16S rRNA Gene Research Articles

Detection of Theileria in cattle ticks (Rhipicephalus microplus) (Canestrini, 1888) in upper-northeastern Thailand

Theileria parasites were investigated from cattle ticks (Rhipicephalus microplus (Canestrini, 1888)) collected in 12 provinces in upper-northeastern Thailand based on the sequences of 18S rRNA and MPSP gene. Polymerase chain reactions (PCRs) and sequencing specific regions for the 18S rRNA gene revealed two species of Theileria pathogens; T. orientalis (n=42) and T. sinensis (n=31) with 94.50−100% identity. In the provinces of upper-northeastern Thailand, the nucleotide diversity of Theileria's 18S rRNA for T. sinensis and T. orientalis were 0% and 1.3%. respectively. The MPSP gene was used to categorize the T. orientalis genotypes. The sequences were compared with those available in the public database (GenBank) for species identification. Phylogenetic trees of Theileria were constructed from the MPSP gene sequences of our amplicons and those available in GenBank using maximum-likelihood and neighbor-joining analyses. The results revealed three identified genotypes: type 3, 5, and 7. Although the main carriers of T. orientalis are ticks in the genus Haemaphysalis, T. orientalis was the most frequently found in R. microplus in upper-northeastern Thailand. Theileria was frequent in Nong Khai, Mukdahan, and Loei, three Thai provinces that bordered the Lao PDR close to the Mekong River. Epidemiological surveys and control strategies in this region should be considered.

Tick-Borne Pathogens in Mongolian Ticks: The High Prevalence of Rickettsia raoultii and Its Public Health Implications.

In Mongolia, a substantial segment of the population is engaged in pastoralism, leading to frequent interactions with livestock and heightened exposure to tick-infested environments. Consequently, ticks and the endemic tick-borne pathogens (TBPs) they harbor present significant health threats. In May 2021, we conducted a study to evaluate the prevalence of 9 TBPs in ticks collected from four regions in Mongolia: Uvurkhangai, Tuv, Bayan-Ulgii, and Khentii. The pathogens surveyed included Anaplasma, Rickettsia, Bartonella, Borrelia, Ehrlichia, Babesia, Toxoplasma, Theileria, and lumpy skin disease virus. Molecular analysis of 1,142 ticks revealed that, although the majority of TBPs were not detected, 89.1% of the ticks were positive for Rickettsia. Genetic characterization using the 16S rRNA and gltA genes identified the pathogen identified the pathogen Rickettsia raoultii. Species identification indicated a predominance of Dermacentor nuttalli (70.0%) and Dermacentor silvarum (30.0%). These findings highlight the extensive prevalence of R. raoultii in Mongolia and underscore the urgent need for heightened awareness and preventive measures due to the high level of interaction between livestock and humans. The genetic similarity of R. raoultii sequences to those found in neighboring countries suggests potential cross-border transmission, underscoring the importance of conducting similar research in surrounding regions. This study advances our understanding of the epidemiology of tick-borne diseases in Mongolia and can inform public health strategies aimed at mitigating the risks associated with these infections.

New distribution record, morphological and molecular characterization of Dirofilaria (Nochtiella) tenuis (Nematoda: Onchocercidae) in raccoons (Procyon lotor) from the Yucatan Peninsula, Mexico.

Dirofilaria (Nochtiella) tenuis is a mosquito-borne subcutaneous parasite of raccoons, regarded as the causative agent of most human dirofilarial infections in North America. Despite the wide geographic range of raccoons in the Americas, the presence of this parasite has not been confirmed outside its known endemic areas in the Southern United States. Based on morphological and molecular data, we present the first record of D. (N.) tenuis in wild raccoons from the Yucatan Peninsula. Adult thread-like worms recovered from subcutaneous tissues of wild raccoons were analyzed with light microscopy, histology, scanning electron microscopy (SEM), and 18S and 28S rRNA gene sequencing for identification and phylogenetic analysis. The collected nematodes were identified as D. (N.) tenuis based on their morphology. SEM analysis revealed details about different facial ornamentations in male worms, which had not been previously described. Molecular and phylogenetic analyses confirmed morphological observations by placing our specimens within clades of the Dirofilaria genus. Our findings represent the first molecular characterization for this nematode and extend the geographical range of this parasite to Mexico. Further studies are required for a more accurate picture of the epidemiology of this filarioid across Mexico and other areas overlapping the raccoon's range.

