General Maintenance Research Articles

Does time matter? Intraspecific diversity of ribosomal RNA genes in lineages of the allopolyploid model grass Brachypodium hybridum with different evolutionary ages

BackgroundPolyploidisation often results in genome rearrangements that may involve changes in both the single-copy sequences and the repetitive genome fraction. In this study, we performed a comprehensive comparative analysis of repetitive DNA, with a particular focus on ribosomal DNA (rDNA), in Brachypodium hybridum (2n = 4x = 30, subgenome composition DDSS), an allotetraploid resulting from a natural cross between two diploid species that resemble the modern B. distachyon (2n = 10; DD) and B. stacei (2n = 20; SS). Taking advantage of the recurrent origin of B. hybridum, we investigated two genotypes, Bhyb26 and ABR113, differing markedly in their evolutionary age (1.4 and 0.14 Mya, respectively) and which resulted from opposite cross directions. To identify the origin of rDNA loci we employed cytogenetic and molecular methods (FISH, gCAPS and Southern hybridisation), phylogenetic and genomic approaches.ResultsUnlike the general maintenance of doubled gene dosage in B. hybridum, the rRNA genes showed a remarkable tendency towards diploidisation at both locus and unit levels. While the partial elimination of 35S rDNA units occurred in the younger ABR113 lineage, unidirectional elimination of the entire locus was observed in the older Bhyb26 lineage. Additionally, a novel 5S rDNA family was amplified in Bhyb26 replacing the parental units. The 35S and 5S rDNA units were preferentially eliminated from the S- and D-subgenome, respectively. Thus, in the more ancient B. hybridum lineage, Bhyb26, 5S and 35S rRNA genes are likely expressed from different subgenomes, highlighting the complexity of polyploid regulatory networks.ConclusionComparative analyses between two B. hybridum lineages of distinct evolutionary ages revealed that although the recent lineage ABR113 exhibited an additive pattern of rDNA loci distribution, the ancient lineage Bhyb26 demonstrated a pronounced tendency toward diploidisation manifested by the reduction in the number of both 35S and 5S loci. In conclusion, the age of the allopolyploid appears to be a decisive factor in rDNA turnover in B. hybridum.

The Role of the Civil Service Police Unit in Public Order and Community Peace

The general background maintenance of public order and community peace is a critical aspect of local governance, particularly in urban settings where diverse populations coexist. Specific background reveals that the Civil Service Police Unit (Satpol PP) plays a significant role in upholding these values, yet there is limited research examining their effectiveness in specific districts. Knowledge gap persists in understanding the comprehensive functions and impacts of Satpol PP in maintaining public order within local communities. This study aims to analyze and describe the role of the Satpol PP in implementing public order and community peace in a specific district. Results indicate that the Satpol PP engages in various activities, including community outreach, training for local volunteers, and proactive measures to prevent disturbances, demonstrating a multi-faceted approach to their responsibilities. Novelty lies in the identification of active, participatory, and passive roles that the Satpol PP adopts, which offers a more nuanced understanding of their functions. Implications of this research suggest that strengthening the capacity of the Satpol PP could enhance community engagement and further improve public safety. By providing a detailed examination of their roles, this study contributes to the broader discourse on local governance and community development, offering insights that may inform policy and practice in similar contexts. Highlights: Active role in maintaining public order through various strategies. Community participation in reporting issues to the Satpol PP. Training programs to enhance the capabilities of Linmas and Satpol PP personnel. Keywords: Satpol, Community, Public Order, Participation, Capacity Building

Condition-based Predictive Maintenance as an Efficient Strategy for Industrializing Additive Manufacturing Technology

