Agathis Research Articles

Phyllosphere of Agathis australis Leaves and the Impact of the Soil-Borne Pathogen Phytophthora agathidicida

Leaf surface microbial communities play an important role in forest ecosystems and are known to be affected by environmental and host conditions, including diseases impacting the host. Phytophthora agathidicida is a soil-borne pathogen that causes severe disease (kauri dieback) in one of New Zealand’s endemic trees, Agathis australis (kauri). This research characterised the microbial communities of the A. australis phyllosphere (i.e. leaf surface) using modern molecular techniques and explored the effects of P. agathidicida on those communities. Fresh leaves were collected from trees where P. agathidicida was and was not detected in the soil and characterisation of the leaf surface microbial community was carried out via high-throughput amplicon sequencing of the internal transcribed spacer (ITS) and 16S ribosomal RNA regions. Nutrients in leaf leachates were also measured to identify other possible drivers of microbial diversity. The dominant phyllosphere microbial phylum was Proteobacteria followed by Acidobacteria. The phyllosphere microbial richness of A. agathis associated with P. agathidicida-infected soils was found to be generally lower than where the pathogen was not detected for both prokaryote (bacterial) and fungal phyla. Leaf leachate pH as well as boron and silicon had significant associations with bacterial and fungal community structure. These findings contribute to the development of a comprehensive understanding of A. australis leaf surface microbial communities and the effects of the soil pathogen P. agathidicida on those communities.

Impacts of phosphite treatment on Phytophthora community assemblages and inoculum abundances in Phytophthora-infected forest soil

Phytophthora are causing declines in forest tree species worldwide and the chemical control treatment, phosphite, is the only treatment consistently shown to provide some protection to natural ecosystems from Phytophthora diseases. Phosphite inhibits Phytophthora growth and sporulation whilst boosting defence responses in the plant host. It is unclear, however, the extent of the impact of phosphite on Phytophthora species assemblages and inoculum abundances in soil around trees following treatment within natural ecosystems. In New Zealand, kauri (Agathis australis), an endemic and threatened foundation species, suffers from a dieback disease primarily caused by Phytophthora agathidicida. Phosphite is applied by trunk injection to kauri and has been shown to improve resin ‘bleed’ symptoms from basal trunk lesions and to promote recovery of thinned canopies. Phytophthora community and inoculum abundance were investigated in response to phosphite treatments at two field sites (Huia and Waitoki) in infected kauri stands in Auckland, New Zealand. At Huia, soil sampling and tree health surveying were conducted in November 2023 on trees treated with phosphite in 2012 as part of an earlier study. At Waitoki, the response to phosphite treatment was monitored 6 and 18 months following treatment. Phytophthora species were detected using soil baiting and metabarcoding of environmental DNA (eDNA) from soil and quantified with qPCR of root and soil DNA. Three species were detected with soil baiting (P. agathidicida, P. cinnamomi, and P. multivora) and two additional species with metabarcoding (P. pseudocryptogea, and an unknown clade 7 species similar to P. europaea). Phytophthora cinnamomi was the most abundant species, followed by P. agathidicida. Both species were more likely to occur together than by chance alone and were associated with declining tree health. The P. europaea-like species was in approximately 50% of the samples and was less likely to occur in roots with poorer health, or in association with P. agathidicida. The abundance of P. agathidicida inoculum was lower in the soil around the phosphite-treated trees than around the untreated control trees 1.5 years after treatment at Waitoki. Phosphite halted the lateral expansion of basal resin bleeds, and resin viscosity was reduced. Not only did phosphite treatments improve kauri dieback symptoms, but the phosphite treatments potentially had a direct impact on the epidemiology of the disease by reducing inoculum load around treated trees, with direct implications for disease management as an effective way to protect uninfected areas and minimise the spread of inoculum from infested zones.

