Furcation Perforations Research Articles

How Irrigants Affect Mineral Trioxide Aggregate (MTA) Sealing in Furcal Perforations: An In Vitro Study

How Irrigants Affect Mineral Trioxide Aggregate (MTA) Sealing in Furcal Perforations: An In Vitro Study

Comparative evaluation of blood contamination on the push-out bond strength of mineral trioxide aggregate, Biodentine, and bone cement in furcation perforation repair: An in vitro study

ABSTRACT Aim: This in vitro study was designed to compare the effect of blood contamination on the push-out bond strength of mineral trioxide aggregate (MTA), Biodentine, and bone cement. Materials and Methods: Seventy-eight human mandibular molars with furcation were selected and decoronated at the cementoenamel junction. The samples were mounted 3 mm below the furcation area into the acrylic resin. A perforation in the center of the furcation was made using a round carbide bur size 3 with a diameter of 1.2 mm. Then, the perforation was enlarged up to a standardized diameter of 1.3 mm using a size 5 Gates-Glidden drill. The samples were randomly divided into three groups. Based on blood contamination status, each group (n = 26) was further subdivided into two subgroups (n = 13). Whole fresh human blood was obtained from the principal investigator of the research group. A 27-gauge syringe was used to inject blood into the perforated furcal area. A piece of gelatin was packed under the furcal area using a condenser. MTA, Biodentine, and bone cement were mixed according to the manufacturer’s recommendations and compacted into the perforation. A universal testing machine was used to evaluate the push-out bond strength. Statistical Analysis: The results were analyzed using one-way analysis of variance followed by Tukey’s post hoc test (P < 0.05). Results: In the experimental groups with blood contamination, Group 2A exhibited statistically significant push-out bond strength values compared with Group 1A and Group 3A. In the groups without blood contamination, Group 2B showed statistically significant push-out bond strength values compared with Group 1B and Group 3B. Irrespective of the blood contamination status, Group 3 exhibited higher push-out bond strength values than Group 1, with no statistically significant difference between the groups. No significant difference was observed within the groups. Conclusion: Biodentine showed promising results as a better perforation repair material irrespective of the status of blood contamination. Bone cement can be a viable alternative material for furcation perforation repair compared with MTA.

Bond Strength of Composite Resin to Bioceramic Cements: An In Vitro Study

Bioceramic endodontic cements, known for their antibacterial properties, calcium ion release, and alkaline pH, may come into contact with various irrigants after furcal perforation repair. This study aimed to evaluate the effect of different irrigating solutions and setting times on the shear bond strength (SBS) of Biodentine® (Septodont, Saint-Maur-des-Fosses Cedex, France) to a self-adhering flowable composite. Sixty Biodentine® (Septodont, Saint-Maur-des-Fosses Cedex, France) blocks were prepared and divided into two groups based on the setting time: 72 h and 7 days. These were further subdivided into five subgroups based on the irrigation solution applied: distilled water, sodium hypochlorite, ethylenediaminetetraacetic acid, chlorhexidine, and phosphoric acid. They were then restored with Dyad FlowTM (KerrTM, Orange, CA, USA). SBS and failure modes were assessed at 24 h and 6 months. A two-way analysis of variance (ANOVA) test was performed to analyze the effect of the different irrigating solutions and setting times on the SBS of Biodentine® (Septodont, Saint-Maur-des-Fosses Cedex, France) and Dyad FlowTM (KerrTM, Orange, CA, USA). The level of significance was set at a ≤0.05. At 24 h, SBS was significantly influenced by both the irrigant solution (p = 0.029) and setting time (p = 0.018); at 6 months, SBS was influenced only by the irrigating solutions (p < 0.001). The predominant mode of bond failure was adhesive across all groups. In conclusion, while the setting time did not affect the bond strength, certain irrigating solutions reduced it. Thus, careful consideration of surface treatments applied to Biodentine® is crucial for successful endodontic and restorative outcomes.

