Functional Activity Of Leukocytes Research Articles

The estimation of intensity of inflammatory alterations of blood in early postinfarction remodeling

Abstract Funding Acknowledgements Type of funding sources: None. Introduction Structural postinfarction abnormalities is connected with inflammation. For better understanding of pathogenesis of postinfarction remodeling, it was studied the intensity of inflammatory disorders in early postinfarction period. Purpose Research of the intensity of inflammatory response in early postinfarction remodeling. Materials and methods 772 of patients with myocardial infarction (MI) were examined. Clinical blood analysis was performed at admission and on the 5th day of MI, as well as immunological examination, Echocardiography. Results In patients with end-diastolic diameter (EDD) of left ventricle (LV) ≥ 55 mm, 5 day MI white blood cells (WBC) level (8,0 ±2,1*109/l vs 7,3 ± 1,9*109/l, p = 0,0001), absolute number of neutrophils (5,0 ± 1,7* 109/l vs 4,5 ± 1,4*109/l, p = 0,0006) and monocytes (0,64 ± 0,30*109/l vs 0,57 ± 0,30*109/l, p = 0,005) were higher compared patients with EDD ≤ 55 mm. The inflammatory alterations in patients with increased end-diastolic volume (EDV) and end-systolic volume (ESV) of left ventricle was similar. The LV EDD was correlated with WBC level (r = 0,3; p = 0,00002), also with absolute number of neutrophils (r = 0,3; p = 0,0002) and monocytes (r = 0,3; p = 0,007). The positive connection of EDV LF with level of WBC (r = 0,3; P = 0,007) and absolute number of neutrophils (r = 0,3; p = 0,0002) was revealed. The positive connection of ESV LV with absolute number of neutrophils (r = 0,3, р=0,04; accordingly). It should be noted that increase of ESD LV more 37mm was not accompanied by authentic increase of inflammatory factors, but the connection of ESV LV with Il-6 was noted (ρ=0,3; р=0,03). As far as contractive function of myocardium declined, it was registered the increase of neutrophils (neutrophils on the 5 day in EF 55% and more - 4,5 ± 1,4*109/l, EF 55-40% - 4,8 ± 1,5*109/l, EF less 40% - 5,1 ± 2,1*109/l, p = 0,002), also in formation of areas of akinesia (neutrophils on the 1 days - 9,2 ± 3,3*109/l vs 8,3 ± 3,1*109/l, р=0,05) and aneurysm of LV (5,08 ± 1,71*109/l vs 4,52 ± 1,48*109/l, р=0,0006). It should be noticed that functional activity of monocytes in patients with aneurism of LV was lower than in patients without aneurism (CD14 0,035 ± 0,02*109/l vs 0,049 ± 0,02*109/l, р=0,05). It appears important obtained data that with hypertrophy LV and diastolic disfunction was noticed authentic increase of lymphocytes, expressing the marker of apoptosis CD95 (0,458 ± 0,276*109/l vs 0,335 ± 0,155*109/l, р=0,05; 0,441 ± 0,26*109/l vs 0,342 ± 0,21*109/л, р=0,01, accordingly). Conclusion The increased activity of inflammatory markers, decline of functional activity of monocytes, the elevation of apoptosis marker, associated with bigger intensity of postinfarction remodeling processes. The acute phase of inflammation is necessary stage of healing, but with long-term clinical case, excessive activation or alteration of functional activity of leukocytes can lead to growth of structural and electrical remodeling.

Interrelation of the content of transferrin, erythrocytes with the functional activity of leukocytes of peripheral venous blood in residents of the European North of the Russian Federation

Interrelation of the content of transferrin, erythrocytes with the functional activity of leukocytes of peripheral venous blood in residents of the European North of the Russian Federation

Orthopaedic Surgery Elicits a Systemic Anti-Inflammatory Signature.