Root inoculation with soil-borne microorganisms alters gut bacterial communities and performance of the leaf-chewer Spodoptera exigua.

Soil-borne microorganisms can impact leaf-chewing insect fitness by modifying plant nutrition and defence. Whether the altered insect performance is linked to changes in microbial partners of caterpillars remains unclear. We investigated the effects of root inoculation with soil bacteria or fungi on the gut bacterial community and biomass of the folivore Spodoptera exigua. We also explored the potential correlation between both parameters. We performed herbivory bioassay using leaves of tomato plants (Solanum lycopersicum), measured caterpillar weight gain and characterized the gut bacterial communities via 16S rRNA gene metabarcoding. All soil microbes modified the gut bacterial communities, but the extent of these changes depended on the inoculated species. Rhizophagus irregularis and Bacillus amyloliquefaciens had opposite effects on S. exigua weight. While plant inoculation with the fungus influenced gut bacterial diversity, B. amyloliquefaciens also affected the community composition. A reduced abundance of two S. exigua enterococcal symbionts correlated with decreased insect biomass. Our results show that soil microorganisms can induce plant-mediated changes in the gut bacterial community of foliar-feeding caterpillars. We propose that the impact of these alterations on insect performance might rely on specific adaptations within the gut bacteria, rather than solely on the occurrence of changes.

Water-insoluble dietary fiber from walnut relieves constipation through Limosilactobacillus reuteri-mediated serotonergic synapse and neuroactive ligand-receptor pathways.

Dietary fiber can alleviate functional constipation (FC) by modulating the gut microbiota. To clarify the prebiotic properties of walnut insoluble dietary fiber (WIDF), we explored its structural characteristics and laxative mechanism. A galacturonic acid and glucose-rich WIDF was isolated from walnuts by using a complex enzymatic method. Animal experiments results showed that WIDF could effectively alleviate the symptoms of loperamide-induced FC in mice, including shortening the defecation time, increasing the wet weight and water content of feces, and promoting intestinal motility. WIDF might alleviate FC through activating serotonergic synapse and inhibiting the delta-opioid receptor/inducible nitric oxide synthase (Oprd/iNOS) pathways. Importantly, WIDF treatment altered the structure and composition of the gut microbiota. Correlation analysis revealed that Bacillus and its dominant ASV17, which is considered to be the key microbe for constipation alleviation, were strongly associated with constipation phenotypes. Based on pure culture and 16S rRNA gene phylogenetic analysis, Limosilactobacillus reuteri (L. reuteri), which is 100 % similar to ASV17, was isolated and identified from the feces of WIDF-treated mice. L. reuteri relieved FC by modulating serotonergic synapse and the Oprd/iNOS pathways. These results suggested that WIDF and L. reuteri treatment is a prospective strategy for the prevention of constipation.

Bioconversion of sulfamethazine-contaminated chicken manure by black soldier fly larvae: Effects on antibiotic resistance genes and microbial communities.

Sulfamethazine (SM2), a widely detected antibiotic in livestock manure, poses environmental and health risks due to its persistence and the proliferation of antibiotic resistance genes (ARGs). In this study, we investigated the degradation of SM2 and the elimination of sulfonamide ARGs (sul1 and sul2) in chicken manure contaminated with varying concentration of SM2 by black soldier fly larvae (BSFL). Quantitative PCR and 16S rRNA gene sequencing were employed to monitor changes in sulfa ARGs and microbial community composition within both the larvae gut and chicken manure. During the 12-day test period, BSFL exhibited strong tolerance to SM2, significantly reducing SM2 concentrations by 80.54%-92.22% across different treatment groups. Concurrently, the abundance of sul1 and sul2 decreased by 79.27% and 79.92% in chicken manure, respectively. Additionally, microbial genera such as Firmicutes (47.18-65%) and Bacillus (9.32-10.25%), which were enriched in both the BSFL gut and chicken manure, were identified as potential contributors to SM2 degradation. These findings provide a promising biotechnological strategy for mitigating antibiotic contamination in livestock manure.