Objective: Traditional manufacturing has several issues to determine a general maintenance strategy. Additive Manufacturing (AM) is a special type of fabrication, which possesses different issues, especially uncertainty failure cases. This forms a big obstacle to becoming an industrialized production strategy. The objective of this work is to provide the most suitable maintenance strategy in order to reduce the likelihood of failure that can help in industrializing the AM technology. Methods: Among the different maintenance strategies, Predictive Maintenance (PdM) became widely treated in academia and industry. According to the methods used for detecting the failure signs, it can be classified into two types: Condition-Based Predictive Maintenance (CBPdM) and Statistical-Based Predictive Maintenance (SBPdM). The use of the last type highly depends on available and accessible data. However, CBPdM depends on only periodic or continuous condition monitoring tools for detecting the failure signs. Results: The existence of various types of failure cases yields to a big difficulty to predict failures. In addition, because of insufficient data, SBPdM cannot be selected as a suitable maintenance strategy for additive manufacturing. According to the presented examples, the CBPdM can be considered here as the best maintenance strategy for AM. Conclusion: CBPdM strategy is the best compromise between cost and applicability where there is not enough data. This selected maintenance approach represents an efficient tool in industrializing AM technology.

The GC-content at the 5′ ends of human protein-coding genes is undergoing mutational decay

BackgroundIn vertebrates, most protein-coding genes have a peak of GC-content near their 5′ transcriptional start site (TSS). This feature promotes both the efficient nuclear export and translation of mRNAs. Despite the importance of GC-content for RNA metabolism, its general features, origin, and maintenance remain mysterious. We investigate the evolutionary forces shaping GC-content at the transcriptional start site (TSS) of genes through both comparative genomic analysis of nucleotide substitution rates between different species and by examining human de novo mutations.ResultsOur data suggests that GC-peaks at TSSs were present in the last common ancestor of amniotes, and likely that of vertebrates. We observe that in apes and rodents, where recombination is directed away from TSSs by PRDM9, GC-content at the 5′ end of protein-coding gene is currently undergoing mutational decay. In canids, which lack PRDM9 and perform recombination at TSSs, GC-content at the 5′ end of protein-coding is increasing. We show that these patterns extend into the 5′ end of the open reading frame, thus impacting synonymous codon position choices.ConclusionsOur results indicate that the dynamics of this GC-peak in amniotes is largely shaped by historic patterns of recombination. Since decay of GC-content towards the mutation rate equilibrium is the default state for non-functional DNA, the observed decrease in GC-content at TSSs in apes and rodents indicates that the GC-peak is not being maintained by selection on most protein-coding genes in those species.

A staged diversity enhancement method for constrained multiobjective evolutionary optimization

Optimizing the convergence and diversity of solutions simultaneously under constraints is a challenge in solving constrained multiobjective optimization problems. In existing multiobjective optimization algorithms, general diversity maintenance mechanisms have difficulty determining all optimal solutions in discrete feasible regions. This paper proposes a staged constrained multiobjective optimization algorithm with a diversity enhancement method (SDEM), which can explore potential discrete feasible regions by retaining well-distributed offspring. Specifically, after solutions have converged to optimal feasible regions by niching-based constraint dominance in the early stage, the SDEM improves the diversity of solutions through a proposed diversity enhancement dominance principle in the mid-term. Finally, the optimize objective functions and constraints of all solutions are optimized under constraint dominance to balance convergence, diversity, and feasibility during the three stages. Experiments on four well-known test suites and six real-world case studies demonstrate that the SDEM is competitive with or comparable to seven state-of-the-art constrained multiobjective evolutionary algorithms.

Uncovering the Role and Labor of Volunteer Leaders within a Community-Led Urban Tree Planting Initiative

Urban forestry programs across North America rely on volunteers to lead urban forest stewardship activities and encourage participation in tree planting and general maintenance. We examined the leadership structure of a tree planting initiative (TPI) in the greater Philadelphia region to understand the role and labor contributions of volunteer leaders, and the current challenges of achieving equitable urban tree canopy within community-led TPIs. Using an online survey, we found that leaders contribute unpaid labor, time, and skills within the following categories: group organization and communication, tree-planting and event coordination, tree care and maintenance, and broader environmental education and advocacy. Volunteer leaders also face challenges related to leadership and administrative tasks. This study deepens understandings of the labor needs within volunteer TPIs and potential ways to support the equitable growth of these initiatives moving forward. Future research is needed to understand recognitional equity gaps in volunteer leadership more broadly.

