Genetic Modification Of Bacteria Research Articles

Acceleration of Bio-Ethanol Production from Waste Vegetables and Fruits by Using Zymomonas mobilis Genetically Modified

The genetic modification pathways have been considered the shortest and most efficient for the production of bioethanol, in particular, that knock-in Alcohol dehydrogenase (ADH1) originally from Saccharomyces cerevisiae into the overexpression plasmid (pET28a), in order to continuous stimulate and accelerate the fermentation after inserted directly into the Zymomonas mobilis bacteria. At the same time, there are huge volumes of waste vegetables and fruit which are produced every day from restaurants and shops, and need to eliminate them with modern scientific methods, in order to the clean the environment and produced biofuel to reduce at least the use of fossil fuels. The percentage of production bioethanol was 52 % / 172 hours, which is considered a qualitative leap in the percentage of production during that period. In contrast, HPLC technique has determined the purity of bioethanol was 89.07% compared to 99% absolute ethanol which is possible to increase the purity according to the biochemical method. This study illustrated that may be commercially bioethanol produced in the future by the development of technology-based on genetic modification of bacteria.

Genetic engineering of bacteria from managed and natural habitats.

The genetic modification of bacteria from natural and managed habitats will impact on the management of agricultural and environmental settings. Potential applications include crop production and protection, degradation or sequestration of environmental pollutants, extraction of metals from ores, industrial fermentations, and productions of enzymes, diagnostics, and chemicals. Applications of this technology will ultimately include the release of beneficial agents in the environment. If safely deployed, genetically modified bacteria should be able to provide significant benefits in the management of environmental systems and in the development of new environmental control processes.