Background: Otomycosis (fungal otitis externa), is a fungal infection of external auditory canal (EAC). It is a common challenging problem in the ENT clinic, because for the need for long term treatment and follow up. The present study was thus designed with the view to know the mycological pattern encountered in otomycosis along with the aetio-pathological factors and its management. Methodology: A prospective study was done in the Department of ENT, Sri Siddhartha Medical College & Hospital, Tumkur for a period of 12 months, total of 51 patients of both genders with clinical features of otomycosis were enrolled in the study. 2 swabs were taken from the EAC of all cases and after aural toileting under microscope, were treated with 1%clotrimazole and followed up for 2 months Results: A total of 51 patients were examined in this study, of which 47 cases produced positive fungal isolates (92.1%). Females were more commonly affected than males. Most common pre-disposing factor was manipulation of the ear by unsterile material for cleaning the ear. Itching and pain were the most common symptoms, Aspergillus was the most common fungus isolated. Clotrimazole showed good response symptomatically and mycological clearance was seen within a week. Conclusion: Otomycosis is still a common problem and is often misdiagnosed for other chronic otitic conditions. Fungi thrive on exfoliated dead epithelial cells along with humid environment in EAC. Thorough cleaning of fungi followed by topical antifungal agent is important in management of this condition. Clotrimazole is effective, safe and well tolerated topical antifungal agent.