Distribution Of Fungal Species Research Articles

Culture-based diversity of endophytic fungi of three species of Ferula grown in Iran

A total of 1,348 endophytic fungal strains were isolated from Ferula ovina, F. galbaniflua, and F. persica. They included Eurotiales (16 species), Pleosporales (11 species), Botryosphaeriales (1 species), Cladosporiales (2 species), Helotiales (6 species), Hypocreales (31 species), Sordariales (7 species), Glomerellales (2 species), and Polyporales (1 species). F. ovina had the richest species composition of endophytic fungi, and the endophytic fungi were most abundant in their roots compared to shoots. Chao, Margalef, Shannon, Simpson, Berger–Parker, Menhinick, and Camargo indices showed that F. ovina roots had the most endophytic fungal species. The frequency distribution of fungal species isolated from Ferula spp. fell into the log-series model, and F. ovina roots had the highest Fisher alpha. The dominance indices showed that there are no dominant species in the endophytic fungal community isolated from Ferula spp., indicating community stability. Evenness values were 0.69, 0.90, 0.94, and 0.57 for endophytic fungi isolated from F. ovina roots, F. ovina shoots, F. galbaniflua roots, and F. persica roots, respectively, indicating a species distribution that tends toward evenness. The fungal species community isolated from each of F. ovina roots, F. ovina shoots, F. galbaniflua roots, and F. persica roots was a diverse species group originating from a homogeneous habitat. Their distribution followed a log-normal distribution, suggesting that the interactions of numerous independent environmental factors multiplicatively control species abundances. Principal component analysis showed that the highest species diversity and dominance were observed in the endophytic fungal community isolated from F. ovina and F. persica roots, respectively.

Diversity and Composition of Belowground Fungal Communities Associated with Picea abies L. (H.) Karst. and Larix sp. Mill.: A Comparative Study

The aim of the presented study was to compare the diversity and composition of fungal communities associated with the roots and the rhizosphere soil of P. abies and Larix sp. in mid-age and mature managed forest stands in Lithuania. We also aimed to assess the presence of fungi–host-specific associations, i.e., whether Larix sp. stands could provide habitats for soil fungi currently associated with P. abies. The study sites were 10 Larix sp. and 10 P. abies forest stands in Lithuania. For the study, 100 root samples and 10 organic and 10 mineral soil samples were collected in P. abies stands as well as the same number in Larix sp. stands, and DNA was isolated, amplified using ITS2 rDNA as a marker and subjected to high-throughput sequencing. The results showed that the Shannon diversity index of fungal communities was similar between the two tree species when compared either between root (H = 4.26 P. abies and H = 3.82 Larix sp.), organic soil (H = 5.12 P. abies and H = 5.13 Larix sp.) or mineral soil (H = 4.71 P. abies and H = 4.29 Larix sp.) samples. Multivariate analysis showed that the fungal community composition in the organic and mineral soil samples of both P. abies and Larix sp. were similar, and thus, overlapping. The analysis also showed that the distribution of fungal species was denser in the roots and organic soil but more scattered in mineral soil. However, several fungi in the roots of either P. abies or Larix sp. showed a certain host specificity.

Exploring the Diversity and Systematics of Phaeosphaeriaceae: Taxonomic Novelties from Ecologically Diverse Habitats and Their Phylogenetic Resolution.

The family Phaeosphaeriaceae is a diverse group of ascomycetous fungi that are commonly found in terrestrial habitats worldwide. In recent years, there has been increasing interest in the biodiversity of Phaeosphaeriaceae in China, particularly in Sichuan Province, which has not been fully explored for its high fungal diversity. In our study, we conducted extensive surveys in Sichuan Province to identify and describe new species of Ascomycota with diverse habitats. Here, we present a taxonomic revision of Phaeosphaeriaceae with taxonomic novelties from terrestrial habitats in Sichuan Province based on a multi-gene phylogenetic approach. Our study focuses on the description of four new species of Phaeosphaeriaceae, representing novel additions to the diversity of this fungal family. Using a combination of morphological and molecular data, we provide detailed descriptions of the new taxa and their placement within the family. Additionally, we discuss the phylogenetic relationships of these new taxa with other members of Phaeosphaeriaceae, providing insights into the correct taxonomic classification of the family. Our taxonomic revision contributes to understanding fungal diversity in China and provides a foundation for future studies investigating the taxonomy and ecological roles of Phaeosphaeriaceae fungi. Furthermore, our multi-gene phylogenetic approach provides increased resolution and accuracy in the delimitation of species boundaries within the family. Our study highlights the importance of continued exploration and taxonomic revision in order to fully understand the diversity and distribution of fungal species in China and beyond. New species:Paraloratospora sichuanensis, Phaeosphaeria chengduensis, P. sichuanensis, and Septoriella shoemakeri. New combinations:Paraloratospora breonadiae, P. fructigena, Septoriella ammophilicola, S. asparagicola, S. festucae, S. luzulae, and S. verrucispora. New names:Septoriella paradactylidis, and S. neomuriformis.