Little information is available on the functional activity of leukocytes after arthroplasty or the expansion of populations with immune suppressive properties during the acute post-operative period. Synovial fluid and matched pre- and post-surgical blood samples were collected from total hip and knee arthroplasty patients (THA and TKA, respectively) to examine the impact of surgery on peripheral blood leukocyte frequency, bactericidal activity, and inflammatory mediator expression. For spinal surgeries, inflammatory mediator production by peripheral blood mononuclear cells (PBMCs) pre- and post-surgery was examined. An expansion of immune suppressive granulocytic myeloid-derived suppressor cells (G-MDSCs) was observed following arthroplasty, which correlated with significantly increased serum interleukin-10 (IL-10) levels. Analysis of synovial fluid from THA and TKAs revealed reduced granulocyte colony-stimulating factor (G-CSF) and soluble CD40 ligand (sCD40L) and increased interleukin-6 (IL-6), monocyte chemoattractant protein 2 (CCL2) and Fms-like tyrosine kinase 3 ligand (Flt-3L) compared to pre- and post-surgical serum. For the spinal surgery cohort, stimulation of PBMCs isolated post-surgery with bacterial antigens produced significantly less pro-inflammatory (IL-1α, IL-1β, interleukin-1 receptor antagonist (IL-1RA), IL-12p40, growth-related oncogene-α/GRO-α (CXCL1) and 6Ckine (CCL21)) and more anti-inflammatory/tissue repair mediators (IL-10, G-CSF and granulocyte-macrophage colony-stimulating factor (GM-CSF)) compared to PBMCs recovered before surgery. The observed bias towards systemic anti-inflammatory changes without concomitant increases in pro-inflammatory responses may influence susceptibility to infection following orthopaedic surgery in the context of underlying co-morbidities or risk factors.

CHANGES IN THE DYNAMICS OF THE FUNCTIONAL ACTIVITY OF BLOOD GRANULOCYTES IN PATIENTS WITH PANCREATIC NECROSIS.

The purpose of research. Predicting risk of infection of acute destructive pancreatitis.
 Material and Methods. The dynamics of functional activity of granulocytes in 27 (58,7%) patients with sterile and 19 (41,3%) patients with infected pancreatic necrosis. An objective assessment of the severity of their condition carried out by performed using of the scale of the integrated assessment APPACHE-II. The exclusion criterion from the study was the revealing of accompanying acute and chronic diseases.
 Results.Scale APPACHE-II can be used mainly for screening assessment of the degree of polyorgan insufficiency, as well as predicting the development of infected pancreatic necrosis forms, but, unfortunately, only in the later stages of development of pathological process. Dynamics of changes in the functional activity of leukocytes in patients with pancreatic necrosis can be used as an additional test, which characterizes the moment of transition of sterile pancreatic necrosis to infected, and, consequently, in deciding on the timing of terms and nature of performance of operational allowance.
 Conclusions. 1. In patients with sterile pancreatic necrosis (SPN) after a moderate decrease in the activity of myeloperoxidase (MPO), the content of the cation proteins (CP) in the first three days in subsequent periods of observation, on the background of complex conservative therapy, noted, compared with the established "Normal" gradual increase or stabilization at close to the indicators of the functional activity of neutrophils. The activity of KF and alkaline phosphatase from patients with SPN, after the increase in performance in the first 11 days, then gradually declines, reaching the normal level. 2. Patients with infected pancreatic necrosis(IPN) sharply, starting with 11 days of the study, by 35-40%, reduce the activity of MPO and the content of CB and Acid Phosphatase activity and Alkaline Phosphatase, on the contrary, it had progressively pronounced growth after 11 days by 35-40%.

Peculiarities of leukocyte apoptosis modulation in children with pyelonephritis

One of the leading places among inflammatory diseases of the urinary tract of children belongs to pyelonephritis, the course of which presents in most cases as a severe infectious disease threatening the patient’s life, which is the main reason for development of chronic kidney failure. This study was conducted to compare apoptosis stages in peripheral blood of children of different age categories with pyelonephritis depending on etiological factor and complications. The problem of mechanisms underlying immune system misregulation, especially functional activity of leukocytes in children with pyelonephritis, have not been explored in recent years. Assessment of leukocytes (neutrophils) apoptosis stages in peripheral blood of children of different age categories with pyelonephritis depending on complications and etiological factor was the aim of present study. The children's peripheral blood samples were analysed and assessed using a flow cytofluorimeter. The present study demonstrates an increase of the level of apoptotic cells at an early stage of apoptosis in children of all age categories with chronic pyelonephritis, which can be explained by associations of a wide range of pathogens and the presence of sequelae. An increase in the number of apoptotic cells in the late stage of apoptosis is observed in children aged 1 month – 8 years, in children 8–18 years, the amount of apoptotic cells is reduced by 1.5 times. The study of apoptosis stages allows complete characterization of the dynamics of the apoptotic process and supplementation of the pathogenesis of pyelonephritis in children. Such studies will make it possible to affect apoptosis modulation to regulate or correct it and encourage the finding of innovative solutions in the treatment related to influence on the immune response. We conclude that enhancement of peripheral blood leukocyte apoptosis in chronic form of pyelonephritis especially in young children is due to the polyetiology of this form of pyelonephritis and the development of complications.