Molecular patterns of microbial and metabolic interactions in septic patients with persistent lymphopenia

BackgroundPersistent lymphopenia can be regarded as an important index of acquired immune dysfunction in sepsis. Whether the specific immune factor changes in septic patients with lymphopenia and the correlation to gut microbiota and metabolites remain unclear. MethodsThis single-center prospective observation conducted lymphocyte subgroup analysis of blood samples and 16S rRNA gene amplicons sequencing and untargeted metabolomics analysis of fecal samples from 36 subjects with the persistent (≥3d) (n=21) and non-persistent lymphopenia (<3d) (n=15). ResultsThe persistent lymphopenia showed higher the 28d mortality and 90d mortality, while significantly lower CD3+T/LY, CD3+T cells, CD3+CD4+T cells, CD3+CD8+T cells, Th1 cells, Th2 cells, CD45RA+Treg cells. The 16S rRNA results showed that Staphylococcus, Peptostreptococcus, Bulleidia, Leuconostoc were significant enriched in the persistent lymphopenia. The metabolomics analysis showed that α-Ketoisovaleric acid was increased and 7-DHCA, α-MCA, β-MCA, HCA, LCA-3S, CA, UCA and Citramalic acid were decreased in the persistent lymphopenia. ConclusionIn the process of interaction between host receptors and gut microbiota in patients with persistent lymphopenia sepsis, with a significant reduction in gut microbiota diversity and bile acid metabolites. That can affect various inflammatory pathways of gut immune cells, causing immune dysfunction in the body, which may be one of the main causes of death.

Dynamic change of gut microbiota in head and neck concurrent chemoradiotherapy patients and its potential value in the prediction of acute oral mucositis grade as well as quality of life.

Radiotherapy is the major therapy for head and neck squamous cell carcinoma (HNSCC). However, whether gut microbiota changes in HNSCC patients who received concurrent chemoradiotherapy remains unclear. This study aimed to investigate the dynamic change of gut microbiota composition, construct the first radiotherapy-related gut microbiota database in these patients and identify the potential value of the gut microbiota changing in the prediction of acute oral mucositis grade as well as patients' life quality. We enrolled 47 HNSCC patients who scheduled with concurrent chemoradiotherapy. The field was irradiated with a total dose of 66-70Gy in 33-35 fractions. All the patients received 2-3 cycles of platinum-based chemotherapy. After feces specimens collected, bacterial genomic DNA was isolated using magnetic beads and then analyzed by the Illumina MiSeq Sequencing System based on the V3-V4 hypervariable regions of the 16S rRNA gene. 194 genera which belonged to 27 phyla were found in 141 samples. Increased abundance of microbiota in diversity and richness was observed in mid-radiotherapy group. Bacteroides, Blautia, Phascolarctobacterium were three main genera in all three groups and the mid-radiotherapy group had the highest relative abundance of Phascolarctobacterium. What is more, most significantly altered bacteria shared the same variation pattern which was increased in mid-radiotherapy while decreased to the almost same level of as pre-radiotherapy in post-radiotherapy group. Further analysis indicated that Bacteroidetes showing an upward trend while Proteobacteria declining in higher grade of acute mucositis. Moreover, relatively low abundant Proteobacteria was significantly correlated with high-grade acute oral mucositis. As for the quality of life, Lactobacillales and Actinomycetales were specifically found in better life quality group. However, Clostridia_UCG_014, Eubacteriaceae, UCG_010 and Moraxellaceae were unique abundantly present in worse life quality group. Chemoradiotherapy can affect the composition of the gut microbiota in HNSCC patients during the mid-term of treatment. Yet self-stabilized ability maintained the gut microbiota homeostasis. Dynamic change of specific species could help predict acute oral mucositis grade and characterize different quality of life group in these patients.