Assessment of Sealing Ability of Different Bioceramics in Repair of Furcation Perforation: An In Vitro Study

Assessment of Sealing Ability of Different Bioceramics in Repair of Furcation Perforation: An In Vitro Study

Cone Beam Computed Tomography Analysis of Post Space in Bifurcated Premolars Using ParaPost and Peeso Reamer Drills

Introduction Core buildups are usually maintained by the usage of posts. Even so, in curved and narrow canals, such as bifurcated premolars, excessive dentin removal during post-space preparation can result in a vertical root fracture or perforation. In order to preserve root canal dentin during post-space preparation of bifurcated premolars, this study examined and analyzed the effects of two widely used drills: Peeso Reamer (Dentsply Maillefer, Ballaigues, Switzerland) and ParaPost (Coltene/Whaledent, Inc., Altstätten, Switzerland). We also examined the risks associated with using Cone-beam Computed Tomography (CBCT). Methods Three operators with varying levels of experience treated a total of 72 removed bifurcated premolars, dividing them into equal groups. The rotary ProFile system (Dentsply Sirona, Charlotte, NC) was utilized for root canal treatments, and gutta-percha size #30/0.4 was used for obturation. Peeso Reamer #2 and ParaPost #1 were then used to prepare the palatal canals. CBCT was used to assess the total mean intracanal spaces of 3 mm, 5 mm, and 7 mm that were measured both preoperatively and postoperatively. To compare the mean dentin thickness within the canal area across all groups, we used analysis of variance testing. We detected complications, like deviations or perforations, using mesiodistal periapical radiographs. Results When utilizing the Peeso Reamer, the mean dentin thickness (0.749 mm) was slightly more than when using the ParaPost (0.736 mm) with p=0.16. There was no significant difference in the mean dentin thickness of the canal area between the Peeso Reamer and the ParaPost drills. The Pesso Reamer drill had fewer complications since it matched the canal configuration, while the Parapost drill removed somewhat more dentin in the canal area. Only seven teeth with minimum deviation from the center of the canal during preparation were produced by the Peeso Reamer drill (20% of teeth having deviation), whereas twenty-one teeth had deviations (72.2% of teeth) and eight had furcal perforations generated by the ParaPost. Conclusion Within the limitations of our study, the Pesso Reamer drill caused little more dentin removal than the ParaPost drill. However, the Peeso Reamer had fewer risks and was safer to use in bifurcated premolars. The ParaPost drill is not recommended in bifurcated premolars due to the high risks of deviation and perforation because they have narrow canals. The selection of an appropriate drill for post-space use in bifurcated premolars is essential for successful patient outcomes.

Porosity analysis of four bioceramic materials used for the repair of furcation perforations via micro-computed tomography.

Porosity is a crucial parameter that affects the solubility, sealing and mechanical strength of a material. It plays a significant role in determining the success of treatment. The present study aimed to evaluate and compare the porosity of different bioceramic-based materials, using micro-computed tomography (micro-CT). In the study, 76 permanent lower first or second molars that had been extracted for periodontal reasons and were free of calcification, resorption, root cavities, fractures, or cracks, with discrete roots and complete root apex development were selected. In each of the 4 experimental groups, perforations were made in the furcation areas of 19 molars. Mineral trioxide aggregate (MTA) Angelus®, EndoSequence® Root Repair Material (ERRM), Biodentine™, and BioAggregate were placed on the perforated areas of the samples and scanned with a micro-CT to evaluate porosity. The pore volume and the pore percentage with regard to the closed, open and total porosity of these repair materials were calculated individually in each sample. While no statistically significant differences were found between group I (MTA), group III (Biodentine) and group IV (BioAggregate) when evaluating the total pore percentage (p > 0.05), the parameter in group II (ERRM) was found to be significantly lower as compared to other groups (p > 0.05). In comparison with the other materials used in this study, the use of ERRM may be more suitable for perforation repair.

Comparative Evaluation of the Push-Out Bond Strength of Glass Ionomer Cement, Mineral Trioxide Aggregate, Biodentine, and Endosequence Root Repair Material in Repair of Furcation Perforations: An In Vitro Study

ABSTRACT Introduction: An in vitro comparative analysis was performed to calculate the push-out bond strength of commercially existing root repairing cements like glass ionomer cement (GIC), biodentine, mineral trioxide aggregate (MTA), and endosequence root repair material (RRM) employed in furcation perforation, with or without blood contamination present. Materials and Methods: Eighty molars were selected and subjected to furcal perforations. They were categorized based on the cement used for repair (GIC, MTA, biodentine, and endosequence RRM); furthermore, they were sub-divided into two sub-groups, that is, blood contaminated and non-contaminated. For 24 hours, all the samples were kept in an incubator till the materials were fully set. Then these samples were examined for push-out bond strength measurement. Results: The 24-hour push-out bond strength of was the highest in biodentine and the lowest in glass ionomer cement. The push-out bond strength of endosequence RRM, MTA, and GIC was influenced by blood contamination. Conclusion: The push-out bond strength of biodentine was the highest as compared to endosequence RRM, MTA angelus, and GIC. The push-out bond strength of endosequence RRM and MTA angelus after 24 hours with or without blood contamination showed insignificant differences. Group 1A (GIC contaminated with blood) displayed the least push-out bond strength among other groups.

