Animal Crude Gastrin has been a major source of gastrin for gastric acid stimulant in experimental studies. The objective of this study was to evolve a method of crude gastrin purification that could yield a better gastric acid output. The Gastrin was extracted by the standard method of Gregory and tracy(1964) nodified by Adelaiye and Amure(1990)Extraction and purificatio of Crude Gastrin has been a physiological problem since Edkins first extracted Gastrin from the antral mucosa of animals (Edkins 1905) in 1954 Tauber and Medison described a new method for gastrin purification using the cell gel chromotography process. In this experiemnt crude gastrin extract was obtained from the antrum of the Camel, Sheep and Goat and purified using Sephadex G 50. The crdue extract solution was passed through Sephadex G50( Upsala Phamacia) column chromotography. The effluent was then adminsistered to albino rats in at O.5ml. The basal gastric acid output rose from 0.23 meq/lt to 2.32meq/l in 20minutes in sephadex purified crude gastrin as compared to 0.23 to 0.27 meq/lit for the same period. This was found to be statististically significant P<0.02.Sephadex G50 column chromotography purifies crude gastrin extract and improves the gastric stimulant affect.