Role of Fungal Species Distribution in the Chilli and sesame crop production

Fungi are heterotrophic microorganisms; provide very valuable support for the crop production. Especially, soil fungi play a significant role as one of the key decomposers in the soil environments. During our recent work, soil samples of two vegetable crop grounds viz., Green chilli and Sesame were analyzed for the enumeration and isolation of fungal community based on their accessibility apart from the soil texture and nutrient. Altogether 141 colony forming units (CFUs) of fungi were sequestered from the soil sample of both the cultivated crop fields. In fungal composition, total 16 species belonged to 9 genera were documented from the soils of both crop fields. Soil of green chilli field contributed (77%), the maximum fungal inhabitants followed by sesame field soil (23%). Among the isolated fungi, Aspergillus terreus was established as the dominant fungus in green chilli soil but Aspergillus niger was the dominant fungus in sesame field soil. Result clearly specified that in both the crop fields, Populospora and Trichoderma spp were recorded in negligible, which is recognized as a negative sign for the plant growth and in their production rate due to the affect by fungal diseases. The study was further repeated in keeping the seeds of sesame and chilli in the broth culture of Trichoderma sp. for 72 hours to form a mycelial form over the seed and then the seeds were sown and checked the production rate and growth period. Diversity of soil fungi was found higher in the green chilli field as compared to the sesame field which enhances the production rate up to 1.5 fold.

How Deep Can the Endophytic Mycobiome Go? A Case Study on Six Woody Species from the Brazilian Cerrado

Elucidating the complex relationship between plants and endophytic fungi is very important in order to understand the maintenance of biodiversity, equity, stability, and ecosystem functioning. However, knowledge about the diversity of endophytic fungi from species of the native Brazilian Cerrado biome is poorly documented and remains largely unknown. These gaps led us to characterize the diversity of Cerrado endophytic foliar fungi associated with six woody species (Caryocar brasiliense, Dalbergia miscolobium, Leptolobium dasycarpum, Qualea parviflora, Ouratea hexasperma, and Styrax ferrugineus). Additionally, we investigated the influence of host plant identities on the structure of fungal communities. Culture-dependent methods coupled with DNA metabarcoding were employed. Irrespective of the approach, the phylum Ascomycota and the classes Dothideomycetes and Sordariomycetes were dominant. Using the cultivation-dependent method, 114 isolates were recovered from all the host species and classified into more than 20 genera and 50 species. Over 50 of the isolates belonged to the genus Diaporthe, and were distributed into more than 20 species. Metabarcoding revealed the phyla Chytridiomycota, Glomeromycota, Monoblepharomycota, Mortierellomycota, Olpidiomycota, Rozellomycota, and Zoopagomycota. These groups are reported for the first time as components of the endophytic mycobiome of Cerrado plant species. In total, 400 genera were found in all host species. A unique leaf endophytic mycobiome was identified in each host species, which differed not only by the distribution of fungal species, but also by the abundance of shared species. These findings highlight the importance of the Brazilian Cerrado as a reservoir of microbial species, and emphasize how endophytic fungal communities are diversified and adapted.

Clinical presentation and fungal species distribution in otomycosis in a tertiary care hospital