Efficacy of Mineral Trioxide Aggregate (MTA) as a Reparative Material in Iatrogenic Furcal Perforations in Mandibular Molars.

Mineral trioxide aggregate (MTA) is widely recognized as one of the most biocompatible materials for perforation repairs during root canal treatment (RCT). Experimental evidence has consistently demonstrated MTA's superior sealing ability and biocompatibility compared to various dental materials, including amalgam, intermediate restorative material, zinc oxide eugenol cement, and resin-modified glass ionomer cement. This study aimed to assess the efficacy of MTA as a reparative material in iatrogenic furcal perforations during RCT. A descriptive cross-sectional study was conducted from May 18, 2021, to November 17, 2021, at the Department of Operative Dentistry, Nishtar Institute of Dentistry, Multan, Pakistan. Seventy-six patients aged 18-60 years, of both genders, who developed iatrogenic furcal perforations during procedures were included. Patients with fractures or endo-perio lesions identified during clinical and radiographic examinations were excluded. Isolation was achieved using a rubber dam. The perforation site was cleaned and irrigated with 1% sodium hypochlorite to control hemorrhage and enhance visualization. Following the manufacturer's recommendations, the perforation site was sealed with MTA mixed with sterile saline. The age range in this study was 18 to 60 years, with a mean age of 42.09 ± 9.69 years. Most patients (56.78%) were between 41-60 years old. Out of the 76 patients, 46 (60.53%) were male, and 30 (39.47%) were female, resulting in a male-to-female ratio of 1.5:1. The study found that MTA's efficacy as a reparative material in iatrogenic furcal perforations was observed in 61 (80.26%) patients. A 6-month follow-up revealed no periodontal ligament breakdown, demonstrating the efficacy of MTA as a reparative material in iatrogenic furcal perforations. This study concludes that the efficacy of MTA as a reparative material in iatrogenic furcal perforations is remarkable and significant.

The Use of Mineral Trioxide Aggregate and Biodentine in Furcal Perforations Sealing – A Review Article

Abstract Pulp cavity perforation is defined as pathological connection between root canal system and external surface of the tooth. This iatrogenic pulp chamber injury may have serious implications on the success of root canal treatment. The sealing of perforation is crucial for good prognosis of the treated tooth. The ideal material for sealing perforations in the pulp cavity should be characterized by a good adhesion, lack of sensitivity to tissue fluids, volume stability, radiopacity on radiographs and excellent bioactivity and biocompatibility. To date the most well -known bioceramic material to is mineral trioxide aggregate. Due to over thirty years of its application in endodontics, it is called the „gold standard” for perforation repair. Recently, many new bioceramic materials have been developed and introduced to dentistry and endodontics, showing promising clinical results. One of the materials successfully used for pulp chamber floor perforation repair is Biodentine. According to the manufacturers, Biodentine has a much shorter setting time compared to other bioceramic cements, and also has better mechanical properties and is easier to use. The aim of the article is to analyse the available research and compare the properties of those bioceramic materials in a perforation sealing procedure. Based on the analysis of the current literature, it can be concluded that mineral trioxide aggregate is still the most proven and tested material among bioceramic materials, but Biodentine is a good alternative as it is relatively easy to manipulate but also has predictable clinical results.

Sealing ability of Biodentine as a root Perforation Treatment Material (An in Vitro Study)

Perforation of the root canal system is the second large cause of root canal failure. Fa ilure to seal the perforation defect permits rapid break-down of the periodontium and tooth loss. Hence sealing the defect is paramount. Aims: This study was conducted to evaluate the sealing ability of a new material, self-setting dentine substitute (Biodentine) in furcation perforations and compare it with Mineral trioxide aggregate and glass ionomer cement. Materials and Methods: Fifty five (55) recently extracted permanent lower molars with non-fused well developed roots were collected and intentional perforations were made in the furcation area with a bur. The perforations were repaired with Biodentine for group( A) ,mineral trioxide aggregate (MTA) for group (B) and glass ionomer cement(GIC) for group (C). The specimens were then immersed in 2% methylene blue dye for 48 hour. After their removal, they were sectioned and examined under stereo microscope to evaluate dye penetration. Results: demonstrated that furcat ion perforation repaired with Biodentine showed least microleakag e. Conclusion Biodentine demonstrated better results than MTA and GIC.

