Weight Gain Research Articles

Effects of high-fat diet on the morphology and function of the thyroid gland and glycolipid metabolism in thyroid clock gene Bmal1 knockout mice

Objective: To investigate the changes in thyroid morphology and function in mice with thyroid-specific knockout of the clock gene Bmal1 under high-fat diet (HFD), and to examine its effects on glycolipid metabolism in mice. Methods: Construct a mouse model with specific knockout of the Bmal1 gene in the thyroid (T-Bmal1-/-) (knockout group, n=10), and use Bmal1flox/flox mice without thyroid peroxidase-cyclization recombination enzyme (T-Bmal1+/+) as the control group (non-knockout group, n=10). The mice were fed until 6 weeks (body weight 20-23 g), and then use the random number table method to evenly divide each group of mice into two subgroups. They were then fed either with normal diet (ND) or (HFD), resulting in four final groups: ND non-knockout group, HFD non-knockout group, ND knockout group, and HFD knockout group, with 5 mice in each group. From the 6th week onwards, the body weights of the mice were measured for 10 consecutive weeks. On the first day of the 14th week, the intraperitoneal glucose tolerance test (IPGTT) was conducted, and on the first day of the 15th week, the intraperitoneal insulin tolerance test (IPITT) was performed. On the first day of the 16th week after the mice were born, blood samples (0.2 ml) were taken from the eyes under anesthesia at 7∶00, 13:00, 19:00, and 1∶00 the next day, and the thyroid, liver, kidneys, spleen, inguinal white adipose tissue, and scapular brown adipose tissue were rapidly removed. Thyroid tissue morphology, thyroid function indexes, thyroid clock genes and thyroid hormone synthesis and secretion related genes expression, body weight, IPGTT and IPITT area under the curve (AUC) and lipid levels were detected and compared among all groups. Results: There was no significant difference in the number of thyroid follicles, the area of thyroid follicles and the height of thyroid follicle cavity between ND knockout group and ND non-knockout group (all P>0.05), but the height of thyroid follicle cavity in HFD knockout group was lower than that in HFD knockout group [(25.8±1.6) vs (54.4±9.5) μm, P=0.002]. The level of serum T4 in knockout group after ND or HFD feeding was higher than that in non-knockout group (all P<0.05). There was no significant difference in the mRNA expression level of sodium iodine transporter between ND knockout group and ND non-knockout group (P=0.550), but the mRNA expression level of sodium iodine transporter in HFD knockout group was higher than that in HFD non-knockout group [0.67±0.27 vs 0.20±0.09, P=0.006].There was no significant difference in weight gain and groin white fat weight between ND knockout group and ND non-knockout group (both P<0.05), but weight gain and groin white fat tissue weight in HFD knockout group were lower than those in HFD knockout group (both P<0.05). The IPGTT results showed no statistically significant difference in AUC between the ND knockout and ND non-knockout groups (P=0.226), but the HFD knockout group had a smaller AUC than the HFD non-knockout group (P=0.008). The IPITT revealed that the AUC of the ND knockout group was smaller than that of the ND non-knockout group (P=0.047). Lipid profiles were similar between the ND knockout and ND non-knockout groups (all P>0.05), but the HFD knockout group had lower levels of total cholesterol and high-density lipoprotein cholesterol than the HFD non-knockout group (both P<0.05). Conclusion: Thyroid-specific knockout of the clock gene Bmal1 can affect thyroid hormone levels and glycolipid metabolism in mice, with more pronounced effects after HFD feeding.

Physical Activity and Weight Gain During Pregnancy: Is This Association Modified by Sleep?

ABSTRACT Objective To evaluate the cross-sectional association of physical activity during pregnancy with gestational weight gain and verify if associations are modified by levels of sleep duration and efficiency. Methods: Mothers who were participants of the 2015 Pelotas (Brazil) Birth Cohort study were analyzed. Physical activity (PA) and sleep were measured using the nondominant wrist accelerometer model wGT3X-BT (ActiGraph, Pensacola, FL). Moderate-to-vigorous activities were measured in bouts of 5 minutes (MVPA 5-min bout). Activities were characterized as sustained and overall physical activity was expressed in mg, and MVPA (non-bouted) activities were characterized as not sustained. Sleep was presented as minute bouts, sleep time window, total sleep time, and sleep efficiency. Gestational weight gain (GWG) was calculated by a woman’s weight at the end of gestation minus the pre-pregnancy weight. Linear regression models were used to evaluate the association between PA and GWG. Stratification by sleep characteristics in tertiles was performed. Results: The analytical sample included 1,938 women with information for PA, sleep, and GWG. The mean GWG was 12,030 g (SD = 6658.8). After adjustments, for every 15 minutes of sustained physical activity (MVPA 5-min bout), the GWG decreased by 591.3 g [β= −591.3 (95%CI: −1,147.1; −35.5). A statistically significant association was observed between 15 minutes of sustained physical activity (MVPA 5-min bout) and GWG in the second and third tertiles of healthier sleep efficiency. Conclusion: MVPA with 5-min bouts is associated with lower GWG. The GWG reduction was greater in the higher sleep efficiency strata.