Agmatine Prevents Oxidative-nitrative Stress in Blood Leukocytes Under Streptozotocin-induced Diabetes Mellitus.

Changes in cellular metabolism, development of oxidative-nitrative stress and intensification of glycation and lipid peroxidation (LPO), are significant processes that occur during diabetes mellitus (DM)-associated chronic hyperglycemia. These processes contribute to deviations in the structural organization and functional activity of leukocytes. The development of oxidative-nitrative stress in peripheral blood cells during DM can be prevented by agmatine, an endogenous metabolite of L-arginine, which is a nitric oxide synthase (NOS) inhibitor, and possesses hypoglycemic properties. The administration of agmatine to animals with DM lead to the inhibition of both constitutive and inducible NOS in leukocytes, which in turn decreased total nitrite/nitrate (NOx) levels. Additionally, we observed corresponding increases in reduced glutathione content and activity of antioxidant enzymes (SOD, CAT, GPx, GR), along with decreased levels of the thiobarbituric acid reactive substance, advanced oxidation protein products (AOPPs) and advanced glycosylation end-products (AGEs) as compared to the non-treated diabetic group. Our results indicate that treatment of diabetic animals with agmatine restores redox homeostasis and a balances antioxidant defence system enzymes in leukocytes. This corrective effect on the functional capacity of leukocytes is exerted by preventing oxidative-nitrative stress in animals with DM.

Влияние окислительного стресса на функциональную активность лейкоцитов и тромбоцитов у больных коксиеллезом

Влияние окислительного стресса на функциональную активность лейкоцитов и тромбоцитов у больных коксиеллезом

BRAIN BLOOD CIRCULATION DISORDERS AND ACTIVITY OF LEUKOCYTES

Every year about 6 million people in the world have a stroke. Our purpose is to study cases of brain blood circulation disorders in order to determine functional activity of leukocytes of the peripheral blood in patients with chronic ischemia and brain infarction. To carry out the comparative analysis of indicators of functional activity of leukocytes of blood with the clinical picture of a disease and indicators of the hemostasis system. 100 persons (60 men and 40 women at the age of 18 and older) who had a stroke within 1-3 weeks were examined. Tomography (MRI) in the examination of brain of primary patients with the clinical diagnosis of a stroke has been conducted. Sharp brain blood circulation disorder was confirmed by means of MRI at 35 of 100 patients. Examination of 31 patients’ brain was conducted between 3 and 12 hours after the beginning of the disease. The comparison group was made of 30 healthy persons (30%). In a group of patients with the chronic cerebral ischemia (CCI) there is an increase in quantity of leukocytes (by 1.3 times). In a group of patients with an ischemic stroke insignificant decrease in quantity of leukocytes with activated chromatin below the control indicators is noted. Degranulation of leukocytes takes place, as well as the emission of the lysosomal enzymes into the pericellular space. This confirms the participation of cells of a lymphoid line of peripheral blood in sharp cerebral ischemia. Distinct correlation between functional activity of blood leukocytes and a clinical picture of a disease and hemostasis system indicators was revealed. This can serve as a method of differential diagnostics of patients with chronic ischemia and brain infarction, as well as an objective way of controlling the efficiency.

Anti-inflammatory activity of pectins and their galacturonan backbone

It has been shown that pectin polysaccharides from different plants, depending on their structure, can either protect the intestinal walls of mammals against damage and inhibit the development of inflammation or, on the contrary, have proinflammatory effects. At the same time, galacturonan isolated from any pectin, being the main carbohydrate chain (backbone) of its macromolecule, shows a marked anti-inflammatory effect. A decrease in the quantity of neutrophiles in the intestinal wall after induced inflammation indicates that the anti-inflammatory effects of pectins can be based on their influence on the functional activity of leukocytes.