Influence of hormonal factors, number of sexual partners, surgical intervention on gastrointestinal and urogenital microbiota of patients endometriosis

Endometriosis is associated with gastrointestinal (GI) and urogenital (UG) microbial dysbiosis in patients with endometriosis (P-EOSIS). Sexual partner exposure may contribute to microbial dysbiosis but has not been studied in P-EOSIS. We hypothesized that sexual partner number, hormonal and surgical therapy would affect GI/UG microbial dysbiosis in P-EOSIS. Urine, fecal and vaginal swabs from control (n = 15) and P-EOSIS (n = 33) were collected on the day of surgery (DOS) and ∼1-3 weeks post-surgical intervention (PSI). Control and P-EOSIS were grouped based on hormonal therapy (HT) to determine the effect of HT on microbial profiles, Control (HT n = 8; no HT n = 7) and P-EOSIS (HT n = 18; no HT n = 15). Samples underwent DNA extraction and sequencing of the V4 region of 16S rRNA gene. Sequences were processed using QIIME2 and amplicon sequence variants (ASV) were analyzed for microbial differences. Pearson's and Spearman correlation analyses determined associations among microbial features and sexual partner exposure. P-EOSIS had microbial dysbiosis characterized by unique GI/UG bacteria and altered microbial richness and diversity. Hormonal and surgical intervention in P-EOSIS restored GI microbial diversity. Increased sexual partner exposure decreased GI/UG microbial diversity. P-EOSIS who had 10 or more sexual partners had greater microbial dysbiosis compared to 4-6 partners. Surgical intervention negatively correlated with sexual partner numbers and GI/UG microbial abundance. Increased sexual partner exposure may enhance microbial dysbiosis in P-EOSIS and diminish the effectiveness of HT and surgical interventions.

Evaluating the role of recalcitrant dissolved organic matter in bacterial community dynamics in urbanized freshwater ecosystems

Dissolved organic matter (DOM) and recalcitrant dissolved organic matter (RDOM) play distinct roles in shaping microbial communities. However, characterizing these roles is difficult, especially in ecosystems subjected to varying degrees of anthropogenic influence. This study investigated the molecular compositions and ecological impacts of DOM and RDOM in the Fen River, Shanxi Taiyuan, comparing pristine upstream regions with highly urbanized downstream areas. Using 16S rRNA gene sequencing and LC-MS-based metabolomics, we observed significant shifts in microbial community composition, diversity, and metabolic functions. Upstream communities, characterized by higher diversity, were dominated by Bacteroidota, Proteobacteria, and Cyanobacteria, while downstream communities, influenced by pollution, exhibited increased expression of genes related to amino acid metabolism. Fluorescence spectroscopy and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) revealed that upstream DOM contained higher proportions of complex, high molecular weight compounds, including significant proportions of carboxyl-rich alicyclic molecules (CRAM) and island of stability (IOS) compounds, which play key roles in long-term carbon storage and microbial carbon sequestration. In contrast, downstream DOM was characterized as having lower aromaticity and more saturated compounds, with reduced proportions of CRAM and IOS, reflecting the impact of anthropogenic activities. These findings underscored the critical roles of CRAM and IOS in regulating DOM stability and microbial communities, further highlighting the need for targeted pollution control strategies to preserve ecosystem function in urbanized water bodies.

Identification of cadmium-tolerant plant growth-promoting rhizobacteria and characterization of its Cd-biosorption and strengthening effect on phytoremediation: Development of a new amphibious-biocleaner for Cd-contaminated site