Relationship between the ratio of increase in lean tissue to body weight gain and energy required to gain body weight in growing rats.

Although the energy stored in the lean tissue (LT) and adipose tissue (AT) is well known, the energy required to synthesize these tissues is obscure. Theoretically, the energy at the point at which ΔLT/Δ body weight (BW) reaches 100% on a regression line, which indicates the relationship between ΔLT/ΔBW and the energy required for BW gain, is considered to be the energy expended to synthesize LT. Therefore, we investigated this relationship in rats. Rats were fed diets with different ratios of protein, fat, and carbohydrates because their ΔLT/ΔBW values were expected to be different. Six-week-old male Sprague-Dawley rats had ad libitum access to normal (N, n = 6), high-fat (HF, n = 7), or high-protein (HP, n = 8) diets for 4 weeks. The ΔLT/ΔBW was 0.77 in the N group, 0.70 in the HF, and 0.87 in the HP groups, respectively. The average energy required to gain BW was 8.8 kJ/g in the N group, 7.0 kJ/g in the HF group, and 11.3 kJ/g in the HP group. We observed a positive correlation between ΔLT/ΔBW and energy required for BW gain. The regression line demonstrated that the energy expended to synthesize LT was 13.9 kJ/g and AT was -7.9 kJ/g. Therefore, combined with the energy stored in LT, the energy required to accumulate LT is approximately 19 kJ/g, whereas the energy to accumulate AT could not be elucidated.

Maternal swimming with overload allied to postnatal high-fat, high-sugar diet induce subtle impairment on rat offspring’s ovarian redox homeostasis

Context The Developmental Origins of Health and Disease (DOHaD) concept suggests that early-life interventions significantly influence the long-term health outcomes of offspring. Emerging evidence supports that maternal physical exercise and balanced nutrition can positively impact the health of the next generation. Aims This study investigated the effects of maternal swimming combined with postnatal high-fat, high-sugar (HFHS) diet on the ovarian health of adult female Wistar rat offspring. Methods Adult female Wistar rats performed swimming exercise in a controlled temperature environment (32°C, 2% bodyweight overload adjusted daily) during 4 weeks, starting 1 week prior mating. The female offspring received a control or HFHS diet from postnatal day (PND) 21 to PND 90. We analyzed offspring’s body weight, ovarian histomorphology, redox status, and associated molecular pathways 5′ adenosine monophosphate-activated protein kinase (AMPKα), forkhead box O3 (FoxO3), and mitofusin 1 (Mfn-1). Key results Our findings reveal that maternal swimming exerted an effect on offspring body weight gain, delaying it. Individually, maternal exercise reduced superoxide dismutase (SOD) activity and mitofusin-1 levels, while the postnatal HFHS diet alone decreased both SOD and glutathione peroxidase (GPx) activities and increased the pFOXO3a/FOXO3a ratio in the ovaries. Conclusions We showed that combination of maternal swimming with a 2% overload and a postnatal HFHS diet can negatively affect the ovarian redox balance in offspring. Implications Prenatal and postnatal lifestyle might affect reproductive function in females.

Impact of anxiety symptoms on dialysis adherence and complication rates: A longitudinal observational study