The impacts of ecological disturbances on the diversity of biosynthetic gene clusters in kauri (Agathis australis) soil

BackgroundThe ancient kauri (Agathis australis) dominated forests of Aotearoa New Zealand are under threat from a multitude of ecological disturbances such as forest fragmentation, biodiversity loss, climate change, and the spread of the virulent soil pathogen Phytophthora agathidicida. Taking a wider ecosystem-level approach, our research aimed to explore the impacts of forest disturbance and disease outbreaks on the biosynthetic potential and taxonomic diversity of the kauri soil microbiome. We explored the diversity of secondary metabolite biosynthetic gene clusters (BGCs) in soils from a range of kauri forests that varied according to historical disturbance and dieback expression. To characterise the diversity of microbial BGCs, we targeted the non-ribosomal peptide synthetase (NRPS) and polyketide synthetase (PKS) gene regions for sequencing using long-read PacBio® HiFi sequencing. Furthermore, the soil bacterial and fungal communities of each forest were characterized using 16 S rRNA and ITS gene region sequencing.ResultsWe identified a diverse array of naturally occurring microbial BGCs in the kauri forest soils, which may offer promising targets for the exploration of secondary metabolites with anti-microbial activity against P. agathidicida. We detected differences in the number and diversity of microbial BGCs according to forest disturbance history. Notably, soils associated with the most undisturbed kauri forest had a higher number and diversity of microbial NRPS-type BGCs, which may serve as a potential indicator of natural levels of microbiome resistance to pathogen invasion.ConclusionsBy linking patterns in microbial biosynthetic diversity to forest disturbance history, this research highlights the need for us to consider the influence of ecological disturbances in potentially predisposing forests to disease by impacting the wider health of forest soil ecosystems. Furthermore, by identifying the range of microbial BGCs present at a naturally high abundance in kauri soils, this research contributes to the future discovery of natural microbial compounds that may potentially enhance the disease resilience of kauri forests. The methodological approaches used in this study highlight the value of moving beyond a taxonomic lens when examining the response of microbial communities to ecosystem disturbance and the need to develop more functional measures of microbial community resilience to invasive plant pathogens.

A new species of Mecodema Blanchard, 1853 (Coleoptera: Carabidae: Nothobroscina) restricted to the north-eastern arm of the Parataiko Range, Northland, Aotearoa – New Zealand

ABSTRACT In recent years the high plateaus and escarpments of the north-eastern area of the Parataiko Range, Northland, Aotearoa – New Zealand have been recognised by taxonomists as a biodiversity hotspot with several new plant species unique to the whenua (land) above the Waima Valley. The wet and cool climate limits the growth of kauri (Agathis australis) but has allowed for unique species (e.g. Coprosma waima, Olearia crebra) to adapt to the steep-sided slopes and damp soils of the plateaus. Northland is also recognised as a biodiversity hotspot for the ground beetle genus Mecodema, with 15 described species endemic to this region, almost double that of an equivalent North Island entomological region. We describe, M. haakuturi sp. nov., which is restricted to the north-eastern arm of the Parataiko Range and is one of only a few Mecodema species found above an altitude of 500 m in Northland. We provide an updated key of Mecodema species from the Northland region, including the northern Auckland/Northland boundary species M. rusticulus and the widespread coastal M. antarcticum. urn:lsid:zoobank.org:pub:CB67CF55-C486-4AFD-B0C1-5F7CAB86A419

The threat of a major tree pathogen to forest soil mesofauna food webs and ecosystem functioning

Tree pathogens threaten the survival of many forest foundation tree species worldwide. However, there is limited knowledge of how dieback of foundation tree species may threaten other components of forest ecosystems, such as soil biodiversity and associated ecosystem functions. Kauri (Agathis australis), threatened by the root-borne pathogen Phytophthora agathidicida, are culturally and ecologically significant tree species that exert great influence on soil properties. We aimed to characterise soil mesofauna community structure and energy fluxes in kauri forests and assess the potential threat that tree pathogens such as P. agathidicida pose to belowground ecosystems. We sampled soil mesofauna communities and identified specimens to functional feeding groups at 24 pairs of kauri and adjacent broadleaf trees in sites across the Waitākere Ranges Regional Park, Aotearoa – New Zealand. We attributed kauri canopy health scores, measured tree diameter, slope, forest floor depth, and soil carbon dioxide efflux. We also analysed soil samples for P. agathidicida presence, total carbon, and total nitrogen. We constructed soil mesofauna food webs associated with kauri and broadleaf trees, and assessed the uniqueness of food webs associated with kauri and the impacts of P. agathidicida on density, biomass, mean body mass, and energy fluxes of mesofauna taxonomic and trophic groups. We found omnivores with larger body mass at kauri where P. agathidicida was detected (i.e., P. agathidicida-positive soils). Compared to broadleaf trees, mesofauna density and biomass were lower in soils under kauri, and body masses of Symphyla and omnivores were smaller in soils under kauri. Differences in mesofauna community response variables between tree types were mainly modulated by the soil C:N ratio, which had positive effects under broadleaf and neutral to negative effects under kauri. Energy fluxes to detritivores and fungivores were greater under larger trees, regardless of tree type or P. agathidicida detection status. Our findings suggest that kauri support soil mesofauna food webs that are distinctly different from those found under broadleaf trees in the same habitat. A decreased presence of this foundation species may be linked to future impacts on soil mesofauna in this forest ecosystem with increasingly advanced stages of kauri dieback.