The use of plant growth-promoting rhizobacteria (PGPR) in decontaminating cadmium-contaminated soil and water is a sustainable and eco-friendly approach. This study aimed to isolate a PGPR strain from the rhizosphere soil of Solanum nigrum and evaluate its potential and mechanisms in remediating Cd-contaminated environments. The results showed that the isolated strain, Klebsiella sp. AW2, can tolerate 240 mg/L Cd2+. Batch biosorption experiments indicated that the optimal conditions for PGPR biosorption were a pH of 5.0, a biosorbent dosage of 1.0 g/L, and a Cd2+ concentration of 10 mg/L, resulting in a biosorption rate of 40.99%. Model fitting results revealed that the Cd biosorption process followed a uniform surface monolayer chemisorption mechanism, likely involving complexation with functional groups such as -NH, -OH, and -C=O, according to Fourier transform infrared spectrometer and desorption experiments. Furthermore, pot experiments demonstrated that PGPR application significantly enhanced the phytoremediation efficiency of Cd-contaminated soil, increasing the phytoextraction ratio by 32.41%. This improvement was primarily achieved by promoting S. nigrum growth and facilitating Cd horizontal transfer from rhizosphere soil to plants through influencing the rhizosphere soil physicochemical properties and Cd2+ influx in roots. In addition, the copy number of the 16S rRNA gene of the PGPR revealed that the PGPR was predominantly localized in the rhizosphere soil, directly leading to increased availability of Cd for plant uptake. Overall, these findings indicate that Klebsiella sp. AW2 is a promising biocleaner for Cd-contaminated environments and provide valuable insights into the application of biosorbents in phytoremediation efforts.

A putative new Besnoitia species in the southern black-eared opossum Didelphis aurita

Besnoitia spp. are cyst-forming coccidian parasites with a broad host range, infecting various wild and domestic animal species. Northamerican opossums (Didelphis virginiana) are severely affected by the infection with B. darlingi. This study presents a case of infection with Besnoitia in a road-killed female southern black-eared opossum (Didelphis aurita) in Misiones, Argentina. Many 0.5 to 1 mm cysts were observed in several muscles and visceral organs and were microscopically identified in skeletal muscles, tongue, and heart. Histological analysis disclosed multiple spherical cysts with a myriad of bradyzoites like-cells and a well-defined cyst wall. A small number of degenerate and ruptured cysts, surrounded by mild to moderate inflammation were observed. Genomic DNA from an individual cyst and muscle was extracted and ITS1 marker and 18S rRNA gene fragments from sarcocystid protozoa were successfully amplified by PCR and sequenced. The 18S sequence exhibited 100% identity with sequences of B. darlingi and B. oryctofelisi. Comparison of the complete ITS1 sequence (259 bp) revealed an identity of 99.2% with B. oryctofelisi and 97.7% with B. darlingi. This result together with the phylogeny positioning, suggest that the Besnoitia sp. in the present case differ from B. darlingi, being closely related with B. oryctofelisi.

Investigating the genomic and metabolic abilities of PGPR Pseudomonas fluorescens in promoting plant growth and fire blight management.

Pseudomonas fluorescens is commonly found in diverse environments and is well known for its metabolic and antagonistic properties. Despite its remarkable attributes, its potential role in promoting plant growth remains unexplored. This study examines these traits across 14 strains residing in diverse rhizosphere environments through pangenome and comparative genome analysis, alongside molecular docking studies against Erwinia amylovora to combat fire blight. Whole genome analysis revealed circular chromosome (6.01-7.07Mb) with GC content averaging 59.95-63.39%. Predicted genes included 16S rRNA and protein-coding genes ranging from 4435 to 6393bp and 1527 to 1541bp, respectively. Pangenome analysis unveiled an open pangenome, shedding light on genetic factors influencing plant growth promotion and biocontrol, including nitrogen fixation, phosphorus solubilization, siderophore production, stress tolerance, flagella biosynthesis, and induced systemic resistance. Furthermore, pyrrolnitrin, phenazine-1-carboxylic acid, pyoluteorin, lokisin, 2,4-diacetylpholoroglucinol and pseudomonic acid were identified. Molecular docking against key proteins of E. amylovora highlighted the high binding affinities of 2,4-diacetylphloroglucinol, pseudomonic acid, and lokisin. These findings underscore the multifaceted role of P. fluorescens in plant growth promotion and biocontrol, with key biomolecules showing promising applications in plant growth and defense against pathogens.

First Detection of Vibrio parahaemolyticus in Migratory Birds in Egypt: Antibiogram, Virulence, and Resistance Gene Profiles Indicating Zoonotic and Public Health Risks.