BACKGROUND Anxiety is a common psychological comorbidity in patients undergoing dialysis, yet its impact on treatment adherence and complication rates remains understudied. We designed a longitudinal observational study to investigate these relationships, based on the hypothesis that higher anxiety symptoms would be associated with increased complication rates and negatively associated with adherence to the dialysis regimen. AIM To investigate the relationship between anxiety symptoms, dialysis adherence, and complication rates in patients undergoing dialysis over a 24-month period. METHODS This observational study analyzed data from 250 adult patients who underwent hemodialysis or peritoneal dialysis at three Affiliated Hospitals of Youjiang Medical University for Nationalities over a period of 24 months. Anxiety symptoms were assessed using the Hospital Anxiety and Depression Scale-Anxiety subscale at baseline and every 6 months. Dialysis adherence was evaluated through attendance records, interdialytic weight gain, and patient-reported medication adherence. We recorded complications (infections, cardiovascular events, and hospitalizations) and used mixed-effects models and survival analyses to infer associations between anxiety symptoms, adherence measures, and complication rates. RESULTS Higher anxiety symptoms were significantly associated with poorer dialysis adherence, including increased missed sessions [incidence rate ratio = 1.32, 95% confidence interval (CI): 1.18-1.47, P &lt; 0.001], greater interdialytic weight gain (β = 0.24, 95%CI: 0.15-0.33, P &lt; 0.001), and lower medication adherence (odds ratio = 0.85, 95%CI: 0.78-0.93, P &lt; 0.001). Patients with clinically significant anxiety (Hospital Anxiety and Depression Scale-Anxiety subscale ≥ 8) had a higher risk of complications [hazard ratio (HR) = 1.68, 95%CI: 1.32-2.14, P &lt; 0.001], particularly infections (HR = 1.89, 95%CI: 1.41-2.53, P &lt; 0.001) and cardiovascular events (HR = 1.57, 95%CI: 1.18-2.09, P = 0.002). The relationship between anxiety and complications was partially mediated by adherence measures. CONCLUSION Anxiety symptoms in patients undergoing dialysis are associated with poorer treatment adherence and increased complication rates. Regular screening and targeted interventions to address symptoms may improve adherence and clinical outcomes.

Pediococcus acidilactici Y01 reduces HFD-induced obesity via altering gut microbiota and metabolomic profiles and modulating adipose tissue macrophage M1/M2 polarization.

Obesity-related metabolic syndrome is intimately associated with infiltrated adipose tissue macrophages (ATMs), gut microbiota, and metabolic disorders. Pediococcus acidilactici holds the potential to mitigate obesity; however, there exist strain-specific functionalities and diverse mechanisms, which deserve extensive exploration. This study aims to explore the potential of P. acidilactici Y01, isolated from traditional sour whey, in alleviating HFD-induced metabolic syndrome in mice and elucidating its underlying mechanism. The results showed that P. acidilactici Y01 could inhibit the increase of body weight gain, the deposition of fat, lipid disorders and chronic low-grade inflammation, improve glucose tolerance and insulin resistance, and could reduce adipose tissue inflammation by decreasing M1-type ATMs and increasing M2-type ATMs. Meanwhile, P. acidilactici Y01 significantly increased the abundance of potentially beneficial intestinal bacteria, such as Akkermansia, Alistipes, Bifidobacterium, Lachnospiraceae_NK4A136_group, Lactobacillus, norank_f__Muribaculaceae, and Parabacteroides, and partially restored the levels of metabolites, such as phosphatidylcholines, glycerophosphocholines, sphingolipids and unsaturated fatty acids. The fecal microbiota transplantation experiment demonstrated that P. acidilactici Y01 ameliorated obesity-related metabolic syndrome by modulating the polarization of M1/M2 ATMs mediated by gut microbiota. Overall, as a dietary supplement, P. acidilactici Y01 has good potential in the prevention and treatment of obesity.

Impact of weight change on kidney transplantation outcomes: A systematic review and meta-analysis.

Kidney transplant recipients frequently experience a wide range of metabolic complications, including weight changes, which significantly impact patient outcomes and graft function, yet the relationship between weight gain and transplant outcomes remains poorly understood. This systematic review and meta-analysis aimed to synthesise existing evidence on the influence of weight gain on patient and graft outcomes following kidney transplantation to enhance clinical practice and optimise post-transplant care strategies. A literature search was conducted across databases such as PubMed and Scopus for peer-reviewed studies published up to 8 August 2024. We included adult kidney transplant recipients (ages 18 years and older) with substantial and clinically relevant post-transplant weight gain and a control group without such changes, focusing on outcomes including all-cause mortality, graft survival, cardiovascular events and acute rejection. The pooled analysis, which included data from 11 studies, indicated no significant association between post-transplant weight gain and the risk of all-cause mortality (hazard ratio [HR] 1.21, 95% confidence interval [CI] 0.69 to 2.10, p = 0.51; I2 = 28%), cardiovascular events (HR 0.93, 95% CI 0.43 to 2.01, p = 0.85; I2 = 32%) or acute rejection (HR 1.13, 95% CI 0.76 to 1.68, p = 0.55; I2 = 9%). However, weight gain was significantly associated with an increased risk of graft failure (HR 1.58, 95% CI 1.22 to 2.05, p < 0.001; I2 = 0%). Substantial and clinically relevant weight gain after kidney transplant was associated with a higher risk of graft failure. Within the timeframes of study observation, risks of all-cause mortality, cardiovascular events or acute rejection were not increased by weight gain in kidney transplant recipients.

