Fusarium Diseases Research Articles

Harnessing RNA interference for the control of Fusarium species: A critical review.

Fusarium fungi are a pervasive threat to global agricultural productivity. They cause a spectrum of plant diseases that result in significant yield losses and threaten food safety by producing mycotoxins that are harmful to human and animal health. In recent years, the exploitation of the RNA interference (RNAi) mechanism has emerged as a promising avenue for the control of Fusarium-induced diseases, providing both a mechanistic understanding of Fusarium gene function and a potential strategy for environmentally sustainable disease management. However, despite significant progress in elucidating the presence and function of the RNAi pathway in different Fusarium species, a comprehensive understanding of its individual protein components and underlying silencing mechanisms remains elusive. Accordingly, while a considerable number of RNAi-based approaches to Fusarium control have been developed and many reports of RNAi applications in Fusarium control under laboratory conditions have been published, the applicability of this knowledge in agronomic settings remains an open question, and few convincing data on RNAi-based disease control under field conditions have been published. This review aims to consolidate the current knowledge on the role of RNAi in Fusarium disease control by evaluating current research and highlighting important avenues for future investigation.

Measurement of the effectiveness of Clonostachys rosea in reducing Fusarium biomass on wheat straw.

The survival and growth of plant pathogens on crop residues are key factors facilitating the dynamics of crop diseases. Spores (e.g., perithecia, and chlamydospores) and mycelium of pathogenic fungi overwinter on harvest residues, such as straw, and serve as initial inoculum infecting crops in the next growing season. Therefore, targeting overwintering fungi is essential to attaining effective disease control. Beneficial microorganisms offer advantages in controlling pathogens through their ability to colonize and exploit different environmental niches. In this study, we applied qPCR assays to explore the biocontrol performance of locally isolated strains of Clonostachys against various Fusarium pathogens. We proved that prior colonization of wheat straw by Fusarium spp. can be effectively reduced by Clonostachys rosea. We demonstrated that the efficiency of C. rosea to reduce Fusarium inoculum appears to remain at a similar level for most studied strains regardless of the target pathogen and the level of colonization of substrates by pathogens. Efficient performance of local C. rosea strains identifies possible targets for future strategies to control Fusarium diseases in cereals. Our study also highlights the challenge in sequence-based determination of C. rosea, which is crucial for the efficient selection of beneficial strains for biocontrol purposes.

Эффективность протравливания семян тритикале препаратами защитного действия в Приамурье

Abstract. The purpose is to establish a productive seed disinfectant for spring triticale varieties with a protective and stimulating effect. During field experiments methods were used that tested pesticides and determined the economic efficiency of using research results in agriculture. The relevance and national and economic significance of the development is determined by farmers’ demand in the extreme conditions of the Far East to increase the efficiency of treating triticale seeds, identifying protective agrochemicals against a complex of diseases that help to improve biological and economic indicators when cultivating triticale. The scientific novelty of research is associated with the establishment of effective agrochemicals to protect seedlings from pathogens of the genus Fusarium and reduce grain losses of spring triticale varieties in years of different meteorological conditions. Results. Biological effectiveness of agrochemicals against fungal diseases Fusarium spp., Alternaria spp. and B. sorokiniana was revealed in laboratory conditions. High biological effectiveness was reveled for “Maxim”, average – for “Kinto Duo” and low – for “Inshur Perform”. The greatest effect from treating seeds with agrochemicals was in Ukro variety, followed by Karmen and Yarilo. Significantly high effectiveness of agrochemicals “Maxim” and “Kinto Duo” against strains of fungi from the genus Fusarium in field conditions was revealed on seedlings of Ukro and Yarilo varieties. The prevalence of diseases in triticale seedlings depended by 24.1 % on year conditions, by 7.7 % on genotype and by 42.3 % on agrochemicals. Fungicidal seed disinfectants “Kinto Duo” and “Maxim” (2 l/t) confirmed the effect declared by the manufacturers and statistically significantly surpassed the seed disinfectant “Inshur Perform” (0.5 l/t) in biological effectiveness and positive effect on grain yield of triticale varieties. They contributed to obtain grain yields that significantly exceeded the control level by 0.3 and 0.33 t/ha. Their conditionally net income in comparison with the option without a fungicides reached +3298.8 and +2314.1 rubles/ha, respectively.