Objective: Otomycosis is a common fungal ear infection in tropical and subtropical regions worldwide. This study aimed to perform mycological analysis on fungal debris from the external auditory canals of the patients to study the most common clinical presentation and fungal species distribution in otomycosis. Materials and Methods: Patients who met the inclusion criteria for this cross-sectional study were included and evaluated after providing written informed consent. After obtaining a thorough medical history, ear swabs for culture, sensitivity, and potassium hydroxide were provided. Patients with positive culture results were studied between September 2019 and March 2021. Results: Among 103 cases observed for 18 months in the Department of ENT, Rajarajeswari Medical College, and Hospital, Bengaluru, India, we found that males (56.31%) were more affected than females (43.68%). Itching (67.96%) was the most primary and common symptom that was observed, followed by pain (20.38%), and the most common predisposing factor was the usage of earbuds (26.21%) followed by water in the ear (23.3%) and oil in the ear (16.50%). Unilateral infection was most common (96%), and the left ear was most affected (64.07%). Aspergillus niger was the most common fungal isolate (60.19%), and otomycosis was very common in postmonsoon (October–December) (58.25%). Conclusion: The most frequent fungal isolates in otomycosis are from the Aspergillus and Candida species. The left ear was typically affected by otomycosis, which frequently had a unilateral predominance. The most common clinical symptoms were itching and pain.

DIVERSITY OF FUNGI IN THE CARPATHIAN MOUNTAINS: LITERATURE REVIEW AND INVESTIGATION OF CURRENT BIOLOGICAL AND ECOLOGICAL ASPECTS

Biodiversity conservation is one of the fundamental prerequisites for sustainable environmental development. This research aims to study the diversity of fungi in the Carpathian Mountains and their distribution across major biotopes, analyze the results of anthropogenic influence, and develop practical adaptation strategies. The study employed general scientific methods of cognition, including systematic analysis and synthesis, specification, deduction, generalization, abstraction, and formalization. This article critically synthesizes the species composition of fungi in the Ukrainian Carpathians, investigates the distribution of fungal species within their ranges, and assesses the current coverage of this topic in scientific literature. The research aimed to establish a system of regularities and peculiarities of the research object, such as the impact of climate change and anthropogenic pressure on fungal diversity in the Ukrainian Carpathians. The formalization method was employed to formulate priority vectors for stabilizing and optimizing mycocoenoses in the Ukrainian Carpathians and to document the research results for active practical implementation in the region's environmental optimization process. A system of preventive measures is proposed as part of the strategy for adapting the environment to anthropogenic-climatic dynamics. Key measures for optimizing the ecological situation in the field of mycology in the region have been developed. The practical significance of the obtained results lies in their potential use for studying the dynamics of fungal diversity in the Ukrainian Carpathians during periods of global climate change. This research also contributes to developing adaptation strategies at regional and national levels and forming a comprehensive approach to biodiversity preservation in protected areas.

P069 Caspian Sea Mycobiome: The variety and abundance of medically important fungi in beach sand and water

Poster session 1, September 21, 2022, 12:30 PM - 1:30 PM ObjectivesThe Caspian Sea is considered the largest closed water body on the surface of the earth and its isolation has turned it into a very special ecosystem. Keeping the value of these highly appreciated areas for leisure and amusement is of extreme importance for the coastal communities. Regarding the importance of the issue, the present work reports on the distribution and the antifungal susceptibility profile of fungi collected from Caspian Sea beach sand and water.MethodA total of 52 stations distributed within 32 cities along the Caspian Sea coastline in the North of Iran were evaluated. Samples were collected during the peak of summer 2021 (highest concentration of users) from June 1 to September 15 2021 on sunny days between 11 a.m. to 2 p.m. Four samples were collected from each station including dry sand collected from the middle of the dry sand section of the shore; wet sand comprising coastline areas sand and the shallow part (20 cm in depth) of seaboard ∼ 1m toward the seawater; and finally, water sample which was gathered from the shoreline. The grown samples were primarily analyzed according to the macro/microscopic morphologic features of the grown fungi. Also, molecular identification was performed for yeasts and Aspergillus sp. strains using the PCR-RFLP method by digesting beta-tubulin and ITS regions with Alw1 and Msp1 restriction enzymes, respectively. Antifungal Susceptibility tests were performed using CLSI M38, M60, and M59 for probable-isolated Aspergillus and Candida sp., respectively.ResultsFrom the 212 analyzed samples, 125 (58.9%) were positive for the fungal species comprising yeasts and yeast-like fungi (n = 18/14.4%) (C. albicans: n = 7, C. tropicalis: n = 2, other yeast species: n = 7/Trichosporon sp. n = 2), potential pathogenic black fungi (n = 17/13.6%) (Bipolaris sp. n = 2, Cladosporium sp. n = 3, other dematiaceous fungal species n = 12); and potential pathogenic hyaline fungi (n = 92/73.6%) (A. flavus/oryzae n = 34, Aspergillus section nigri n = 14, A. nidulans n = 5, A. fumigatus n = 2, Penicillium sp. n = 11, Fusarium sp. n = 3, Trichoderma sp. n = 18, Mucor sp. n = 3, Geotrichom sp. n = 2). A high variety of fungal species distribution was reported from wet sand samples rather than the other. Amongst, A. flavus/oryzae was the predominant species isolated from different type samples. Regarding M59, a rate of 9.0%, 3.6%, and 1.2% of Aspergillus section nigri, A. flavus/oryzae and A. fumigatus isolates were non-wild types against PSZ, respectively. In the case of ITZ, VRZ, and ISZ, only 1.2%, 1.2%, and 3.6% of total Aspergillus isolates were reported as non-wild type, respectively. All Candida isolates were wild type strains. According to GM values, EFZ and AMB were the most effective agents against yeasts, hyaline, and dematiaceous filamentous fungi.ConclusionIsolation of potentially pathogenic fungi especially those which have developed resistance against antifungals is of great concern for both beachgoer health and the coastal environment sanitation. Therefore, beach sand should be screened regularly for a variety of potentially pathogenic fungi which are considered to be harmful to human health, especially to whom they suffered from any kind of predisposing factor.