Intrapatient Changes in CT-Based Body Composition After Initiation of Semaglutide (Glucagon-Like Peptide-1 Receptor Agonist) Therapy.

BACKGROUND. The long-acting glucagon-like peptide-1 receptor agonist semaglutide is used to treat type 2 diabetes or obesity in adults. Clinical trials have observed associations of semaglutide with weight loss, improved control of diabetes, and cardiovascular risk reduction. OBJECTIVE. The purpose of this study was to evaluate intrapatient changes in body composition after initiation of semaglutide therapy by applying an automated suite of CT-based artificial intelligence (AI) body composition tools. METHODS. This retrospective study included adult patients who were receiving semaglutide treatment and who, between January 2016 and November 2023, underwent abdominopelvic CT within both 5 years before and 5 years after initiation of semaglutide. An automated suite of previously validated CT-based AI body composition tools was applied to scans obtained before semaglutide initiation (hereafter, presemaglutide scans) and scans obtained after semaglutide initiation (hereafter, postsemaglutide scans) to quantify visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) area, skeletal muscle area and attenuation, intermuscular adipose tissue (IMAT) area, liver volume and attenuation, and trabecular bone mineral density (BMD). Patients with weight loss of 5 kg or more and those with weight gain of 5 kg or more between the scans were compared. RESULTS. The study included 241 patients (151 women and 90 men; mean age, 60.4 ± 12.4 [SD] years). In the weight-loss group (n = 67), the postsemaglutide scan, compared with the presemaglutide scan, showed a decrease in VAT area (309.4 vs 341.1 cm2, p < .001), SAT area (371.4 vs 410.7 cm2, p < .001), muscle area (179.2 vs 193.0, p < 0.001), and liver volume (2379.0 vs 2578 HU, p = .009) and an increase in liver attenuation (74.5 vs 67.6 HU, p = .03). In the weight-gain group (n = 48), the postsemaglutide scan, compared with the presemaglutide scan, showed an increase in VAT area (334.0 vs 312.8, p = .002), SAT area (485.8 vs 448.8 cm2, p = .01), and IMAT area (48.4 vs 37.6, p = .009) and a decrease in muscle attenuation (5.9 vs 13.1, p < .001). Other comparisons were not statistically significant (p > .05). CONCLUSION. Patients using semaglutide who lost versus gained weight showed distinct patterns of changes in CT-based body composition measures. Those with weight loss had overall favorable shifts in measures related to cardiometabolic risk. A decrease in muscle attenuation in those with weight gain is consistent with decreased muscle quality. CLINICAL IMPACT. Among patients using semaglutide, automated CT-based AI tools provide biomarkers of changes in body composition beyond those that are evident by standard clinical measures.

Guanidinoacetic acid supplementation and stocking density effects on broiler performance: behavior, biochemistry, immunity, and small intestinal histomorphology

BackgroundRearing poultry under stressful high stocking density (HSD) conditions is a common commercial practice to increase profitability, despite its negative effects on broiler physiology and welfare. Many feed additives are used to alleviate the negative impact of such practices. This study investigated the ameliorative effects of guanidinoacetic acid (GAA) on growth performance, ingestive behavior, immune response, antioxidant status, stress indicators, and intestinal histomorphometry of broilers subjected to HSD. A total of 364 male broilers were randomly allocated into four treatments with 7 replicates each in a 2 × 2 factorial arrangement: two stocking densities (SD) (10 and 16 birds/m2) and two GAA levels (0 and 0.6 g/kg feed).ResultsBody weight, weight gain, feed intake, feed conversion ratio, production efficiency factor, dressing yield, and ingestive behavior were negatively affected by HSD, whereas the mortality rate was unaffected (P > 0.05). GAA improved the overall growth performance and dressing percentage (P < 0.05). In the HSD group, the immune response decreased at d 21 (P < 0.05). Creatine kinase, glutathione peroxidase (GPX), superoxide dismutase, catalase, triglycerides, and villus length and width (ileum) were reduced, whereas corticosterone (CORT) was increased (P < 0.05). Moreover, GAA increased the hemagglutination-inhibition titer at 21 days and the levels of lactate dehydrogenase, GPX, and catalase and decreased the levels of creatinine, alanine aminotransferase, nitrite, triglycerides, and CORT (P < 0.05). SD and GAA did not affect malondialdehyde or other biochemical parameters (P > 0.05).ConclusionsDietary GAA supplementation can improve productivity and antioxidant status and reduce stress in broilers reared in a HSD environment.