This study examined the occurrence of Vibrio parahaemolyticus obtained from migrating birds, marking the first instance of such research conducted in Egypt. The study assessed potential risks using an antibiogram, virulence characteristics, antibiotic-resistance, and gene profile. Randomly collected 80 samples were tested for V. parahaemolyticus. Eleven (13.75%) samples were V. parahaemolyticus-contaminated. All isolates were positive for 16SrRNA and species-specific toxR genes. Interestingly, our strain is genetically similar to human and shrimp isolates, suggesting zoonotic transmission may pose a health danger. All isolates had 100% l-lysine decarboxylase, 45.45% beta-hemolytic, and 100% l-ornithine decarboxylase activity. All isolates displayednol-arginine decarboxylase activity. Notably, every isolate possessed a minimum of two virulence genes. In addition, the profiles of virulence genes were identified, tdh + /trh + (27.3%), tdh-/trh + (27.3%), and tdh + /trh- (45.4%). Out of the V. parahaemolyticus isolates, 18.2% (2/11) were extensively drug-resistant (XDR) to six different antimicrobials classes and possessed the blaTEM, blaOXA, sul1, and tetA genes. Furthermore, 63.6% of the isolates displayed multidrug resistance (MDR). The correlation highlights a strong relationship between phenotypic and genotypic resistance. Besides thestrong correlation between virulence and resistance genes.In summary, this work highlighted the presence of newly identified MDR and XDR V. parahaemolyticus carried toxR, trh, and tdh virulence genes, as well as blaTEM, blaOXA, sul1, and tetA resistance genes in migratory birds, indicating a significant public health risk.

Facivitalis istanbulensis gen. nov., sp. nov., a novel member of the family Sphingomonadaceae with the potential for aromatic-degradation isolated from Jet A1 fuel.

A novel gram-stain-indeterminate, rod-shaped, endospore-forming, motile, aerobic bacterium, designated JETA1-E2T, was isolated from aircraft fuel Jet A1 sample. The strain showed high pairwise similarity values of partial 16S rRNA gene sequences to Sphingomonas paucimobilis (MT367853) (99.42%), Sphingomonas sanguinis (MF319771) (99.34%), and Sphingomonas pseudosanguinis (HE716953) (99.27%) within the family Sphingomonadaceae. However, API test results revealed that the strain JETA1-E2T differed from these type strains. The phylogenetic tree based on the whole genome and the phylogenomic tree generated with the UBCG tool showed that the strain JETA1-E2T formed a distinct monophyletic clade within the family Sphingomonadaceae, and clustered distantly with the genera Sphingomonas and Sphingobium. The predominant respiratory quinone is Q-10. The major fatty acids are C16:0 and summed feature 8 (C18:1ω7c and/or C18:1ω6c). C19:0 is present in small amounts. The polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, aminophospholipid, unidentified glycolipid, and two unidentified sphingoglycolipids. The only polyamine is putrescine in minor amounts. The DNA G + C content of the type strain is 66.5mol%. Several unique genes in the strain JETA1-E2T may contribute to fight against various stressors, virulence and pathogenicity, as well as survival in challenging conditions. The strain JETA1-E2T contains 100 of the characterised proteins available in the HADEG database of which 58% of these are involved in metabolic process of aromatics degradation. The findings indicate that the strain JETA1-E2T has the potential to metabolise hydrocarbons such as fuel, especially aromatic compounds. Based on the results of polyphasic taxonomic analyses, the strain JETA1-E2T represents a novel species in a new genus in the family Sphingomonadaceae for which the name Facivitalis istanbulensis gen. nov., sp. nov. is proposed. The type strain of Facivitalis istanbulensis is JETA1-E2T (DSM 117971T = LMG 33634T = KUEN 1206 (B) F3-1-1T).

Altererythrobacter arenosus sp. nov., Isolated from Marine Sediment.

