Genetic Engineering Techniques Research Articles

Genomic Advances in Biofortification of Iron and Zinc in Wheat (Triticum aestivum L.)

Majority of population that depend on wheat are found to be deficient in iron and zinc. About 20% of calories worldwide are derived from wheat. Milling of wheat reduces the bioavaibility of Fe and Zn in wheat grains. Micronutrient deficiency is the main cause of malnutrition worldwide and hidden hunger in underdeveloped countries. To overcome this problem it becomes necessary to develop varieties that are rich in iron and zinc content. Biofortification gets around these issues by enhancing the micronutrient content of wheat. The genetic approaches of biofortification particularly the non-conventional methods have proved to be the most efficient ones. These method includes QTL mapping approach, GWAS and transgenics. Although conventional methods for a long time has been used for developing varieties of wheat rich in Fe and Zn but conventional breeding for certain traits may be inconvenient and time-consuming and are prone to errors due to their reliance on traditional phenotyping or visual assessments. Several research have been undertaken to map QTLs of Fe and Zn concentration in wheat grains and have been used in elite genetic backgrounds to improve micronutrient content. Genomic studies revealed through GWAS exhibit high QTL resolution and allele coverage, which can be used for improvement of Fe and Zn content in wheat. Genetic engineering techniques or transgenics approaches allows genetic alteration of crops to improve the nutritional quality of wheat. Genomic approach of biofortification of wheat has proved to be the most efficient methods for increasing iron and zinc content in the crop.

Membraneless organelles assembled by AuNPs-enzyme integration in non-photosynthetic bacteria: Achieving high specificity and selectivity for solar hydrogen production

The emergence of semiartificial photosynthesis holds great promise for improving light energy capture and conversion efficiency. However, achieving high product specificity and selectivity in semiartificial photosynthetic systems remains a challenge. In this study, we employed genetic engineering techniques to construct a membraneless organelle in E. coli, serving as the photoreaction carrier. The self-assembly of photosensitizers and hydrogenase within the carrier has successfully established a semiartificial photosynthetic model characterized by high product specificity and selectivity. In this model system, electrons were directly transferred to hydrogenase upon AuNPs light excitation in membraneless organelle, eliminating the need for additional electron mediators and bypassing the use of intracellular NADH/NAD+ as a reducing agent to avoid byproduct formation. The semiartificial photosynthetic system demonstrated an 11782-fold and 40-fold increase in hydrogen production compared to bare AuNPs and the empty vector E. coli hybrid system, respectively. This membraneless organelle holds the potential to robustly support the specific synthesis of high-energy-density, value-added compounds through solar energy conversion.

Floriculture and Transgenic

“Genetic engineering has revolutionized floriculture by enabling the modification of plant species and the creation of new flower color varieties (Chandler, Stephen et al, 2010). Despite the global cultivation of transgenic crops, floriculture has been slow to adopt transgenic breeding. "Floriculture, with its cut flower market as a significant component, is on track to achieve a market size of $43.8 billion by 2027, according to Markets and Markets. This industry plays a crucial role in uplifting rural economies through international trade. For instance, floriculture products from Africa find their way to markets in the U.S. and Europe. Similarly, Asian nations such as Japan, China, and India are strengthening their financial positions through the import and export of floriculture commodities (Tanaka, Yoshizu et al., 2009). One of the notable advancements in floriculture has been the creation of new flower color varieties by manipulating pigment metabolic pathways in plants. However, there are challenges. Some flowering plants, despite their high market value, do not realize their full economic potential due to issues like prolonged flowering seasons and diseases." These challenges can be addressed through the production of transgenic plants using genetic engineering and plant tissue culture techniques. The study aims to explore the potential of genetic engineering associated with plant tissue culture techniques in enhancing floriculture, particularly in developing new flower color varieties and improving the production of high-value flowering plants, thereby highlighting the economic impact of floriculture globally.

Engineering Microorganisms for Cancer Immunotherapy.

