General Mode Research Articles

Design and Motion Planning of a Cable Robot Utilizing Cable Slackness

Abstract Extending the workspace of cable robots can enhance their motion ability and help us make fuller use of their potential. This work designs a novel cable robot and utilizes cable slackness to extend its workspace. First, we use driving cables actuated by main motors and constrained cables driven by auxiliary motors equipped with lockers to control and restrain the motion of the end-effector, respectively. Then we classify the constraints on the end-effector into different modes according to the slackness of different constrained cables whose lengths can be changed by auxiliary motors, and continuous tension distribution (realized by tensioning devices) is applied to switch among them. To expand the mechanism’s workspace, the properties of the constraint mode series are analyzed and the static reachable workspace associated with it is defined, which guarantees the existence of feasible point-to-point trajectories. In particular, tree-based basic and general constraint mode series connection algorithms are designed to connect two targets in the static reachable workspace efficiently considering the necessary condition for the existence of the connection. After that, dual workspaces and subspace-based motion planning algorithms are designed to connect multiple targets in the static reachable workspace of the mechanism, and the quality of the trajectories is improved by using different manipulations of the constraint mode series. Since only driving cables are actuated by real-time capable servo motors and passively constrained cables are adopted to compensate for the weight of the end-effector, the size and the number of actuators and the energy consumption of the robot are reduced. Finally, the performance of the mechanism designed and the motion planning methods proposed are verified numerically under practical modeling errors regarding the cable elongation phenomenon.

Modeling the Electrical Stabilities of Organic Light‐Emitting Diodes

The ratio (R) of the molecular density of emissive layer to the carrier density in exciton formation zone has been provided to weigh the electrical stability of organic light‐emitting diode and simulated via the general mode of carrier device lifetimes. With current density increasing from 1 to 500 mA cm−2, the molecular density of emissive layer enabling the largest R, that is, the best electrical stability of device, gradually increases from 7.5 × 1019 to 1.5 × 1021 cm−3. At a given current density, R increases with the average bandgap of emissive layer increasing. There is a power law dependence of R on current density. For host–guest emissive layer, the electrical stability of device decreases with guest concentration increasing, mostly because the average bandgap of the host–guest emissive layer decreases. The current research provides some deep insights into developing the emissive layers of organic light‐emitting diodes toward high luminance applications.

Experimental and numerical investigation of low‐velocity impact behavior and failure mode of shear deficient RC beam strengthened with CFRP strips

AbstractIt is well‐known that shear failure is a collapse mechanism that is the riskiest, fastest, and catastrophic and occurs with no visible signs of damage or prior warning for RC beams. Therefore, to prevent brittle shear failure, the RC beams should include sufficient shear reinforcement, such as stirrups, and be designed to have sufficient shear capacity. However, the RC beams' shear capacity becomes inadequate for various reasons. One of these reasons may be the acting of the impulsive impact load, which is uncommon and disregarded in the design phase on the RC beams. An experimental program was conducted to examine the impact behavior and failure mode of shear‐deficient RC beams in the scope of the present study. Besides, it aims to investigate the effectiveness of the strengthening method using CFRP strips in improving the general behavior, failure mode, and performance of shear‐deficient RC beams exposed to impact load. The time histories of the accelerations, displacements, impact loads, and strains in the CFRP strips were measured. They were interpreted how they are affected by experimental variables examined in the experimental study. Furthermore, the finite element model of the specimens was generated in the LS‐DYNA software, and the experimental and numerical results were compared by performing finite element analysis in terms of failure modes and general behavior.

Teachers' Perceptions in Using YouTube to Teach Listening in ESL Classroom

This study was centered around recognizing the impact of YouTube recordings as a listening learning media. It is accepted that the general media mode might work on students' ability to listen and make listening seriously intriguing. Although past research has been conducted to discuss the implementation of YouTube videos in ESL classrooms, not much research has been done that focuses on the teachers' perception of using YouTube for listening skills. The objectives of the study are to identify how frequently ESL educators at the secondary level use YouTube recordings in their classroom for listening practices and to find out how much YouTube recordings provide ESL students with new techniques that can assist them with practicing their listening ability. The researchers employed a survey method design and chose 30 respondents using purposive sampling. Observations field notes were also employed to triangulate the data. The results of the study revealed that the teachers feel that the use of YouTube to teach listening in ESL classrooms is efficient and effective. They also stated that the school facilities should be sufficient to conduct an effective listening lesson using YouTube videos.