Orta Karadeniz Bölgesi (Türkiye) Agathidinae (Hymenoptera: Braconidae) altfamilyası üzerine faunistik katkılar

This study was conducted between 2003-2004 to investigate the Agathidinae (Hymenoptera: Braconidae) fauna of the Central Black Sea Region in Türkiye. For this purpose, Agathidinae samples were collected from different altitudes and habitats of Amasya, Çorum, Ordu, Samsun and Tokat provinces in the region. Seventeen species were determined and identified. These were twelve species in the genus Agathis, four species in the genera Bassus and one species in the genera Disophrys were recorded. Although all identified species were recorded before Türkiye, they are new records for the research area. The distributions and detailed locality records of the identified species are given.

Plant-pathogen management in a native forest ecosystem

Forest ecosystems all over the world are facing a growing threat from plant-disease outbreaks. As pollution, climate change, and global pathogen movement intensify, so too do the impacts of forest pathogens. In this essay, we examine a case study of the New Zealand kauri tree (Agathis australis) and its oomycetepathogen, Phytophthora agathidicida. We focus on the interactions between the host, pathogen, and environment - the building blocks of the 'disease triangle', a framework used by plant pathologists to understand and manage diseases. We delve into why this framework is more challenging to apply to trees than crops, taking into account the differences in reproductive time, level of domestication, and surrounding biodiversity between the host (a long-lived native tree species) and typical crop plants. We also address the difficulties in managing Phytophthora diseases compared to fungal or bacterial pathogens. Furthermore, we explore the complexities of the environmental aspect of the disease triangle. In forest ecosystems, the environment is particularly complex, encompassing diverse macro- and microbiotic influences, forest fragmentation, land use, and climate change. By exploring these complexities, we emphasize the importance of targeting multiple components of the disease triangle simultaneously to make effective management gains. Finally, we highlight the invaluable contribution of indigenous knowledge systems in bringing a holistic approach to managing forest pathogens in Aotearoa New Zealand and beyond.

Whole genome sequencing of Penicillium and Burkholderia strains antagonistic to the causal agent of kauri dieback disease (Phytophthora agathidicida) reveals biosynthetic gene clusters related to antimicrobial secondary metabolites.

Phytophthora agathidicida is a virulent soil pathogen of Aotearoa New Zealand's iconic kauri tree species (Agathis australis (D. Don) Lindl.) and the primary causal agent of kauri dieback disease. To date, only a few control options are available to treat infected kauri that are expressing symptoms of dieback disease. Previous research has identified strains of Penicillium and Burkholderia that inhibited the mycelial growth of P. agathidicida in vitro. However, the mechanisms of inhibition remain unknown. By performing whole genome sequencing, we screened the genomes of four Penicillium and five Burkholderia strains to identify secondary metabolite encoding biosynthetic gene clusters (SM-BGCs) that may be implicated in the production of antimicrobial compounds. We identified various types of SM-BGCs in the genome of each strain, including polyketide synthases (PKSs), non-ribosomal peptide synthetases (NRPSs), and terpenes. Across all four of the Penicillium strains, five SM-BGCs were detected that encoded the biosynthesis of napthopyrone, clavaric acid, pyranonigrin E, dimethyl coprogen and asperlactone. Across all five of the Burkholderia strains, three SM-BGCs were detected that encoded the biosynthesis of ornibactin, pyochelin and pyrrolnitin. Our analysis detected numerous SM-BGCs which could not be characterised. Further efforts should be made to identify the compounds encoded by these SM-BGCs so that we can explore their antimicrobial potential. The potential inhibitory effects of the compounds encoded by the SM-BGCs identified in this study may be worthy of further investigation for their effect on the growth and virulence of P. agathidicida.

