Gastric Wound Healing Research Articles

Combination of a pH-responsive peptide amphiphile and a conventional antibiotic in treating Gram-negative bacteria

BackgroundClinical treatments ofgastric infections using antibiotics suffer from the undesired killing of commensal bacteria and emergence of antibiotic resistance. It is desirable to develop pH-responsive antimicrobial peptides (AMPs) that kill pathogenic bacteria such as H. pyloriand resistant E. coli under acidic environment with minimal impact to commensal bacteria whilst not causing antibiotic resistance. ExperimentsUsing a combined approach of cell assays, molecular dynamics (MD) simulations and membrane models facilitating biophysical and biochemical measurements including small angle neutron scattering (SANS), we have characterized the pH-responsive physiochemical properties and antimicrobial performance of two amphiphilic AMPs, GIIKDIIKDIIKDI-NH2 and GIIKKIIDDIIKKI-NH2 (denoted as 3D and 2D, respectively), that were designed by selective substitutions of cationic residues of Lys (K) in the extensively studied AMP G(IIKK)3I-NH2 with anionic residue Asp (D). FindingsWhilst 2D kept non-ordered coils across the entire pH range studied, 3D displayed a range of secondary structures when pH was shifted from basic to acidic, with distinct self-assembly into nanofibers in aqueous environment. Further experimental and modeling studies revealed that the AMPs interacted differently with the inner and outer membranes of Gram-negative bacteria in a pH-responsive manner and that the structural features characterized by membrane leakage and intramembrane nanoaggregates revealed from fluorescence spectroscopy and SANS were well linked to antimicrobial actions. Different antimicrobial efficacies of 2D and 3D were underlined by the interplay between their ability to bind to the outer membrane lipid LPS (lipopolysaccharide), outer membrane permeability change and inner membrane depolarization and leakage. Furthermore, AMP’s binding with the inner membrane under acidic condition caused both the dissipation of membrane potential (Δψ) and the continuous dissipation of transmembrane ΔpH, with Δψ and ΔpH being the key components of the proton motive force. Combinations of antibiotic (Minocycline) with the pH-responsive AMP generated the synergistic effects against Gram-negative bacteria only under acidic condition. These features are crucial to target applications to gastric infections, anti-acne and wound healing.

Ultrafast self-gelling, sprayable, and adhesive carboxymethyl chitosan/poly-γ-glutamic acid/oxidized dextran powder for effective gastric perforation hemostasis and wound healing

The rapid and effective hemostasis of gastrointestinal bleeding sites remains an urgent clinical challenge. In this study, an ultrafast self-gelling, sprayable, and adhesive carboxymethyl chitosan/poly-γ-glutamic acid/oxidized dextran (CPO) powder was designed for gastric perforation hemostasis and healing. When the CPO powder was sprayed to the gastric perforation site, the CPO powder absorbed water from the blood and concentrate blood cells and clotting factors to achieve the purpose of rapid hemostasis. During the hemostasis, the CPO powder formed a hydrogel in situ through the formation of amide bonds and Schiff base bonds within 15 s, forming a physical barrier to cover the wound surface. Concurrently, the aldehyde group (-CHO) of oxidized dextran formed additional Schiff base bonds with the amino group (−NH2) of the tissue, enabling the CPO powder with wound surface adhesion. Moreover, the CPO powder was shown to have excellent in vitro and in vivo antibacterial properties and it was able to promote the healing of infected wounds in a mouse model. In summary, CPO powder provides a promising idea for the rational design of gastrointestinal hemostatic agents.

Honokiol, an inducer of sirtuin-3, protects against non-steroidal anti-inflammatory drug-induced gastric mucosal mitochondrial pathology, apoptosis and inflammatory tissue injury.

