Function Of Soil Microorganisms Research Articles

Genomic fingerprints of the world's soil ecosystems.

Despite the explosion of soil metagenomic data, we lack a synthesized understanding of patterns in the distribution and functions of soil microorganisms. These patterns are critical to predictions of soil microbiome responses to climate change and resulting feedbacks that regulate greenhouse gas release from soils. To address this gap, we assay 1,512 manually curated soil metagenomes using complementary annotation databases, read-based taxonomy, and machine learning to extract multidimensional genomic fingerprints of global soil microbiomes. Our objective is to uncover novel biogeographical patterns of soil microbiomes across environmental factors and ecological biomes with high molecular resolution. We reveal shifts in the potential for (i) microbial nutrient acquisition across pH gradients; (ii) stress-, transport-, and redox-based processes across changes in soil bulk density; and (iii) greenhouse gas emissions across biomes. We also use an unsupervised approach to reveal a collection of soils with distinct genomic signatures, characterized by coordinated changes in soil organic carbon, nitrogen, and cation exchange capacity and in bulk density and clay content that may ultimately reflect soil environments with high microbial activity. Genomic fingerprints for these soils highlight the importance of resource scavenging, plant-microbe interactions, fungi, and heterotrophic metabolisms. Across all analyses, we observed phylogenetic coherence in soil microbiomes-more closely related microorganisms tended to move congruently in response to soil factors. Collectively, the genomic fingerprints uncovered here present a basis for global patterns in the microbial mechanisms underlying soil biogeochemistry and help beget tractable microbial reaction networks for incorporation into process-based models of soil carbon and nutrient cycling.IMPORTANCEWe address a critical gap in our understanding of soil microorganisms and their functions, which have a profound impact on our environment. We analyzed 1,512 global soils with advanced analytics to create detailed genetic profiles (fingerprints) of soil microbiomes. Our work reveals novel patterns in how microorganisms are distributed across different soil environments. For instance, we discovered shifts in microbial potential to acquire nutrients in relation to soil acidity, as well as changes in stress responses and potential greenhouse gas emissions linked to soil structure. We also identified soils with putative high activity that had unique genomic characteristics surrounding resource acquisition, plant-microbe interactions, and fungal activity. Finally, we observed that closely related microorganisms tend to respond in similar ways to changes in their surroundings. Our work is a significant step toward comprehending the intricate world of soil microorganisms and its role in the global climate.

Understanding and exploring the diversity of soil microorganisms in tea (Camellia sinensis) gardens: toward sustainable tea production.

Leaves of Camellia sinensis plants are used to produce tea, one of the most consumed beverages worldwide, containing a wide variety of bioactive compounds that help to promote human health. Tea cultivation is economically important, and its sustainable production can have significant consequences in providing agricultural opportunities and lowering extreme poverty. Soil parameters are well known to affect the quality of the resultant leaves and consequently, the understanding of the diversity and functions of soil microorganisms in tea gardens will provide insight to harnessing soil microbial communities to improve tea yield and quality. Current analyses indicate that tea garden soils possess a rich composition of diverse microorganisms (bacteria and fungi) of which the bacterial Proteobacteria, Actinobacteria, Acidobacteria, Firmicutes and Chloroflexi and fungal Ascomycota, Basidiomycota, Glomeromycota are the prominent groups. When optimized, these microbes' function in keeping garden soil ecosystems balanced by acting on nutrient cycling processes, biofertilizers, biocontrol of pests and pathogens, and bioremediation of persistent organic chemicals. Here, we summarize research on the activities of (tea garden) soil microorganisms as biofertilizers, biological control agents and as bioremediators to improve soil health and consequently, tea yield and quality, focusing mainly on bacterial and fungal members. Recent advances in molecular techniques that characterize the diverse microorganisms in tea gardens are examined. In terms of viruses there is a paucity of information regarding any beneficial functions of soil viruses in tea gardens, although in some instances insect pathogenic viruses have been used to control tea pests. The potential of soil microorganisms is reported here, as well as recent techniques used to study microbial diversity and their genetic manipulation, aimed at improving the yield and quality of tea plants for sustainable production.

Floristic changes and environmental drivers of soil fungi and archaea in different salt-tolerant plant communities in the intertidal habitat of coastal wetlands.