Evaluation of Sustainable Maintenance and Security of Green Spaces in Public Housing Schemes in Lagos State, Nigeria

Security of lives and property in housing environments is one of the basic necessity in the provision of public housing. The nature of maintenance given to green spaces in public housing environment can affect the level of security of lives and property. However, due to the antecedents of public housing, its unique population and the problems it creates for law enforcement agencies on security issues, there is need to evaluate how sustainable maintenance of green spaces within public housing could affect the level of security of lives and property. The public housing constructed by the Lagos State Development and Property Corporation (LSDPC) in Lagos State, Nigeria was used for this study. Primary data was collected from 1036 housing units in 12 different estates of the LSDPC in Lagos State, using the research tools of the questionnaire and structured observation schedule. Descriptive statistics and categorical regression analyses were carried out on the data. The results showed that the green spaces around each housing units were sustainably maintained, than jointly owned green spaces by all residents of the housing estates and this has implications to the level of security of lives and property in the housing estates. It is recommended that public housing estate’s green spaces be designed to allow for sustainability in maintenance by each housing units, which would lead to a general sustainable maintenance view of all green spaces available in the public housing environment.

Optimal maintenance policies for three-unit parallel production systems considering yields

Many production facilities consist of multiple parallel units to facilitate the completion of production plans, yet these devices are inevitably prone to malfunctions with increased operating time. Therefore, developing the optimal maintenance policy to maximize the production capacity of production facilities is a crucial issue. This paper focuses on determining the optimal maintenance policy for a three-unit production system, aiming to maximize its yield. The optimal solution is provided based on the given maintenance policy, and a general optimal maintenance policy for the system is derived. The properties related to the optimal maintenance policy are analyzed. The proposed model offers two notable contributions when compared to existing research. Firstly, a maintenance policy based on the number of visits and yields is developed. Secondly, the parallel assumption of the system and the assumption that the subsystems satisfy any distribution are in line with reality, enriching the research on maintenance strategies for production systems. To illustrate the results of the model, a numerical example is presented.

Genomic deletion of Bcl6 differentially affects conventional dendritic cell subsets and compromises Tfh/Tfr/Th17 cell responses.

Conventional dendritic cells (cDC) play key roles in immune induction, but what drives their heterogeneity and functional specialization is still ill-defined. Here we show that cDC-specific deletion of the transcriptional repressor Bcl6 in mice alters the phenotype and transcriptome of cDC1 and cDC2, while their lineage identity is preserved. Bcl6-deficient cDC1 are diminished in the periphery but maintain their ability to cross-present antigen to CD8+ T cells, confirming general maintenance of this subset. Surprisingly, the absence of Bcl6 in cDC causes a complete loss of Notch2-dependent cDC2 in the spleen and intestinal lamina propria. DC-targeted Bcl6-deficient mice induced fewer T follicular helper cells despite a profound impact on T follicular regulatory cells in response to immunization and mounted diminished Th17 immunity to Citrobacter rodentium in the colon. Our findings establish Bcl6 as an essential transcription factor for subsets of cDC and add to our understanding of the transcriptional landscape underlying cDC heterogeneity.

Decreased Ubiquitination and Acetylation of Histones 3 and 4 Are Associated with Obesity-Induced Disorders of Spermatogenesis in Mice.