Mitochondrial oxidative stress, inflammation and apoptosis primarily underlie gastric mucosal injury caused by the widely used non-steroidal anti-inflammatory drugs (NSAIDs). Alternative gastroprotective strategies are therefore needed. Sirtuin-3 pivotally maintains mitochondrial structural integrity and metabolism while preventing oxidative stress; however, its relevance to gastric injury was never explored. Here, we have investigated whether and how sirtuin-3 stimulation by the phytochemical, honokiol, could rescue NSAID-induced gastric injury. Gastric injury in rats induced by indomethacin was used to assess the effects of honokiol. Next-generation sequencing-based transcriptomics followed by functional validation identified the gastroprotective function of sirtuin-3. Flow cytometry, immunoblotting, qRT-PCR and immunohistochemistry were used measure effects on oxidative stress, mitochondrial dynamics, electron transport chain function, and markers of inflammation and apoptosis. Sirtuin-3 deacetylase activity was also estimated and gastric luminal pH was measured. Indomethacin down-regulated sirtuin-3 to induce oxidative stress, mitochondrial hyperacetylation, 8-oxoguanine DNA glycosylase 1 depletion, mitochondrial DNA damage, respiratory chain defect and mitochondrial fragmentation leading to severe mucosal injury. Indomethacin dose-dependently inhibited sirtuin-3 deacetylase activity. Honokiol prevented mitochondrial oxidative damage and inflammatory tissue injury by attenuating indomethacin-induced depletion of both sirtuin-3 and its transcriptional regulators PGC1α and ERRα. Honokiol also accelerated gastric wound healing but did not alter gastric acid secretion, unlike lansoprazole. Sirtuin-3 stimulation by honokiol prevented and reversed NSAID-induced gastric injury through maintaining mitochondrial integrity. Honokiol did not affect gastric acid secretion. Sirtuin-3 stimulation by honokiol may be utilized as a mitochondria-based, acid-independent novel gastroprotective strategy against NSAIDs.

Gastric alarmin release: A warning signal in the development of gastric mucosal diseases.

Alarmins exist outside cells and are early warning signals to the immune system; as such, alarmin receptors are widely distributed on various immune cells. Alarmins, proinflammatory molecular patterns associated with tissue damage, are usually released into the extracellular space, where they induce immune responses and participate in the damage and repair processes of mucosal diseases.In the stomach, gastric alarmin release has been shown to be involved in gastric mucosal inflammation, antibacterial defense, adaptive immunity, and wound healing; moreover, this release causes damage and results in the development of gastric mucosal diseases, including various types of gastritis, ulcers, and gastric cancer. Therefore, it is necessary to understand the role of alarmins in gastric mucosal diseases. This review focuses on the contribution of alarmins, including IL33, HMGB1, defensins and cathelicidins, to the gastric mucosal barrier and their role in gastric mucosal diseases. Here, we offer a new perspective on the prevention and treatment of gastric mucosal diseases.

Ascidian-inspired aciduric hydrogels with high stretchability and adhesiveness promote gastric hemostasis and wound healing.

Adhesives for gastric hemorrhage are of great clinical significance. However, it remains a major challenge in clinics due to its poor stability under acidic environments and low adhesion to wet tissues. Herein, inspired by the high adhesiveness of the ascidian secretory protein, we designed a series of aciduric bionic hydrogel adhesives (PDTAs) based on poly(γ-glutamic acid) (γ-PGA) and tannic acid (TA). The formation of hydrogel adhesives was attributed to the abundant hydrogen bonds between amide groups of PGA-DA and polyphenol groups of TA. These hydrogel adhesives exhibited enhanced wet tissue adhesion (400%), higher stretchability (800% elongation), and aciduric stability (7 days) compared with commercial fibrin glue. Rodent wound models indicated that the hydrogel adhesives demonstrated significant healing promotion due to ameliorating collagen deposition and angiogenesis. These hydrogel adhesives show great potential in treating gastric hemorrhages and promoting wound healing.

Comparison of absorbable barbed suture devices versus polyglactin 910 sutures in double-layer gastrotomy closure in A canine model

Objectives: To evaluate the safety and efficacy of a commercially available absorbable barbed suture device in double-layer gastrotomy closure in a dog model. Methods: In this experimental comparative study a gastrostomy was performed in 12 adult healthy mongrel dogs (divided into two groups A and B). The stomach was closed using polyglactin 910 (Vicryle, Ethicon) suture in group A and using absorbable barbed suture (Stratafex, Ethicon) in group B. Results: Polyglactin 910 suture produced a more inflammatory reaction around the wound which caused a delay in gastric wound healing and gastric motility as well as gastric emptying time. The ultrasonographic examination revealed a significant increase in gastric wall thickness in group A compared to group B and a highly significant decrease of gastric contraction in group A in comparison to group B. Gastric closure time was significantly shorter in group B than in group A. No postoperative complications or deaths were observed in both groups. Conclusion: Absorbable barbed suture was recommended in gastric surgery as it is minimizing the time of gastric closure and optimizing the healing of the wound with faster returning of the stomach to its normal function.