BMI trajectory of 8,155,894 Japanese adults from exhaustive health checkup data: the contributions of age-related changes in height and weight.

This study aimed to clarify the trajectory of BMI alongside age-related changes in height and weight among Japanese adults. Data from annual health checkups between 2015 and 2020 by the Japan Health Insurance Association were retrospectively analyzed. A total of 4,777,891 men and 3,378,003 women (age 35-69 years) were stratified into 14 subgroups based on sex and 5-year age categories. We used linear mixed-effects model to estimate values for each outcome, with six-time points (2015-2020) as the independent variable. Mean BMI changes were positive across all subgroups, indicating a trend of increasing BMI (men, 0.02 to 0.14 kg/m2/year; women, 0.05 to 0.16 kg/m2/year). In younger subgroups, the changed were relatively large, with the weight transitions mirroring those of BMI. However, the mean changes were negative (men, -0.06 kg/year; women, -0.01 kg/year) in the oldest subgroups. Height reduction increased with age across subgroups (men, -0.14 to -0.03 cm/year; women, -0.18 to -0.01 cm/year). In conclusion, BMI tended to increase with age in both sexes across all age groups of Japanese adults. The increase in BMI appeared to be influenced by weight gain in young to middle age, whereas height reduction influenced increased BMI in older age groups.

Dietary inclusion of black soldier fly (Hermetia illucens) larvae meal, with exogenous protease supplementation, in practical diets for striped catfish (Pangasius hypophthalmus, Sauvage 1878).

Fishmeal (FM) is a key component of commercial fish feeds, but due its unsustainable supply, the search for quality alternatives of FM has become a significant area of investigation worldwide. The insect-based proteins such as black soldier fly larvae (BSFL) are being recognized as an alternative ingredient. However, anti-nutritional factors in these alternatives may negatively affect nutrient utilization in fish. Incorporating exogenous protease enzymes as feed additives could be a promising way to improve the digestibility of these alternative ingredients. Therefore, this study aimed to evaluate the impact of dietary inclusion of BSFL meal, combined with protease supplementation, on various parameters of striped catfish (Pangasius hypophthalmus). Five diets were formulated: a control diet (FM-based), two diets with 30% and 60% BSFL inclusion (BSF30 and BSF60), and two diets with the same inclusion levels plus the incorporation of exogenous protease (BSF30P and BSF60P). One hundred fifty fish (8.26±0.02 g) were arbitrarily allocated to five groups, each with three replicates. The fish were provided with their respective diets twice per day for 60 days. In comparison to the control, fish-fed diets supplemented with protease demonstrated statistically significant lower levels of feed conversion ratio and higher growth performance regarding the final body weight and weight gain. Lipase activity in the BSF60P group was notably greater than in the control group. Protease supplementation significantly enhanced the apparent digestibility coefficient of protein, intestinal protease activity, and crude protein content in the whole body. Most hematological and biochemical parameters remained unaffected except for substantially lower triglyceride and cholesterol levels in the highest BSFL inclusion groups. These groups also showed a reduction in crude fat contents. While glutathione peroxidase and malondialdehyde levels did not change significantly from the control, the liver tissues of fish fed BSF60P diets showed considerably higher levels of antioxidant enzymes such as catalase and superoxide dismutase. The findings suggest that including 60% of a BSFL based diet, along with exogenous protease supplementation, is feasible without compromising the growth performance and health of striped catfish.

Long-term impact of maternal pre-pregnancy BMI on the risk of overweight/obesity in preschool children: mediation by infant birth weight and BMI trajectory group from 0 to 2 years

BackgroundObesity is a complex multisystem disease that affects almost every organ system. Studying the early determinants of obesity and implementing targeted interventions have positive implications for promoting long-term health. Therefore, this study was to evaluate the impact of maternal pre-pregnancy overweight or obesity, weight gain rate (WGR) during the second and third trimesters, infant birth weight (IBW) and BMI trajectory group (BMI-TG) from 0 to 2 years on overweight or obesity in preschool children.MethodsA retrospective cohort analysis of data obtained from a hospital was performed using chain-mediated effects to analyze the direct and indirect effects of prepregnancy body mass index (ppBMI)on preschool children’s body mass index (pcBMI), and mediated by three variables: WGR, IBW and BMI-TG.ResultsWe found that the effect of ppBMI and pcBMI was significantly mediated by IBW and BMI-TG. After adjusting for confounding factors, the total effect of ppBMI on pcBMI was estimated to be 0.1965 (bootstrap 95% CI: 0.1154–0.2783), the direct effect was 0.1562 (bootstrap 95% CI: 0.0750–0.2362), and the indirect effect was 0.0403 (bootstrap 95% CI: 0.0219 ~ 0.0651). The mediating effect accounted for 20.5% of the variance, indicating that 20.5% of the impact of ppBMI on pcBMI was mediated by two mediating factors: IBW and the BMI-TG.ConclusionsMaternal overweight/obesity before pregnancy had a significant impact on overweight/obesity in preschool children and was mediated by two main factors: IBW and the BMI-TG.

