GC-MS Analysis Of Leaf Extract Research Articles

In vitro Antioxidant and Antibacterial Activities of Jatropha heynei Leaf Extract and GC-MS Profiling

Aim: Jatropha heynei N. P. Balakr. of family Euphorbiaceae is an endemic herb with ethno medicinal values has not been studied for phytochemical analysis. The present study investigates the antibacterial, antioxidant activity and GC-MS profiling of J. heynei leaf extract. Materials and Methods: The cyclic voltammetry technique was employed to study the antioxidant activity of leaf extract. In vitro antibacterial activity was done by agar well diffusion assay. Further, Chemical profiling of methanolic leaf extract of J. heynei was done by the GC-MS analysis. Results: Cyclic voltammograms of extract revealed two anodic peaks at 0.12 and 0.22V when anodic current was applied at scan rate of 50 mV s-1. This low oxidation peak potential in the extract suggested its good antioxidant property. In vitro antibacterial activity extract showed good inhibitory effect against Staphylococcus aureus with zone of inhibition 14±0.57 mm and Pseudomonas aeruginosa with zone of inhibition 10±0.25mm. GC-MS analysis of leaf extract revealed presence of 14 prominent chemical compounds. Among them, major abundant compounds includes pentadecanoic acid, phytol, cis, cis, cis-7, 10, 13-hexadecatrienal Hexadecanoic acid, 2-hydroxy- 1-(hydroxymethyl) ethyl ester, γ-Sitosterol and Lupeol. Five compounds such as D-chiro-Inositol 3-O-(2-amino-4-((carboxyiminomethyl) amino)-2, 3, 4, 6-tetradeoxy-.alp, phytol, cis, cis, cis-7, 10, 13-hexadecatrienal, vitamin E acetate and β-sitosterol were associated with antioxidant property and compounds like 1, 2, 3-propanetriol monoacetate, octadecanoic acid, phytol, 2,6,10,14,18,22-Tetracosahexaene, and 2,6,10,15,19,23-hexamethyl-, (all-E) were known to posesss antimicrobial activity. Several other compounds with a number of therapeutic properties were also reported in the present study.

Identification of Bioactive Compounds of Leaf Extracts of Sida cordata (Burm.f.) Borss.Waalk. by GC/MS Analysis.

Sida cordata (Burm.f.) Borss.Waalk. is a perennial prostrate herb belonging to the family Malvaceae found in the tropical and subtropical plains of India, Sri Lanka and Pakistan. It is one of the most essential plants in Ayurveda and Siddha system of traditional medicine and is used to treat various diseases and ailments. The present study is carried out to investigate the preliminary phytochemicals and bioactive compounds of leaf extracts by GC-MS. GC-MS analysis of leaf extracts revealed the presence of 50 compounds in all four solvents. Petroleum ether extract showed the presence of 18 compounds, whereas ethanol extract showed 12 compounds; chloroform and acetone extracts showed the presence of 10 compounds each. Phytol is the most significant compound in petroleum ether (28.72%), ethanol (32.99%), and acetone (41.61%) extracts. Whereas in chloroform extract, 1,2-benzenedicarboxylic acid (49.07%) is the most significant bioactive compound having antioxidant, antifouling and antimicrobial properties.

Phytochemical Evaluation, FT-IR and GC-MS Analysis of Leaf Extracts of Pergularia daemia

Phytochemical Evaluation, FT-IR and GC-MS Analysis of Leaf Extracts of Pergularia daemia

Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici

ABSTRACT: The present study was performed to evaluate antifungal activity and GC-MS analysis of leaf extract of Chenopodium mural against Fusarium oxysporum f. sp. lycopersici (FOL), a highly problematic soil-borne pathogen of tomato. Dried leaves of C. murale were extracted with methanol for 2 weeks and after evaporating the solvent on a rotary evaporator, antifungal bioassay was carried out against FOL. All concentrations (1 to 5%) significantly reduced FOL biomass by 14-45%. The remaining methanolic extract was fractioned with n-hexane, chloroform and ethyl acetate and all these fractions were assayed for their antifungal potential. A 200 mg mL-1 concentration of various sub-fractions reduced fungal biomass significantly by 94-98% over control. All the sub-fractions were subjected to GC-MS analysis that revealed presence of 32 compounds in n-hexane, 2 compounds in chloroform and 13 compounds in ethyl acetate sub-fraction. The predominant compounds in n-hexane sub-fraction were hexadecanoic acid, methyl ester (14.64%), methyl linolenate (16.61%) and g-sitosterol (13.53%). In chloroform sub-fraction, bis (2-ethylhexyl) phthalate (92.31% and in ethyl-acetate sub-fraction, ethyl butyrate (19.57%), dihexyl phthalate (11.19%) and dioctyl phthalate (12.16%) were present in higher concentration.

Identification of Secondary Metabolite Compounds in Kirinyuh (Chloromelaena odorata) Leaf as Organic Pesticides for Wide Leafy Vegetables

This study was conducted to determine the potensial of the leaves Kirinyuh (Chloromelaena Odorata) as pesticides and phytohormones on vegetable-leaved mustard greens (Barissica juncea). Concentration of ethanol extract of leaves Kirinyuh (Chloromelaena odorata) is 5, 10, and 15 %, and 0% as control. This study uses the full tool design (RAL) of data in regression and Anova analysis followed by BNT 5%. Kirinyuh leaf extract (Chloromelaena odorata) has the activity of pestisides on soil worm (Agrotis Ipsilon) and growth hormone on mustard plants. Various concentrations of ethanol extract given a different effect on the death rate toward gound caterpillar (Agrotis Ipsilon). For the analysis of variants that influence leaf extract concentration Kirinyuh (Chloromelaena odorata) with ethanol to death worm ground (Agrotis Ipsilon) showed that very influential or significant to mortality rates with the smallest Significant Difference Test (BNT) 1 % (0,01) = 0.89 and 5% (0.05) = 0.59, while different rotational states significant differences (significant) between each treatment in which a concentration of 10 and 5 % respectively differently 2.533 (a) as well as the concentration of 15 and 10% ie 4.083 (b) as well as 15 and 5% ie 5.050 (c). Chromatogram GCMS analysis of leaf extract of Kirinyuh yielded 2 peak compounds with prediction of Octadekatriknoik acid compounds and compounds with the main framework of Hexadekanoic Acid. This compounds as compounds organic pesticides.

Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells

Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity.

GC-MS analysis of leaf extracts of Terminalia macroptera and Dioclea reflexa, two medicinal plants used for the treatment of respiratory tract disorders

GC-MS analysis of leaf extracts of Terminalia macroptera and Dioclea reflexa, two medicinal plants used for the treatment of respiratory tract disorders

Attraction of adult sweet potato weevils,Cylas formicarius elegantulus (Summers), (Coleoptera: Curculionidae), to sweet potato leaf and root volatiles

A dual-choice olfactometer was developed to study the responses of sweet potato weevils,Cylas formicarius elegantulus (Summers), to volatiles from the sweet potato,Ipomoea batatas (L.) Lam. Both males and females were attracted by volatiles from sweet potato leaves and a methylene chloride leaf extract. Females, but not males, responded to volatiles from storage roots and a methylene chloride root extract. Leaves and storage roots from four sweet potato cultivars (Centennial, Jewel, Resisto, and Regal) were attractive to female weevils; however, the attractant response varied with cultivar. GC profiles from leaf and root extracts, and GC-MS analysis of leaf extract, for Jewel cultivar enabled the volatile peaks to be identified as sesquiterpenes.