Injectable Self-Healing Adhesive pH-Responsive Hydrogels Accelerate Gastric Hemostasis and Wound Healing

HighlightsA series of novel injectable pH-responsive self-healing hydrogels with enhanced adhesive strength were prepared.The hydrogels showed good gastric hemostasis property in a swine gastric hemorrhage model.The hydrogels greatly enhanced gastric wound healing in a swine gastric wound model.

LncRNA HOTAIR knockdown suppresses gastric cancer cell biological activities.

The aim of this study was to characterize the involvement of long noncoding RNA HOTAIR in gastric cancer development. Measurement of HOTAIR and miRNA‐206 expression by in situ hybridization (ISH) and analyzed for the correlation between HOTAIR and miRNA‐206 in gastric cancer tissues. To evaluate the effects of HOTAIR in gastric cancer, MTT assay, flow cytometry, transwell, and wound healing assays were applied. To explain the mechanism behind HOTAIR's involvement, the expression of proteins related to it was also measured by Western blotting. Finally, correlations among related factors were determined by a luciferase target experiment. HOTAIR expression significantly increased, and miRNA‐206 expression significantly decreased in cancer tissues (p < .01 and p < .001, respectively); HOTAIR knockdown suppressed cell viability, increased cell apoptosis by maintaining cells in the G1 phase, and inhibited cell invasion and migration by regulating miRNA‐206 expression (p < .01 or p < .001). Meanwhile, with HOTAIR knockdown, CCND1 and CCND2 protein expressions were significantly suppressed, whereas miRNA‐206 expression increased (p < .01 or p < .001). HOTAIR was shown to target miRNA‐206 and miRNA‐206 targeted CCND1 and CCND2. HOTAIR knockdown had antitumor effects by suppressing CCND1 and CCND2 expression by stimulating miRNA‐206 in gastric cancer in vitro study.

A-kinase-interacting protein 1 facilitates growth and metastasis of gastric cancer cells via Slug-induced epithelial-mesenchymal transition.

A‐kinase‐interacting protein 1 (AKIP1) has previously been reported to act as a potential oncogenic protein in various cancers. The clinical significance and biological role of AKIP1 in gastric cancer (GC) is, however, still elusive. Herein, this study aimed to investigate the functional and molecular mechanism by which AKIP1 influences GC. AKIP1 mRNA and protein expressions in GC tissues were examined by quantitative real‐time PCR (qRT‐PCR), Western blot and immunohistochemistry. Other methods including stably transfected against AKIP1 into gastric cancer cells, wound healing, transwell assays, CCK‐8, colony formation, qRT‐PCR and Western blot in vitro and tumorigenesis in vivo were also performed. The up‐regulated expression of AKIP1 in GC specimens significantly correlated with clinical metastasis and poor prognosis in patients with GC. AKIP1 knockdown markedly suppressed GC cells proliferation, invasion and metastasis both in vitro and in vivo. In contrast, AKIP1 overexpression resulted in the opposite effects. Moreover, mechanistic analyses indicated that Slug‐induced epithelial‐mesenchymal transition (EMT) might be responsible for AKIP1‐influenced GC cells behaviour. Our findings demonstrated that high AKIP1 expression significantly correlated with clinical metastasis and unfavourable prognosis in patients with GC. Additionally, AKIP1 promoted GC cells proliferation, migration and invasion by activating Slug‐induced EMT.

