Garut Ram Research Articles

Comparison Enzymatic and Non-Enzymatic Antioxidant in Sperm Quality of Garut Ram Chilled Semen to Enhance Rural Livestock Commodity

Comparison Enzymatic and Non-Enzymatic Antioxidant in Sperm Quality of Garut Ram Chilled Semen to Enhance Rural Livestock Commodity

Optimizing Reproductive Performances of Garut Sheep as Superior Local Genetic Resources by Modification of Dietary Grass and Concentrate Balances

High-quality Garut rams are important to be selected for Garut sheep development to include improving their semen qualities. The semen quality is highly affected by dietary nutrient intake. This research aimed to conclude the effect of different grass and concentrate balances on performance and semen qualities such as motility, abnormality, the integrity of plasma membrane, and acrosome cap sperms of Garut rams. This research was conducted from August 25th, 2020, to February 2nd, 2021, at Margawati Sheep Breeding Center. A Completely Randomized Design (CRD) was used to compare 3 different grass and concentrate balance treatments (T1 = 80% grass: 20% concentrate; T2 = 60% grass: 40% concentrate; and T3 = 40% grass: 60% concentrate) on performance and semen qualities of Garut rams using 6 replicates (n = 6). The data were analyzed using analysis of variance and Duncan's test. The results showed there was no different among the treatments on average daily gain (ADG, g/head/day) and dry matter intake (DMI, g/head/day) but increasing level of dietary concentrate resulted in increased motility (P<0.01), reduced abnormality (P<0.05), increased integrity of plasma membrane (P<0.001) and increased acrosome cap (P<0.001) of sperms of Garut Rams. The T2 treatment resulted in an optimal grass (60%) and concentrate (40%) balance. In conclusion, increased dietary concentrate did not significantly increase the daily weight gain, but it improved the Garut ram’s semen quality.

THE PRODUCTION OF FREEZE-DRIED EGG YOLK POWDER AND ITS EFFECT ON THE QUALITY OF GARUT RAM LIQUID SEMEN

This research aims to evaluate the protective potency of freeze-dried egg yolk powder on Garut ram sperm during the liquid semen preservation process. Semen with good sperm quality was divided into three groups and diluted using the following Tris diluents: fresh egg yolk-Tris (FEY-Tris), commercial egg yolk powder-Tris (CEY-Tris), and freeze-dried egg yolk powder-Tris (DEY-Tris). Semen that had been diluted was observed every 12 hours until sperm progressive motility was 50%. Sperm quality was tested for progressive motility percentages, viability, intact plasma membrane (IPM), and intact acrosome (IA). Fresh egg yolk had a particle size of 14460.00±330.76 nm, with polydispersity index value (PI) and zeta potential (ZP) being 1.00±0.11 and -9.22±0.30 mV, respectively. The particle size of commercial egg yolk powder was 877.90±168.86 nm with PI value 0.34±0.04 and ZP-28.7±1.24 mV. The particle size of freeze-dried egg yolk powder was 1296.00±86.73 nm, with PI value 0.70±0.04 and ZP -34.5±0.64 mV. Progressive motility percentage showed that DEY-Tris diluent managed to survive to 51.11±6.06% for 168 hours, compared to FEY-Tris (51.25±6.74%) which survived for 156 hours, although no longer than CEY-Tris diluent (53.47±6.33%) which survive for 180 hours (P0.05). Higher sperm motility was also supported by viability percentage, IPM, and IA, which were all higher (P0.05). In conclusion, freeze dried egg yolk powder mixed with Tris buffer was able to maintain sperm quality during the liquid semen preservation process.

The effect of pineapple [Ananas comosus (L.) Merr.] juice in extender on Garut ram (Ovis aries L.) spermatozoa quality postcryopreservation

This study was intended to determine the effect of various concentrations of pineapple [Ananas comosus (L.) Merr.] juice in extender on garut ram (Ovis aries L.) spermatozoa quality 24 h postcryopreservation. Semen samples were collected from five garut rams once a week using an artificial vagina. The samples were diluted in Tris-egg yolk extender with pineapple juice at concentrations of 0 % (KK), 5 % (KP 1), 10 % (KP 2), 15 % (KP 3), and 20 % (KP 4). The diluted semen samples were loaded into mini straws (0.25 mL) with 50 million cells/ml dosage. Samples were equilibrated at 5 °C for 2 h, and then frozen and stored in liquid nitrogen. Parameters evaluated were motility, viability, membrane integrity, acrosome integrity, and morphological abnormality. One-way ANOVA followed by Duncan’s test showed significant differences (P < 0.05) between KK and KP 3 in the percentage of motile spermatozoa and membrane integrity. Pineapple juice at 15 % could minimize the reduction of spermatozoa quality based on the percentage of motile spermatozoa (52.19 ± 5.32 %) and membrane integrity (38.52 ± 4.85 %).