Efficiency of pre-sowing treatment of white lupine seeds with complex compositions based on Tebu 60 fungicide

The results of laboratory and field tests of effectiveness of the Tebu 60 protectant, ME (microemulsion) in its pure form and in tank mixtures with Humat+7 and Fitaktiv Vita against anthracnose seed infection and other diseases of lupine are presented. The research was conducted in 2020–2022 in the Bryansk region. The object of the study were the seeds, seedlings and crops of the Michurinsky white lupine variety. In laboratory conditions, biological evaluation of the effectiveness of the Tebu 60 protectant in pure form (rate of consumption – 0.5 l/t) and tank mixtures Tebu 60 + Humate+7 (0.5 + 0.5 l/t) and Tebu 60 + Fitaktiv Vita (0.5 + 0.05 l/t) against anthracnose seed infection was carried out by the number of affected seedlings grown in paper-polyethylene rolls in comparison with the control (without treatment). The tank mixture Tebu 60 + Fitaktiv Vita (100%) showed high biological efficacy against anthracnose. Moreover, this mixture significantly (LSD05 = 0.66) had the least effect on reducing the hypocotyl length of the seedlings (8.8%). The field experiment was laid down in a fourfold repetition. The plot area was 34 m2. The seeding rate was 1.0 million germinated seeds/ha. The soil of the site was gray forest soil, humus content – 2.6%. The forecrop was spring cereal crops. Seed dressing with Tebu 60 (0.5 l/t) and tank mixtures Tebu 60 + Humate+7 (0.5 + 0.5 l/t) and Tebu 60 + Fitaktiv Vita (0.5 + 0.05 l/t) were conducted one month before the sowing. The effectiveness of the treatment was evaluated in comparison with the control. On average, the highest biological efficacy against anthracnose seed infection was shown by the tank mixture Tebu 60 + Fitaktiv Vita (98.1%). By the shiny pod phase, the number of affected beans was 20.5% compared to 78.4% in the control. The biological efficacy against fusarium disease and rhizoctonia was 65.9 and 74.4%, respectively. The prevalence of gray rot and white rot on beans was reduced 8 and 11 times, respectively. Seed germination increased significantly (LSD05 = 0.74) by 16.2%, safety of productive plants to harvesting – by 56.5%. Reliable increase in the seed yield was 2.52 t/ha, cost recovery – 64.88 rubles.

Classification and Severity Level Assessment of Fusarium Wilt Disease in Chickpeas using Convolutional Neural Network

Background: The fusarium wilt disease of chickpea leaves is a common illness that leads to economic problems for farmers due decreased crop yield. Early disease detection and the implementation of suitable precautions can help to increase the yield of chickpeas. This study offers an improved method for Fusarium wilt disease prediction based on severity level using a convolutional neural learning algorithm. Methods: The Convolutional Neural Network (CNN) model is utilized in this work to identify leaf disease due to wilting. The dataset contains 4,339 images of chickpea leaves that were obtained from Kaggle. After preprocessing, the data is sent into the network model for training. The model shows acceptable classification and accuracy metrics. Result: Deep learning methods are very useful tools for tracking leaf diseases at their early stages and can help farmers with the use of controlling methods. The proposed work looks for changes in the shape and color of chickpea leaves in order to predict severe fusarium disease. Training and validation accuracies show a balanced trade-off by giving satisfactory outcomes. The model shows an overall accuracy of 74.79%. The confusion matrix and classification parameters increase the model’s performance.

A potent endophytic fungus Purpureocillium lilacinum YZ1 protects against Fusarium infection in field-grown wheat.