Increased Soil Fertility in Tea Gardens Leads to Declines in Fungal Diversity and Complexity in Subsoils

Soil fungi are key drivers regulating processes between ecosystem fertility and plant growth; however, the responses of soil fungi community composition and diversity in deeper soil layers to the plantation and fertilization remain limited. Using soil samples along with vertical soil profile gradients with 0–10 cm, 0–20 cm, 20–40 cm, and 40–60 cm in a tea garden, we used Illumina sequencing to investigate the fungal diversity and assemblage complexity, and correlated to the low, middle, and high-level fertilize levels. The results showed that the fungal community dissimilarities were different between adjacent forests and tea gardens, with predominate groups changed from saprotrophs to symbiotrophs and pathotrophs after the forest converted to the tea garden. Additionally, the symbiotrophs were more sensitive to soil fertility than pathotrophs and saprotrophs. Subsoil fungal communities present lower diversity and fewer network connections under high soil fertility, which contrasted with the trends of topsoil fungi. Soil pH and nutrients were correlated with fungal diversity in the topsoils, while soil K and P concentrations showed significant effects in the subsoil. Overall, the soil fungal communities in tea gardens responded to soil fertility varied with soil vertical spatial locations, which can be explained by the vertical distribution of fungal species. It was revealed that fertility treatment could affect fungal diversity, and alter network structure and potential ecosystem function in tea garden subsoils.

Habitat Distribution of Fungal Species Collected at Lung NGOC Hoangnature Reserve

Through the survey of 4 different habitats in Lung Ngoc Hoang Nature reserve in Vietnam, totally 63 macro fungi have been found with 313 individuals. On forest habitat, 49 species with 116 individuals were obtained. In which, Pycnoporus sanguineus and Chaetocalathus columellifer had the highest number of individuals with 9 individuals, accounting for 7.76% of the total number of individuals collected in the forest. On the riverside habitat, 47 species with 89 individuals were obtained; The roadside habitat found 71 individuals with 42 species, in these two habitats Pycnoporus sanguineus was the most dominant species. On the agricultural habitat, there are 24 species with 37 individuals out of the total number of individuals collected. In this habitat, species Coprinus disseminates, Chaetocalathus columellifer and Lenzites palisoti were the most dominant with 3 individuals found, accounting for 8.11% of the total population here.

Immunosuppressed Patients with Clinically Diagnosed Invasive Fungal Infections: The Fungal Species Distribution, Antifungal Sensitivity and Associated Risk Factors in a Tertiary Hospital of Anhui Province.