Park Rangers and Science-Public Expertise: Science as Care in Biosecurity for Kauri Trees in Aotearoa/New Zealand

Park rangers hold a unique set of knowledge—of science, of publics, of institutional structures, of place, and of self—that should be recognised as valuable. For too long, models of the knowledge of scientists and publics have set people like rangers in an inbetweener position, seeing them as good at communicating, translating or negotiating from one side to the other, but not as making knowledge that is powerful in its own right. In this paper we argue that focus groups with park rangers across regional and conservation parks in Aotearoa/New Zealand reveal the complexity and multiplicity of rangers’ knowledge-making that shows the science-public model as incomplete. We argue further that the model is flawed for its focus on the making and holding of knowledge and not also on how it is enacted. Rangers, we argue, are responsible every day for making knowledgeable decisions for the health and well-being of parks and people. Making good decisions, though, requires not just knowledge but also care. An understanding of the many ways rangers know and enact their knowledge is important for biosecurity practice, especially in models of adaptive governance.

Chromosome-level assembly of the Phytophthora agathidicida genome reveals adaptation in effector gene families.

Phytophthora species are notorious plant pathogens, with some causing devastating tree diseases that threaten the survival of their host species. One such example is Phytophthora agathidicida, the causal agent of kauri dieback - a root and trunk rot disease that kills the ancient, iconic and culturally significant tree species, Agathis australis (New Zealand kauri). A deeper understanding of how Phytophthora pathogens infect their hosts and cause disease is critical for the development of effective treatments. Such an understanding can be gained by interrogating pathogen genomes for effector genes, which are involved in virulence or pathogenicity. Although genome sequencing has become more affordable, the complete assembly of Phytophthora genomes has been problematic, particularly for those with a high abundance of repetitive sequences. Therefore, effector genes located in repetitive regions could be truncated or missed in a fragmented genome assembly. Using a combination of long-read PacBio sequences, chromatin conformation capture (Hi-C) and Illumina short reads, we assembled the P. agathidicida genome into ten complete chromosomes, with a genome size of 57 Mb including 34% repeats. This is the first Phytophthora genome assembled to chromosome level and it reveals a high level of syntenic conservation with the complete genome of Peronospora effusa, the only other completely assembled genome sequence of an oomycete. All P. agathidicida chromosomes have clearly defined centromeres and contain candidate effector genes such as RXLRs and CRNs, but in different proportions, reflecting the presence of gene family clusters. Candidate effector genes are predominantly found in gene-poor, repeat-rich regions of the genome, and in some cases showed a high degree of duplication. Analysis of candidate RXLR effector genes that occur in multicopy gene families indicated half of them were not expressed in planta. Candidate CRN effector gene families showed evidence of transposon-mediated recombination leading to new combinations of protein domains, both within and between chromosomes. Further analysis of this complete genome assembly will help inform new methods of disease control against P. agathidicida and other Phytophthora species, ultimately helping decipher how Phytophthora pathogens have evolved to shape their effector repertoires and how they might adapt in the future.

Pro-Environmental Behaviour in Relation to Kauri Dieback: When Place Attachment Is Not Enough

The iconic kauri tree of Aotearoa New Zealand is under increasing threat due to the plant disease kauri dieback, with human activity believed to be a high-risk vector for spread of the fungus-like pathogen. Although forest hygiene measures have been introduced, concern exists over shortfalls in public compliance, particularly among those who live near or in infected areas. This qualitative study takes a community case-study approach to investigate the barriers and enablers of pro-environmental behavior in relation to kauri dieback. Twenty-one members of the public residing in a locale heavily impacted by kauri dieback were interviewed. Several issues that may lead to noncompliance with measures to protect kauri were identified, primarily centered around themes related to system knowledge, certainty and trust. Understanding community social dynamics, as well as cultivating and maintaining public trust in scientific and resource management authorities, is an important element for generating and improving pro-environmental behavior when uncertainty is high.