Fusarium diseases pose a severe global threat to major cereal crops, particularly wheat. Existing biocontrol strains against Fusarium diseases are believed to primarily rely on antagonistic mechanisms, but not widely used under field conditions. Here, we report an endophytic fungus, Purpureocillium lilacinum YZ1, that shows promise in combating wheat Fusarium diseases. Under glasshouse conditions, YZ1 inoculation increased the survival rate of Fusarium graminearum (Fg)-infected wheat seedlings from 0% to > 60% at the seedling stage, and reduced spikelet infections by 70.8% during anthesis. In field trials, the application of YZ1 resulted in an impressive 89.0% reduction in Fg-susceptible spikelets. While a slight antagonistic effect of YZ1 against Fg was observed on plates, the induction of wheat systemic resistance by YZ1, which is distantly effective, non-specific, and long-lasting, appeared to be a key contributor to YZ1's biocontrol capabilities. Utilizing three imaging methods, we confirmed YZ1 as a potent endophyte capable of rapid colonization of wheat roots, and systematically spreading to the stem and leaves. Integrating dual RNA-Seq, photosynthesis measurements and cell wall visualization supported the link between YZ1's growth-promoting abilities and the activation of wheat systemic resistance. In conclusion, endophytes such as YZ1, which exhibits non-antagonistic mechanisms, hold significant potential for industrial-scale biocontrol applications.

In vitro antifungal activities of dose-dependent bioactive compound from potato peels to combat powdery mildew disease in cucumber

The search for biopolymer-based materials to replace dangerous chemicals in agriculture has become increasingly important in recent years for sustainable crop development. In our study enzymatic synthesis of isomaltose was done using potato peels. Isomaltose solvent extracts were tested for their ability to inhibit fungal pathogens like Erysiphe cichoracearum and Fusarium oxysporum. Among those tested, a dosedependent isomaltose extract exhibited significant inhibition on E. cichoracearum and F. oxysporum pathogen conidial sporulation, number of conidia and their motility. Under the in vitro studies, with isomaltose extract at (2.5 mg mL ) recorded a maximum conidial inhibition of 86.2, ?1 E. cichoracearum average number of conidia 4-27 and 5 per cent motility. While, sterile distilled water and DMSO showed maximum growth of the both the pathogens and also their motility was 92-97 per cent, respectively. Similarly, F. oxysporum maximum conidial inhibition of 88.3 and average conidia of 9-30 with motility of 7 per cent was observed after treating with 2.5 mg mL isomaltose. Moreover, isomaltose a biopolymer ?1 derived from potato peel was used in different concentrations as priming agent to cucumber seeds and were evaluated for its effectiveness to enhance plant growth parameters. Among the treatments, seeds-primed with 2.5 mg mL of isomaltose extracts in water exhibited early seedling germination of 96.8 per cent and ?1 vigor of 2210.3 and also cucumber seeds treated with 2.5 mg mL of isomaltose extracts in DMSO showed ?1 significant germination rate of 95.5 per cent and vigor index of 1987.7. Overall, these results determine that the antifungal property of water dissolved isomaltose is highly significant in exhibiting remarkable inhibitory action against Fusarium and powdery mildew phytopathogens, further the dose-dependent concentration of water dissolved isomaltose showed fair to good enhancement of seed germination which will lead to establishing a novel bio-waste agricultural formulation for the management of plant pathogens and in particular Fusarium and powdery mildew disease of cucumber and other cucurbitaceous crops.. KEYWORDS :Bio-polymer, optimize, pathogen inhibition, plant growth, seed treatment

Recent Advances in Understanding and Controlling Fusarium Diseases of Alliums

Allium species are known for their culinary, medicinal, and ornamental purposes. Fusarium basal rot is one of the most damaging soilborne fungal diseases of Allium species and poses a significant threat to yield, quality, and storage life worldwide. Various species of Fusarium have been identified as causal agents for Fusarium basal rot, depending on the Allium species involved. Diverse disease management practices have been implemented to mitigate the impact of Fusarium basal rot. This review article provides a comprehensive overview of the recent progress in detecting different species of Fusarium involved in Fusarium basal rot and strategies to control them in affected Allium species involving chemical, biological, and cultural methods. It covers the latest advancements in host plant resistance research from traditional breeding to modern molecular techniques and studying secondary metabolites involved in defense mechanisms against Fusarium basal rot.