Effect of Incorporating Titanium Dioxide Nanoparticles into White Portland Cement, White Mineral Trioxide Aggregate, and Calcium Enriched Mixture Cement on the Push-out Bond Strength to Furcal Area Dentin.

Bond strength of furcation repair materials is an essential factor in clinical success. Studies on the effect of adding titanium dioxide (TiO2) nanoparticles on the push-out bond strength of commonly used endodontic cements for furcation perforation repair is limited. This study aimed to evaluate the effect of adding TiO2 nanoparticles to white Portland cement (PC), white mineral trioxide aggregate (MTA), and calcium enriched mixture cement (CEM) on their push-out bond strengths. In this in vitro study, 120 endodontically treated molars were assigned to six groups (n=20) based on the material used to repair the perforation. In three groups, the cements (white PC, white MTA, and CEM) were placed in pure form, and in the three remaining groups, 1 weight % of TiO2 was added. The push-out bond strength was measured using a universal testing machine at a strain rate of 0.5 mm/min. Data were analyzed using one-way ANOVA and post hoc Games-Howell test (p< 0.05). One-way ANOVA showed significant differences in the mean bond strength values between the six groups (p= 0.002). The post hoc Games-Howell test showed that the bond strengths in MTA+TiO2 and PC+TiO2 groups were significantly higher than those in MTA and PC groups, respectively. However, there was no significant difference in the bond strength between CEM and CEM+ TiO2 groups. The incorporation of TiO2 into MTA and PC increased their push-out bond strength. However, it did not affect the push-out bond strength of CEM cement.

Radiographic Evaluation of Furcal Perforation Repair Using e-MTA® in Primary Molars-90 Days Follow-up: A Case Report

Radiographic Evaluation of Furcal Perforation Repair Using e-MTA® in Primary Molars-90 Days Follow-up: A Case Report

Radiographic and immuno-histochemical evaluation of root perforation repair using MTA with or without platelet-rich fibrin or concentrated growth factors as an internal matrix in dog's teeth: in vivo animal study.

To comparatively evaluate the in vivo outcome of MTA repair for contaminated and non-contaminated furcation perforations (FP) with or without PRF and CGF as a matrix in dogs' teeth. Ninety dog teeth were divided into five groups based on the iatrogenic FP repair approach after doing root canal treatment: negative control (without FP), positive control (FP without repair), MTA, MTA + PRF and MTA + CGF groups, where FP were repaired promptly in subdivision 1 (n = 10; non-contaminated) and after 4weeks of oral contamination in subdivision 2 (n = 10;contaminated). After 3months, the perforation site was assessed radiographically (vertical bone density), histologically (inflammatory cell count, epithelial proliferation, cementum and bone deposition) and immunohistochemically (OPN and TRAP antibodies localisation). Data collected were statistically analysed using SPSS software at a 0.05 significance level. The MTA + PRF and MTA + CGF groups demonstrated significantly more bone formation, OPN immunolocalisation and fewer inflammatory cell counts than MTA group. MTA, MTA + PRF and MTA + CGF groups showed significantly favourable radiographic, histological and immunohistochemical healing features than the positive control, especially in non-contaminated subdivisions, that significantly showed better features than the contaminated subdivisions (P < 0.001). The use CGF and PRF as a matrix beneath MTA in FP repair in dog's teeth is promising as it could increase hard and soft tissue regeneration in non-contaminated and contaminated perforations. The repair of FP is challenging especially when associated with contaminated inter-radicular bone loss. Radiographic, histological and immunohistochemical comprehensive evaluation of the root and surrounding attachment apparatus response to different perforation repair protocols could give a predictable clinical outcome.

Evaluation of Sealing Ability of MTA Flow, Biodentine and Pro-Root MTA to Seal the Furcal Perforation with and without Internal Matrix- An In vitro Study.

Endodontic mistakes, also known as procedural accidents, are those bad events that occur during treatment, some of which are attributable to a lack of attention to detail and others of which are completely unforeseeable. The second most frequent reason for root canal failure is perforation. The current in vitro study's objective was to assess the furcal perforation's potential to be sealed with and without internal matrix by MTA flow, Biodentine, and pro-root MTA. 60 samples were allocated into six groups at random (10 each). In intact permanent mandibular first and second teeth, furcal perforation sites were made and sealed with various materials. Following perforation sealing, the specimens' capacity for sealing was evaluated using the dye penetration method. The current study's findings indicated that Group 2 has the least amount of microleakage and Group 5 has the most. Biodentine has excellent sealing capabilities and can be utilised to heal furcation perforations with or without internal matrix.