Derivation of an effective plate theory for parallelogram origami from bar and hinge elasticity

Periodic origami patterns made with repeating unit cells of creases and panels bend and twist in complex ways. In principle, such soft modes of deformation admit a simplified asymptotic description in the limit of a large number of cells. Starting from a bar and hinge model for the elastic energy of a generic four parallelogram panel origami pattern, we derive a complete set of geometric compatibility conditions identifying the pattern’s soft modes in this limit. The compatibility equations form a system of partial differential equations constraining the actuation of the origami’s creases (a scalar angle field) and the relative rotations of its unit cells (a pair of skew tensor fields). We show that every solution of the compatibility equations is the limit of a sequence of soft modes — origami deformations with finite bending energy and negligible stretching. Using these sequences, we derive a plate-like theory for parallelogram origami patterns with an explicit coarse-grained quadratic energy depending on the gradient of the crease-actuation and the relative rotations of the cells. Finally, we illustrate our theory in the context of two well-known origami designs: the Miura and Eggbox patterns. Though these patterns are distinguished in their anticlastic and synclastic bending responses, they show a universal twisting response. General soft modes captured by our theory involve a rich nonlinear interplay between actuation, bending and twisting, determined by the underlying crease geometry.

Conceptual design and experimental investigation of regolith bag structures for lunar in situ construction

Lunar habitat construction is a critical engineering requirement in lunar exploration missions, characterized by unique structural and material challenges distinct from Earth. To meet the demands of in situ large-scale construction in extreme lunar environments, a general mode of “Prefabricated core module & Inflatable structure &In situ materials structure” is adopted. This work analyzed the structural internal forces and foundation bearing capacity for lunar habitats, highlighting the significance of structural tensile performance and the foundation's allowable bearing capacity. A construction scheme based on regolith bags is proposed, encompassing architectural and structural design, and long-term planning. Multiscale experimental investigations conducted on “material, component, structure, and construction” levels confirm the excellent properties of regolith bag. Aramid fabric and CUG lunar regolith simulant were chosen for material and component testing, focusing on evaluating the regolith bag's performance under tension and compression. Successful construction demonstrations of a regolith bag arch structure and a regolith bag – airbag structural unit validate the feasibility of this construction scheme. Conclusively, the proposed lunar habitat construction scheme based on regolith bag technology provides a viable and efficient method for in situ lunar construction.

Control of G protein–coupled receptor function via membrane-interacting intrinsically disordered C-terminal domains

G protein-coupled receptors (GPCRs) control intracellular signaling cascades via agonist-dependent coupling to intracellular transducers including heterotrimeric G proteins, GPCR kinases (GRKs), and arrestins. In addition to their critical interactions with the transmembrane core of active GPCRs, all three classes of transducers have also been reported to interact with receptor C-terminal domains (CTDs). An underexplored aspect of GPCR CTDs is their possible role as lipid sensors given their proximity to the membrane. CTD-membrane interactions have the potential to control the accessibility of key regulatory CTD residues to downstream effectors and transducers. Here, we report that the CTDs of two closely related family C GPCRs, metabotropic glutamate receptor 2 (mGluR2) and mGluR3, bind to membranes and that this interaction can regulate receptor function. We first characterize CTD structure with NMR spectroscopy, revealing lipid composition-dependent modes of membrane binding. Using molecular dynamics simulations and structure-guided mutagenesis, we then identify key conserved residues and cancer-associated mutations that modulate CTD-membrane binding. Finally, we provide evidence that mGluR3 transducer coupling is controlled by CTD-membrane interactions in live cells, which may be subject to regulation by CTD phosphorylation and changes in membrane composition. This work reveals an additional mechanism of GPCR modulation, suggesting that CTD-membrane binding may be a general regulatory mode throughout the broad GPCR superfamily.