FvMbp1-Swi6 complex regulates vegetative growth, stress tolerance, and virulence in Fusarium verticillioides

The mycotoxigenic fungus Fusarium verticillioides is a common pathogen of grain and medicine that contaminates the host with fumonisin B1 (FB1) mycotoxin, poses serious threats to human and animal health. Therefore, it is crucial to unravel the regulatory mechanisms of growth, and pathogenicity of F. verticillioides. Mbp1 is a component of the MluI cell cycle box binding factor complex and acts as an APSES-type transcription factor that regulates cell cycle progression. However, no information is available regarding its role in F. verticillioides. In this study, we demonstrate that FvMbp1 interacts with FvSwi6 that acts as the cell cycle transcription factor, to form the heteromeric transcription factor complexes in F. verticillioides. Our results show that ΔFvMbp1 and ΔFvSwi6 both cause a severe reduction of vegetative growth, conidiation, and increase tolerance to diverse environmental stresses. Moreover, ΔFvMbp1 and ΔFvSwi6 dramatically decrease the virulence of the pathogen on the stalk and ear of maize. Transcriptome profiling show that FvMbp1-Swi6 complex co-regulates the expression of genes associated with multiple stress responses. These results indicate the functional importance of the FvMbp1-Swi6 complex in the filamentous fungi F. verticillioides and reveal a potential target for the effective prevention and control of Fusarium diseases.

Utilization of a Novel Soil-Isolated Strain Devosia insulae FS10-7 for Deoxynivalenol Degradation and Biocontrol of Fusarium Crown Rot in Wheat.

Deoxynivalenol (DON) is the most widespread mycotoxin contaminant hazardous to human and animal health globally. It acts as a crucial virulence factor to stimulate the spread of pathogenic Fusarium within wheat plants. Control of DON and Fusarium disease contributes enormously to food safety, which relies on chemical fungicides. Here, we report the biodegradation of DON using a novel soil bacterium, Devosia insulae FS10-7, and its biocontrol effect against Fusarium crown rot. We demonstrated that strain FS10-7 degraded DON to 3-epi-DON by forming a 3-keto-DON intermediate. Such degradation activity can be maintained at a wide range of pH (4 to 10) and temperature (16 to 42°C) values under aerobic conditions. Notably, strain FS10-7 exhibited practical inhibitory effects on Fusarium crown rot disease caused by F. graminearum and F. pseudograminearum in the in vitro Petri dish test under laboratory conditions and the pot experiment under greenhouse conditions. The mechanisms underlying the biocontrol ability of strain FS10-7 were preliminarily investigated to be associated with its high DON-degrading activity rather than direct antagonism. These results establish the foundation to develop further bioagents capable of biodegrading mycotoxins in cereals and derived products and, accordingly, biocontrol plant diseases caused by DON-producing pathogens.

Effect of Preprocessing and Augmentation Process in Development of a Deep Learning Model for Fusarium Detection in Shallots

As the demand for shallot increases, wide-scale cultivation area must be managed efficiently. However, shallot productivity decreases every year because of plant diseases. Fusarium disease has an intensity up to 60% and can affect yield losses up to 50%. This study was conducted to develop the fusarium disease detection system for shallot using deep learning model and analyze the effect of preprocessing and augmentation adjustment. This study used YOLOv5 deep learning algorithm consisting of the following stages: (1) dataset acquisition, (2) dataset annotation, (3) dataset preprocessing and augmentation, (4) dataset training and validation, and (5) model testing and evaluation. A total 9,664 annotated dataset was trained to YOLOv5m pre-trained weights. Based on testing and evaluation results, precision, recall, and mean average precision (mAP) metrics of the model without preprocessing and augmentation were 55.5%; 54%; and 48.3% respectively. Metric values of the model were increased to 57.6%; 58.4%; and 54.1% respectively with adjustment of preprocessing and augmentation combination process. Percentage increase in metrics when compared to the control model for each value of precision, recall, and mAP were 2.1%; 4.4%; and 5.8%. This shows a significant impact on the addition of preprocessing and augmentation processes that match the characteristics of the dataset to increase the value of model performance. Keywords: Augmentation, Deep learning, Fusarium, Shallot.

The Interaction between Hypovirulence-Associated Chrysoviruses and Their Host Fusarium Species.