The Effects of Tricalcium-Silicate-Nanoparticle-Containing Cement: In Vitro and In Vivo Studies.

A tricalcium-silicate-nanoparticle-containing cement (Biodentine) was developed to overcome the disadvantages of existing mineral trioxide aggregate (MTA) dental materials. This study aimed at evaluating the effects of Biodentine on the osteogenic differentiation of human periodontal ligament fibroblasts (HPLFs) in vitro and the healing of furcal perforations created experimentally in rat molars in vivo, in comparison to MTA. The in vitro studies performed the following assays: pH measurement using a pH meter, the release of calcium ions using a calcium assay kit, cell attachment and morphology using SEM, cell proliferation using a coulter counter, marker expression using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and cell mineralized deposit formation using Alizarin Red S (ARS) staining. In the in vivo studies, MTA and Biodentine were used to fill the rat molar perforations. Rat molars were processed at 7, 14 and 28 days for analysis of inflammatory processes using hematoxylin and eosin (HE) staining, immunohistochemical staining of Runx2 and tartrate-resistant acid phosphate (TRAP) staining. The results demonstrate that the nanoparticle size distribution of Biodentine is critical for osteogenic potential at an earlier stage compared to MTA. Further studies are required to elucidate the mechanism of action of Biodentine in osteogenic differentiation.

MANAGEMENT OF FURCAL PERFORATION USING BIODENTINE – A CASE REPORT

Management of furcal perforation in a root canal retreatment is often challenging as the outcome of the perforation sealing depends on several parameters. The introduction of several bioactive cements and magnication systems has made the sealing of perforations easier and the outcome more predictable. Current advances in endodontics and biomaterials has made the recovery of tooth structure and function possible even in the most complicated cases. Despite the breakthroughs in techniques and resources, furcal repair remains taxing. This case report shows successful management of multiple furcal perforation with a questionable prognosis in root canal retreatment using biodentine.

Assessment of the diagnostic accuracy of strip and furcal perforations in different sizes by cone beam computed tomography.

Root perforation is an adverse event that may accidentally occur during root canal treatment and can adversely affect the treatment plan and tooth prognosis. This study aimed to assess the accuracy of cone beam computed tomography (CBCT) for detecting the strip and furcal perforations with different sizes. The mesiolingual canals of 155 extracted human mandibular first molars were instrumented and randomly divided into four experimental groups and one control group (n = 31). Furcal (in the pulp chamber floor) and strip perforations with 0.5, 1, 1.5, and 2mm in diameter were manually created. The teeth were randomly mounted in bovine ribs and scanned using CBCT. Two radiologists unaware of the study groups observed the images and reported the greatest perforation diameter. The inter-observer agreements were calculated. The diagnostic accuracy was compared for furcal and strip perforations with different sizes. The inter-observer agreement for detecting furcal and strip perforations with different sizes were good. The diagnostic accuracy was 100% for the absence of perforation in each group. CBCT can detect the absence of perforation with high accuracy. The diagnostic accuracy of CBCT in the presence of strip and furcal perforation with different sizes was not significant.

In Vitro Assessment of Sealing Ability of Various Materials Used for Repair of Furcal Perforation: A SEM Study.

The aim of the current in vitro research was to evaluate the sealing capacity of three different agents employed for the repair of perforations at the furcation area. Recently 60 extracted human mandibular permanent molars having well apart plus fully formed roots, and intact furcation were chosen. The 60 samples were allocated at random to three groups of 20 samples: Group I: Furcation perforation repair by means of mineral trioxide aggregate (MTA)-Angelus, Group II: Furcal perforation repair using Biodentine, Group III: Furcal perforation repair by EndoSequence. The specimens were subjected to sectioning with a hard tissue microtome and the sectioned parts of the samples were then examined. The specimens were subjected to gold sputtering and visualizing beneath scanning electron microscope (SEM) at 2000× magnification for assessing the sealing capacity of the agents. The highest sealing capacity was noted with the use of Biodentine at 0.96 ± 0.10, in pursuit by EndoSequence use at 1.18 ± 0.14 and MTA-Angelus use at 1.74 ± 0.08. The disparity amid the three groups was statistically significant with p < 0.001. In conclusion, it may be inferred that Biodentine exhibited the finest sealing capacity than EndoSequence and MTA- Angelus. It may thus be given consideration as a substance of preference for the repair of furcal perforation. Using biologically compatible substances may be suggested to amend perforations thereby decreasing the occurrence of inflammatory response in the neighboring tissues. The sealing capacity is a significant feature in supporting the result of a root canal treatment of a tooth.