Acoustic design evaluation in educational buildings using artificial intelligence

Speech intelligibility is a critical aspect of building science, particularly in educational buildings where poor sound quality may have a detrimental impact on students' learning and teachers’ health. However, considering the numerous building regulations proposing varying definitions and ranges of acoustic comfort, calculating the necessary acoustic indicators can be challenging for designers. Speech intelligibility is a crucial component of indoor acoustics and acoustic comfort and can be calculated using formulas, simulation software, and data-based web tools. While formulas are fast, they lack details; acoustic simulation software is highly accurate but time-consuming and expensive. Data-based web tools, including machine learning algorithms, offer both speed and accuracy and are widely accessible. In this study, we present a system utilizing machine learning techniques to predict acoustic indicators, numeric and heatmap, in an educational building. The Pachyderm plugin in the Grasshopper was utilized to conduct extensive simulations in a single educational space, focusing on acoustic indicators in six different frequencies and general modes. Using Catboost and the pix2pix algorithm, the prediction models provide numerical and image indices on the developed dataset. Also, SHAP values were employed to interpret the Catboost model, analyzing the significance of each feature. The results showed remarkable accuracy, (i.e., between 89 % and 99 %) in the numerical portion, and PSNR index ranging from 0.817 to 0.970, and an SSIM index ranging from 15.56 to 31.57 in the image section. By utilizing data-driven methods, the system provides an efficient and accurate approach to calculating acoustic indicators, helping to ensure optimal acoustic environment in educational buildings.

A New Age of Biocatalysis Enabled by Generic Activation Modes

A New Age of Biocatalysis Enabled by Generic Activation Modes

The Significance of Processing Techniques on the Quality of Smoked African Cat Fish (Clarias gariepinus) Using Different Energy Sources

There is unavoidable need for fish smoking as a preservative technique to reduce rampant post harvest losses in the tropical Africa. However the imperative of healthier and quality smoked products through improved processing method were however investigated. Organoleptic and chemical assessment of African catfish collected from a reputable fish farm in Ogbomoso, Oyo state Nigeria was carried out. Eviscerated and non-eviserated fish samples were smoked using four different smoke sources: Gmelina (Gmnelina arborea), Parkia (Parkia biglobosa), Neem (Azadirachita indica) and charcoal. Overall acceptability was based on 5-point Hedonic scale (excellent (5) - poor (1). Chemical test was on Polycyclic Aromatic Hydrocarbon contents (benzo(a)pyrene, Fluoranthene, Napthalene, Acenapthalene, Chrysene and Benzo(ghi) perylene). Data were subjected to one way Analysis of Variance (ANOVA) using general linear mode (GLM) of Factorial Experimental Design and means were separated by Duncan’s multiple range tests (P<0.05). Non- eviscerated smoked African catfish samples had lower scores for colour than eviscerated smoked samples for energy sources used. Eviscerated samples had mean scores of 4.64, 3.91, 3.36 and 2.82 for charcoal, Gmelina, Neem and Parkia smoked samples respectively, much better than scores of 4.36, 2.46, 2.36 and 2.27 obtained for non-eviscerated samples. Total concentrations of polycyclic aromatic hydrocarbons (PAH) were highest in samples produced using Gmelina as smoke source and Neem for eviscerated (15.25 µg/kg) and non-eviscerated (29.43 µg/kg) samples respectively. Evisceration of fish prior to smoking enhances its sensory characteristics however; concentrations of PAHs for both eviscerated and non-eviscerated fish samples were within the recommended international standard limit.

Modeling the cortical response elicited by wrist manipulation via a nonlinear delay differential embedding.

Regarding motor processes, modeling healthy people's brains is essential to understand the brain activity in people with motor impairments. However, little research has been undertaken when external forces disturb limbs, having limited information on physiological pathways. Therefore, in this paper, a nonlinear delay differential embedding model is used to estimate the brain response elicited by externally controlled wrist movement in healthy individuals. The aim is to improve the understanding of the relationship between a controlled wrist movement and the generated cortical activity of healthy people, helping to disclose the underlying mechanisms and physiological relationships involved in the motor event. To evaluate the model, a public database from the Delft University of Technology is used, which contains electroencephalographic recordings of ten healthy subjects while wrist movement was externally provoked by a robotic system. In this work, the cortical response related to movement is identified via Independent Component Analysis and estimated based on a nonlinear delay differential embedding model. After a cross-validation analysis, the model performance reaches 90.21% ± 4.46% Variance Accounted For, and Correlation 95.14% ± 2.31%. The proposed methodology allows to select the model degree, to estimate a general predominant operation mode of the cortical response elicited by wrist movement. The obtained results revealed two facts that had not previously been reported: the movement's acceleration affects the cortical response, and a common delayed activity is shared among subjects. Going forward, identifying biomarkers related to motor tasks could aid in the evaluation of rehabilitation treatments for patients with upper limbs motor impairments.