Microorganisms are crucial elements of terrestrial ecosystems, which play significant roles in improving soil physicochemical properties, providing plant growth nutrients, degrading toxic and harmful chemicals, and biogeochemical cycling. Variations in the types and quantities of root exudates among different plants greatly alter soil physicochemical properties and result in variations in the diversity, structure, and function of soil microorganisms. Not much is understood about the differences of soil fungi and archaea communities for different plant communities in coastal wetlands, and their response mechanisms to environmental changes. In this study, fungal and archaea communities in soils of Suaeda salsa, Phragmites australis, and Spartina alterniflora in the intertidal habitat of coastal wetlands were selected for research. Soil fungi and archaea were analyzed for diversity, community structure, and function using high throughput ITS and 16S rRNA gene sequencing. The study revealed significant differences in fungi and archaea's diversity and community structure in the rhizosphere soil of three plant communities. At the same time, there is no significant difference in the functional groups. SOM, TP, AP, MC, EC and SOM, TN, TP, AP, MC, EC are the primary environmental determinants affecting changes in soil fungal and archaeal communities, respectively. Variations in the diversity, community structure, and ecological functions of fungi and archaea can be used as indicators characterizing the impact of external disturbances on the soil environment, providing a theoretical foundation for the effective utilization of soil microbial resources, thereby achieving the goal of environmental protection and health promotion.

Combined effects of azoxystrobin and oxytetracycline on rhizosphere microbiota of Arabidopsis thaliana

The rhizosphere is one of the key determinants of plant health and productivity. Mixtures of pesticides are commonly used in intensified agriculture. However, the combined mechanisms underlying their impacts on soil microbiota remain unknown. The present study revealed that the rhizosphere microbiota was more sensitive to azoxystrobin and oxytetracycline, two commonly used pesticides, than was the microbiota present in bulk soil. Moreover, the rhizosphere microbiota enhanced network complexity and stability and increased carbohydrate metabolism and xenobiotic biodegradation as well as the expression of metabolic genes involved in defence against pesticide stress. Co-exposure to azoxystrobin and oxytetracycline had antagonistic effects on Arabidopsis thaliana growth and soil microbial variation by recruiting organic-degrading bacteria and regulating ABC transporters to reduce pesticide uptake. Our study explored the composition and function of soil microorganisms through amplicon sequencing and metagenomic approaches, providing comprehensive insights into the synergistic effect of plants and rhizosphere microbiota on pesticides and contributing to our understanding of the ecological risks associated with pesticide use.

Metagenomic analysis reveals the effect of revegetation types on the function of soil microorganisms in carbon and nitrogen metabolism in the open-cast mining area

Metagenomic analysis reveals the effect of revegetation types on the function of soil microorganisms in carbon and nitrogen metabolism in the open-cast mining area

Soil microbial responses to multipollutant exposures in megacity's parks of Beijing

Soil life revolves around microorganisms that are crucial for soil ecosystems and health. In megacities, the combined exposure of multiple pollutants exerts a significant impact on the structures and functions of soil microorganisms; however, there is a lack of empirical studies on this topic. Hence, we conducted a study including urban parks in Beijing, China. The results indicate that bacteria were abundant in the soils of Beijing parks, showing the same dominant groups but different rare groups. The dominant groups included Actinobacteria and Proteobacteria. Candidate phyla radiation bacteria, a large evolutionary radiation of bacterial lineages whose members remain mostly uncultivated, were the main specialists. Under the combined exposure of multiple pollutants, the structures of soil microbial communities in different parks were similar. Community change due to pollutants (31%) was greater than that due to natural factors (2.4%). Among multipollutants, organophosphate esters, led by dibutyl phosphate, had the highest influence on microbial abundance and distribution. An increase in dibutyl phosphate concentration decreased the abundance of Firmicutes, while the abundance of Synergistota was increased. The interactions among pollutants affecting the bacteria were different. Bis(2-chloroethyl) phosphate, nickel and benzo[g,h,i]perylene influenced microorganisms by working with organophosphate esters. High-molecular-weight polycyclic aromatic hydrocarbons, such as benzo[a]pyrene and benzo[g,h,i]perylene, mainly acted on the functional genes and thus affected multiple biogeochemical cycles. Benzo[a]anthracene, bis(2-chloroethyl) phosphate, and arsenic were the primary pollutants affecting metabolic pathways. Our research helps to better understand the impacts of urban environmental pollution on soil microorganisms.