A scanning electron microscopic study evaluating the sealing ability of MTA, BiodentineTM, and new light-cure MTA used for furcal perforation repair.

To evaluate and compare the sealing ability of Mineral trioxide aggregate (MTA), BiodentineTM and light cure MTA used for the repair of furcal perforations using scanning electron microscope (SEM). The study sample comprised 45 extracted mandibular molars. The teeth were embedded in modeling wax. Standard access cavities were prepared in each tooth using a round bur and non-end cutting bur with a high-speed handpiece with water spray. Furcal perforations were made on the centre of the pulpal floor of each tooth using a 0.5mm round bur. The teeth were then randomly divided into 3 experimental groups of 15 specimens each based on the materials used to seal the perforation; Group A: MTA; Group B: BiodentineTM; Group C: Light cure MTA. All the sealed perforations were compacted with a moist cotton pellet, and the samples were stored in a closed container for 24 hours to allow the repair materials to set completely. After 24 hours the samples were sectioned longitudinally and the extent of marginal adaptation was measured using scanning electron microscopy. The sealing ability was evaluated by measuring the gap (in microns) between the pulpal floor and the material used for the furcal repair. The overall results showed that the marginal adaptation of light-cure MTA was better than both MTA as well as Biodentine. The mean space between the pulpal floor and the repair material was least for group C (2.29). Tuckey's post hoc test showed that a significant difference (p<0.05) existed between group C and group A & B. Light-cure MTA exbibits good sealing ability to dentin when compared to conventionally used Biodentine and Mineral Trioxide Aggregate. Key words:Calcium silicates, MTA, Biodentinte, New light-cure MTA, Perforation repair.

Comparative Evaluation of Various Root Canal Irrigants on the Marginal Integrity of Furcal Perforation Repair Material: An In-vitro Study

Introduction: Furcal perforations can occur during access cavity preparation while locating the canal orifices. This must be sealed immediately. After the repair of furcal perforation, endodontic treatment should be performed with various irrigants to clean the root canal system. This procedure causes unavoidable contact of endodontic irrigants with the site of furcal repair. Aim: To evaluate the effect of root canal irrigants on the marginal integrity of furcal perforation repair material using protein leakage assessment. Materials and Methods: This in-vitro study was conducted from June to September of 2021 in Kalinga institute of dental sciences, KIIT University, Bhubaneswar, Odisha, India. A total of 90 extracted mandibular molars with intact furcation were used. Access cavities were prepared. Based on the repair materials, samples were randomly divided into two groups. An artificial perforation of diameter 2 mm was made in the furcation area. The specimens were divided according to the furcation perforation repair materials used: Biodentine, Endosequence (n=45 each). Perforations were filled with Biodentine, Endosequence. They were then subdivided into three subgroups, each containing samples of (n=15) according to the irrigating solutions used. Each group was irrigated with 0.2% Chitosan, Chloroquick, and 5.25% Sodium Hypochlorite (NaOCl) for two minutes, respectively. Protein {Bovine Serum Albumin (BSA)} microleakage was checked by preparing apparatus having upper, lower chamber. Protein leakage through the furcation repair material into the lower chamber was assessed by Ultraviolet (UV) visible spectrophotometry. The microleakage was assessed with a reagent Coomassie Brilliant BlueG-250 daily for 60 days. Intergroup comparisons were made using one way Analysis of Variance (ANOVA) Test. The multi group comparisons were made using Tukey Honestly Significant Difference (HSD) tests. Results: A 0.2% Chitosan showed more protein leakage than Chloroquick over a period of 60 days (p&lt;0.0001), as compared to the baseline, 30 days values with both the furcation repair materials. A 5.25% NaOCl irrigated samples exhibited highest protein leakage among all the irrigants. Conclusion: Biodentine has a better sealing ability than Endosequence BC sealer. Chloroquick proved to be the better irrigant as compared to Chitosan, Hypochlorite in terms of affecting sealing of furcation repair materials.