Seabuckthorn (Hippophae L.) technologies for environmental conservation and development of value chain in Himachal Pradesh

In Himachal Pradesh, cold deserts, comprising about 42% of total geographical area of the state, occur in districts of Lahaul-Spiti, upper parts of Chamba and Kinnaur in Himachal Pradesh state. the region is characterized by extreme climatic conditions, high rates of soil erosion and land slides, shortage of fuel wood, timber and fodder, low productivity of agricultural lands and sparse vegetation and lack of job opportunities. Plantation forests of willow are also dying in several places due to large scale pest attack. Lack of job opportunities in this strategic remote border region have led to youths to migrate to other parts of the state and country. the overall size of holding of the farmers was 1.3 ha in Lahaul, the region present studies were carried out on seabuckthorn. Most of the farmers in the region are marginal. 86 per cent of the gross income was provided by two crops potato and pea. there is a stagnation in the market of potato and pea is badly affected by powdery mildew, besides increasing cost of input of both crops. Before the start of the project, some farmers used to collect seabuckthorn fruit occasionally from seabuckthorn forest and sell to traders at low price (Rs.20/kg) with out any market assurance. In order to develop appropriate technologies and a model for seabuckthorn resource development and their commercial utilization, 11 research projects on seabuckthorn besides departmental studies since 1993, were completed in CSK HPKV, Palampur. However, the major project was ICAR funded NAIP sub-project entitled ‘A value chain on seabuckthorn”, sanctioned in June 2008 for about Rs. 4.11 crores for a duration of 2008–2014, with an aim to develop a model on value chain on seabuckthorn with linkages with R&D organizations, growers, NGos and market assurance, based on production to consumption system. the main R&D centre (CSK HPKV, Palampur) created awareness about the project and economic potential of seabuckthorn, providing training and extension service to about 500 farmers in modern cultivation technologies and mass propagate (245,000 saplings) of improved planting material of seabuckthorn, improve productivity (from 2 tons fruit/ha to 5 tons/ha) and production through providing high yielding seabuckthorn selections (2–3 land lines), improvement of agrotechniques, development of fruit harvesters (5) and cultivation of seabuckthorn by 400 farmers in 120 ha marginal lands in Lahaul. HPKV scientists standardized various value added food 16), animal and poultry feed (7), oil and leaf preparations (5) for the treatment of gastric ulcer, wound healing and skin disease, whereas AIIMS standardized the seabuckthorn fruit pulp oil for cardioprotection. University carried survey for the acceptability of value added seabuckthorn products in the market and analyzed the economic viability of the seabuckthorn programme. the studies are being carried out on 14 Russian seabuckthorn to domesticate the 2–3 most suitable for the local conditions. Studies also found that seabuckthorn plays an important role in conservation of biodiversity and soil improvement. A number of industries have come up in the state, producing a range of seabuckthorn products.

TO STUDY THE EFFECTS OF DIFFERENT STRENGTHS OF DEXMEDETOMIDINE INFUSION ON HEMODYNAMIC RESPONSES IN LAPAROSCOPIC CHOLECYSTECTOMY

Background: Laparoscopic cholecystectomy is a minimally invasive procedure gaining popularity in the recent years. Open cholecystectomy procedures are more invasive with prolonged recovery, increased analgesic requirement, delayed gastric recovery and wound healing and increased pulmonary complications. Laparoscopic surgeries provide major benefits with faster recovery time, reduced postoperative pain and reduced hospital stay. Laparoscopic surgeries, require the creation of pneumoperitoneum using carbon dioxide along with patient positioning (Trendelenburg or reverse Trendelenburg) which causes physiologic changes which can be deleterious to patients with preexisting diseases. The changes occurring during laparoscopy can be attenuated using various drugs like vasodilating agents, alpha2 adrenergic agonists, opioids and beta blocking agents. This study was done to compare the efficacy of low dose infusion of dexmedetomidine using different strengths on attenuating the hemodynamic responses occurring in laparoscopic cholecystectomy. Sixty patients between 18 and 60 years of either sex belonging to ASAMethodology: grade 1 and 2 scheduled for elective laparoscopic cholecystectomy under general anaesthesia were randomly allotted to one of the two groups of 30 each. Group DEX 0.2 received Dexmedetomidine at 0.2mcg/kg/hour and Group DEX 0.4 received Dexmedetomidine at 0.4mcg/kg/hour 15 minutes prior to induction. Haemodynamic variables were recorded at baseline, post intubation, aftercreation of pneumoperitoneum and after extubation. Other parameters noted were VAS score, RSS score and time to first supplementation of rescue analgesic. In both the groups there was a rise in theResults: mean arterial pressure and heart rate post intubation and after creation of pneumoperitoneum from the baseline. But the rise was considerably lower in DEX 0.4 group. After 30 minutes of pneumoperitoneum, there was a considerable fall in the heart rate and mean arterial pressure in the DEX 0.4 group. There was no difference in the time for extubation in both the groups. The postoperative analgesic requirements were lesser in DEX0.4 group. No significant side effects were noted. Low dose dexmedetomidine as an infusion started 15 minutes prior to induction does notConclusion: completely attenuate the stress response to intubation and pneumoperitoneum. In comparison, o.4mcg/kg/hour of Dexmedetomidine provides better response than 0.2mcg/kg/hour.