Quality of Garut Ram’s Sperm in Tris Extender Supplemented with Ethylene Diamine Tetraacetic Acid Preserved at Low Temperature (3–5°C)

Ethylenediaminetetraacetic acid (EDTA) is a chemical substance that can bind the heavy metal and other toxic compounds so it is suitable to use for protecting sperm during preservation at 3-5°C. The research aimed to evaluate the role of EDTA to maintain the quality of Garut ram sperm in low-temperature preservation. Semen was collected by an artificial vagina. Fresh semen was evaluated and divided into 3 tubes and diluted with tris extender contain 20% egg yolk (TEY) without EDTA (T1), TEY+0.01% EDTA (T2) and TEY+0.02% EDTA (T 3). Semen was evaluated both macroscopically and microscopically before preserved in a refrigerator at 3-5°C. During storage, semen was also evaluated on daily basis (four consecutive days) that included variables relate to sperm motility (SM), sperm viability (SV), sperm membrane integrity (SMI) and sperm acrosome intact (SAI). The research design used was Completely Randomized Design with three treatments and five replications. Differences between treatments were tested with the least significant difference test. The results showed that the average of each variable gained of fresh semen was 0.88mL for volume, cream for color, thick for consistency and 7 for pH. While the mass movement of sperm was +++ and sperm concentration was 3,962 million cells/mL. The average percentages of SM, SV, abnormality, SMI, and SAI were 75.00%, 86.60%, 4.80%, 84.40%, and 84.80%, respectively. On the day-4 of storage, percentages of SM, SV, SMI, and SAI in control (T1) decreased to 48.75%, 66.25%, 64.75%, and 63.5%, respectively, but still met the requirement of sperm quality for artificial insemination purposes. Moreover, there was no significant difference between T2 (45.00%, 65.00%, 63.25% and 65.25%) and T3 (40.00%, 65.67%, 60.75% and 62.50%) of those variables measured. Finally, it was concluded that the addition of EDTA in Tris extender could maintain the quality of Garut ram sperm.

The effect of siam orange (Citrus nobilis Lour.) and pineapple (Ananas comosus (L.) Merr.) juices combination in extender on Garut Ram (Ovis aries L.) spermatozoa quality post-cryopreservation

The research aimed to find out the effect of various concentrations of siam orange (Citrus nobilis Lour.) and pineapple (Ananas comosus (L.) Merr.) juices combination (1:1) in extender on garut ram (Ovis aries L.) spermatozoa quality 24 hours post-cryopreservation. Semen samples were collected from five Garut Rams once a week using an artificial vagina. The samples were diluted in Tris-egg yolk extender with siam orange and pineapple juices combination 0 %, 5 %, 10 %, 15 %, and 20 %. Diluted semen samples were loaded into mini straws (0.25 mL) with 50 million cells/ml dosage. Samples were equilibrated at 5°C for two hours, then frozen and stored in liquid nitrogen. The one factor ANOVA and continued with Duncan test showed a significant differences (P<0.05) between control and sample with 10 % juices combination in the percentage of spermatozoa motility and membrane integrity, a significant difference (P<0.05) between control and sample with 5 % along with 10 % juices combination in the percentage of spermatozoa viability. Siam orange and pineapple juices combination 10% in extender was able to minimize the reduction of spermatozoa quality post-cryopreservation based on the percentage of spermatozoa motility (68.06 ± 2.34 %), viability (41.47 ± 6.64 %) and membrane integrity (46.00 ± 5.65 %).

