BACKGROUND: Gait disorders are common among 80% of stroke patients. Its consequences include increased risk of falls and functional limitations, which can significantly reduce the quality of life. AIM: To investigate the functional profile of hemiparesis resulting from subacute ischemic stroke. MATERIALS AND METHODS: This observational, retrospective, one-stage, single-center study analyzed primary biomechanical gait parameters in 31 patients and 34 healthy controls. Spatial, temporal, kinematic, and electromyographic characteristics were recorded. A statistical assessment and inter-group and intra-group comparison of the collected data were performed to identify the pathognomonic features of hemiplegic gait in patients with subacute stroke. RESULTS: Changes in gait biomechanics typical for hemiparetic patients who have suffered from ischemic stroke were described: slight asymmetry of step cycle, normal duration of support period on the paretic side, significant increase in duration of support period on the healthy side, and shortening of single support period on the paretic side. Additionally, the asymmetry of the walking function was characterized by changes in reciprocity, that is, a harmonious sequence of step cycles. The function of the hip and knee joints was reduced and altered, and the amplitude of the ankle joint was increased. Decrease and change in the bioelectric activity profile of muscles were detected. Moreover, the quadriceps femoris function was least affected. CONCLUSION: The main changes in walking function are characterized by an asymmetry of spatiotemporal parameters, a decrease in movement amplitudes of the hip and knee joints on the paretic side, an increase in ankle joint amplitude, a decrease in the bioelectric activity of muscles, and a shift in the phases of their activity. The results contribute to a better understanding of the mechanisms behind hemiplegic gait and provide a valuable tool for developing more personalized and targeted rehabilitation plans for patients suffering from hemiparesis as a result of subacute ischemic stroke.