Background: This study explores how structured training program enhances healthcare professionals’ awareness of lifestyle modifications for hypertension. Assessing its impact on both knowledge and patient guidance, with an aim to improve healthcare delivery by equipping professionals with informed strategies for lifestyle modification. Design and Methods: A quasi-experimental design was employed with sample size of 750 HCPs. The Hypertension Knowledge-Level Scale (HK-LS), a modified questionnaire based on guidelines from the World Health Organization (WHO), was utilized to assess participants’ understanding of hypertension, knowledge of lifestyle modification, and clinical practice. A 12-week online course comprising seven modules was delivered to the HCPs, followed by pre- and post-tests to measure changes in knowledge regarding lifestyle modifications. Results: The analysis revealed a substantial increase in HCPs knowledge levels post-intervention compared to pre-intervention regarding hypertension (t = 4.90, p = 0.001), lifestyle modification (t = 3.62, p = 0.001), and their proficiency in offering lifestyle advice to patients (t = 3.56, p = 0.001). In addition to these positive outcomes among HCPs, the intervention also highlighted significant gender disparities in attendance and outcomes among participants. Females exhibited a higher attendance rate of 75% compared to males, with consequent improvements in practice, while males showed a more substantial enhancement in knowledge levels post-intervention. These findings underscore the importance of addressing gender-specific barriers to improvement, such as socio-cultural factors for females and preconceived notions for males, to optimize the effectiveness of future interventions in promoting healthier behaviors and outcomes. Conclusions: Structured training programs are imperative for enhancing HCPs’ knowledge of lifestyle modification, particularly in the context of hypertension, as a crucial component of primary prevention strategies. This signifies the effectiveness of the structured training program in enhancing HCPs’ comprehension of hypertension management and their ability to guide patients toward adopting healthier lifestyles.