A ventilation system with a constant velocity air supply might form airflow stagnant zones in industrial plants, making it challenging to dilute hazardous gas effectively. In this study, a novel ventilation mode called pulsating ventilation, which can potentially dilute the hazardous gas better, was applied in a ventilated room with nozzle air supply. Three different scenarios of gas accumulation were assumed, with the location of gas accumulation situated at the rear, near the floor, and in the corners of the room. Both the distribution of CO2 concentration and the efficiency of reducing the indoor CO2 concentration to the safety threshold were analyzed to evaluate the ventilation performance of pulsating ventilation. The results showed that pulsating ventilation outperformed constant velocity air supply in expeditiously eradicating gas accumulation areas for equivalent total air volumes. When the gas accumulation areas were located at the rear of the room, close to the floor, and in the corner of the room, pulsation ventilation reduced the time for the indoor CO2 concentration to drop to the safety threshold by 36.19%, 27.23%, and 36.25%, respectively. Holding the period constant, an increased amplitude correlated with a swifter decrease in peak pollutant concentration. Except for the case where the gas accumulation areas are close to the floor, when the amplitude is constant, the larger the period, the faster the peak concentration in the gas accumulation areas decreases.