The effect of siam orange juice (Citrus nobilis Lour.) in extender on Garut Ram (Ovis aries L.) spermatozoa quality post-cryopreservation

The research aimed to find out the effect of Siam Orange juice in extender on Garut Ram spermatozoa quality 24 hours post-cryopreservation. Semen was collected from five rams once a week using an artificial vagina. Semen sample was diluted in Tris-egg yolk-based extender containing 0 % (KK), 5 % (KP 1), 10 % (KP 2), 15 % (KP 3) and 20 % (KP 4) Siam orange juice. Semen was packed in 0.25 ml straw with a final concentration of 50x106 spermatozoa/mL. Sperm was equilibrated at 5° C for two hours then frozen and stored in liquid nitrogen (LN2) tube. Sperm parameters, including motility, viability, membrane integrity, acrosome integrity, and abnormality, were assessed. One-way ANOVA test results showed significant differences between treatment groups on the percentage of motility, viability, and membrane integrity (p <0.05). Duncan test results indicated that KP 2 had significant differences in all treatment groups. Therefore, orange juice at 10 % concentration (KP 2) was the best concentration that was able to minimize the reduction of ram spermatozoa quality post-cryopreservation based on motility [(58.68 ± 0.68) %], viability [(59.73 ± 6.47) %], and membrane integrity [(54.87 ± 5.58) %].

Preservation of epididymal spermatozoa of garut ram in soybean lechitin-based extender

Preservation of epididymal spermatozoa of garut ram in soybean lechitin-based extender

KUALITAS SEMEN BEKU DOMBA GARUT (Ovis aries) PENAMBAHAN SUKROSA DALAM PENGENCER SEMEN KUNING

The objective of this research was to examine the effect of sucrose in improving the quality of the plasma membrane intact and sperm motility of frozen semen of Garut ram. Semen was collected using artificial vagina weekly from six mature garut rams. Immediately after initial evaluation, fresh semen was divided into four parts and diluted with Tr s extender without sucrose (T0), Tris extender + sucrose 0.2g/100 ml (T1), Tris extender + sucrose 0.4g/100 ml (T2) and Tris extender + sucrose 0.6g/100 ml (T3), respectively. Results of this research showed that the percentage of sperm motility after thawing in T2 (49.00 ± 5.48%) was significantly (P&lt;0.05) higher than T0 (42.00 ± 2.74%) but was not significantly difference (P&gt;0.05) than T1 (46.00 ± 4.18%)and T3 (48.00 ± 4.47%). Evaluation of plasma membrane intact showed that T1 (62.33 ± 6.51%) was s gnificantly different (P &lt;0.05) w h T0 (49.40 ± 2.19%) but was not significantly different (P&gt; 0.05) than T2 (58.50 ± 4.97%) and T3 (56.40 ± 5.90%). In conclusion, he addition of sucrose in semen extender improved the quality of frozen semen of Garut ram. Concentration of 0.2g / 100 ml is the op ma dose to improve the quality of the plasma membrane intact and motility of spermatozoa during the freezing process.

THE PROPORTION OF X AND Y SPERM, VIABILITY AND MOTILITY OF RAM SPERMATOZOA AFTER SEPARATED WITH WHITE EGG ALBUMIN

ABSTRACT The aim of this research was to determine the proportion, viability and motility of X and Y ram spermatozoa separated with egg white albumin. Sperm samples derived from Garut ram, which was collected by using an artificial vagina. Observations were made on spermatozoa fraction above and below each medium fraction treatment. There are treatment egg white albumin as separation medium, each medium consisting of fractions top and bottom fraction with different concentration: 1) P0 = sperma before separation (control); 2) P1 = 10% above fraction + 30% lower fraction; P2 = 25% + 45%; P3 = 25% + 75%. Data proportion of X and Y, viability and motility were analyzed statistically by Completely Randomized Design patern in the direction followed by Duncan’s Multiple Range Test for data with a real difference. Separation with egg white albumin affect significantly increased the proportion of spermatozoa X and Y (P≤0.05), but tends to decrease the viability and motility of spermatozoa.The proportion of spermatozoa X and Y was highest in treatment P3,76.76% of spermatozoa X (fraction above 25%) and 79.81% spermatozoa Y (75% lower fraction), with an average viability obtained respectively 68,9% (fraction above) and 59,7% (bottom fraction), motility 77,5% (fraction above) dan 84,0% (bottom fraction). It was concluded that the egg white albumin is very effective in changing the proportions of X and Y ram sperm with the quality of spermatozoa after separation feasible for applications insemination or processed into frozen semen. (Keywords: Garut ram, White egg albumin, Spermatozoa X and Y)

Kriopreservasi Semen Domba Garut Menggunakan Tris Kuning Telur yang Disuplementasi Omega-3 Minyak Ikan Salmon (CRYOPRESERVATION GARUT SHEEP SEMEN USING TRIS EGG YOLK SUPPLEMENTED OMEGA-3 FISH OIL SALMON)