Obesity, a chronic metabolic disorder, is related to cardiovascular diseases, diabetes, cancer, and reproductive disorders. The relationship between obesity and male infertility is now well recognized, but the mechanisms involved are unclear. We aimed to observe the effect of obesity on spermatogenesis and to investigate the role of histone ubiquitination and acetylation modifications in obesity-induced spermatogenesis disorders. Thirty male C57BL/6J mice were randomly divided into two groups. The control group was fed with a general maintenance diet (12% fat), while a high-fat diet (HFD) group was fed with 40% fat for 10 weeks; then, they were mated with normal females. The fertility of male mice was calculated, testicular and sperm morphology were observed, and the expression levels of key genes and the levels of histone acetylation and ubiquitination modification during spermatogenesis were detected. The number of sperm was decreased, as well as the sperm motility, while the number of sperm with malformations was increased. In the testes, the mRNA and protein expression levels of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25), chromosome region maintenance-1 protein (CRM1), high-mobility group B2 (HMGB2), phosphoglycerate kinase 2 (PGK2), and testicular angiotensin-converting enzyme (tACE) were decreased. Furthermore, obesity led to a decrease in ubiquitinated H2A (ubH2A) and reduced levels of histone H3 acetylation K18 (H3AcK18) and histone H4 acetylation K5, K8, K12, and K16 (H4tetraAck), which disrupted protamine 1 (Prm1) deposition in testis tissue. These results suggest that low levels of histone ubiquitination and acetylation are linked with obesity-induced disorders during spermatogenesis, contributing to a better understanding of obesity-induced damage to male reproduction.

Clinical Application and Research Progress of Remimazolam for Pediatric Patients.

Remimazolam is a novel ultrashort-acting benzodiazepine that allosterically modulates γ-aminobutyric acid type A (GABAA) receptors to exert sedative effects. Remimazolam has the properties of controllable sedation, rapid onset, and a short duration of action, along with minor depression of circulation and respiration. Remimazolam has been approved for clinical use since 2020 in Japan, and it has been applied for procedural sedation, general anesthesia induction and maintenance, and sedation in ICU patients, and has been proven to be safe and effective. Currently, no consensus has been reached on the clinical application of remimazolam in pediatric patients. This review introduces the clinical research progress and limitations of remimazolam in recent years, aiming to supply scientific guidance and a theoretical reference for the application of remimazolam in pediatric anaesthesia.

Studies on the Benefits of Cogon Grass (Imperata cylindrica L) in Indonesia: A Review

Cogon grass (Imperata cylindrica L.) is one of the most invasive weeds in the world which causes many losses in agriculture and plantations. To find added value from cogon grass, in many countries in the world, including Indonesia, various studies have been carried out on the aspects that can be utilized from this grass. In this article, the results of a study of published research articles on cogon grass that have been carried out in Indonesia by Indonesian researchers are presented. The results showed that there were at least 48 research articles on the cogon grass found. There are five benefits of cogon grass were found, namely as a source of allelopathic ingredients for weed control, a source of pharmacological medicinal ingredients, general health maintenance ingredients, a source of animal feed, and a source of energy.

Strategi Pengembangan Bisnis Rest Area Silaing Bawah Kota Padang Panjang

The city of Padang Panjang has its own potential and uniqueness compared to other cities in West Sumatra. Supported by its beautiful natural potential, the City of Padang Panjang has several tourist attractions with quite good performance, including the Minangkabau Information and Culture Documentation Center (PDIKM), the Minang Fantasi Area (MIFAN). Providing a place to rest requires no small investment costs. This investment is needed to procure the basic infrastructure needed for resting places to be realized and operational. The basic infrastructure needed includes land, access roads, circulation, facilities, and utilities. In addition to investment in basic infrastructure procurement, operational costs are also required, which include costs for general expenditures, personnel expenditures, and maintenance expenditures. In order for the provision and management of rest areas to be sustainable, the planning and management of rest areas must be planned as efficiently and effectively as possible . The method used is qualitative or descriptive with a phenomenological approach with data triangulation. The results obtained are Based on the Cartesian SWOT diagram above, it is known that the lower Silaing rest area is in quadrant I, which means that the development strategy is SO, namely the strategy of using strengths to maximize existing opportunities. This strategy is also known as a growth oriented strategy, which is to support an aggressive growth policy. If you refer to this strategy, the Downtown Silaing Rest Area of Padang Panjang City improves the image of the rest area which makes the rest area get a good image from users.

Long-term Survival after Volatile or Propofol General Anesthesia for Bladder Cancer Surgery: A Retrospective National Registry Cohort Study.