Chrysoviruses are isometric virus particles (35-50 nm in diameter) with a genome composed of double-stranded RNAs (dsRNA). These viruses belonged to the Chrysoviridae family, named after the first member isolated from Penicillium chrysogenum. Phylogenetic classification has divided the chrysoviruses into Alphachrysovirus and Betachrysovirus genera. Currently, these chrysoviruses have been found to infect many fungi, including Fusarium species, and cause changes in the phenotype and decline in the pathogenicity of the host. Thus, it is a microbial resource with great biocontrol potential against Fusarium species, causing destructive plant diseases and substantial economic losses. This review provides a comprehensive overview of three chrysovirus isolates (Fusarium graminearum virus 2 (FgV2), Fusarium graminearum virus-ch9 (FgV-ch9), and Fusarium oxysporum f. sp. dianthi mycovirus 1 (FodV1)) reported to decline the pathogenicity of Fusarium hosts. It also summarizes the recent studies on host response regulation, host RNA interference, and chrysovirus transmission. The information provided in the review will be a reference for analyzing the interaction of Fusarium species with chrysovirus and proposing opportunities for research on the biocontrol of Fusarium diseases. Finally, we present reasons for conducting further studies on exploring the interaction between chrysoviruses and Fusarium and improving the accumulation and transmission efficiency of these chrysoviruses.

Athogenic properties of pathogens and the damage of straw- berry varieties by mycoses of the root system

Fungal isolates isolated from garden strawberry plants Fusarium semitectum Berk. Et Ravenel and Verticillium albo-atrum Reinke et Berth proved to be pathogenic. Under field conditions, artificial inoculation with these isolates increased the development of verticillium disease to 24.0-57.0 % and the development of fusarium disease to 26.0-31.0 %, which significantly exceeded the growth of root rot in control at the level of 2.0-2.2 %. Inhibition of the above-ground system length in infected plants reached 6.5-30.5 % relative to the control. In a laboratory experiment, the presence of pathogenic properties in an isolate of the fungus Fusarium solani (Mart.) Sacc., also isolated from strawberry plants, was proved. Artificial inoculation caused a decrease in the length of the above-ground system by 33.2 %, the number of new leaves by 53.8%, a reduction in the size of the roots by 43.9 %, and increased rhizome necrosis by 3.2 times relative to the control. The general condition of strawberry plants in the control was satisfactory by 3.2 points. The negative effect of the phytopathogen in variants with inoculation was manifested in a decrease in the state of plants to a level of 1.3-1.4 points. According to the results of a 3-year observation, the varieties Alexandrina, Festivalnaya Romashka, and Solnechnaya Polyanka showed relative resistance to fusarium disease of strawberries (the development of the disease on average over three years ranged from 2.8 to 5.7 %, with 11.3 % in the standard variety Yunia Smaids). Varieties Feya, hybrid Lutova, Aleksandrina, Festivalnaya Romashka, Solnechnaya Polyanka, Festivalnaya, and Anastasia had relative resistance to verticillium disease (the development of the disease on average over three years ranged from 0 to 1.8 %, with 8.9 % for the standard).

Special Issue on Breeding and breeding research for resistance to Fusarium diseases in cereals

Special Issue on Breeding and breeding research for resistance to Fusarium diseases in cereals

Inhibitory activity to Fusarium spp. and control potential for wheat Fusarium crown rot of a novel succinate dehydrogenase inhibitor cyclobutrifluram.