The availability of superior seeds garut sheep relatively few and the unavailability of seed supply ofsuperior male garut sheep that is continuously high productive is also a problem. . The success ofcryopreservation is influenced by the diluent being used. This study aimed was to compare the Tris EggYolk containing omega-3 (TEYO) and Tris used commercial egg yolk plus omega-3 fish oil salmon (TEYOS)to the success of cryopreservation of garut sheep semen. Five garut ram aged 1,5 – 2,0 years were used inthis study. Semen was collected using an artificial vagina, then it was evaluated and divided into twotubes, each of them was diluted with TEYO and TEYOS (50x106 sel/straw), before they then packed intostraws, equilibrated (5°C), and frozen with liquid nitrogen vapour (-130oC) for10 minutes and storedthem in the container (-196oC) for further evaluation. The results showed that post thawing values of thesperm motility, viability and intact plasma membrane were 53,75±2,46; 60,75±2,17and 72,58±2,12%respectively, diluted with TEYOS were higher (P<0,05) than those diluted with TEYOS with the spermmotility, viability and intact plasma membrane only at 41,50±1,25; 50,50±1,04 and 57,58±1,03%respectively. This result demonstrated that the recovery rate of spermatozoa in TEYOS diluent washigher (P<0,05) than TEYO. Its concluded that fish salmon oil omega-3 supplemented in TEYOS better incryopreservation of garut ram semen compared to the TEYO diluent.

Pelacakan Kerusakan Akrosom Spermatozoa Domba Selama Proses Pembekuan dengan Teknik Histokimia Lektin

Freezing process in ram spermatozoa caused damage of the plasma membrane and acrosome thatlead to the decrease of spermatozoa fertility. Research was conducted to evaluate acrosomal damageduring freezing process using lectin histochemical technique. Semen was collected twice a week usingartificial vagina from 1-2 years old Garut ram. Immediately after collection, characteristic of semenquality was evaluated then diluted with Niwa and Sasaki Freezing (NSF) medium. Semen was loadedinto 0.25 mL mini straws and equilibrated at 4oC for two hours. Straws were then frozen and stored inliquid nitrogen. Evaluation of sperm characteristic (motility, viability and plasma membrane integrity)and acrosomal integrity were done during the freezing process. Detection of acrosomal integrity was observedusing Fluorescens isothiocyanate (FITC) and Avidin-Biotin-Complex (ABC) staining methods. Data ofcharacteristic spermatozoa and acrosomal integrity were analyzed using ANOVA. Result of the experimentsshowed that the percentage of motility, viability and plasma membrane integrity of spermatozoa before freezing (83 ± 2.7%; 88.8 ± 2.6%; 88.2 ± 3.7%) were significantly decreased (P<0.05) after equilibration (71± 4.2%; 84.2 ± 5.0%; 76.2 ± 1.3%) and after thawing (40 ± 3.5%; 61.08 ± 3.3%; 51.2 ± 10.4%). The percentageof intact acrosomal spermatozoa using FITC and ABC methods during freezing process were 93.63 ±2.73%; 88.04 ± 3.2% and 81.73 ± 4.77% VS 94.54 ± 0.26%; 88.17 ± 0.38% and 79.38 ± 2.06%, respectively.In conclusion, the characteristic of spermatozoa were significantly decrease (P<0.05) during freezing process.Furthermore, the integrity of acrosome spermatozoa can be well analyzed during freezing process by usinglectin histochemical staining methods.

THE HYPO-OSMOTIC SWELLING TEST IN FRESH GARUT RAM SPERMATOZOA

The study was aimed to determine the optimal time to evaluate the maximum numbers of swollensperm after expose to hypo-osmotic solution for testing membrane integrity in fresh garut ram semen. Atotal of 24 ejaculate samples from eight sexually mature garut ram were collected using artificial vagina.After macroscopic evaluation, 10 μL undiluted semen was gently mixed in each of the 2 mL hypoosmoticsolution and incubated at 37oC for 1 hour. Two hundred of coil and non-coiled sperm wereobserved from five different fields every 15 minutes. Results demonstrated that the maximumpercentage of coiling sperm positive to HOS test was at 30 minutes and no differences among 8 garutrams used.