Prospective interventional trials and retrospective observational analyses provide conflicting evidence regarding the relationship between propofol versus inhaled volatile general anesthesia and long-term survival after cancer surgery. Specifically, bladder cancer surgery lacks prospective clinical trial evidence. Data on bladder cancer surgery performed under general anesthesia between 2014 and 2021 from the National Quality Registry for Urinary Tract and Bladder Cancer and the Swedish Perioperative Registry were record-linked. Overall survival was compared between patients receiving propofol or inhaled volatile for anesthesia maintenance. The minimum clinically important difference was defined as a 5-percentage point difference in 5-yr survival. Of 7,571 subjects, 4,519 (59.7%) received an inhaled volatile anesthetic, and 3,052 (40.3%) received propofol for general anesthesia maintenance. The two groups were quite similar in most respects but differed in American Society of Anesthesiologists Physical Status and tumor stage. Propensity score matching was used to address treatment bias. Survival did not differ during follow-up (median, 45 months [interquartile range, 33 to 62 months]) in the full unmatched cohort nor after 1:1 propensity score matching (3,052 matched pairs). The Kaplan-Meier adjusted 5-yr survival rates in the matched cohort were 898 of 3,052, 67.5% (65.6 to 69.3%) for propofol and 852 of 3,052, 68.5% (66.7 to 70.4%) for inhaled volatile general anesthesia, respectively (hazard ratio, 1.05 [95% CI, 0.96 to 1.15]; P = 0.332). A sensitivity analysis restricted to 1,766 propensity score-matched pairs of patients who received only one general anesthetic during the study period did not demonstrate a difference in survival; Kaplan-Meier adjusted 5-yr survival rates were 521 of 1,766, 67.1% (64.7 to 69.7%) and 482 of 1,766, 68.9% (66.5 to 71.4%) for propofol and inhaled volatile general anesthesia, respectively (hazard ratio, 1.09 [95% CI, 0.97 to 1.23]; P = 0.139). Among patients undergoing bladder cancer surgery under general anesthesia, there was no statistically significant difference in long-term overall survival associated with the choice of propofol or an inhaled volatile maintenance.

Development of Sugarcane Bagasse Ash Blended Cementitious Composites Reinforced with Carbon Nanotubes and Polypropylene Fibers

Cement-based composites, as primary construction materials, have undergone significant advancements over the years, yet researchers still face challenges in terms of their durability and impact on the environment. The goal of this research is to develop environmentally friendly cementitious composites blended with sugarcane bagasse ash (SCBA) and reinforce them with multi-walled carbon nanotubes and polypropylene (PP) fibers. Because of the high cost associated with carbon nanotubes (CNTs) and PP fibers, as well as CO2 emission, which affect the economic and environmental aspects of this field, an agricultural waste such as SCBA was introduced in the current study that is both economically and environmentally viable. For this purpose, five mixes were designed by varying the CNTs content whilst keeping the PP fibers and SCBA contents constant at 1.5% and 15% by weight of the binder (ordinary Portland cement + SCBA), respectively. The developed blends were tested for various mechanical and durability properties, i.e., compressive strength, flexural strength, impact strength, water absorption, and ultrasonic pulse velocity. Moreover, the microstructures of the newly developed low-carbon SCBA-based composites reinforced with PP fibers and CNTs were studied through scanning electron microscopy and energy dispersive spectroscopy. The results showed that the developed blends incorporating 15% SCBA, 1.5% PP fibers, and 0.08% CNTs, by weight of the binder, demonstrated the compressive, flexural, and impact strengths as 15.30 MPa, 0.98 MPa, and 0.11 MPa, respectively. The investigated blends proved to be cost-effective and environmentally beneficial, rendering them suitable for utilization in general construction and maintenance works.