Strain CAU 1644T was isolated from sea sand at Ganghwa Island, Republic of Korea. The bacterium is aerobic, Gram-staining negative, yellow-colored, non-motile, catalase-positive, and rod-shaped. Cells of strain CAU 1644T grew at 20-40°C and pH 6.0-10.0 with 0%-3.0% (w/v) NaCl. The 16S rRNA gene sequences of Altererythrobacter epoxidivorans CGMCC 1.7731T (identity, 98.5%) and A. xiamenensis CGMCC 1.12494T (98.0%) had the highest similarities to strain CAU 1644T. Strain CAU 1644T exhibits affiliation with the genus Altererythrobacter by phylogenetic trees based on the 16S rRNA gene and core-genome sequences. The predominant quinone was ubiquinone-10 (Q-10) and the major fatty acids were summed feature 8 (C18:1ω7c and/or C18:1ω6c) and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain CAU 1644T were 77.6% and 20.1% with A. epoxidivorans CGMCC 1.7731T, respectively, and 77.4% and 21.0% with A. xiamenensis CGMCC 1.12494T. The G + C content is 62.5%. Based on phenotypic and genotypic analyses, strain CAU 1644T represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter arenosus sp. nov., is proposed. The type strain is CAU 1644T (= KCTC 92082T = MCCC 1K07086T).

The impact of fluconazole use on the fungal and bacterial microbiomes in recurrent Vulvovaginal Candidiasis (RVVC): a pilot study of vaginal and gastrointestinal site interplay.

Recurrent Vulvovaginal Candidiasis (RVVC) is a problematic clinical condition for which fluconazole treatment is commonly prescribed. This study investigated the interkingdom vaginal and gastrointestinal microbiomes of RVVC patients who use fluconazole intermittently or as longer-term maintenance therapy for symptom management and compared them to healthy controls. Vaginal swabs and fecal samples were collected. A novel interkingdom analysis was performed using 16S rRNA and ITS1 gene sequencing to compare the diversity and taxonomic composition of vaginal microbiome (VMB) and gastrointestinal microbiome (GIMB). Twenty-seven women participated: 10 intermittent users and healthy controls and 7 maintenance therapy. The study revealed that microbiomes of fluconazole users do not differ in diversity metrics from healthy controls. RVVC patients using intermittent fluconazole displayed a higher abundance of vaginal C. albicans than healthy controls. Candida species pairings were not commonly observed between sites in individuals and, as such a fecal reservoir is unlikely to be implicated in recurrent symptomatology. In many of the RVVC non-Candida fungal spp. were identified in the vaginal microbiome. Users of fluconazole displayed elevations of the CST-I (Community State Type 1) associated bacterium L. crispatus. All participants displaying vaginal Candida spp. belonged to either bacterial CST-I or CST-III (Community State Type 3- L. iners associated). To our knowledge, this is the first study to compare the interkingdom VMB-GIMB of women with RVVC using oral fluconazole. As fluconazole users in this study represent a typical RVVC population, trends observed in microbial abundance require further analysis to establish fluconazole's long-term microbiome safety. Examining the microbiome at both sites adds to the current understanding of microbial associated with the condition.

Regionalization of intestinal microbiota and metabolites in the small intestine of the Bactrian camel

IntroductionPeyer's patches (PPs) are crucial antigen-inductive sites of intestinal mucosal immunity. Prior research indicated that, in contrast to other ruminants, PPs in the small intestine of Bactrian camels are found in the duodenum, jejunum, and ileum and display polymorphism. Using this information, we analyzed the microbial and metabolic characteristics in various segments of the Bactrian camel's small intestine to further elucidate how the immune system varies across different regions. MethodsIn this study, the microbiota and metabolite of 36 intestinal mucosal samples, including duodenal (D-PPs), jejunal (J-PPs), and ileal PPs (I-PPs), were profiled for six Bactrian camels using 16S rRNA gene sequencing and liquid chromatography with tandem mass spectrometry (LC-MS/MS). To confirm meaningful associations, we conducted connection analyses on the significantly different objects identified in each group's results. ELISA was used to analyze the levels of IgA, IgG, and IgM in the same tissues. ResultsThe microbiota and metabolite profiles of J-PPs and I-PPs were found to be similar, whereas those of D-PPs were more distinct. In J-PPs and I-PPs, the dominant bacterial genera included Clostridium, Turicibacter, and Shigella. In contrast, D-PPs had a significant increase in the abundance of Prevotella, Fibrobacter, and Succinobacter. Regarding the metabolomics, D-PPs exhibited high levels of polypeptides, acetylcholine, and histamine. On the other hand, J-PPs and I-PPs were characterized by an enrichment of free amino acids, such as L-arginine, L-glutamic acid, and L-serine. These metabolic differences mainly involve amino acid production and metabolic processes. Furthermore, the distribution of intestinal immunoglobulins highlighted the specificity of D-PPs. Our results indicated that proinflammatory microbes and metabolites were significantly enriched in D-PPs. In contrast, J-PPs and I-PPs contained substances that more effectively enhance immune responses, as evidenced by the differential distribution of IgA, IgG, and IgM.DiscussionThe intestinal microenvironment of Bactrian camels displays distinct regional disparities, which we propose are associated with variations in immunological function throughout different segments of the small intestine. This study highlights the specific traits of the intestinal microbiota and metabolites in Bactrian camels, offering a valuable reference for understanding the relationship between regional intestinal immunity and the general health and disease of the host.