Mineral Utilization in Rams Fed Ration Supplemented with Different Levels of Chromium, Calcium, and Cation-Anion Balances

Chromium (Cr) is an essential mineral for ruminants. Its metabolism and interactions with other minerals has not been widely known. This experiment was designed to evaluate the utilization of minerals and growth of Garut ram fed ration supplemented with Cr and different Dietary Cation Anion Balance (DCAB) and Ca level. Dietary treatments, namely: R0 (Ration with DCAB+14); R1 (Ration with DCAB+14 + Cr 3ppm,); R2 (Ration with DCAB 0 + Ca); R3 (Ration with DCAB 0 + Cr 3 ppm + Ca), were allocated in twenty four of 1.5-2 years old Garut rams in a randomized block design. The results showed that Cr supplementation in rations containing different levels of Ca did not affect feed intake, body weight gain, and dry matter digestibility, but reduced the absorption of Cr and Ca of the low Ca diet. Supplementation of Cr had no effect on Cr, Ca, Zn, and Mg status in blood and semen of the rams. Level of Cr intake had negative correlation with Ca absorption and positive correlation with blood Cr levels. There is a positive relationship between level of Ca intake with Ca and Mg absorption and blood Ca and Zn levels. Intake of Cr and Ca was not related to the semen Cr and Ca levels.

Altering physiological conditions and semen acidity by manipulating dietary cation-anion difference and fish oil supplemenation of Garut breed rams

Contribution of Garut breed sheep as protein resources is not optimal yet. Acceleration of population growth such as increasing of female offspring is neccesary. This study was carried out to obtain information regarding the effect of dietary cation-anion difference (PKAR: meq [(Na + K) – (Cl+S)/100 g of dry matter]) and fish oil supplementation on acidity of blood and semen, blood gas and plasma and semen mineral. The dietary treatmens were as follows: RN0= basal ration (PKAR +14) without fish oil, RNI= basal ration (PKAR +14) with 3% fish oil, RB0= base ration (PKAR +40) without fish oil, RBI= base ration (PKAR +40) with 3% fish oil, RA0= acid ration (PKAR -40) without fish oil, and RAI= acid ration (PKAR -40) with 3% fish oil. The rations contained 150 ppm of zinc and were offered to 18 of Garut rams. The result indicated that blood pH, pCO 2 , and pO 2 were not affected by PKAR and fish oil supplementation, but cHCO 3 and cBase were affected (P<0.05). PKAR and fish oil supplementation affected (P<0.01) plasma Mg and S, but did not affect K, Na, Zn and Cl. Semen pH after day 28 of experimental period were highly significant different and that followed PKAR pattern. In conclution, PKAR affected cBase, cHCO 3 , concentration of Mg and S plasma and semen pH of Garut rams. The result suggested that PKAR could be applied to manipulate physiological condition and semen pH. Key Words : PKAR, Physiological Condition, Semen, Garut Ram

The quality of Garut ram liquid semen in Tris egg yolk extender to the sucrose supplementation

The successful program of artificial insemination in sheep is determined by the quality of sperm that are used. Therefore, it is important to maintain the quality of sperm during and after storage in low temperature. The research was conducted to study the influence of sucrose in the Tris-yolk extender in maintaining the quality of Garut ram sperm during preservation in reaction tube for four days at 5°C. Ejaculated sperm was collected once a week for five weeks using artificial vagina from same ram. Semen was divided into four groups of extenders, i.e. Tris egg yolk 20% (TKT), TKT + 0,1% sucrose, TKT + 0,3% sucrose and TKT + 0,5% w/v sucrose. The results showed that the percentage of motility on day four (D-4) of storage in TKT (41.00 ± 2.0%) was lower (P 0.05) from TKT + 0.1% (45.00 ± 3.16%). Whereas the percentage of live cells (%H) on D-4 in TKT, TKT + 0.1%, TKT + 0.3% and TKT + 0.5% were 55.00 ± 2.19%; 57.20 ± 2.79%; 59.20 ± 3.25% and 61.20 ± 3.60%, respectively. Meanwhile, the percentage of membrane integrity (MPU) in TKT (51.80 ± 1.94%) was significantly different (P 0.05) from TKT + 0.1% (54.80 ± 2.86%). In conclusion, the addition of sucrose 0.3% w/v into TKT extender could maintain the quality of Garut ram sperm more efficiently and better than TKT extender. Key Words : Sucrose, Tris Egg Yolk, Preservation, Garut Sheep

Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries)

This study was aimed to examine the quality of chilled-cement of Garut ram which was diluted with Tris extender and various trehalose concentrations. Cement was collected from three mature rams using an artificial vagina once a week. After initial evaluation, the cement was divided into four parts and diluted with Tris extender only (control), Tris extender + 0.20% (TR0.2), Tris extender + 0.40% (TR0.4), and Tris extender + 0.60% (TR0.6) trehalosa respectively. The extended-cement was stored at 5 o C. Parameters of chilled-cement quality examined were percentage of sperm motility, live sperm, and intact plasma membrane (IPM), which were evaluated every day for four days. The results showed that addition of trehalose did not significantly improved the percentage of sperm motility, live sperm, and IPM (P>0.05) of Garut ram sperm stored in refrigerator at 5 o C for 4 days. In conclusion, the addition of trehalose does not enhance the quality of chilled-cement of Garut ram.