Tissue thrombin is associated with the pathogenesis of dilated cardiomyopathy

BackgroundThrombin is a serine protease known to be the final product of the coagulation cascade. However, thrombin plays other physiological roles in processes such as gastric contractions and vessel wound healing, and a state of coagulability is increased in patients with dilated cardiomyopathy (DCM). In this study, we investigate the role of thrombin in the pathogenesis of DCM. The purpose of this study is to clarify the role of thrombin in the pathogenesis of DCM and investigate the possibility of treatment against DCM by thrombin inhibition. MethodsWe investigated the expression of thrombin in the left ventricles of five patients with DCM who underwent the Batista operation and four patients without heart disease. Furthermore, we investigated the involvement of thrombin in the development of DCM using knock-in mice with a deletion mutation of cardiac troponin T that causes human DCM (∆K210 knock-in mouse) (B6;129-Tnnt2tm2Mmto) and assessed the effects of a direct thrombin inhibitor, dabigatran on ∆K210 knock-in mice using echocardiographic examinations, the Kaplan-Meier method and Western blotting. ResultsThe immunohistochemical analysis showed a strong thrombin expression in the DCM patients compared to the patients without heart disease. In immunohistochemical analysis, a strong thrombin expression was observed in the heart tissues analysis in the ∆K210 knock-in mice. Dabigatran administration significantly improved fractional shortening according to the echocardiographic examination and the survival outcomes in ∆K210 knock-in mice. ConclusionTissue thrombin is involved in the pathogenesis of DCM and thrombin inhibition can be beneficial for the treatment of DCM.

Carbon monoxide promotes gastric wound healing in mice via the protein kinase C pathway

Previous studies have shown that carbon monoxide (CO) is involved in a variety of physiological and pathophysiological processes including anti-inflammatory, anti-apoptotic and anti-oxidant responses. However, it remains unclear whether CO promotes gastric ulcer healing. In the present study, we evaluated the efficacy of CO-saturated saline in the treatment of gastric ulcers and its underlying mechanism. Acute gastric ulcers were induced in C57BL/6 male mice using acetic acid. A CO-saturated solution was prepared by bubbling 50% CO gas into saline. To investigate the effect of CO on gastric mucosal healing, CO solution was orally administrated twice a day beginning on day 3 after the induction of gastric ulcer. Mice were sacrificed on day 7 after ulcer induction. The stomach was removed, and the ulcerated lesions were measured. In vitro wound healing assays were used to determine the mechanism of action of CO in the restoration of murine gastric epithelial cells. The oral administration of CO solution accelerated the gastric ulcer healing by promoting re-epithelialization. Furthermore, the wound healing assay performed using the murine gastric epithelial cells revealed that the CO-saturated medium enhanced cell migration through the activation of protein kinase C (PKC). Based on these data, CO may represent a novel therapeutic approach for the treatment of gastric mucosal injuries.

Mo1382 Peri-Operative Administration of Resveratrol Did Not Hinder Abdominal or Gastric Wound Healing in a Murine Model

Mo1382 Peri-Operative Administration of Resveratrol Did Not Hinder Abdominal or Gastric Wound Healing in a Murine Model

Functional expression of recombinant human trefoil factor 1 by Escherichia coli and Brevibacillus choshinensis.