Soil Microbial Community and Their Relationship with Soil Properties across Various Landscapes in the Mu Us Desert

Soil microorganisms play crucial roles in maintaining material circulation and energy flow in desert ecosystems. However, the structure and function of soil microorganisms in different forestlands are currently unclear, restricting the use of sand-fixing plants and the understanding of forest ecosystem functions. In this study, Artemisia ordosica, Caragana korshinskii, and Salix psammophila, three types of sand-fixing forests widely distributed in the Mu Us Sandy Land, were used to explore the effects of sand-fixing forests on soil physicochemical properties, soil enzyme activity, soil microbial biomass, microbial community structure, and inter-microbial species relationships. Soils of forestlands showed higher soil organic carbon (SOC), total phosphorus (TP), and total nitrogen (TN) contents than bare sandy land. The SOC in bare sandy soil was only 0.84 g kg−1, while it remained 1.55–3.46 g kg−1 in forestland soils. The TN in bare sandy land soil was 0.07 g kg−1, which was significantly lower than that in forestland soils (0.35–0.51 g kg−1). The TP in bare sandy soil was 0.18 g kg−1, significantly lower than that in forestland soils (0.46–0.69 g kg−1). Afforestation of bare sandy land improved soil microbial carbon and nitrogen contents and increased microbial enzyme activities of acid phosphatase and N-acetyl-β-D-glucosaminidase. Significant differences were observed between the three forestlands and bare sandy land in terms of soil microorganisms and community composition. With the establishment of a sand-fixing forest, the alpha diversity of soil bacteria significantly improved, whereas that of soil fungi remained stable. The bacterial community comprised 33 phyla, 106 classes, 273 orders, 453 families, and 842 genera. While five fungal phyla were detected by OTUs at a similarity of 97%, bacterial and fungal community structures were affected by the organic carbon content, sand particle content, soil pH, total nitrogen, and total phosphorus contents of soils. This study is helpful for vegetation construction and protection on sandy lands from the perspective of plant-microbe interactions.

Effects of the polyhalogenated carbazoles 3-bromocarbazole and 1,3,6,8-tetrabromocarbazole on soil microbial communities

Soil ecosystems are being more contaminated with polyhalogenated carbazoles (PHCZs), which raising much attention about their impact on soil microorganisms. 3-Bromocarbazole (3-BCZ) and 1,3,6,8-tetrabromocarbazole (1,3,6,8-TBCZ) are two typical PHCZs with high detection rates in the soil environment. However, ecological risk research on these two PHCZs in soil is still lacking. In the present study, after 80 days of exposure, the ecological influence of 3-BCZ and 1,3,6,8-TBCZ was investigated based on 16S rDNA sequencing, ITS sequencing, gene (16S rDNA, ITS, amoA, nifH, narG and cbbL) abundance and soil enzyme activity. The results showed that the bacterial 16S rDNA gene abundance significantly decreased under 3-BCZ and 1,3,6,8-TBCZ exposure after 80 days of incubation. The fungal ITS gene abundance significantly decreased under 1,3,6,8-TBCZ (10 mg/kg) exposure. PHCZs contributed to the alteration of bacteria and fungi community abundance. Bacteria Sphingomonas, RB41 and fungus Mortierella, Cercophora were identified as the most dominant genera. The two PHCZs consistently decreased the relative abundance of Sphingomonas, Lysobacter, Dokdonella, Mortierella and Cercophora etc at 80th day. These keystone taxa are related to the degradation of organic compounds, carbon metabolism, and nitrogen metabolism and may thus have influence on soil ecological functions. Bacterial and fungal functions were estimated using functional annotation of prokaryotic taxa (FAPROTAX) and fungi functional guild (FUNGuild), respectively. The nitrogen and carbon metabolism pathway were affected by 3-BCZ and 1,3,6,8-TBCZ. The soil nitrogen-related functions of aerobic ammonia oxidation were decreased but the soil carbon-related functions of methanol oxidation, fermentation, and hydrocarbon degradation were increased at 80th day. The effects of 3-BCZ and 1,3,6,8-TBCZ on the abundances of the amoA, nifH, narG, and cbbL genes showed a negative trend. These results elucidate the ecological effects of PHCZs and extend our knowledge on the structure and function of soil microorganisms in PHCZ-contaminated ecosystems.

