relevant in this tritrophic system, if these GM events become available for commercial use in the future.

An overview of genetically modified crop governance, issues and challenges in Malaysia.

The application of agricultural biotechnology attracts the interest of many stakeholders. Genetically modified (GM) crops, for example, have been rapidly increasing in production for the last 20 years. Despite their known benefits, GM crops also pose many concerns not only to human and animal health but also to the environment. Malaysia, in general, allows the use of GM technology applications but it has to come with precautionary and safety measures consistent with the international obligations and domestic legal frameworks. This paper provides an overview of GM crop technology from international and national context and explores the governance and issues surrounding this technology application in Malaysia. Basically, GM research activities in Malaysia are still at an early stage of research and development and most of the GM crops approved for release are limited for food, feed and processing purposes. Even though Malaysia has not planted any GM crops commercially, actions toward such a direction seem promising. Several issues concerning GM crops as discussed in this paper will become more complex as the number of GM crops and varieties commercialised globally increase and Malaysia starts to plant GM crops. © 2017 Society of Chemical Industry.

Are GM Crops for Yield and Resilience Possible?

Crop yield improvements need to accelerate to avoid future food insecurity. Outside Europe, genetically modified (GM) crops for herbicide- and insect-resistance have been transformative in agriculture; other traits have also come to market. However, GM of yield potential and stress resilience has yet to impact on food security. Genes have been identified for yield such as grain number, size, leaf growth, resource allocation, and signaling for drought tolerance, but there is only one commercialized drought-tolerant GM variety. For GM and genome editing to impact on yield and resilience there is a need to understand yield-determining processes in a cell and developmental context combined with evaluation in the grower environment. We highlight a sugar signaling mechanism as a paradigm for this approach.

China agrees to review U.S. biotech applications

China will evaluate eight pending U.S. agricultural biotechnology product applications by the end of May, potentially opening the door for sales by Dow AgroSciences, DuPont Pioneer, Monsanto, and Syngenta. China agreed to conduct the evaluations as part of an agreement unveiled by the White House on May 12. The two countries reached the trade deal after a meeting in April between U.S. President Donald J. Trump and Chinese President Xi Jinping. U.S. officials have prodded China for years to speed up its lengthy process for deciding whether to approve the import of new genetically modified (GM) crops. It typically takes six years to win Chinese clearance of a GM variety, twice as long as other major nations take. Under the new deal, China’s National Biosafety Committee will meet by the end of May to assess the safety of eight products made by four major U.S. agrochemical companies. Dow AgroSciences is

Pollen-mediated gene flow and seed exchange in small-scale Zambian maize farming, implications for biosafety assessment.

Gene flow in agricultural crops is important for risk assessment of genetically modified (GM) crops, particularly in countries with a large informal agricultural sector of subsistence cultivation. We present a pollen flow model for maize (Zea mays), a major staple crop in Africa. We use spatial properties of fields (size, position) in three small-scale maize farming communities in Zambia and estimate rates of cross-fertilisation between fields sown with different maize varieties (e.g. conventional and transgene). As an additional factor contributing to gene flow, we present data on seed saving and sharing among farmers that live in the same communities. Our results show that: i) maize fields were small and located in immediate vicinity of neighboring fields; ii) a majority of farmers saved and shared seed; iii) modeled rates of pollen-mediated gene flow showed extensive mixing of germplasm between fields and farms and iv) as a result, segregation of GM and non-GM varieties is not likely to be an option in these systems. We conclude that the overall genetic composition of maize, in this and similar agricultural contexts, will be strongly influenced both by self-organised ecological factors (pollen flow), and by socially mediated intervention (seed recycling and sharing).

Economic Valuation of GM Technology:A Literature Review

Genetically modified (GM) technology has been part of the agricultural landscape for more than 20 years and has now been adopted on more than 181 million hectares (ha) in both developed countries (47%) and developing countries (53%). This paper is a review of existing literature on economic valuation of GM technology, focusing on the first generation GM varieties. Valuations at the micro and aggregate level are discussed with respect to specific parameter values, and other aspects such as environmental and regulation concerns were identified that can be included in further research so as to ensure more comprehensive results of the economic valuation of the GM technology.