Bioinformatics has gradually received widespread attention and has shown the characteristics of a large amount of calculation and high complexity. Therefore, it is required to adopt computer algorithms in bioinformatics to improve the efficiency of bioinformatics processing problems. Big data and artificial intelligence technologies have the characteristics of supporting bioinformatics and have achieved certain results in the field of bioinformatics. Introduced the application basis of big data and artificial intelligence in bioinformatics, analyzed data collection, preprocessing, data storage and management, data analysis, and mining technology. Furthermore, typical applications in bioinformatics are discussed in terms of gene expression data analysis, genome sequence information analysis, biological sequence difference and similarity analysis, genetic data analysis, and protein structure and function prediction. Finally, the bottlenecks and challenges in the application of big data and artificial intelligence in bioinformatics are discussed, and the application prospects of related technologies in bioinformatics have prospected.