The influence and risk assessment of multiple pollutants on the bacterial and archaeal communities in agricultural lands with different climates and soil properties

Soil microorganisms play a vital role in maintaining the health of farmland ecosystems. The structures and functions of soil microorganisms are determined by the combined effects of natural and anthropogenic processes. To investigate the influence of anthropogenic contaminants on agricultural soil conditions and their impact on the prokaryote community, we collected agricultural soils from 3 types of villages (industrial, livestock breeding and agricultural) in four regions with typical geoclimatic features. We assessed the influences of changing environmental factors (climatic conditions, soil properties, heavy metal and organic pollutants) on soil bacterial and archaeal communities and their relative contributions. The results showed that differences in soil properties and contaminants between regions were more significant than those between villages of different types. Cu, Zn, Cd, antibiotics and phthalate esters were identified as major pollutants with medium to high level of risk in agricultural soils. The richness and biodiversity index showed no significant differences among regions, while dominant species and community composition were significantly correlated with changing environmental and pollution factors. Major environmental factors explained 65 % of the total variances based on the RDA analysis, with average temperature, precipitation and soil pH identified as the major factors shaping the prokaryote community. Combined pollution explained 20.14 % of total community variances, with moderate levels of pollution stresses being as significant as climatic and soil conditions to community composition. The correlation analysis indicated that different species respond differently to environmental factors. The major groups of functional microbes for N cycling, Nitrososphaeraceae and Nitrospiraceae, were found to be stressed by combined pollution. The Bacillaceae family showed a negative correlation to soil pH and a positive correlation to pollutants, suggesting their resistance to changing environments. The interaction among species and the correlation between environmental factors and multiple pollutants brought significant complexity to the assessment of co-contamination. In general, this study provides insights into the responses of agricultural soil microbial communities to multiple changing factors at a continental scale, which would substantially benefit the soil health assessment and management of agro-ecosystems under global environmental changes.

Effects of soil habitat changes on antibiotic resistance genes and related microbiomes in paddy fields

The changes of paddy soil habitat profoundly affect the structure and function of soil microorganisms, but how this process drives the growth and spread of manure- derived antibiotic resistance genes (ARGs) after entering the soil is unclear. Herein, this study explored the environmental fate and behavior of various ARGs in the paddy soil during rice growth period. Results showed that most ARG abundances in flooded soil was lower than that in non-flooded soil during rice growth (decreased by 33.4 %). And soil dry-wet alternation altered microbial community structure in paddy field (P < 0.05), showing that Actinobacteria and Firmicutes increased in proportion under non-flooded conditions, and Chloroflexi, Proteobacteria and Acidobacteria evolved into the dominant groups in flooded soil. Meanwhile, the correlation between ARGs and bacterial communities was stronger than that with mobile genetic elements (MGEs) in both flooded and non-flooded paddy soils. Furthermore, soil properties, especially oxidation reduction potential (ORP), were proved to be an essential factor in regulating the variability of ARGs in the whole rice growth stage by structural equation model, with a direct influence (λ = 0.38, P < 0.05), following by similar effects of bacterial communities and MGEs (λ = 0.36, P < 0.05; λ = 0.29, P < 0.05). This study demonstrated that soil dry-wet alternation effectively reduced the proliferation and dissemination of most ARGs in paddy fields, providing a novel agronomic measure for pollution control of antibiotic resistance in farmland ecosystem.

Understory vegetation diversity, soil properties and microbial community response to different thinning intensities in Cryptomeria japonica var. sinensis plantations.