Role of various sugars in improving frozen semen quality of Garut ram

Ram spermatozoa are sensitive to extreme changes in temperature during the freeze-thawed process. The present study was conducted to examine the effects of addition of various sugars in Tris extender on sperm cryosurvival of Garut ram. Semen was collected using an artificial vagina from three mature rams once a week. Immediately after initial evaluation, semen was divided into five parts and diluted with Tris extender (control), Tris extender + 0.4% dextrose, Tris extender + 0.4% raffinose, Tris extender + 0.4% trehalose, and Tris extender + 0.4% sucrose, respectively. Semen was loaded in to 0.25 ml mini straw with the concentration of 200 million or 800 million motile spermatozoa per ml. Semen was equilibrated at 5 o C for three hours, then frozen and stored in liquid nitrogen for seven days. Quality of processed-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), intact acrosome cap (IAC), and intact plasma membrane (IPM) were evaluated after dilution, equilibration, and thawing, respectively. Data were analyzed using completely randomized design with five treatments and six replicates. Means were compared significant difference test at 0.05 significant level. Results of this research showed that there was no significantly difference (P>0.05) between treatments for all sperm quality parameters after dilution and equilibration. Mean percentages of post thawing MS, LS, IAC, and IPM for dextrose (54.00; 68.00; 66.60, and 57.83%), raffinose (50.00; 64.33; 61.80, and 61.75%), trehalose (50.83; 65.67; 61.40 and 57.75%), and sucrose (49.00; 66.80; 58.50 and 58.50%) were significantly (P<0.05) higher than control (40.83; 52.67; 54.60, and 49.40%) respectively. In conclusion, addition of 0.4% dextrose, raffinose, trehalose or sucrose in Tris extender are effective in improving frozen semen quality of Garut ram. Key Words: Sugars, Tris extender, Frozen Semen Quality, Garut Ram

Daya Hidup Spermatozoa Epididimis Domba Garut yang Dikriopreservasi Menggunakan Modifikasi Pengencer Tris

Sperm collected from cauda epididymis is a source of male gametes. The purposes of this study was to evaluate an quality of frozen-thawed sperm of garut ram which was collected from cauda epididymis and cryopreserved with modified Tris extender, i.e: Tris extender (control, KT), Tris extender + 60 mM lactose (LS), and Tris extender + 60 mM lactose + 0.05% glutathione (GL). Quality of collected sperm including concentration, motility, live sperm, abnormality, cytoplasmic droplet, intact acrosomal cap (IAC), and intact plasma membrane (IPM) were evaluated. Results showed that mean of sperm concentration, percentages of motility, live sperm, abnormality, cytoplasmic droplet, IAC, and IPM of fresh epididymal sperm were 13,993.33 million/ml, 70.83, 82.83, 10.83, 8.5, 85.83, and 81.33%, respectively. Sperm quality after equilibration for LS and GL were significantly (P

Quality of Garut ram frozen semen in various glycerol concentrations

Semen was collected once a week using artificial vagina from four mature Garut rams. Immediately after initial evaluation, semen was divided into three parts and diluted with tris extender containing 3% (G3), 5% (G5), and 7% (G7) glycerol, respectively, each with the concentration of 100 million motile sperm 0.25 ml-1. Semen was loaded in 0.25 ml mini straws, and equilibrated at 50C for three hours, then frozen and stored in liquid nitrogen container. Results indicated that percentages of post thawing motility and live sperm for G5 (40 and 50.50%) were significantly higher than G3 (32.50 and 45.33%) (P 0.05). Percentages of post thawing intact acrosomal and plasma membrane for G5 (42.67 and 43.17%) were significantly higher than G3 (36.17 and 38.17%) (P 0.05). In conclusion, concentration of 5% glycerol is the optimal dose in maintaining frozen semen quality of Garut rams. Key words : Glycerol concentrations, frozen semen, Garut ram