Early milk cereal drink consumption correlated with rapid weight gain but not with subsequent adiposity.

The role of infant nutrition in rapid weight gain (RWG) is not well understood. This study investigated associations between milk cereal drinks, early RWG, and later body mass index (BMI) and waist-to-height ratio (WHtR). In total, 1333 children, from three communities in Western Sweden constituted the combined IDEFICS-I. Family cohort. Data were obtained retrospectively from the National Medical Birth Register, child health centres and school health services. Anthropometric data and parental reported food habits were collected in the 2007-2008 IDEFICS baseline survey. Follow-up data for 656 children were collected in the 2013-2014 I. Family survey. Linear and logistic regressions were used. At baseline, 820 (62%) of the 658 boys and 675 girls, median age 6.0 years, interquartile range 4.0-7.5 years, consumed milk cereal drinks and 229 (18%) had early RWG. Milk cereal drinks were associated with early RWG without effects on later BMI or WHtR status 6 years later. Early RWG was predictive of higher anthropometric measures later in childhood, particularly abdominal adiposity. Despite early-life correlations between RWG and milk cereal drinks, this study could not demonstrate a long-term detrimental association between milk cereal drinks and BMI or WHtR.

Efficacy and safety of switching to insulin glargine 300 U/mL in people with type 2 diabetes uncontrolled on basal insulin in China: A post hoc subpopulation analysis of the INITIATION study.

To evaluate the efficacy and safety of insulin glargine 300 U/mL (Gla-300) in people with uncontrolled type 2 diabetes (T2D) switching from another basal insulin (BI). INITIATION was an interventional, single-arm, phase IV study conducted in China. In this post hoc subpopulation analysis, the efficacy and safety of switching to Gla-300 was investigated in individuals with uncontrolled T2D (HbA1c 7.5%-11.0% [58-97 mmol/mol]) with previous BI. The primary endpoint was HbA1c change at week 24. Other measures of glycaemia, hypoglycaemia, insulin dose and weight change were assessed. Three hundred and two participants switched to Gla-300 from another BI, including 232 from insulin glargine 100 U/mL (Gla-100) and 55 from insulin degludec (IDeg). At week 24, the mean ± standard error (SE) HbA1c change from baseline was -0.87% ± 0.06% (-9.5 ± 0.7 mmol/mol; p <0.001). Significant reductions in fasting plasma glucose (least-squares mean [LSM] change -1.13 mmol/L) and fasting self-measured blood glucose (LSM change -1.36 mmol/L) were also observed (both p <0.001). The mean daily BI dose increased from 18.86 U (0.27 U/kg) at baseline to 28.83 U (0.41 U/kg) at week 24. During the 24-week treatment period, the incidence of any hypoglycaemia was 43.8% for all hypoglycaemia and 15.1% for nocturnal hypoglycaemia; the incidence of severe hypoglycaemia was low (0.7%). Minimal body weight change was documented. Gla-300 improved glycaemic control with a relatively low hypoglycaemia risk and minimal weight gain in Chinese people with T2D uncontrolled on previous BI.

Effect of Dried and Fermented Food Leftover on Feed Intake, Growth Performances and Digestibility of Sheep

Background: The Leftover food as a source of animal feed offers a sustainable solution to minimize waste, conserve resources and provide nutritious feed for small ruminants. The experiment was conducted at Mekelle University’s Small Ruminant Research Farm to assess the impact of dried and fermented food leftover on the growth performance and digestibility of nutrients in sheep. Methods: Twenty-one intact Highland lambs with an average weight of 15.17±1.79 kg were utilized in a randomized complete block design, with three treatments replicated seven times. The treatments included: Recommended concentrate mixture supplementation (T1), 71% replacement of the concentrate in T1 with dried food leftover (T2) and 71% replacement of the concentrate in T1 with fermented food leftover (T3). Food leftovers were collected and utilized in both dried and fermented forms. Additionally, adlibitum grass hay and water were provided throughout the trial period. Data analysis was conducted using ANOVA with the general linear model (GLM) procedures of SAS statistical software. Result: Results indicated that dried food leftover had a higher dry matter percentage when compared to the fermented counterpart, though they exhibited similar crude protein, energy and fiber contents. Dry matter intake, body weight gain and feed conversion efficiency were significantly higher (P less than 0.05) for T3 compared to T1 and T2. However, dry matter and nutrient digestibility were not significantly affected by the diets (P greater than 0.05). The findings suggest that utilizing food leftover in fermented form could be more beneficial for feed utilization and animal productivity.