Soil microorganisms are the key factors in elucidating the effects of thinning on tree growth performance, but the effects of vegetation and soil on the species composition and function of soil microorganisms after thinning are still not well elaborated. The effects of thinning on understory vegetation diversity, soil physicochemical properties and soil microbial community composition were investigated in a thinning trial plantation of Cryptomeria japonica var. sinensis, including four thinning intensities (control: 0%, LIT: 20%, MIT: 30% and HIT: 40%), and the relationships of the microbial community structure with the understory vegetation diversity and soil properties were assessed. The results showed that thinning had a greater effect on the diversity of the shrub layer than the herb layer. The soil bulk density and the contents of soil organic matter, total potassium and nitrogen increased with increasing thinning intensities. The Shannon and Chao indices of soil bacteria and fungi were significantly lower in the LIT, MIT and HIT treatments than in the control. Thinning can significantly increase the abundance of Proteobacteria and Actinobacteria, and higher thinning intensities led to a higher relative abundance of Ascomycota and a lower relative abundance of Basidiomycota, Rozellomycota, and Mortierellomycota. Redundancy analysis indicated that soil physicochemical properties rather than understory vegetation diversity were the main drivers of microbial communities, and fungi were more sensitive to soil properties than bacteria. Functional prediction showed that thinning significantly reduced the potential risk of human diseases and plant pathogens, and the nitrogen fixation capacity of bacteria was the highest in the HIT treatment. Thinning significantly increased the relative abundance of cellulolysis and soil saprotrophs in bacteria and fungi. The findings provide important insights into the effects of thinning on C. japonica var. sinensis plantation ecosystems, which is essential for developing thinning strategies to promote their ecological and economic benefits.

Diversity and function of soil microorganisms in response to paddy–upland rotation system in sustainable restoration of saline-sodic soils

Context Rapid desalination by planting rice in saline soil consumes large amounts of water, which is not environmentally friendly. Aims Herein, we propose rapid desalination by planting rice, then shifting to cultivating upland plants to attain substantial resource-saving and higher yield simultaneously with restoration of saline-sodic soil. Methods Field experiments were run for two consecutive years with five treatments: unreclaimed wasteland (WL) as control, rice cultivation followed by fallow (RF), rice–rice continuous cropping (RR), rice–ryegrass rotation (RG), and rice–sorghum rotation (RS). Physicochemical properties, including pH, electrical conductivity, and exchangeable sodium percentage were determined, and 16S rRNA sequences were used to evaluate soil microbial composition and stability. Key results The soil total organic carbon, total nitrogen, available phosphorus, and biomass in RR, RG, and RS treatments were all higher than RF and control. Notably, RR, RG, and RS increased the soil microbial biomass carbon and nitrogen, and significantly reshaped the soil communities of bacteria, fungi, and archaea relative to RF and WL. Conclusions Despite the lower efficiency of RG and RS in ameliorating saline-sodic soil, there were dramatic savings in irrigation water, and the improvements in microbial diversity and functionalities indicated that the paddy–upland crop rotation system had substantial influence on sustainability of soil quality. Implications Providing a balance between salt desalination performance with irrigation water input and yield, the paddy–upland rotation system is a robust, replicable, and environmentally friendly practice in saline-sodic soil remediation.

Alternate wetting–drying enhances soil nitrogen availability by altering organic nitrogen partitioning in rice-microbe system

Alternate wetting and drying (AWD) irrigation influences soil nutrient cycling and the functioning of soil microorganisms. However, the effects of AWD on organic nitrogen (ON) partitioning in rice-microbe ecosystems and soil microbial communities are poorly understood. A root-box microcosm experiment with two rice varieties (Nipponbare, Nip; Yangdao 6, YD6), two irrigation regimes (conventional flood irrigation (CF); AWD) and three N application levels (zero N, N0; medium N, N1; high N, N2) was performed based on the 13C,15N-labelled glycine and 13C-phospholipid fatty acids (PLFA) techniques. Compared to CF, AWD increased soil dissolved oxygen, microbial growth and the enzymes related to N transformation, thus enhancing rice growth and the N utilization index (NUI). Approximately 4.9–10.3% and 7.7–13.6% of the exogenous glycine was directly utilized by Nip and YD6 seedlings, respectively, and its ratio increased with increasing N levels, whereas 4.4–11.2% and 4.6–10.3% were incorporated into soil microbes. It seems that rice has an appreciable capacity to utilize organic N despite fierce competition with soil microbes. The 13C:15N ratio showed that 12.5–37.5% and 11.0–41.0% of the added glycine was taken up intact by soil microorganisms in the rhizospheres of Nip and YD6. At the N1 and N2 levels, AWD increased rice 15N-glycine uptake but decreased microbial 15N-glycine uptake. Rice intact glycine uptake and soil inorganic N contents were positively correlated with rice biomass and NUI, indicating that the enhanced inorganic N under AWD is beneficial for soil ON availability and rice growth. Gram-negative and general bacteria were the dominant competitors with rice for soil ON. AWD increased the contents of gram-negative bacteria and fungi and the ratio of fungi:bacteria in rhizosphere soils. Collectively, AWD creates a good rhizosphere oxygen environment for rice and microbial growth. ON partitioning in the rice-microbe system and the adaptive adjustment of the microbial community play important roles in modulating soil nutrients for optimal rice growth.