ObjectiveSince the nosocomial fungal infections increasingly emerge, we extensively investigated the fungal species stratification and antifungal sensitivity profiles, clinical characteristics and associated risk factors of immunosuppressed patients with clinically diagnosed invasive fungal infections (IFIs) in a tertiary hospital of Anhui province.MethodsIn total, 112 subjects with immunosuppressive state were enrolled from a comprehensive tertiary hospital in Central China between July 2019 and December 2021. Eight-one fungal isolates were clinically recovered by fungus-culturing approaches. The identifications were conducted through a mass spectrometry detecting platform. The susceptibilities to antifungals were tested using the broth micro-dilution method, and the possible antifungal azole-resistance mechanism in specific Candida species was availably explored by sequencing. Patient medical profiles were accessed via the digitized retrieval system of hospital, from which clinical outcomes and multiple risk factors for immunosuppressed patients with clinically diagnosed IFIs were explicitly documented for evaluation.ResultsCandida species predominated in clinically diagnosed IFIs of immunosuppressed patients (accounting for 88.88%), followed by Trichosporon and Aspergillus species (6.17% and 4.94%, respectively). The source types of specimen were primarily comprised of urine (41.98%), respiratory samples (33.33%) and peripheral blood (9.88%). Frequently isolated Candida and Trichosporon species exhibited a high level of in vitro sensitivity for amphotericin B and 5-fluorocytosine, whereas a substantial portion of Candida species including C. glabrata, C. parapsilosis complex and C. tropicalis, and Trichosporon species showed lowered sensitivity patterns toward itraconazole, fluconazole and voriconazole at different levels. Specifically, gene mutations of ERG11 were identified in azole-resistant C. tropicalis. Distinct risk factors were analyzed to be highly associated with the clinically diagnosed IFI incidence, mainly including hospitalization duration, surgical procedures, immunosuppressive treatments, underlying diseases and other conditions.ConclusionCandida, Trichosporon and Aspergillus species were the top three pathogenic fungal agents causing clinically diagnosed IFIs in immunosuppressed patients. The attenuated sensitivity to azoles in Candida and Trichosporon species needs close surveillance, and ERG11 polymorphism might contribute to azole resistance in specific Candida species. Multiple featured risk factors for immunosuppressed patients developing clinically diagnosed IFIs require further consideration during clinical practice.

New Species and Eight Undescribed Species Belonging to the Families Aspergillaceae and Trichocomaceae in Korea

During a survey of fungal diversity associated with insects, mud, soil, and freshwater niches in different areas in Korea, nine interesting fungal strains were isolated. Based on their morphological characteristics and molecular phylogeny analyses, using a combined data set of β-tubulin (BenA), calmodulin (CaM), and second largest subunit of RNA polymerase (RPB2) sequences, the strains CNUFC AM-44, CNUFC JCW3-4, CNUFC S708, CNUFC WT202, CNUFC AS1-29, CNUFC JCW3-5, CNUFC JDP37, and CNUFC JDP62 were identified as Aspergillus alabamensis, A. floridensis, A. subversicolor, Penicillium flavigenum, P. laevigatum, P. lenticrescens, Talaromyces adpressus, and T. beijingensis, respectively. The strain CNUFC JT1301 belongs to series Westlingiorum in section Citrina and is phylogenetically related to P. manginii. However, slow growth when cultivated on CYA, MEA, CREA is observed and the property can be used to easily distinguish the new species from these species. Additionally, P. manginii is known to produce sclerotia, while CNUFC JT1301 strain does not. Herein, the new fungal species is proposed as P. aquadulcis sp. nov. Eight species, A. alabamensis, A. floridensis, A. subversicolor, P. flavigenum, P. laevigatum, P. lenticrescens, T. adpressus, and T. beijingensis, have not been previously reported in Korea. The present study expands the known distribution of fungal species belonging to the families Aspergillaceae and Trichocomaceae in Korea.

DNA barcode analyses improve accuracy in fungal species distribution models

AbstractSpecies distribution models based on environmental predictors are useful to explain a species geographic range. For many groups of organisms, including fungi, the increase in occurrence data sets has generalized their use. However, fungal species are not always easy to distinguish, and taxonomy of many groups is not completely settled. This study explores the effect of taxonomic uncertainty in databases used for modeling fungal distributions. We analyze distribution models for three morphospecies from the corticioid genus Xylodon (Hymenochaetales, Basidiomycota), comparing models based on species names on vouchers specimens with models derived from species identified by DNA barcode. Differences in the contribution of predictors driving the distribution of each modeled taxon and the extent of their ranges were studied. Records under Xylodon paradoxus, X. flaviporus, and X. raduloides were obtained from fungarium collections and GenBank repository. Two grouping criteria were used: (a) specimens were grouped by their collection or sequence voucher names and (b) specimens were grouped following molecular identification using ITS sequences through barcoding gap species recognition (BGSR). Climatic, geographic, and biotic variables were used to predict the potential distribution of each taxon through MaxEnt algorithm. From the three morphospecies selected according to voucher names, up to 19 species candidates were detected using BGSR. Climatic variables were the most important predictors in distribution models made from names on voucher specimens, but their importance decreased when BGSR was applied. In general, the extent of species distributions was more restricted for taxa under BGSR. Our results show that taxonomic uncertainty has a strong effect in Xylodon species distribution models. Misleading results can be obtained when cryptic species or identification errors mask the actual diversity of the presence records. Preserved specimens in natural history collections offer the possibility to assess whether the species name on labels matches the current species recognition criteria.