Prevalence and severity of design anti-patterns in open source programs—A large-scale study

Context:Design anti-patterns can be symptoms of problems that lead to long-term maintenance difficulty. How should development teams prioritize their treatment? Which ones are more severe and deserve more attention? Does the impact of anti-patterns and general maintenance efforts differ with different programming languages? Objective:In this study, we assess the prevalence and severity of anti-patterns in different programming languages and the impact of dynamic typing in Python, as well as the impact scopes of prevalent anti-patterns that manifest the violation of design principles. Method:We conducted a large-scale study of anti-patterns using 1717 open-source projects written in Java, C/C++, and Python. For the 288 Python projects, we extracted both explicit and dynamic dependencies and compared how the detected anti-patterns and maintenance costs changed. Finally, we removed anti-patterns involving five or fewer files to assess the impact of trivial anti-patterns. Results:The results reveal that 99.55% of these projects contain anti-patterns. Modularity Violation – frequent co-changes among seemingly unrelated files – is most prevalent (detected in 83.54% of all projects) and costly (incurred 61.55% of maintenance effort on average). Unstable Interface and Crossing, caused by influential but unstable files, although not as prevalent, tend to incur severe maintenance costs. Duck typing in Python incurs more anti-patterns, and the churn spent on Python files multiplies that of C/C++ and Java files. Several prevalent anti-patterns have a large portion of trivial instances, meaning that these common symptoms are usually not harmful. Conclusion:Implicit and visible dependencies are the most expensive to maintain, and dynamic typing in Python exacerbates the issue. Influential but unstable files need to be monitored and rectified early to prevent the accumulation of high maintenance costs. The violations of design principles are widespread, but many are not high-maintenance.

Francis Type Turbine Runner Design and Comparison with Model Test Results

Cavitation wear and hydraulic efficiency decrease in hydroelectric power plants have frequently been the subject of various research and studies. A hydroelectric power plant built on the Kızılırmak River in Türkiye started operating in 1960 and has not been subjected to any large-scale rehabilitation work other than general maintenance until today. The power plant has 4 Francis-type turbines, each with a power of 32 MW. Due to cavitation wear of turbine runners over the years, performance loss, vibration, and noise problems have arisen. Moreover, the maximum turbine hydraulic efficiency, which was 92% in 1960, the year the power plant was commissioned, decreased to 87.9% according to the efficiency measurements carried out at the power plant in 2020. In this study, Computational Fluid Dynamics (CFD) analyses were accomplished with Reynolds averaged Navier Stokes (RANS) calculations for the redesign of the Francis-type turbine runner and finally checked by a model test according to IEC 60193. It was observed that the model test and CFD results were close to each other, especially at the best efficiency point. The maximum turbine hydraulic efficiency, which was calculated as 94.95% as a result of CFD analysis at the nominal head, was calculated as 95.19% by the model test. The x-blade shape created in the redesigned turbine runner blades ensured homogeneous pressure distribution and increased the hydraulic efficiency significantly.

Postoperative pain after different doses of remifentanil infusion during anaesthesia: a meta-analysis

BackgroundThis meta-analysis aimed to explore the correlation between the different doses of remifentanil-based anaesthesia and postoperative pain in randomised trials.MethodsThe electronic databases including PubMed, Cochrane, clinical trial registries, and Google Scholar were searched up to November 2022 for randomised controlled trials (RCTs) that assessed the dose dependent efficacy of remifentanil for postoperative pain intensity and hyperalgesia.Results31 studies involving 2019 patients were included for analysis. Compared with the high remifentanil dose administration, patients in low doses showed less postoperative pain intensity at 1-2 h (weighted mean differences (WMD): 0.60, 95% CI, 0.05 to 1.15), 3-8 h (WMD: 0.38, 95% CI, 0.00 to 0.75), 24 h (WMD: 0.26, 95% CI, 0.04 to 0.48) and 48 h (WMD: 0.32, 95% CI, 0.09 to 0.55). Remifentanil-free regimen failed to decrease the pain score at 24 h (WMD: 0.10, 95% CI, -0.10 to 0.30) and 48 h (WMD: 0.15, 95% CI, -0.22 to 0.52) in comparison with remifentanil-based anaesthesia. After excluding trials with high heterogeneity, the dose of the remifentanil regimen was closely correlated with the postoperative pain score (P=0.03). In addition, the dose of the remifentanil regimen was not associated with the incidence of postoperative nausea and vomiting (PONV) (P=0.37).ConclusionsOur meta-analysis reveals that the low dose of remifentanil infusion is recommendable for general anaesthesia maintenance. No evidence suggests that remifentanil-free regimen has superiority in reducing postoperative pain. Moreover, remifentanil doesn’t have a dose dependent effect in initiating PONV.Trial registrationThe protocol of present study was registered with PROSPERO (CRD42022378360).