Prediction of sap flow with historical environmental factors based on deep learning technology

Sap flow is an important intermediate link that reflects the continuous soil–plant-atmosphere cycle. Therefore, it is important to predict the sap flow to analyze the amount of tree transpiration for assessing of water consumption. In this paper, we propose a new sap flow assessment using environmental factors based on a convolutional neural network-gated recurrent unit (CGRU) hybrid deep learning method. The model was trained and tested with the sap flow and environmental factors from 17,568 group observations from public SAPFLUXNET dataset. These group observations measured from January 1, 2012 to December 31, 2012, with acquisition interval of 30 min for one tree of New Zealand Agathis australis. After designed the CGRU structure by integrated a convolutional neural network (CNN) and a gated recurrent unit (GRU) neural network, the input variables were selected with a correlation analysis between sap flow and environmental factors. Additionally, the number of previous conditions were introduced into the input of the model. Results showed that when the number of previous conditions set to 16, the learning rate set to 0.01 with Adam optimization algorithm, the mean squared error, mean absolute percentage error, and coefficient of determination of the CGRU model were 0.00231, 22.31 and 0.948 respectively. Comparing results showed that the CGRU-based sap flow prediction model has more accuracy than other eight models participating in the test including the independent CNN, GRU, CNN-Long short-term memory (LSTM) and five traditional machine learning based models. The least time spent on training is also the CGRU model. The CGRU-based sap flow prediction model proposed in this paper can capture complex nonlinear dependencies and yield accurate assessments of sap flow. Our model we established in this paper can be useful for research on forest stand transpiration, water consumption and the soil–plant-atmosphere cycle.

Kāpia

Not long after I was absorbed in the latent energies in the Tsunami boulders and Len Lye’s kinetic systems, I returned to more-than-human geologies in my art practice. The catalyst for my recent video work Kāpia: fossils and remedies was a story of kāpia, a relic of an ancient forest commonly called Kauri gum by the settler-colonists, uncovered in a sand dune in the Hokianga harbour in the ‘far north’ of Aotearoa New Zealand. In long-ago climates, the ancestors of Kauri trees, Agathis australis, grew throughout the country and their traces can be found in the leaf fossil records and in amber, their resin, and the solidified pre-fossil resin, kāpia. Today, only a few stands of original Kauri forest remain in Te Tai Tokerau, Northland, and their future survival is uncertain. The gigantism of the Kauri tree evidences their deep prehistory when they dwelled with huge creatures on the continent of Gondwana in proto-Australia, the Pacific islands, India and Antarctica. Kauri are believed, controversially, to have survived the complete submergence of Aotearoa in the Miocene era, but now they must withstand a new pathogen. Phytophthora agathidicida, commonly called Kauri die-back, surfaced in the Anthropocene, just like COVID-19. We humans are asked by many iwi, tribes, to socially distance from these living ancestor-trees for their own survival, under conditions of rahui, or temporary prohibition. How might we protect bodies of trees, people and other more-than-human companions?

Is provenance or phylogeny a better predictor of growth and survival of a soil pathogen in leaf litter?

Exotic plants have the potential to increase pathogen inoculum that can affect native plants. New Zealand’s iconic kauri tree (Agathis australis) is threatened by disease caused by Phytophthora agathidicida, which is most prevalent in fragmented forests that have been invaded by or are adjacent to populations of exotic species. Exotic plants have been introduced intentionally (i.e., plantations and pastures) and unintentionally along the margins of kauri forests, yet it is unclear if invasive species play a role in pathogen spread. To determine the extent to which native and exotic plant litter supports P. agathidicida inoculum, we performed a phylogenetically controlled detached leaf assay. We inoculated 60 native and 44 invasive species’ leaves with three isolates of P. agathidicida collected from two different geographical regions of New Zealand, measured disease symptoms and re-isolated the pathogen from infected leaves. Lesions grew larger and faster on exotic leaves across all three isolates tested. However, pathogen recovery was not necessarily more likely from exotic leaves. In contrast, one of the three isolates grew faster when recovered from native compared with exotic leaves. Phylogeny did not predict disease expression. This data suggests that native and exotic plant litter may be reservoirs for P. agathidicida, but reservoir potential varies among isolates. These results also support key management tools used in New Zealand aimed at reducing pathogen spread by foot traffic in fragmented kauri forests, such as hygiene stations for shoe cleaning at trailheads and boardwalks in sensitive forest areas. Further, these tools may benefit forest management worldwide, as pathogens and exotic, invasive species increase at a global scale.