Establishment and evaluation of a carotid artery dissection model in rats

BackgroundGiven the lack of models for carotid artery dissections (CAD), we aim to investigate effects of beta-aminopropionitrile (BAPN) combined with physical damage on the arterial walls of rats, and to establish a high-incidence and low-mortality CAD model.MethodsSixteen SPF SD rats (3-week-old) were divided into two groups. Group B was given 0.25% BAPN solution and group W was given water. Then we established an animal model of CAD by carotid artery torsion. One of the two carotid arteries in each rat was randomly selected for torsion. We got four groups of blood vessels following above-mentioned methods: BAPN plus torsion group (group Bt), BAPN plus non-torsion group (group Bn), water plus torsion group (group Wt), and water plus non-torsion group (group Wn). The hematoxylin and eosin (HE) staining and Verhoeff’s Van Gieson (EVG) staining were performed to observe structures of arteries. Immunofluorescence staining was used to detect structural proteins in vessels. We used triphenyltetrazolium chloride (TTC) staining and neurological function assessment to detect the infarct area of brain and neurological deficits in rats with carotid dissection to verify the validity of the rat model.ResultsBAPN treatment significantly affected the weight gain of rats, but had little effect on survival during the first 5 weeks. The group Bt had the highest incidence of CAD among all groups (p = 0.014). HE staining of carotid artery tissue sections showed that the vascular walls were the thickest in group Bt (p &amp;lt; 0.001). EVG staining showed the arrangement of elastic fibers was the most irregular in group Bt. Immunofluorescence staining revealed that the expression of a-SMA and SM22a were decreased remarkably in group Bt (p &amp;lt; 0.001). Both motor and sensory deficits were more severe in CAD group than control group (p = 0.0004; p = 0.0036). The relative infarction volumes of CAD group rats were significantly larger than control group (p &amp;lt; 0.001).ConclusionThe animal model of CAD can be feasible to establish by mechanical torsion combined with BAPN free drinking. With this method, the animal mortality was low and the model formation rate was high. This model will enable further studies on CAD.

Leveraging Lactobacillus plantarum probiotics to mitigate diarrhea and Salmonella infections in broiler chickens

Anaerobic bacteria, such as Lactobacillus plantarum (LP), are known to play a significant role in maintaining gut health and protecting against enteric pathogens in animals. The present study aimed to develop a safe, affordable, and eco-friendly method for producing LP-based probiotics and evaluate their efficacy in mitigating Salmonella-induced diarrhea in broiler chickens. The study employed three different culture media (MRS, TSB, and Baird Parker) to grow LP, which was then dried using a spray-drying technique to produce a stable probiotic formulation. When administered to broiler chickens, the LP probiotic derived from the MRS medium significantly improved body weight gain (4.147-fold increase over 4 weeks) compared to the other two culture conditions. Importantly, the LP probiotic treatment could substantially reduce the diarrhea index in broilers, with up to an 86.45% improvement in Salmonella-induced enteric infections. The beneficial effects were attributed to the ability of LP to modulate the gut microbiome, enhance the integrity of the intestinal mucosa, and mitigate the pathogenic effects of Salmonella. These findings demonstrate the potential of anaerobic Lactobacillus plantarum as a safe and effective probiotic intervention for controlling enteric diseases and improving production outcomes in poultry farming. The developed method provides a sustainable approach to harness the beneficial properties of this anaerobic bacterium for animal health and welfare.Graphical abstract

The hidden impact of GLP-1 receptor agonists on endometrial receptivity and implantation.

Increasing infertility rates represent a growing medical challenge in modern societies resulting from a complex interplay of sociocultural trends, lifestyle factors, exposure to environmental toxins, and underlying health problems. Women's fertility is particularly vulnerable to these shifts. The obesogenic lifestyle not only accelerates weight gain, but also disrupts ovulation driving the rise in infertility. Among several medications used for treating obesity and type 2 diabetes, glucagon-like peptide-1 receptor agonists (GLP-1RAs) show promising improvement in female fertility most likely by stimulating ovulation. However, the effects of GLP-1RAs on the endometrium remain unclear. Further studies are needed to investigate the impact of GLP-1RAs on endometrial receptivity and embryo implantation and early development. The aim of this study is to address the knowledge gap regarding the effects of GLP-1RAs on human reproduction, with special focus on the endometrium. Understanding these mechanisms may help to develop new strategies for improving fertility treatment, reduce implantation failureand address potential safety concerns regarding teratogenicity and adverse developmental outcomes for children born to women conceiving during or soon after GLP-1RA treatment.