Long-Read Sequencing Revealing the Effectiveness of Captive Breeding Strategy for Improving the Gut Microbiota of Spotted Seal (Phoca largha).

The spotted seal (Phoca largha) is the sole pinniped species that can reproduce in China and has been classified as the First-Grade State Protection animal. The conventional method for the protection and maintenance of the spotted seal population is the captive maintenance of the species in artificially controlled environments. Nevertheless, the efficacy of the captive strategy remains uncertain, with the potential to impact the health of spotted seals through alterations in gut microbiota. In this study, PacBio sequencing based on the full-length of the bacterial 16S rRNA gene was applied to faeces from captive and wild spotted seals, thereby providing a first reference for the gut microbiota profile of spotted seals at the species scale. The gut microbiota of captive spotted seals was found to be more diverse than that of the wild population. The gut microbiota of spotted seals exhibited notable variation due to captive breeding, with an enrichment of Firmicutes and a reduction in Proteobacteria. The results of the co-occurrence network analysis indicated that the gut microbiota of captive spotted seals exhibited a greater degree of complexity and stability in comparison to that observed in their wild counterparts. The analysis of community assembly mechanisms revealed an increased determinism for the gut microbiota of captive individuals, with a concomitant decrease in the contribution of drift. Furthermore, the results of the predicted functions indicated a reduction in stress responses and an enhanced ability to metabolise sugars in the gut microbiota of captive spotted seals. In conclusion, the results of this study provide evidence that the current captive breeding strategy is an effective approach for improving the gut microbiota of spotted seals. Furthermore, this study demonstrates the potential of monitoring the gut microbiota to assess the health of marine mammals and inform conservation strategies for endangered species.

Imaging of Gut Bacterial Macroscopic Changes in Simulated Microgravity-Exposed Rats via In Vivo Metabolic Labeling.

The impact of the microgravity environment on gut bacteria has been widely recognized to induce notable gastrointestinal pathology during extended spaceflight. However, most current studies for gut microbiome homeostasis profiling are based on the 16S rRNA gene sequencing of fecal samples; this technology faces challenges in analyzing gut bacterial alterations in situ, dynamically, and with high spatiotemporal resolution. Herein, we present the utilization of bioorthogonal metabolic labeling for noninvasive imaging of gut bacterial macroscopic changes in simulated microgravity (SMG) rats. After being subsequently labeled with the metabolic reporters d-Ala-N3 and ICG-DBCO through click chemistry, it was shown that SMG can trigger obvious perturbation of gut bacteria, evidenced by the significant increase in the total bacterial content and spatial distribution variations. Such a difference was accompanied by the occurrence of intestinal inflammation and tissue damage. Compared with 16S rRNA genome analysis focusing on composition and diversity, the metabolic labeling strategy provides unprecedented insights into the macroscopic changes of the gut bacterial content and distribution under SMG. Our study will be helpful for investigating the biological implication of SMG-induced imbalance in gut bacteria, potentially promoting the deep investigation of the complex gastrointestinal pathology in space biomedicine.