Sensitivity of the soil-borne pathogen Phytophthora agathidicida, the causal agent of kauri dieback, to the anti-oomycete fungicides ethaboxam, fluopicolide, mandipropamid, and oxathiapiprolin

The oomycete Phytophthora agathidicida is the causal agent of kauri dieback, which threatens the survival of endemic kauri (Agathis australis) forests in Aotearoa|New Zealand. Current chemical control of P. agathidicida involves the application of either a mixture of halogenated tertiary amines or phosphite salts with some success, but neither treatment cures the disease. Recently, four anti-oomycete fungicides, all with different modes of action, have become commercially available. Here, we determined the inhibition potential of these fungicides on three P. agathidicida isolates, using agar dilution assays. The average concentration required to inhibit mycelial growth by 50% (EC50) for ethaboxam, fluopicolide, and mandipropamid was 0.0916, 0.372, and 0.0196 µg/mL, respectively. Inhibition of P. agathidicida mycelia by oxathiapiprolin and its commercial formulation, Zorvec® Enicade®, was 0.000152 and 0.000309 µg/mL, respectively. Based on the EC50 values reported in this study, these fungicides are the most effective inhibitors of P. agathidicida mycelia when compared to previously screened fungicides, natural products, and plant extracts. Thus, their performance in this initial screening supports further research into their potential use as a kauri dieback management tool.

Implications of strontium isotope and trace element variability in New Zealand kauri (Agathis australis)

New Zealand kauri trees are an important dendrochonologic resource in the southern hemisphere due to their long lifespans and the correlation of ring widths to El Niño - Southern Oscillation (ENSO)-events. This study represents the first chronology-constrained dendrochemical investigation of New Zealand kauri. Strontium isotope (87Sr/86Sr) and trace element analyses are combined to determine the potential for provenance (spatial) and environmental (temporal) dendrochemical investigations utilizing the geochemistry of kauri wood. Soil Sr isotopic measurements match 80% of the corresponding analyses from local trees and two of the four study sites have kauri isotopic compositions tightly clustered and distinctive, supportive of provenance investigations. The two sites which record inter-tree isotopic variability, a combination of variables, including geologic, atmospheric (dust and sea spray), and anthropogenic (land-use), all likely influence the isotopic and trace element variations. Discrete annual events or fluctuating conditions are difficult to observe from the isotopic tree ring record, reflecting the potential mobility of Sr within tree rings. However, the preservation of long term geochemical trends suggest processes such as climate change or anthropogenic soil modification may still be preserved in the kauri record.

Advances and limitations in establishing a contiguous high-resolution atmospheric radiocarbon record derived from subfossil kauri tree rings for the interval 60–27 cal kyr BP

Radiocarbon dating is the most widely applied and reliable dating technique for providing chronological control during Marine Isotope Stage 3 (MIS3; ∼60–27 cal kyr BP). Past variations in the atmospheric concentration of radiocarbon mean a calibration curve is required. IntCal20 and SHCal20 calibration curves covering MIS3 are presently largely based on non-atmospheric records which, in combination with larger radiocarbon ( 14 C) dating uncertainties, results in significant smoothing and reduced resolution in calibration curve structure. Floating tree ring radiocarbon chronologies that are wiggle-matched to other palaeo records (particularly to Hulu Cave speleothems) have the potential to provide detailed structure to the MIS3 portion of extant calibration curves. New Zealand subfossil kauri ( Agathis australis ) trees are long-lived and are useful for constructing temporally-floating MIS3 atmospheric radiocarbon datasets. This paper presents extant and emerging data from several important Northland subfossil kauri locations (Omaha, Babylon Coast, Bream Bay, Kai Iwi Lakes, Mangawhai). We show the span of seven floating MIS3 kauri sequences (individual trees and chronologies) from which sequential radiocarbon series covering a total of 7556 years is now in development (representing 23% of the period 60–27 cal kyr BP). We also report radiocarbon dates for an additional 34 ancient kauri from MIS3 that can provide additional coverage. After these floating subfossil wood sequences have been produced, close to 40% of MIS3 (12,420 years) will be covered by contiguous subfossil kauri radiocarbon measurements. Based on our findings, we discuss the prospects and limitations for obtaining a highly resolved and precise atmospheric radiocarbon calibration curve comprehensively covering MIS3 using subfossil kauri.