USING THE LEAN MANUFACTURING METHODOLOGY TO IMPROVE THE QUALITY OF THE ENTERPRISE'S BUSINESS PROCESSES

Formulation of the problem. In the conditions of global competition, lean production acquires special importance. For enterprises all over the world, the only way to survive in the market is to produce goods and provide services that most fully satisfy the demands of consumers, while using advanced management methods and technologies. Lean manufacturing is one of the modern tools of production organization and management technology, which is used to improve the quality of products and services, the most complete satisfaction of consumer needs, while ensuring a careful attitude to the company's resources and their savings.
 The purpose of the study is the further development of methodological, methodical and practical approaches to the concept of Lean manufacturing to improve the quality of the enterprise's business processes.
 Research methods. The research used scientific methods, such as theoretical generalization, comparison, analysis, synthesis.
 Result. In the process of research, a comparison of traditional business management and the concept of lean production was made and revealed. that the concept of lean production has such advantages as the transition from centralized, vertical management to horizontal, involvement of all employees in the process, teamwork, focus on rational use of resources. The concept of lean production is aimed at eliminating the following main types of losses: overproduction of goods; waiting for raw materials, semi-finished products, necessary service information; unnecessary movement of materials; redundant processing steps arising due to errors in the design of business processes; excessive stocks and business processes leading to their occurrence; unjustified rotation of personnel in the work process; production of waste; underutilization of employees' creative potential.
 Conclusions. Six main elements and 52 components (requirements) of Lean manufacturing are defined based on the SAE J4000 standard for more successful application of lean manufacturing ideas in practice production to analyze the levels of implementation of lean manufacturing methodology components and elements. The main elements of the concept of lean manufacturing, the development and improvement of which should be focused on, are: company management and trust in it; enterprise personnel; information environment; chain "supplier – organization – consumer"; product; business processes. An important role in the concept of lean manufacturing is played by the 5S ordering system and the system of general maintenance of TPM equipment .
 Keywords: lean manufacturing; losses; flow of value creation; 5S ordering system; system of general maintenance of TPM equipment; SAE standard J4000.

Lily LlHSFC2 coordinates with HSFAs to balance heat stress response and improve thermotolerance.

Heat stress transcription factors (HSFs) are core regulators of plant heat stress response. Much research has focused on class A and B HSFs, leaving those of class C relatively understudied. Here, we reported a lily (Lilium longiflorum) heat-inducible HSFC2 homology involved in thermotolerance. LlHSFC2 was located in the nucleus and cytoplasm and exhibited a repression ability by binding heat stress element. Overexpression of LlHSFC2 in Arabidopsis, tobacco (Nicotiana benthamiana), and lily, all increased the thermotolerance. Conversely, silencing of LlHSFC2 in lily reduced its thermotolerance. LlHSFC2 could interact with itself, or interact with LlHSFA1, LlHSFA2, LlHSFA3A, and LlHSFA3B of lily, AtHSFA1e and AtHSFA2 of Arabidopsis, and NbHSFA2 of tobacco. LlHSFC2 interacted with HSFAs to accelerate their transactivation ability and act as a transcriptional coactivator. Notably, compared with the separate LlHSFA3A overexpression, co-overexpression of LlHSFC2/LlHSFA3A further enhanced thermotolerance of transgenic plants. In addition, after suffering HS, the homologous interaction of LlHSFC2 was repressed, but its heterologous interaction with the heat-inducible HSFAs was promoted, enabling it to exert its co-activation effect for thermotolerance establishment and maintenance. Taken together, we identified that LlHSFC2 plays an active role in the general balance and maintenance of heat stress response by cooperating with HSFAs, and provided an important candidate for the enhanced thermotolerance breeding of crops and horticulture plants.