Cancer immunotherapy presents a promising approach to fight against cancer by utilizing the immune system. Recently, engineered microorganisms have emerged as a potential strategy in cancer immunotherapy. These microorganisms, including bacteria and viruses, can be designed and modified using synthetic biology and genetic engineering techniques to target cancer cells and modulate the immune system. This review delves into various microorganism-based therapies for cancer immunotherapy, encompassing strategies for enhancing efficacy while ensuring safety and ethical considerations. The development of these therapies holds immense potential in offering innovative personalized treatments for cancer.

The commercial potential of Aphanizomenon flos-aquae, a nitrogen-fixing edible cyanobacterium

AbstractAphanizomenon flos-aquae (AFA) is a Gram-negative nitrogen-fixing freshwater filamentous cyanobacterium with a rich nutritional profile that is approved for human consumption by both the Food and Drug Administration and the European Food and Safety Authorities. It has a high protein content (60-70%) and contains numerous vitamins, minerals and trace elements together with several high-value compounds with nutraceutical properties, such as C-phycocyanin and β-phenylethylamine. 500-1000 t of AFA dry biomass are currently wild harvested from natural blooms that occur seasonally in Klamath Lake, Oregon, USA, and distributed as a nutritional supplement worldwide. The requirements and unreliability of wild harvesting, owing to the dependence of AFA growth on environmental conditions and potential contamination by microcystin toxins, threaten the availability of biomass supply and restricts commercial expansion. In this review we propose AFA cultivation in open ponds or closed photobioreactors to obtain a reliable production of unialgal biomass to resolve the supply issue and enhance AFA as a feedstock for specific high-value by-products. Furthermore, we explore potential strategies for maximising overall yield and seasonal robustness by using a synergistic combination of cultivation and wild harvesting. Controlled AFA cultivation would also facilitate the use of genetic manipulation to generate bespoke strains with improved commercial applications, such as increasing the cyanobacterium’s nitrogen-fixation rate to enhance its value as a biofertiliser. Ultimately, realising the untapped biotechnological potential of AFA requires a better understanding of its fundamental biology, robust methodologies for laboratory and large-scale cultivation, and the development of AFA-specific genetic engineering techniques.

What is the role of microbial biotechnology and genetic engineering in medicine?

Microbial products are essential for developing various therapeutic agents, including antibiotics, anticancer drugs, vaccines, and therapeutic enzymes. Genetic engineering techniques, functional genomics, and synthetic biology unlock previously uncharacterized natural products. This review highlights major advances in microbial biotechnology, focusing on gene-based technologies for medical applications.

Horticulture crop under pressure: Unraveling the impact of climate change on nutrition and fruit cracking

Climate change is increasingly affecting the nutritional content and structural integrity of horticultural crops, leading to challenges such as diminished fruit quality and the exacerbation of fruit cracking. This manuscript systematically explores the multifaceted impacts of these changes, with a particular focus on the nutritional quality and increased incidence of fruit cracking. An exhaustive review of current research identifies the critical role of transcription factors in mediating plant responses to climatic stressors, such as drought, temperature extremes, and saline conditions. The significance of transcription factors, including bHLH, bZIP, DOF, MDP, HD-ZIP, MYB, and ERF4, is highlighted in the development of fruit cracking, underscoring the genetic underpinnings behind stress-related phenotypic outcomes. The effectiveness of greenhouse structures in mitigating adverse climatic effects is evaluated, offering a strategic approach to sustain crop productivity amidst CO2 fluctuations and water scarcity, which are shown to influence plant physiology and lead to changes in fruit development, nutrient dynamics, and a heightened risk of cracking. Moreover, the manuscript delves into advanced breeding strategies and genetic engineering techniques, such as genome editing, to enhance crop resilience against climatic challenges. It also discusses adaptation strategies vital for sustainable horticulture, emphasizing the need to integrate novel genetic insights with controlled environment horticulture to counteract climate change's detrimental effects. The synthesis presented here underscores the urgent need for innovative breeding strategies aimed at developing resilient crop varieties that can withstand climatic uncertainty while preserving nutritional integrity.