Wheat Fusarium crown rot (FCR) is a serious problem primarily caused by Fusarium pseudograminearum, a pathogenic agent known to produce mycotoxins, including deoxynivalenol (DON). Cyclobutrifluram, a novel succinate dehydrogenase inhibitor devised by Syngenta, has immense potential to control both nematodes and Fusarium diseases. However, its efficacy in combating Fusarium species, its ability to prevent and reverse the detrimental effects of FCR, and its impact on the production of DON by F. pseudograminearum are yet to be fully ascertained. Cyclobutrifluram exhibited substantial inhibitory activity against Fusarium species, with half-maximal effective concentration values ranging from 0.0021-0.0647 μg mL-1 . It demonstrated significant inhibitory activity toward three developmental stages of F. pseudograminearum, F. graminearum and F. asiaticum. Furthermore, cyclobutrifluram showed both protective and curative activities against FCR and was rapidly absorbed by roots and transported to wheat stems and leaves. Cyclobutrifluram could also decrease DON production by F. pseudograminearum. This investigation has revealed the potential of cyclobutrifluram as a formidable candidate fungicide, particularly in its ability to effectively combat FCR and other Fusarium-related ailments. This novel compound has exceptional pathogen-fighting capabilities, coupled with remarkable systemic translocation properties and a notable ability to reduce the production of DON. © 2023 Society of Chemical Industry.

Microbial diversity in soils suppressive to Fusarium diseases.

Fusarium species are cosmopolitan soil phytopathogens from the division Ascomycota, which produce mycotoxins and cause significant economic losses of crop plants. However, soils suppressive to Fusarium diseases are known to occur, and recent knowledge on microbial diversity in these soils has shed new lights on phytoprotection effects. In this review, we synthesize current knowledge on soils suppressive to Fusarium diseases and the role of their rhizosphere microbiota in phytoprotection. This is an important issue, as disease does not develop significantly in suppressive soils even though pathogenic Fusarium and susceptible host plant are present, and weather conditions are suitable for disease. Soils suppressive to Fusarium diseases are documented in different regions of the world. They contain biocontrol microorganisms, which act by inducing plants' resistance to the pathogen, competing with or inhibiting the pathogen, or parasitizing the pathogen. In particular, some of the Bacillus, Pseudomonas, Paenibacillus and Streptomyces species are involved in plant protection from Fusarium diseases. Besides specific bacterial populations involved in disease suppression, next-generation sequencing and ecological networks have largely contributed to the understanding of microbial communities in soils suppressive or not to Fusarium diseases, revealing different microbial community patterns and differences for a notable number of taxa, according to the Fusarium pathosystem, the host plant and the origin of the soil. Agricultural practices can significantly influence soil suppressiveness to Fusarium diseases by influencing soil microbiota ecology. Research on microbial modes of action and diversity in suppressive soils should help guide the development of effective farming practices for Fusarium disease management in sustainable agriculture.

Phytosynthesis of silver nanoparticles by Paulownia fortunei fruit exudates and its application against Fusarium sp. causing dry rot postharvest diseases of banana

Over the recent years, banana cultivation has increased in the South-Eastern provinces of Iran. Management of Fusarium diseases in banana poses considerable yield loss as the application of chemical and biological treatments remains challenging. At the present research, about 10 Fusarium isolates causing dry rot on banana fruits were isolated and based on the pathogenicity test, isolate MSN-7 was considered as the most pathogenic isolate. Isolate MSN-7 was identified by using the ITS (ITS1 and ITS4) genomic region. BLAST results showed that it had the highest similarity (99%) with Fusarium chalmydosporum (MN533778.1). The objective of our research was to examine different concentrations of Silver nanoparticles (SNPs) biosynthesized by Paulownia fortunei L.) Fruit Exudates as a novel antifungal agent for reduce of pathogencity of Fusarium dry rot of banana. The synthesis of SNPs was conducted utilizing Paulownia fruit extracts as an environmentally friendly biosource. The exudates derived from Paulownia fruit demonstrated the capability to generate nanoparticles with an average size of 46 nm. Analysis conducted using a transmission electron microscope (TEM) revealed that these nanoparticles possess a polyhedral structure. Furthermore, when applied at concentrations of 10, 20, 40, and 80 μg/ml, these biosynthetic nanoparticles exhibited the ability to inhibit the growth of Fusarium fungus mycelia, as well as induced the destruction of the fungal hyphae.