Biogeographic Patterns of Arbuscular Mycorrhizal Fungal Communities Along a Land-Use Intensification Gradient in the Subtropical Atlantic Forest Biome.

Information concerning arbuscular mycorrhizal (AM) fungal geographical distribution in tropical and subtropical soils from the Atlantic Forest (a global hotspot of biodiversity) are scarce and often restricted to the evaluation of richness and abundance of AM fungal species at specific ecosystems or local landscapes. In this study, we hypothesized that AM fungal diversity and community composition in subtropical soils would display fundamental differences in their geographical patterns, shaped by spatial distance and land-use change, at local and regional scales. AM fungal community composition was examined by spore-based taxonomic analysis, using soil trap cultures. Acaulospora koskei and Glomus were found as generalists, regardless of mesoregions and land uses. Other Acaulospora species were also found generalists within mesoregions. Land-use change and intensification did not influence AM fungal composition, partially rejecting our first hypothesis. We then calculated the distance-decay of similarities among pairs of AM fungal communities and the distance-decay relationship within and over mesoregions. We also performed the Mantel test and redundancy analysis to discriminate the main environmental drivers of AM fungal diversity and composition turnover. Overall, we found significant distance-decays for all land uses. We also observed a distance-decay relationship within the mesoregion scale (< 104 km) and these changes were correlated mainly to soil type (not land use), with the secondary influence of both total organic carbon and clay contents. AM fungal species distribution presented significant distance-decays, regardless of land uses, which was indicative of dispersal limitation, a stochastic neutral process. Although, we found evidence that, coupled with dispersal limitation, niche differentiation also played a role in structuring AM fungal communities, driven by long-term historical contingencies, as represented by soil type, resulting from different soil origin and mineralogy across mesoregions.

Changing Trends in Isolation Frequencies and Species of Clinical Fungal Strains: What Do the 12-years (2008-2019) Mycology Laboratory Data Tell About?

The frequency and variety of infections caused by fungi are increasing. However, changes and intercenter and regional differences are observed in the distribution of fungal species over the years. It is important to update the epidemiological data in order to enable early and appropriate treatment. In this retrospective study, the number of fungi isolated from clinical samples, their distribution at the genus/ species level and the variations over the years in Hacettepe University hospital which is a regional center for patients at risk of fungal infection were investigated. For this purpose, laboratory records from 2008- 2019 were examined and 21813 fungal strains isolated from 19636 clinical samples were detected. When the first (2008-2013) and second (2014-2019) six-year periods were compared, a 2.5 fold increase was observed in the number of specimens yielding fungal growth (first period; n= 5620, second period; n= 14016). Fungi were most frequently isolated from urine (45.0%), lower respiratory tract (30.7%) and blood (6.8%) samples. Mould isolation rate in all samples increased significantly in the second six-year period (from 8.3% to 10.6%, p≤ 0.001). As expected, the most frequent yeast was Candida albicans (57.0%) and mould was Aspergillus fumigatus complex (50.4%). In the second six-year period, isolation of C.albicans (59.3% to 56.0%, p≤ 0.001) among yeasts and A.fumigatus complex (58.1% to 48.0%, p≤ 0.001) among moulds decreased significantly. In urine specimens, most common fungi were C.albicans (49.8%), Candida glabrata complex (15.6%), Candida tropicalis (8.9%) and Candida kefyr (7.5%). In lower respiratory tract specimens, the most common mould was A.fumigatus complex (51.2%), which has decreased from 63.7% in the first six years to 47.1% in the second period (p≤ 0.001). Over the same period, other Aspergillus species (from 25.5% to 34.1%, p= 0.002) and non-Aspergillus moulds (from 36.3% to 52.9%, p≤ 0.001) were increased. In blood samples, C.albicans (44.4%), Candida parapsilosis complex (21.5%) and C.glabrata complex (13.0%) were the most frequent species. In the second six-year period, the frequency of C.albicans decreased from 47.3% to 42.2% (p= 0.059) and the frequency of C.glabrata complex increased from 9.5% to 15.5% (p≤ 0.001) when compared to the first period. For the sterile specimens other than blood, the most common species were C.albicans (37.8%), C.glabrata complex (9.1%) and C.parapsilosis complex (4.7%). However, the number of fungal isolates and the distribution of the species showed great variation over the years. In our center, a substantial increase in the number of fungal strains isolated from the clinical specimens were observed over a 12-years period. In addition and similar to previously published reports, the increase of strains belonging to species with decreased antifungal susceptibility and/or species with unknown susceptibility were detected. The use of local data is required in order to implement early and appropriate antifungal treatment because of inter-center and regional differences observed in epidemiological trends regarding the distributions of fungal genera and species. Surveillance studies to be conducted with the participation of large and sufficient numbers of centers in our country, as we have done for our center, will also contribute to approaches regarding the management of fungal infections by revealing the epidemiological data in a comprehensive manner.