Clinical data analysis of 117 patients with ruptured abdominal aortic aneurysm

Objective: To examine the perioperative clinical features and prognosis of patients with ruptured abdominal aortic aneurysms (rAAA) who received surgical repair. Methods: The clinical data of rAAA patients who underwent surgical repair and were admitted to the Surgical Intensive Care Unit of Beijing Anzhen Hospital, Capital Medical University from August 2005 to November 2020 were retrospectively analyzed, including the general clinical features, surgical mode, intraoperative conditions, postoperative complications, and fatality rate. Results: There were 117 patients with rAAA, with a median age of 68 (62,77) years, including 93 men (79.5%) and 24 women (20.5%). The main clinical manifestation was abdominal pain (n=115, 98.3%). Among them, 65 (55.6%) patients underwent endovascular aneurysm repair (EVAR), while 52 (44.4%) underwent open surgical repair (OSR). The common postoperative complications include acute gastrointestinal dysfunction (n=116, 99.1%), shock (n=89, 76.1%), acute respiratory distress syndrome (n=85, 72.6%), pancreatic injury (n=56, 47.9%), coagulation dysfunction (n=55, 47.0%), disseminated intravascular coagulation (n=46, 39.3%), acute kidney injury (n=39, 33.3%), infection/sepsis (n=28, 23.9%), gastrointestinal bleeding (n=17, 14.5%), and abdominal compartment syndrome (n=12, 10.3%). The overall postoperative in-hospital fatality rate was 10.3% (12/117). Preoperative use of vasopressors and inotropes, retroperitoneal hematoma, and postoperative abdominal compartment syndrome, gastrointestinal hemorrhage, acute kidney injury, and diffuse intravascular coagulation significantly increased the fatality rate [5/11, 6/24, 5/16, 6/12, 6/17, 23.1%(9/39), 19.6%(9/46), respectively]. Conclusion: The postoperative mortality of rAAA patients is still high in the era of EVAR, especially in patients with preoperative existence of shock and retroperitoneal hematoma, and with postoperative abdominal compartment syndrome, coagulation dysfunction, and acute kidney injury. It is necessary to strengthen perioperative monitoring and management of these patients to reduce the fatality rate.

Evolutionary Game Analysis on Cooperative Behavior of Major Projects’ Technology Innovation Subjects under General Contracting Mode

Major projects are the important platform for enhancing a country’s comprehensive national power and strengthening its capacity for independent innovation. Although major projects in China have made remarkable achievements, willingness to cooperate and innovate has not achieved the desired target. In this paper, the evolutionary game model of cooperative innovation behavior of general contractors and subcontractors is constructed by considering reputational factors. Through theoretical derivation, the influence of the distribution ratio of collaborative innovation benefit, spillover technology absorption capacity, and reputation discounting coefficient on innovation behavior is analyzed. Finally, MATLAB software is used to simulate the dynamic evolution process of strategy selection. The results show that (1) a reasonable benefit distribution coefficient can promote the evolution of innovation behavior in a positive direction; (2) both the reduction of innovation cost and the increase of spillover technology absorption capacity can make the innovation subject more inclined to choose the active collaborative innovation strategy; and (3) it is the higher-than-threshold reputation loss that can effectively inhibit the “free-rider” behavior. The research conclusions and managerial implications can provide reference for improving the willingness to cooperate in major projects’ technology innovation.

Project ChemicalBlooms: Collaborating with citizen scientists to survey the chemical diversity and phylogenetic distribution of plant epicuticular wax blooms.