Dietary Supplementation of Turmeric Rhizome Promotes the Growth Performance, Serum Lipid Profile and Intestinal Microflora of Broilers

The injudicious use of antibiotics as growth promoters in poultry rations has caused great concern around the world. Scientists are currently trying to replace broadspectrum antibiotics in animal diets with alternative natural feed additives. The current study aimed to investigate the dietary effect of turmeric rhizome powder (TP) on the growth performance, serum lipid profiles, and intestinal microflora of broiler chickens. A total of 75 Cobb-15 broiler chicks (15-day-old) were randomly distributed into five groups offered with five experimental rations designated as T0 (control diet, without TP), T1 (1 g TP/kg feed), T2 (2.5 g TP/kg feed), T3 (5 g TP/kg feed), and T4 (7.5 g TP/kg feed). Parameters such as body weight gain, total feed intake, and feed conversion ratio (FCR) were used to assess the response of broiler chickens to feed turmeric powder. Supplementation with 5 g TP/kg feed (T3) resulted in the highest increase in body weight with a relatively lower FCR. Serum levels of cholesterol, triacylglycerol, and LDL-cholesterol decreased significantly when supplemented with 5 g TP/kg feed. The bacteriological investigation revealed that a turmeric-supplemented diet significantly lowered the total Salmonella spp. count. The antioxidant property of turmeric rhizome may alleviate stress in broiler chickens. Taken together, these findings indicate that supplementing broiler ration with 5 g TP/kg feed improved growth performance, lipid profiles, and intestinal microflora of broiler chickens, suggesting that turmeric rhizome can be a potential alternative feed additive to synthetic antibiotics for safe and organic poultry meat production. J. of Sci. and Tech. Res. 6(1): 117-124, 2024

Effects of Isalo scorpion cytotoxic peptide on growth, immune performance, and intestinal flora of yellow-feathered broilers

IntroductionThis study aimed to investigate the effects of Isalo scorpion cytotoxic peptide (IsCT) on the growth performance, immune function, and gut microbiota of yellow-feathered broilers.MethodsThe experiment involved supplementing a corn-soybean meal-based diet with various dietary levels of IsCT. The experiment adopted a completely randomized design. A total of 240 one-day-old yellow-feathered broilers were randomly divided into six groups: a control group (CON), a chlortetracycline group (CTC), and four groups receiving a basal diet with 50, 100, 150, or 200 mg/ kg IsCT (IsCT50, IsCT100, IsCT150, and IsCT200). The trial period lasted 60 days.ResultsThe results showed that the average final body weight and average daily weight gain of the IsCT150 and IsCT200 groups were significantly higher than those of the CON group (p &amp;lt; 0.05). Compared to the CTC group, the average daily feed intake in group III was significantly lower (p &amp;lt; 0.05). The feed conversion ratios of the IsCT150 and IsCT200 groups were significantly lower than those of the IsCT50 and CON groups (p &amp;lt; 0.05). Albumin levels in the CTC and IsCT150 groups were significantly higher than those in the CON, IsCT100, and IsCT50 groups. Alanine transaminase levels in the IsCT150 group were significantly lower than those in the IsCT50, IsCT200, CON, and CTC groups (p &amp;lt; 0.05), but there was no significant difference compared with the IsCT100 group (p &amp;lt; 0.05). IgA levels in the IsCT150, IsCT200, and CTC groups were significantly higher than those in the IsCT50 group (p &amp;lt; 0.05). IgG levels in the CTC, IsCT100, IsCT150, and IsCT200 groups were significantly higher than those in the IsCT50 and CON groups (p &amp;lt; 0.05). 16S rRNA sequencing indicated significant differences in the caecal microbiota between the IsCT and CON groups (p &amp;lt; 0.05). The abundance of Bacteroidetes increased, whereas that of Firmicutes decreased in the IsCT groups; however, the difference was not significant (p &amp;lt; 0.05). The relative abundances of Actinobacteriota and Patescibacteria were significantly different in the IsCT100 group (p &amp;lt; 0.05).DiscussionIn conclusion, supplementing the basal diet with IsCT improved growth performance, immune function, and gut microbiota of yellow-feathered broilers at an optimal supplementation level of 150 mg/kg.