Modern Approaches to Enhancing Rust Resistance in Wheat Leading to Global Food Security

Rust diseases pose significant threats to wheat production. The deployment of wheat cultivars endowed with rust resistance stands as the most potent strategy for effective rust management. This resistance is primarily inherited through Mendelian principles discovered in 1905, but traditional breeding methods are time-consuming. Modern strategies have emerged to develop rust-resistant wheat varieties efficiently. Marker-Assisted Selection (MAS) accelerates the breeding process through precise screening, bringing about a revolution in the creation of rust-resistant wheat varieties. Genetic engineering techniques allow the transfer of resistance genes from other species into susceptible crops, but GMO use remains controversial and regulated. Gene editing, especially with CRISPR-Cas9, is a game-changer, enabling the introduction of natural variations or inactivation of critical genes in rust pathogens, enhancing plant resistance. RNA interference (RNAi) is another promising strategy, using small RNA molecules to inhibit rust pathogen gene expression, reducing disease severity. Induced Systemic Resistance (ISR) primes plant immune systems by treating them with beneficial microorganisms or compounds, fortifying them against subsequent rust infections. Eco-friendly biofungicides with antagonistic microorganisms suppress rust infections as alternatives to chemical fungicides. The development of climate-resilient wheat varieties is essential, as they indirectly enhance rust resistance, ensuring stable production amid changing climate conditions. These efforts to improve wheat productivity and rust resistance are crucial for feeding the growing global population. Integrating modern methods with traditional breeding is key to effectively combatting rust diseases and enhancing food security.

Aspergillus oryzae as a Cell Factory: Research and Applications in Industrial Production.

Aspergillus oryzae, a biosafe strain widely utilized in bioproduction and fermentation technology, exhibits a robust hydrolytic enzyme secretion system. Therefore, it is frequently employed as a cell factory for industrial enzyme production. Moreover, A. oryzae has the ability to synthesize various secondary metabolites, such as kojic acid and L-malic acid. Nevertheless, the complex secretion system and protein expression regulation mechanism of A. oryzae pose challenges for expressing numerous heterologous products. By leveraging synthetic biology and novel genetic engineering techniques, A. oryzae has emerged as an ideal candidate for constructing cell factories. In this review, we provide an overview of the latest advancements in the application of A. oryzae-based cell factories in industrial production. These studies suggest that metabolic engineering and optimization of protein expression regulation are key elements in realizing the widespread industrial application of A. oryzae cell factories. It is anticipated that this review will pave the way for more effective approaches and research avenues in the future implementation of A. oryzae cell factories in industrial production.

Breeding for water-use efficiency in wheat: progress, challenges and prospects.

Drought poses a significant challenge to wheat production globally, leading to substantial yield losses and affecting various agronomic and physiological traits. The genetic route offers potential solutions to improve water-use efficiency (WUE) in wheat and mitigate the negative impacts of drought stress. Breeding for drought tolerance involves selecting desirable plants such as efficient water usage, deep root systems, delayed senescence, and late wilting point. Biomarkers, automated and high-throughput techniques, and QTL genes are crucial in enhancing breeding strategies and developing wheat varieties with improved resilience to water scarcity. Moreover, the role of root system architecture (RSA) in water-use efficiency is vital, as roots play a key role in nutrient and water uptake. Genetic engineering techniques offer promising avenues to introduce desirable RSA traits in wheat to enhance drought tolerance. These technologies enable targeted modifications in DNA sequences, facilitating the development of drought-tolerant wheat germplasm. The article highlighted the techniques that could play a role in mitigating drought stress in wheat.