Effect of photosensitizers pheophorbide a and protoporphyrin IX on skin wound healing upon low-intensity laser irradiation

The effect of photosensitizer with subsequent He-Ne (632.8 nm; 3 mW/cm2) laser irradiation on experimental skin wound healing has been studied. Pheophorbide a and protoporphyrin IX were used as photosensitizers. It was found that application of the photosensitizer and subsequent laser irradiation, first, decreased the amount and the functional activity of leukocytes in the wound exudate and, second, inhibited the SOD activity as compared with that of the control group. Moreover, pheophorbide and protoporphyrin practically did not affect the total healing period but decreased the length of the inflammation stage. It was supposed that these effects are related to generation of reactive oxygen species during irradiation.

A study on oxidative stress and complete blood count of sheep bred in the area exposed to depleted uranium (DU) ammunition

The paper presents the results of several health status parameters of sheep bred in the area exposed to depleted uranium (DU) ammunition during NATO bombing of Serbia and Montenegro in 1999. The blood samples of sheep were collected randomly in the region of Bujanovac, in 2004. Complete blood count was performed according to standard laboratory procedures. Concentration of red blood cells malondialdehyde (RBC MDA) and activity of erythrocyte superoxid dismutase (SOD) were determined spectrophotometrically, while the functional activity of leukocytes was investigated by NBT reduction and adhesion test. The results of complete red blood cells count indicated normocytic hypochromic anaemia. The total number of leukocytes and the differential leukocytes count were within the physiological range. Although the leukocytes adhesiveness was not changed in DU exposed animals, their increased NBT reduction revealed stimulated leukocytes' oxidative burst. This finding, together with significantly increased RBC MDA concentrations, as well as the activity of RBC antioxidant SOD, point to the existence of oxidative stress. Although the results indicated that animals were under oxidative stress, still there are no conclusive data that it was due to the exposure of DU that entered the environment during military operations.

The effect of low-intensity laser irradiation in the blue, green, and red spectral ranges on healing of experimental skin wounds in rats

The effects of low-intensity laser irradiation in the red (632.8 nm), green (532 nm), and blue (441.2 nm) spectral ranges on wound healing has been studied in rats. The effect of the traditionally used red laser irradiation has been compared with the effect caused by laser irradiation in other spectral ranges, aiming to support the provisional hypothesis that a similar healing effect could be achieved at lower doses of wound irradiation by lasers emitting in the blue and green spectral ranges. The following parameters have been used to characterize healing of the experimental wounds: the functional activity of phagocytes in the wound exudate, which was determined from luminol-dependent chemiluminescence, the phagocyte number; the wound exudates’ antioxidant activity; and the rate of healing, which was determined as the change of the wound surface area. It was found that in all cases the laser irradiation accelerated the healing of wounds. Exposure to red laser irradiation at the dose of 1.5 J/cm2), and to blue or green laser irradiation at a dose of 0.75 J/cm2 shortened the time of the wound healing from 22 to 17 and 19 days, respectively. The functional activity of leukocytes in irradiated groups increased by day 5 after surgery, whereas in the control group it decreased. The superoxide dismutase activity increased in all experimental groups by day 5 after surgery. Laser irradiation in the red spectral range at a dose of 1.5 J/cm2 resulted in a larger increase in superoxide dismutase activity, as compared to that found after exposure to laser irradiation in the blue and green spectral ranges at a dose of 0.75 J/cm2.