Provenancing kauri: Reconstructing the supply of kauri timber into Auckland city, 1850–1889

AbstractThe 4500‐year kauri (Agathis australis) chronology includes ring width data from subfossil remains, 19th‐century building timbers and live kauri trees. It was assumed that the building timber data set represented a similar geographic spread to modern kauri sites but that assumption has not been tested empirically by provenancing kauri timber to the source region. To support provenancing, timber import patterns into Auckland between 1850 and 1889 CE were reconstructed, demonstrating that the archaeological assemblage is likely biased to west and east Auckland and the Coromandel Peninsula, particularly the east side. Improved understanding of kauri timber provenance informs future use of the data set for climate and environmental studies.

Assessing the effectiveness of oxathiapiprolin toward Phytophthora agathidicida, the causal agent of kauri dieback disease.

Phytophthora species cause disease and devastation of plants in ecological and horticultural settings worldwide. A recently identified species, Phytophthoraagathidicida, infects and ultimately kills the treasured kauri trees (Agathis australis) that are endemic to New Zealand. Currently, there are few options for managing kauri dieback disease. In this study, we sought to assess the efficacy of the oomycide oxathiapiprolin against several life cycle stages of two geographically distinct P. agathidicida isolates. The effective concentration to inhibit 50% of mycelial growth (EC50) was determined to be ∼0.1ng/ml, indicating that P. agathidicida mycelia are more sensitive to oxathiapiprolin than those from most other Phytophthora species that have been studied. Oxathiapiprolin was also highly effective at inhibiting the germination of zoospores (EC50 = 2-9ng/ml for the two isolates) and oospores (complete inhibition at 100ng/ml). In addition, oxathiapiprolin delayed the onset of detached kauri leaf infection in a dose-dependent manner. Collectively, the results presented here highlight the significant potential of oxathiapiprolin as a tool to aid in the control of kauri dieback disease.

Experimental induction of resins as a tool to understand variability in ambers

Abstract. Amber is chiefly known as a preservational medium of biological inclusions, but it is itself a chemofossil, comprised of fossilised plant resin. The chemistry of today's resins has been long investigated as a means of understanding the botanical sources of ambers. However, little is known about the chemical variability of resins and consequently about that of the ambers that are derived from particular resins. We undertook experimental resin production in Araucariacean plants to clarify how much natural resin variability is present in two species, Agathis australis and Wollemia nobilis, and whether different resin exudation stimuli types can be chemically identified and differentiated. The latter were tested on the plants, and the resin exudates were collected and investigated with Fourier-transform infrared attenuated total reflection (FTIR-ATR) spectroscopy to give an overview of their chemistry for comparisons, including multivariate analyses. The Araucariacean resins tested did not show distinct chemical signatures linked to a particular resin-inducing treatment. Nonetheless, we did detect two separate groupings of the treatments for Agathis, in which the branch removal treatment and mimicked insect-boring treatment-derived resin spectra were more different from the resin spectra derived from other treatments. This appears linked to the lower resin viscosities observed in the branch- and insect-treatment-derived resins. However the resins, no matter the treatment, could be distinguished from both species. The effect of genetic variation was also considered using the same stimuli on both the seed-grown A. australis derived from wild-collected populations and on clonally derived W. nobilis plants with natural minimal genetic diversity. The variability in the resin chemistries collected did reflect the genetic variability of the source plant. We suggest that this natural variability needs to be taken into account when testing resin and amber chemistries in the future.