Post-harvest Biotechnology or Genetic Engineering Solutions: Extending Shelf Life and Reducing Food Waste

Post-harvest losses and food waste have become critical challenges in the global food supply chain, contributing to economic losses, environmental degradation, and food insecurity. This article explores the innovative applications of post-harvest biotechnology and genetic engineering as promising solutions to address these issues by extending the shelf life of perishable products and minimizing food waste. Advancements in genetic engineering techniques have paved the way for the development of crops with enhanced resistance to pests, diseases, and environmental stresses. Additionally, the manipulation of genes associated with ripening and senescence has allowed scientists to engineer fruits and vegetables with extended shelf life. These genetically modified organisms (GMOs) exhibit improved post-harvest characteristics, providing a longer window for transportation, storage, and consumption. Biotechnological interventions also include the use of biocontrol agents and beneficial microorganisms to suppress post-harvest pathogens, thereby reducing spoilage and decay. The development of bio-preservatives, such as antimicrobial peptides and natural compounds, offers an eco-friendly alternative to traditional chemical preservatives, contributing to both food safety and sustainability. Furthermore, the integration of smart packaging technologies with genetic modifications enhances the monitoring and control of environmental conditions during storage and transportation. Intelligent packaging materials equipped with sensors can detect changes in temperature, humidity, and gas composition, enabling real-time adjustments to prolong the freshness of perishable goods.

Exploring macrophytes’ microbial populations dynamics to enhance bioremediation in constructed wetlands for industrial pollutants removal in sustainable wastewater treatment

AbstractToxic contaminants from intense industrial operations are entering wetlands, harming human health and biodiversity. Macrophytes serve as principal producers in aquatic environments including natural wetlands, providing shelter, food, and, most crucially, intricate relationships with the surrounding microbial assemblage for support and microorganisms attachment. Wetlands have been nature's kidneys, for filtering water. Recent research has examined macrophytes' phytoremediation abilities. With recent improvements focused on engineered wetland technology, microbiological characterization, and genetic engineering, phytoremediation strategies have also benefited. However, little research has examined the role surrounding microbial population play on macrophyte efficiency in pollutant degradation, the extent and even mechanisms of these interactions, and their potential utility in wastewater treatment of diverse industrial effluents. Our bid for greener solutions implies that macrophyte-microorganisms’ interspecific interactions for in situ treatment of effluents should be optimised to remove contaminants before discharge in natural waterbodies or for recycle water usage. This review provides for the varied types of plants and microbial interspecific interactions beneficial to effective phytoremediation processes in artificial wetland design as well as considerations and modifications in constructed wetland designs necessary to improve the bioremediation processes. Additionally, the review discusses the latest advancements in genetic engineering techniques that can enhance the effectiveness of phyto-assisted wastewater treatment. We will also explore the potential utilisation of invasive species for their demonstrated ability to remove pollutants in the controlled setting of constructed wetlands.

Heterologous expression of taxane genes confers resistance to fall armyworm in Nicotiana benthamiana.

Taxadiene synthase, taxadiene-5α-hydroxylase, and taxane 13α-hydroxylase genes were introduced into Nicotiana benthamiana, and the improved resistance to lepidoptera pest fall armyworm was reported. Fall armyworm (FAW) is a serious agricultural pest. Genetic engineering techniques have been used to create pest-resistant plant varieties for reducing pest damage. Paclitaxel is a diterpenoid natural metabolite with antineoplastic effects in medicine. However, the effects of taxanes on the growth and development of lepidoptera pests, such as the FAW, are unknown. Here, selected paclitaxel precursor biosynthesis pathway genes, taxadiene synthase, taxane 5α-hydroxylase, and taxane 13α-hydroxylase, were engineered in the heterologous host Nicotiana benthamiana plants. Bioassay experiments showed that the transgenic N. benthamiana plants displayed improved resistance to FAW infestation, with degeneration of gut tissues and induced expression of apoptosis-related genes. Cytotoxicity experiment showed that the paclitaxel precursor, 10-deacetylbaccatin III, is cytotoxic to Sf9 cells, causing cell cycle arrest at the G2/M phase and disorder of the cytoskeleton. Metabolome analysis showed that heterologous expression of taxane genes in N. benthamiana affected the digestive system, steroid hormone and purine metabolism pathways of FAW larvae. In summary, this study provides a candidate approach for FAW control.