BackgroundTrefoil factor 1 (TFF1) mediates mucosal repair and belongs to a highly conserved trefoil factor family proteins which are secreted by epithelial cells in the stomach or colon mucous membrane. TFF1 forms a homodimer via a disulphide linkage that affects wound healing activity. Previous recombinant expressions of TFF1 were too low yield for industrial application. This study aims to improve the expression level of bioactive recombinant TFF1 (rTFF1) and facilitate application potency.MethodsThe rTFF1 gene rtff1 was synthesized, expressed by Escherichia coli and secreted by Brevibacillus choshinensis. The rTFF1s were purified. The polymeric patterns and wound healing capacities of purified rTFF1s were checked.ResultsIn Escherichia coli, 21.08 mg/L rTFF1 was stably expressed as monomer, dimer and oligomer in soluble fraction. In Brevebacillus choshinensis, the rTFF1 was secreted extracellularly at high level (35.73 mg/L) and formed monomer, dimer and oligomer forms. Both proteins from different sources were purified by Ni-NTA chromatography and exhibited the wound healing activities. The rTFF1 produced by B. choshinensis had better wound healing capability than the rTFF1 produced by E. coli. After pH 2.4 buffer treatments, the purified rTFF1 formed more oligomeric forms as well as better wound healing capability. Glycosylation assay and LC-MS/MS spectrometry experiments showed that the rTFF1 produced by B. choshinensis was unexpectedly glycosylated at N-terminal Ser residue. The glycosylation may contribute to the better wound healing capacity.ConclusionsThis study provides a potent tool of rTFF1 production to be applied in gastric damage protection and wound healing. The protein sources from B. choshinensis were more efficient than rTFF1 produced by E. coli.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-015-0149-5) contains supplementary material, which is available to authorized users.

Does a muscle flap accelerate wound healing of gastric wall defects compared with an omental flap?

IntroductionMost often used for reconstruction at superficial sites, a muscle flap recently was reported to promote clinical wound healing in a duodenal defect. We therefore examined whether a muscle flap could promote wound healing comparably to an omental flap in rats with gastric wall defects. MethodsAfter perforation of the centre of the anterior gastric wall, rats were divided into 2 groups. In the muscle group, a muscle flap was fixed to the defect; in the omentum group, an omental flap was placed over the defect. We histopathologically compared tissue responses during gastric wall healing. ResultsWhile stratified villi had completely covered the defect by day 7 in both groups, scar maturation differed. Scar tissue persisted in the muscle group, but was gradually replaced by adipose tissue in the omentum group. DiscussionBoth muscle and omental flaps accelerated gastric wall wound healing. ConclusionA muscle flap is an excellent alternative for repair of gastric defects when no omental flap is available.

Effects of liposome-encapsulated hemoglobin on gastric wound healing in the rat.

Liposome-encapsulated hemoglobin (LEH) may improve microcirculation and oxygen (O2 ) metabolism at a surgical wound to accelerate its healing. Ten mL/kg of LEH with high (h-LEH) or low O2 -affinity (l-LEH), homologous red blood cells (RBC), empty liposome or saline as a control was infused before a 10-mm incision and interrupted suture closure of the gastric wall in a total of 110 rats. Two and 4 days later, the stomach was excised for bursting pressure determination and histological sampling. The dose-response relationship was examined in 70 additional rats receiving progressively reduced doses of h-LEH. Hypoxia-inducible factor-1α (HIF-1α) was stained immunohistochemically in 54 other rats to examine its accumulation at the anastomotic sites. Bursting pressure of the surgical wound was significantly higher 2 days after surgery only in the h-LEH-treated rats (P < 0.05), but not at 4 days after surgery, when other rats showed increased bursting pressure to a nonsignificant level. Histological examination revealed less granulocyte infiltration, better granulation, and more macrophage infiltration in h-LEH-treated rats at 2 days, but no longer at 4 days postsurgery. Dose-response study revealed that 0.4 mL/kg of h-LEH (hemoglobin 24 mg/kg) was effective for elevating bursting pressure at 2 days. h-LEH-treated rats had significantly suppressed HIF-1α accumulation in the wound 6, 24, and 48 h after surgery as compared with control animals treated with homologous RBC or saline. In conclusion, the results suggest that h-LEH, but not l-LEH or homologous transfusion, may accelerate wound healing early after gastric incision and anastomosis in the rat. The mechanism(s) appears to be related to improved O2 supply, aerobic metabolism, and suppressed inflammation in the wound.