Fungal Community in Antarctic Soil Along the Retreating Collins Glacier (Fildes Peninsula, King George Island).

Glacial retreat is one of the most conspicuous signs of warming in Antarctic regions. Glacier soils harbor an active microbial community of decomposers, and under the continuous retraction of glaciers, the soil starts to present a gradient of physical, chemical, and biological factors reflecting regional changes over time. Little is known about the biological nature of fungi in Antarctic glacier soils. In this sense, this work aimed at studying the behavior of fungal community structure from samples of glacier soil collected after glacial retreat (Collins Glacier). A total of 309 fungi distributed in 19 genera were obtained from eleven soil samples. Representatives of the genera Pseudogymnoascus (Ascomycota) and Mortierella (Mortierellomycota) were the most abundant isolates in all samples. The data revealed the presence of filamentous fungi belonging to the phylum Basidiomycota, rarely found in Antarctica. Analysis of the generalized linear models revealed that the distance from the glacier as well as phosphorus and clay were able to modify the distribution of fungal species. Environmental variations proved to have influenced the genera Pseudogymnoascus and Pseudeutorium.

Use of MALDI-TOF MS for fungal species distribution of interdigital intertrigo in seafarers, Dakar, Senegal

To determine fungal species distribution of interdigital intertrigo among seafarers in Dakar, Senegal, a cross-sectional study was carried out from May to August 2017 among seafarers clinically diagnosed with interdigital intertrigo. A questionnaire was filled to each patient before sampling the affected folds and transporting to Aristide Le Dantec University Hospital where mycological analyses were realized. Species identification by MALDI-TOF MS was performed in Marseille, France. In total, 169 men (21–66 years) were included. Few of them (3%) had a high level of education and the duration of the mycosis exceed 10 years for 88% of patients. Direct microscopic examination (ME) was positive in 34.3%. Among samples with positive ME, 58.6% had positive culture. An overall incidence of 30.2% was found. Patients with confirmed cases aged between 28 and 66 years. Among them, those between 36–50 years were predominant (52.9%). Those with a high level of education were less representative (2%). For 52.1% of patients, the duration of the mycosis was superior to 10 years. Furthermore, 57% of cases were significantly associated with other types of tinea pedis and/or onychomycosis (P=0.03). Culture was positive in 23.7% isolating 43 strains successfully identified at the species level by MALDI-TOF MS for 31 isolates: 20 Candida and 11 dermatophytes. The rest was identified only at the genus level belonged to Fusarium. In definitive, MALDI-TOF MS could be a useful tool for routine and fast identification of dermatophytes, yeasts and NDFF in clinical mycology laboratories.

Analysis of the pathogenic characteristics of fungal bloodstream infection in severe burn patients