Advances of Structural Design and Biomedical Applications of Tobacco Mosaic Virus Coat Protein

Recognized as the primary RNA virus to be categorized and extensively studied, the tobacco mosaic virus (TMV) is foundational to advancements in virology, molecular biology, and biomaterials. Over the past few decades, the deep comprehension of the TMV coat protein (TMVcp) molecular structure and assembly principles has stimulated a surge in research on TMVcp structural design using genetic engineering and chemical modification techniques. The unique characteristics of TMVcp, including its nanoscale orderly structure, ease of modification, and considerable drug loading capacity, have enabled significant progress in its biomedical applications. This review summarizes the advanced strategies deployed for TMVcp design and multidimensional assembly and underscores the prototypical applications of TMVcp‐based biomaterials in bioimaging, drug delivery, tissue engineering, and biosensing. Ultimately, the future prospects of TMVcp research in structural design and biomedical applications are explored, which encompass artificial intelligence‐guided structural and functional design, the development of stimulus‐responsive biomaterials, and potential clinical translation.

A Literary Review on Pumsavana Karma

Ayurveda is the science of life and it is one of the most ancient healing methodologies in the world. This system is transmitted from immortals to mortals. There are various Samhitas written by our Acharyas related to health of the human beings. Pumsavana karma is one of the most important Samskara mentioned in Ayurveda. It is one of the Shodasha Samskaras performed in various stages of life in ancient India explained by scholars of Ayurveda. It can be done before the conception or immediately after the conception to achieve healthy and desired progeny during Pushya nakshatra. In Ayurveda, Pushya nakshatra is often considered a favourable time for various health related activities. It is believed that beginning Ayurvedic treatments during Pushya nakshatra can enhance their effectiveness and yield better results. It deals with the technique of genetic engineering and it can be considered to have some action on human genetics to ensure healthy progeny. Moreover, the complexion, glow, mental health of child can be improved through Pumsavana karma. In this article the importance of Pumsavana karma has described that which is not only for desired sex but also to achieve healthy progeny by using different formulations mentioned in various Samhitas was explained.

Advanced Biotechnological Interventions in Mitigating Drought Stress in Plants.

This comprehensive article critically analyzes the advanced biotechnological strategies to mitigate plant drought stress. It encompasses an in-depth exploration of the latest developments in plant genomics, proteomics, and metabolomics, shedding light on the complex molecular mechanisms that plants employ to combat drought stress. The study also emphasizes the significant advancements in genetic engineering techniques, particularly CRISPR-Cas9 genome editing, which have revolutionized the creation of drought-resistant crop varieties. Furthermore, the article explores microbial biotechnology's pivotal role, such as plant growth-promoting rhizobacteria (PGPR) and mycorrhizae, in enhancing plant resilience against drought conditions. The integration of these cutting-edge biotechnological interventions with traditional breeding methods is presented as a holistic approach for fortifying crops against drought stress. This integration addresses immediate agricultural needs and contributes significantly to sustainable agriculture, ensuring food security in the face of escalating climate change challenges.

A Study on the Application of Recombinant Factor C (rFC) Assay Using Biopharmaceuticals.

Gram-negative bacterial endotoxins can cause pathophysiological effects such as high fever when introduced into the bloodstream. Therefore, endotoxin testing is necessary when producing injectable pharmaceuticals. The pharmaceutical industry has widely used Limulus amebocyte lysate (LAL) to certify product quality. However, ethical concerns have been raised and the increasing scarcity of Limulus polyphemus necessitates the development of novel testing techniques. Recombinant factor C (rFC) was developed using genetic engineering techniques. The aim of this study was to investigate the validity of rFC testing and compare it with the LAL method. The specificity, linearity, accuracy, precision, and robustness of the rFC assay were evaluated. After validation, the rFC assay was found to be suitable for endotoxin detection. We compared the accuracy of the rFC and LAL assays using reference standard endotoxin. The rFC assay was as accurate as the LAL assay. We also compared the two assays using biopharmaceuticals. Greater interference occurred in some samples when the rFC assay was used than when the LAL assay was used. However, the rFC assay overcame the interference when the samples were diluted. Overall, we suggest that rFC can be applied to test biopharmaceuticals.