Efeito de Schinus terebinthifolius Raddi (aroeira) e Carapa guianensis Aublet (andiroba) na cicatrização de gastrorrafias

The gastrorraphy isolated or associated with the use of biological adhesives formed throughout the history of surgery the usual way to promote healing in gastric lesions; however, the use of herbal medicine has been increasingly employed to help the wound healing. To evaluate the wound healing caused in the stomach of rats using extract of Schinus terebinthifolius Raddi and Carapa guianensis Aublet oil. Ninety rats, adult males were divided into three groups: aroeira, andiroba and control group, which were subdivided into three subgroups of five animals according to the time of the deaths (seven, 14 and 21 days). All underwent the same surgical procedure (injury and suture the stomach) differing only to the animals in groups aroeira and andiroba that received a daily dose of 100 mg / kg of hydroalcoholic extract and oil, by gavage, while the control group received normal saline. The parameters evaluated were the macroscopic and microscopic test of resistance to air insufflation and test the traction force. All animals showed good healing of gastric and abdominal wall without infection and dehiscence. Both groups presented neighboring organs adhesions on the gastric surface. The endurance test for air insufflation showed higher average pressure within seven days and the test revealed greater traction force of rupture between seven and 14 days in groups aroeira and andiroba. The intensity of chronic inflammation revealed statistically significant differences in angiogenesis and fibroblast proliferation. The use of extract of Schinus terebinthifolius Raddi and Carapa guianensis Aublet oil favored the gastric wound healing in rats.

Perioperative polyphenon E- and siliphos-inhibited colorectal tumor growth and metastases without impairment of gastric or abdominal wound healing in mouse models

Perioperative anticancer therapy that does not impair wound healing is needed to counter the persistent proangiogenic plasma compositional changes that occur after colorectal resection. Polyphenon E (PolyE), a green tea derivative (main component EGCG), and Siliphos (main component silibinin), from the milk thistle plant, both have antitumor effects. This study assessed the impact of PolyE/Siliphos (PES) on wound healing and the growth of CT-26 colon cancer in several murine models. One wound healing and three tumor studies were performed. Tumor Study (TS)1 assessed the impact of PES on subcutaneous tumor growth, whereas TS2 assessed PES's impact on subcutaneous growth when given pre- and post-CO(2) pneumoperitoneum (pneumo), sham laparotomy, or anesthesia alone. TS3 determined the ability of PES to limit hepatic metastases (mets) after portal venous injection of tumor cells. In the final study, laparotomy and gastrotomy wound healing were assessed several ways. BALB/c mice were used for all studies. The drugs were given via drinking water (PolyE) and gavage (Siliphos), daily, for 7-9days preprocedure and for 7-21days postoperatively. Tumor mass, number/size of hepatic mets, and proliferation and apoptosis rates were assessed. The abdominal breaking strength and energy to failure were measured postmortem as was gastric bursting pressures. PES significantly inhibited subcutaneous growth in the nonoperative setting. PES also significantly decreased the number/size of liver mets when given perioperatively. Abdominal wound breaking strength, energy to wound failure, and collagen content were not altered by PES; gastrotomy bursting strength also was not affected by PES. Neither drug alone had a significant impact on tumor growth. The PES combination inhibited subcutaneous and hepatic tumor growth yet did not impair wound healing. PES holds promise as a perioperative anticancer therapy.

W1950 A Combined Oral Regimen of EGCG and Silibinin Inhibited the Subcutaneous Growth of the CT-26 Colon Cancer Cell Line and Had NO Adverse Effect on Abdominal or Gastric Wound Healing

W1950 A Combined Oral Regimen of EGCG and Silibinin Inhibited the Subcutaneous Growth of the CT-26 Colon Cancer Cell Line and Had NO Adverse Effect on Abdominal or Gastric Wound Healing