Objective: To retrospectively analyze the diagnosis time, pathogen distribution, and drug resistance of fungal bloodstream infection in severe burn patients. Methods: Blood samples were collected from 55 severe burn patients with fungal bloodstream infection (including 46 males and 9 females, aged 42 (1, 78) years) admitted to the intensive care unit of the Institute of Burn Research of the First Affiliated Hospital of Army Medical University (the Third Military Medical University) from July 2011 to May 2019 for retrospective analysis. Microbial monitoring system was used to cultivate pathogens, API yeast identification kit and Candida chromogenic medium were used to identify pathogens, and Kirby-Bauer paper disk diffusion method was used to detect drug resistance of fungi to fluconazole, amphotericin B, itraconazole, ketoconazole, and voriconazole. The positive rate of blood fungal culture, mortality rate, distribution of local fungal proliferation sites, the diagnosis time distribution of fungal bloodstream infection, the distribution of fungal species, resistance to commonly-used antifungal drugs, and the use of antibiotics were assessed. The WHONET 5.6 software was applied to analyze the distribution and drug resistance of fungi. Results: (1) Totally 4 839 blood samples were collected during the 9 years, and 122 strains of fungi were isolated, with positive rate of 2.52%. The mortality rate was 14.55% (8 patients) in 55 patients. Catheter fungal proliferation ranked the first among 30 cases of local fungal proliferation. (2) The diagnosis time of fungal bloodstream infection mainly distributed in ≤1 week of hospitalization [32.73% (18/55)]. (3) Among the 55 strains of fungi detected, the detection rate of Candida parapsilosis ranked the first (21.82%, 12 strains), Candida glabrata was the second (18.18%, 10 strains), and Candida tropicalis was tied with Candida albicans in the third place (14.55%, 8 strains). All the detected fungi were sensitive to amphotericin B, and the resistance rates to voriconazole, fluconazole, itraconazole, and ketoconazole were between 4.5% and 9.1%. (4) Droad-spectrum antibiotics were used in all the 55 patients, ≥3 kinds of antibiotics were used in 44 patients, and 37 patients used antibacterial drugs ≥7 days. Conclusions: The diagnosis time of fungal bloodstream infection in the 55 severe burn patients was mainly within 1 week of hospitalization. Candida parapsilosis is the most commonly detected fungal species. Catheter fungal proliferation occurs most commonly among the 30 patients with local fungal proliferation. All the detected fungi were sensitive to amphotericin B, with low drug resistance to voriconazole, fluconazole, itraconazole, and ketoconazole. Broad-spectrum antibiotics were overused in the severe burn patients with fungal bloodstream infection.

Determination of Soil Properties and Fungal Interrelations Influenced by Forest Restoration on a Contaminated Zinc Mining Area

Forest restoration in a contaminated zinc mining area must take into account the environmental soil properties. This study aimed to analyze the soil properties from the three different stages of restored forests, at 3, 7 and 17, focusing on the impact on soil microorganisms and availability of fungal species. Three study sites were selected, all located in a zinc mining area in Mae Sot District, Tak Province, Thailand. The results showed that observed soil properties especially soil texture, moisture and nutrients differed between stand initiation and regrowth forests and affected the distribution of fungal species. Soils in older forest stands up to 17 years old showed higher sand content and reduced soil moisture, SOM, and certain nutrients. Forest restoration was found to favour development of a range of soil properties, some of which were suitable for fruiting in nine fungal species from the following families: Agaricaceae, Psathyrellaceae, Cantharellaceae, Phallacae, Coprinaceae, Sclerodermataceae, Lycoperdaceae, Marasmiaceae, and Clavariaceae.

Mucormycosis experience through the eyes of the laboratory

Background: Mucormycosis is a rare, worldwide fungal infection with high mortality, which mostly affects immunocompromised patients. Compared to large parts of Asia, Europe, and the USA, information on clinical expression and fungal species distribution in mucormycosis in Turkey is limited.Objectives and methods: The main aim of this study was to evaluate the demographic features of mucormycosis cases, identify the isolates at the species level by using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF), compare culture results with histopathological examination and determine the antifungal susceptibility patterns of the pathogens.Results: Between 2016 and 2018, 10 mucormycosis cases (six female, four male; age range: 35–74 years) were evaluated retrospectively. The predominance of the cases were in late autumn and winter. Diabetes mellitus was the most common underlying condition. Seven patients had rhinoorbitocerebral, two had pulmonary and one had cutaneous mucormycosis. By mycological culture and direct microscopic examination nine strains were identified as Rhizopus spp. and one as Mucor spp. Seven of these strains were identified at the species level by MALDI-TOF. Histopathological examination of eight tissues were reported as compatible with mucormycosis. All isolates were resistant to azoles and echinocandins. Two isolates were resistant to Amphotericin B and one was resistant to posaconazole. Surgical debridement combined with antifungal therapy was the main treatment option. The mortality rate was 40% (n = 4) and the mean number of days between the onset of complaints and the initiation of treatment was 9.25.Conclusions: Early, rapid and accurate diagnosis of mucormycosis is of critical importance in the treatment of immunosuppressed patients.