From Lab to Bouquet: The Biotechnological Frontier in Modern Floriculture for Sustainable and Resilient Flower Farming

Modern floriculture faces unprecedented challenges in meeting the rising global demand for flowers while addressing environmental concerns and the need for sustainability. This paper explores the transformative role of biotechnology in revolutionizing flower farming practices, offering solutions for sustainable and resilient cultivation. The integration of biotechnological tools such as genetic engineering, tissue culture, and molecular breeding techniques has opened new avenues for enhancing the efficiency and resilience of flower crops. Genetic modification allows the development of varieties with improved traits, such as disease resistance, longer vase life, and enhanced stress tolerance. Tissue culture facilitates the rapid propagation of elite plant material, ensuring consistent and high-quality flower production. Furthermore, molecular breeding enables the precise selection of desirable traits, accelerating the traditional breeding process. The use of molecular markers for disease resistance, flower colour, and fragrance can streamline the development of new flower varieties tailored to market preferences. Additionally, advanced omics technologies, including genomics, transcriptomic, and metabolomics, provide valuable insights into the molecular mechanisms underlying flower development and responses to environmental stress. Biotechnological interventions extend beyond genetic improvements, encompassing sustainable cultivation practices. Precision agriculture, enabled by sensor technologies and data analytics, optimizes resource utilization, minimizing environmental impact. Smart irrigation systems, nutrient management, and pest control strategies contribute to the efficient use of resources and reduced ecological footprint. The biotechnological frontier in modern floriculture not only addresses current challenges but also positions the industry to adapt to future uncertainties. Climate change, emerging pests, and shifting market demands necessitate resilient flower farming systems. Biotechnology offers the tools to develop adaptable flower varieties and sustainable production methods, promoting long-term ecological and economic viability.

Enhancing the safety of CAR-T cell therapy: Synthetic genetic switch for spatiotemporal control.

Chimeric antigen receptor T (CAR-T) cell therapy is a promising and precise targeted therapy for cancer that has demonstrated notable potential in clinical applications. However, severe adverse effects limit the clinical application of this therapy and are mainly caused by uncontrollable activation of CAR-T cells, including excessive immune response activation due to unregulated CAR-T cell action time, as well as toxicity resulting from improper spatial localization. Therefore, to enhance controllability and safety, a control module for CAR-T cells is proposed. Synthetic biology based on genetic engineering techniques is being used to construct artificial cells or organisms for specific purposes. This approach has been explored in recent years as a means of achieving controllability in CAR-T cell therapy. In this review, we summarize the recent advances in synthetic biology methods used to address the major adverse effects of CAR-T cell therapy in both the temporal and spatial dimensions.

Advancements in Genetic Engineering for Enhanced Traits in Horticulture Crops: A Comprehensive Review

Genetic engineering has emerged as a powerful tool in the field of horticulture to enhance the traits of crops, leading to improved yield, quality, and resistance to biotic and abiotic stresses. This comprehensive review explores the recent advancements in genetic engineering techniques applied to horticulture crops, providing a detailed overview of the innovative strategies employed to manipulate plant genomes. The review begins by discussing the evolution of genetic engineering in horticulture, highlighting key milestones and breakthroughs that have paved the way for current advancements. It covers a range of techniques such as CRISPR-Cas9, RNA interference, and synthetic biology, shedding light on their applications in modifying specific genes responsible for desired traits. The main focus of the review is on the enhancement of key horticultural traits, including disease resistance, pest resistance, abiotic stress tolerance, and post-harvest attributes. Examples from various horticultural crops, such as fruits, vegetables, and ornamental plants, illustrate the success stories and potential applications of genetic engineering in each category. The ethical and regulatory aspects of genetic engineering in horticulture are also explored, addressing concerns related to environmental impact, biodiversity, and consumer acceptance. The review emphasizes the importance of responsible and sustainable practices in the application of genetic engineering to ensure the long-term benefits without adverse consequences. Furthermore, the review delves into emerging trends and future prospects in the field, including the potential of genome editing for precision breeding, the use of omics technologies for targeted trait improvements, and the integration of genetic engineering with other breeding techniques. It also discusses the challenges and opportunities associated with the global adoption of genetically modified horticulture crops.