First Report and Draft Genome Resource of a Unique Opportunistic Fusarium solani Pathogen Associated with Unknown Dark Galls in Sugarbeet

Fusarium disease of sugarbeet is a threat to sugarbeet production and can cause significant losses. During routine evaluation, a unique Fusarium species was solely isolated from unknown dark galls in sugarbeets from Wilkin County, MN, U.S.A. Analysis of the RPB2 gene placed this isolate in the Fusarium solani FSSC 5 clade (100.0% similarity) but uniquely separated from the potato F. solani. Sequencing of this Fusarium species by Minion Nanopore Systems resulted in a draft genome assembly of 19 contigs with a genome size of 59.3 Mb and GC content of 50.66%. A total of 18,900 protein-coding genes, 37 secondary metabolite clusters, 1,559 secreted proteins, 668 carbohydrate-active enzymes, and 404 effectors were predicted in the genome. Pathogenicity tests showed mild vascular discoloration in inoculated sugarbeets unaccompanied by external symptoms, suggesting weak pathogenic ability of this species, indicative of an opportunistic pathogen. These results extend our understanding of this unique opportunistic Fusarium solani isolated from sugarbeet. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Protective Role of Mycosynthesized Bimetallic ZnO-CuO Nanoparticles as Therapeutic Nutrients to Enhance the Resistance of Vicia faba against Fusarium Wilt Disease

The exacerbation of climatic changes helped to increase the risk of plant diseases in the world. The novelty of this study lies in the manufacture of therapeutic nutrients using nanotechnology with strong effectiveness against plant disease. Based on this concept, we mycosynthesized bimetallic ZnO-CuO nanoparticles (NPs), alternatives to reduce the spread of Vicia faba Fusarium wilt disease, which is one of the world’s most imperative cultivated crops. The article’s uniqueness comes in the utilization of ZnO-CuO nanoparticles to carry out two crucial tasks: therapeutic nutrients and managing Fusarium disease. To evaluate the resistance of infected plants, disease index (DI), photosynthetic pigments, osmolytes, oxidative stress and yield parameters were assessed. NPs of ZnO, CuO, and ZnO-CuO were mycosynthesized using a biomass filtrate of Aspergillus fumigatus OQ519856. DI reached 87.5%, due to Fusarium infection, and, as a result, a severe decrease in growth characters, photosynthetic pigments, total soluble carbohydrates, and proteins as well as yield parameters was observed. Infected plants produced more of the studied metabolites and antioxidants. On the other hand, the treatment with CuO-ZnO NPs led to a great decline in the DI by 22.5% and increased the protection by 74.28%. A clear improvement in growth characters, photosynthetic pigments and a high content of carbohydrates and proteins was also observed in both healthy and infected plants as a result of CuO-ZnO NPs treatment. Remarkably, CuO-ZnO NPs significantly increased the yield parameters, i.e., pods/plant and pod weight, by 146.1% and 228.8%, respectively. It could be suggested that foliar application of NPs of ZnO, CuO, and ZnO-CuO could be commercially used as antifusarial agents and strong elicitors of induced systemic resistance.

Ability of arbuscular mycorrhizal to protect tomato (Solanum lycopersicum) seedlings from Fusarium oxysporum

Present experiments were conducted in the microbiology laboratory, Department of Environmental and Agronomic Sciences and in a private nursery in the Tassoust region of Jijel during 2021 and 2022. This study was carried out on the use of strains of arbuscular mycorhizal fungi (Acaulospora sp. and Glomus sp.) as a means to control F. oxysporum effect. After 15 days of inoculation, several measurements of the lengths roots, stem and leaves were noted and symptoms of Fusarium disease were also recorded. The results revealed that F. oxysporum is a pathogen for tomato (Solanum lycopersicum L.) plants (super strain) variety, causing Fusarium disease of this host, which manifests by yellowing, wilting and necrosis of aerial parts, and reduced growth parameters in plants. Therefore, the disease incidence after 15 days of inoculation is estimated at 75 and 50% for (T1 and T3) respectively. In addition, our results revealed that the percentage of incidence was 100 and 25% respectively for T1 and T3 after 22 days. Whereas, the results showed that mycorhizal fungi are associated with the roots of tomato plants. They reduced the incidence rate of Fusarium disease by 50% (T3) and improved the growth of tomato seedlings (Super strain) which manifests itself by a vigorous root system and a very important development of the aerial parts. These results indicate that arbuscular mycorhizal fungi can effectively contribute to the ecological management of soil-borne fungal disease.