Abundance, Diversity, and Function of Soil Microorganisms in Temperate Alley-Cropping Agroforestry Systems: A Review.

Modern temperate alley-cropping systems combine rows of trees with rows of crops (agroforestry), which allows for diverse interspecific interactions such as the complementary and competitive use of resources. The complementary use of resources between trees and crops is considered the main advantage of these multifunctional land use systems over cropland monocultures. Moreover, several studies demonstrated that agroforestry systems are environmentally more sustainable than cropland monocultures. Over two decades of research on soil microorganisms in temperate alley-cropping systems are characterized by a variety of different methodological approaches and study designs to investigate the impact of agroforestry on the soil microbiome. Here, we review the available literature on the abundance, diversity, and functionality of soil microorganisms in temperate alley-cropping systems. Further, we identify current knowledge gaps as well as important experimental factors to consider in future studies. Overall, we found that temperate alley-cropping systems increase soil microbial abundance, diversity, and functions as compared to cropland monocultures, which is expected to contribute to enhanced biological soil fertility in these systems.

Microplastics as an Emerging Environmental Pollutant in Agricultural Soils: Effects on Ecosystems and Human Health

Microplastics are &amp;lt;5 mm in size, made up of diverse chemical components, and come from multiple sources. Due to extensive use and unreasonable disposal of plastics, microplastics have become a global environmental issue and have aroused widespread concern about their potential ecological risks. This review introduces the sources, distribution and migration of microplastics in agricultural soil ecosystems. The effects of microplastics on soil physicochemical properties and nutrient cycling are also discussed. Microplastics can alter a series of key soil biogeochemical processes by changing their characteristics, resulting in multiple effects on the activities and functions of soil microorganisms. The effects of microplastics on soil animals and plants, the combined effects of microplastics and coexisting pollutants (organic pollutants and heavy metals), and their potential risks to human health are also discussed. Finally, prevention and control strategies of microplastic pollution in agricultural soil ecosystems are put forward, and knowledge gaps and future research suggestions about microplastic pollution are given. This review improves the understanding of environmental behavior of microplastics in agricultural soil ecosystems, and provides a theoretical reference for a better assessment of the ecological and environmental risks of microplastics.

Soil organic matter, nitrogen and pH driven change in bacterial community following forest conversion

Fast expansion of forest conversion to monoculture plantations has profound effects on ecosystem structure and functions. Through altering litter and rhizodeposition composition as well as one-sided effects on soil chemical properties, monoculture plantations may tremendously decrease biodiversity and functions of soil microorganisms. We investigated the impacts of conversion of natural evergreen and deciduous broad-leaf forest (Forest) to four 5-year old monocultureplantations (plantations), such as Camellia oleiferaAbel.(Oil), Amygdalus persica (Peach), Myrica rubra (Lour.) S. et Zucc.(Berry) and Cunninghamia lanceolata (Lamb.) Hook.(Fir), on soil properties and bacterial community and its driving factors using the high-throughput sequencing technique. Soil organic carbon, and total nitrogen decreased up to 59%–83% and pH increased by 0.31 units following the forest conversion. The changes in soil properties and bacterial communities were depended on the type of the plantation. Bacterial diversity increased by 6.5% after forest conversion. The relative abundances of Acidobacteria, Proteobacteria, Bacteroidetes and Actinobacteria in the Forest were remarkably higher compared with all plantations, whereas that of Chloroflexi and Planctomycetes was less. All plantations had low abundance of Acidobacteria, while the highest abundance of Proteobacteria, Actinobacteria and Planctomycetes was observed under Peach. The co-occurrence patterns of bacterial communities identified rare taxa rather than abundant taxa as central players in bacterial network. The redundancy analysis indicated that the variation in the composition of bacterial community was mainly driven by soil pH, organic carbon and total nitrogen content. Therefore, good management practices, such as reasonable fertilization and soil erosion prevention, need to be developed for monoculture plantations to mitigate the depletion of nutrients and to enhance microbial functioning after forest conversion.