Plants use chemistry to overcome diverse challenges. A particularly striking chemical trait that some plants possess is the ability to synthesize massive amounts of epicuticular wax that accumulates on the plant's surfaces as a white coating visible to the naked eye. The ability to synthesize basic wax molecules appears to be shared among virtually all land plants, and our knowledge of ubiquitous wax compound synthesis is reasonably advanced. However, the ability to synthesize thick layers of visible epicuticular crystals ("wax blooms") is restricted to specific lineages, and our knowledge of how wax blooms differ from ubiquitous wax layers is less developed. Here, we recruited the help of citizen scientists and middle school students to survey the wax bloom chemistry of 78 species spanning dicot, monocot, and gymnosperm lineages. Using gas chromatography-mass spectrometry, we found that the major wax classes reported from bulk wax mixtures can be present in wax bloom crystals, with fatty acids, fatty alcohols, and alkanes being present in many species' bloom crystals. In contrast, other compounds including aldehydes, ketones, secondary alcohols, and triterpenoids were present in only a few species' wax bloom crystals. By mapping the 78 wax bloom chemical profiles onto a phylogeny and using phylogenetic comparative analyses, we found that secondary alcohol and triterpenoid-rich wax blooms were present in lineage-specific patterns that would not be expected to arise by chance. That finding is consistent with reports that secondary alcohol biosynthesis enzymes are found only in certain lineages but was a surprise for triterpenoids, which are intracellular components in virtually all plant lineages. Thus, our data suggest that a lineage-specific mechanism other than biosynthesis exists that enables select species to generate triterpenoid-rich surface wax crystals. Overall, our study outlines a general mode in which research scientists can collaborate with citizen scientists as well as middle and high school classrooms not only to enhance data collection and generate testable hypotheses but also to directly involve classrooms in the scientific process and inspire future STEM workers.

Review on the technical catch-up paradigm

Technological innovation plays an increasingly prominent role in the process of economic development, and it should be based on technological innovation in developing the catch-up strategy. On the basis of sorting out the existing technology catch-up path, and according to some cases of domestic and foreign technology catch-up, the general mode of late-development enterprises and countries in the process of catch-up technology catch-up, that is, early jump-middle follow-later creation. The whole catch-up process should take learning, digesting and introducing technology, improving technical ability and enhancing independent innovation ability as the main line. Whether the path jumping, path following or path creation technology catch-up path can only become part of the whole catch-up process.

Donor Preactivation-Based Glycan Assembly: from Manual to Automated Synthesis.

Carbohydrates are called the third chain of life. Carbohydrates participate in many important biochemical functions in living species, and the biological information carried by them is several orders of magnitude larger than that of nucleic acids and proteins. However, due to the intrinsic complexity and heterogeneity of carbohydrate structures, furnishing pure and structurally well-defined glycans for functional studies is a formidable task, especially for homogeneous large-size glycans. To address this issue, we have developed a donor preactivation-based one-pot glycosylation strategy enabling multiple sequential glycosylations in a single reaction vessel.The donor preactivation-based one-pot glycosylation refers to the strategy in which the glycosyl donor is activated in the absence of a glycosyl acceptor to generate a reactive intermediate. Subsequently, the glycosyl acceptor with the same anomeric leaving group is added, leading to a glycosyl coupling reaction, which is then iterated to rapidly achieve the desired glycan in the same reactor. The advantages of this strategy include the following: (1) unique chemoselectivity is obtained after preactivation; (2) it is independent of the reactivity of glycosyl donors; (3) multiple-step glycosylations are enabled without the need for intermediate purification; (4) only stoichiometric building blocks are required without complex protecting group manipulations. Using this protocol, a range of glycans including tumor-associated carbohydrate antigens, various glycosaminoglycans, complex N-glycans, and diverse bacterial glycans have been synthesized manually. Gratifyingly, the synthesis of mycobacterial arabinogalactan containing 92 monosaccharide units has been achieved, which created a precedent in the field of polysaccharide synthesis. Recently, the synthesis of a highly branched arabinogalactan from traditional Chinese medicine featuring 140 monosaccharide units has been also accomplished to evaluate its anti-pancreatic-cancer activity. In the spirit of green and sustainable chemistry, this strategy can also be applied to light-driven glycosylation reactions, where either UV or visible light can be used for the activation of glycosyl donors.Automated synthesis is an advanced approach to the construction of complex glycans. Based on the two preactivation modes (general promoter activation mode and light-induced activation mode), a universal and highly efficient automated solution-phase synthesizer was further developed to drive glycan assembly from manual to automated synthesis. Using this synthesizer, a library of oligosaccharides covering various glycoforms and glycosidic linkages was assembled rapidly, either in a general promoter-activation mode or in a light-induced-activation mode. The automated synthesis of a fully protected fondaparinux pentasaccharide was realized on a gram scale. Furthermore, the automated synthesis of large-size polysaccharides was performed, allowing the assembly of arabinans up to an astonishing 1080-mer using the automated multiplicative synthesis strategy, taking glycan synthesis to a new height far beyond the synthesis of nucleic acids (up to 200-mer) and proteins (up to 472-mer).