A study on oxidative stress and peripheral blood parameters of cows bred in the area exposed to depleted uranium ammunition

The paper presents results of the study on depleted uranium (DU) health effects on cows bred in areas exposed to DU ammunition, during NATO bombing of Serbia and Montenegro in 1999. The samples of animal blood, soils and feed were collected randomly in the region of Bujanovac, in 2003. Complete blood cells count was performed according to standard laboratory procedures. Concentration of red blood cells malondialdehyde (RBC MDA) and erythrocyte superoxid dismutase (SOD) activity were determined spectrophotometrically. The functional activity of leukocytes was investigated by NBT reduction and adhesion test. Activity of the radionuclides was determined by standard gamma spectrometry. The obtained results of complete red blood cells count indicate normocytic normochromic anaemia. Considerably increased RBC MDA concentration suggests a violent oxidative stress in cows bred in the exposed area although the activity of their RBC antioxidant enzyme SOD was in the range of values obtained in the control group. The total number of leukocytes and the differential leukocyte counts were within the physiological range. NBT reduction test revealed the absence of leukocyte oxidative burst, which pointed to the absence of inflammation. A significant decrease of granulocyte adhesiveness, pointed at partial impairment of granulocyte functional activity. The activity of 238U/235U in soils and feed was below the minimal detectable concentration (10-3 Bq/kg), while the content of natural (226Ra and 232Th) and anthropogenic 137Cs) radionuclides in soils were within the average values for the region, except for low levels of 40K - indicating soils impoverishment in potassium.

Transforming factor-bl and markers of leukocytic activation in hypertensive disease

The study was undertaken to determine the serum concentration of an active form of transforming growth factor-bl (TGF-bl) and the functional status of leukocytes in patients with Stage II hypertensive disease (HD) and to assess the pathogenetic value and capacities of correction of detected changes with the angiotensin-converting enzyme (ACE) inhibitor quinapril. Materials and methods. Thirty patients with Stage II HD and 17 apparently healthy individuals were examined. The data of ultrasound studyofthe heart, radial and carotid arteries, the functional activity of leukocytes and the concentration of (TGF-bl) in the serum were assessed. Of them, 15 patients with HD were reexamined during treatment with quinapril (accupro, Pfizer, USA) in a daily dose of 10-40 mg for 12 weeks. Results. The study established an increase in the elevated serum levels of TGF-bl of hypertensive patients with HD and its association with the mass of the left ventricular myocardium, with the thickness of an intima-media complex of the common carotid arteries, and with endothelial dysfunction. A relationship is shown between the concentration of TGF-bl and the functional activity of leukocytes: elevated peripheral leukocytes, the adhesive capacity of neutrophils, the lymphocytic expression of the antiapoptic protein Bcl-2 and Fas-receptors. With quinapril treatment, a 10-mm Hg or more reduction in 87% of the patients was attended by the lower adhesion of neutrophils to the endothelium, with elevated Bcl-2-negative and Fas-positive lymphocytes. Conclusion. The changes in the serum levels of TGF-bl are associated with lesions of target organs and with the activation of leukocytes in HD. The decrease in leukocytic functional activity during therapy with quinapril did not depend on the antihypertensive effect of the drug.

Effect of fibrinogen on functional activity of blood leukocytes.

Fibrinogen intensified luminol-dependent chemiluminescence of blood leukocytes stimulated with opsonized zymosan. It is hypothesized that fibrinogen stimulates and prolongs functional activity of leukocytes during inflammation.

Blood picture as an indicator of the impact of low-intensity factors on the body of workers in the production of xylene

Exposure to xylene, at low concentrations (less than 50 mg / m3) under the conditions of its production, from petroleum raw materials was characterized in the first 3 years of work by the development of hematopoietic irritation in working people, as well as an increase in the functional activity of leukocytes. 5 years after the start of work under these conditions, the above indicators approached the data of the control group, and then there was a decrease in the functional usefulness of leukocytes.

Skin-window study on the migration of leukocytes of newborns and infants

Migration of leukocytes of newborns and of infants up to the age of 6 months was studied using the in vivo skin-window technique according to Rebuck. Using the non-specific stimulation (abrasion of the skin only) a slight age-dependent physiological increase of migration of cells was observed within the observation period; after a strong local irritation with diphtheria-tetanus-pertussis vaccine (Alditepera) there was a vigorous migratory response of cells in the skin lesion. Both quantitatively and qualitatively, the migration pattern (i.e. shift from PMN leukocyte to mononuclear cells in the exudate within a 1 d period after abrasion) was not influenced by immunization of infants with Alditepera, suggesting thus the nonspecific nature of this cellular response. The "normal" values of the chemotactic response of leukocytes of newborns and infants are given as a basis for evaluation of defects of this functional activity of leukocytes.