PEMBANGUNAN HUKUM DAGANG MENUJU INDONESIA EMAS

On the development of the legal system is of course inseparable from the development of other fields, because the development of the legal system is part of the country's development. Legal development must be integrated and coordinated with the development agenda in other fields such as commercial law, which requires a continuous process. Currently, legal developments also need to pay attention to world developments. Here a clear strategy is needed so that legal developments can be harmonized with global developments without abandoning the values of the Indonesian nation. The purpose of this article is to examine the needs for the development of Indonesian commercial law and find a model of trade law reform that we want so that it can meet the needs and support the country's development. The approach taken in this paper is to standardize justice and collect secondary data sources through library research. Qualitative analysis of the collected data. Several provisions of the Criminal Code are outdated, and the related field of law is developing rapidly, which is no longer able to meet the current needs of trade law. Updating Indonesian trade law needs to be carried out immediately to meet developments, the needs of the national community and international demands. There are many modes to choose from for updating Indonesian trade law, including general codification mode, partial codification mode, statutory regulation preparation mode, open codification mode and partial regulation of Indonesian trade law. As trade law develops, it is believed that this will support Indonesia's vision of gold in the future.

Fundamental research on the condensation heat transfer of the hydrocarbon-mixture energy in a spiral tube described by a universal model using flow pattern based and general modes

The transportation of liquefied natural gas (LNG) is a crucial aspect of global energy application and security. However, there are still some challenges in liquefaction. This study establishes systematic experiments to investigate the condensation two-phase flow and heat transfer process of hydrocarbon-mixture energy (methane/ethane/propane/isobutane) in a spiral tube. The main influencing parameters, including mass flux (200–560 kg/(m2·s)), operating pressure (2–4 MPa), and vapor quality (0–1) on the heat transfer intensity and frictional pressure drop were analyzed. Different two-phase flow patterns were observed and categorized them as bubble flow, intermittent flow, stratified-wavy flow, and annular flow. For bubble flow and intermittent flow, vapor quality is found to remarkably affect the heat transfer; regarding stratified-wavy flow and annular flow, gravitational force and inertia force are found to prominently influence the heat transfer, respectively. Additionally, a newly universal model includes split flow patterns mode (SFPM) and the general mode (GM) has been proposed, which is with high-precision for describing the condensation heat transfer coefficients of different flow patterns. Furthermore, the analytical results show the SFPM's root mean square error and mean absolute relative deviation are 97.35 W/(m2·K) and 0.036.

Principle and application of holographic modal wavefront sensor for extended objects and general aberration modes

By employing a computer-generated hologram (CGH) to modulate the incident wavefront, the holographic modal wavefront sensor (HMWFS) can efficiently measure multiple aberration modes in a single shot, providing significant advantages such as computational simplicity, rapid measurement speed, and customizable assessment of aberration modes. However, the current HMWFS is confined to point-like objects and aberrations with specific orthogonality, thereby limiting its application scope. In this paper, we introduce a novel HMWFS that, to the best of our knowledge, is compatible with extended objects and general aberration modes. We establish the theoretical relationship between the second moment of the image intensity and the coefficients of general aberration modes for extended objects. Simulation and experimental results confirm the feasibility and performance of the proposed HMWFS. Additionally, we demonstrated its practical use in measuring misalignments of the secondary mirror of a space telescope. Simulations show that the misalignments can be effectively corrected within 2∼3 iterations.

Standard methods to estimate strength parameters, flight activity, comb construction, and fitness of Apis mellifera colonies 2.0

This paper describes methods for estimating honey bee (Apis mellifera) colony strength by which mean population measures of adult bees and brood are obtained. Additionally, secondary measures, such as the quantity of stored honey and pollen, brood pattern, flight activity, comb construction, and the expression of visible disease or parasite symptoms are addressed. There are generally two contexts in which an investigator wishes to measure colony strength: (1) at the beginning of a study as part of manipulations to produce uniform colonies and reduce experimental error, and (2) as a response variable during or at the end of an experiment. Moreover, there are two general modes for measuring colony strength: (1) an objective mode that uses quantitative measures, and (2) a subjective mode that relies on visual estimates by one or more observers. Other parameters that do not directly measure colony strength are described because they give important indicators of colony state. These parameters include flight activity at the hive entrance, comb construction, and two proxy measures of colony fitness: production of queen cells and drone brood.