Urban Grassland Management Implications for Soil C and N Dynamics: A Microbial Perspective

Urban grasslands are turfgrass dominated landscapes of varying functions and uses that are ubiquitous in areas associated with human population growth and urbanization. While these landscapes are perceived to serve a primarily aesthetic function, they provide a multitude of beneficial ecosystem functions that impervious surfaces do not provide. Urban grassland soils have been shown to accumulate carbon (C) and nitrogen (N) for decades, matching native grasslands and eastern hardwood forest soils in terms of C and N densities. The establishment and maintenance of urban grasslands alters many microbially-mediated biogeochemical processes in soils, including soil organic matter (SOM) dynamics. Despite strong evidence of alterations to soil C and N cycling, the impacts of maintaining urban grasslands on soil microbiomes and their functions remain understudied compared to other ecosystems. Typical management practices can directly and indirectly affect edaphic factors in urban grasslands, which in turn, could impact soil processes mediated by microorganisms. We reviewed the existing literature on urban grassland management, focusing on how mowing, fertilization, irrigation, grass species composition, and soil cultivation could impact the composition and function of soil microorganisms. Although sparse, the literature indicates that the techniques used to maintain urban grassland habitats broadly select for copiotrophic microorganisms adapted to higher resource availability. Additionally, the studies indicate that greater soil fertility and plant productivity found in urban grasslands facilitate the accumulation of soil C and N, as well as SOM as compared to other land-use types. However, effects on soil biology and biogeochemistry depend on specific management practices, which are quite variable. Future research on soil C and N dynamics in urban grasslands should focus on the dominant component of this ecosystem—residential lawns—however, much of the existing scientific literature featuring turfgrass systems focus heavily on golf courses, athletic fields, and major tourist parks.

Effects of long-term fertilization and water addition on soil properties and plant community characteristics in a semiarid grassland.

We summarized the effects of fertilization and water addition on some soil properties and plant community characteristics in a long-term field experiment established in 2005 in a degraded grassland in Duolun, Inner Mongolia, China. The results showed that nitrogen (N) addition resulted in surface soil acidification and decreased acid buffering capacity, increased the availability of carbon (C), N, phosphorus (P), sulfur (S) and DTPA-extractable iron (Fe), manganese (Mn), and copper (Cu) contents, depleted the sum of base cations calcium (Ca), magnesium (Mg), potassium (K) and sodium (Na), decreased the diversity of soil microbial community. Nitrogen addition enhanced the uptake of N, P, S, K, Mn, Cu and Zn by plants, while inhibited plant Fe uptake, but with no effect on the uptake of Ca or Mg. Nitrogen addition increased aboveground net primary productivity (ANPP) but declined plant species diversity and community stability. Phosphorus addition alone increased total P and Olsen-P contents and fungal abundance in the surface soil, and improved N, P and S uptake by leaves, but had no significant influence on other soil basic chemical properties, ANPP, and plant species diversity. Water addition could improve the resistance of plant community, but its contribution to ANPP was limited by soil N availa-bility. Water addition could buffer soil acidification and the decline of microbial and plant diversity induced by N addition. Under the treatments of N and water addition or P and water addition, the diversity and function of soil microorganisms were affected by plant community structure and function. Long-term controlled field experiments were useful for understanding ecosystem structure and functions of grasslands. However, to uncover the underlying mechanisms in grassland ecosystem ecology, single-site experiments should be incorporated with multiple-site controlled field experiments in different regions. More attentions should be paid to the linkage of above- and below-ground ecological processes.

Study on the effect mechanism of Arbuscular Mycorrhiza on the absorption of heavy metal elements in soil by plants

In recent years, human activities have produced lots of wastes, including a large number of heavy metals, which have entered the natural ecosystem, changing the activity and function of soil microorganisms. AM fungi can adapt to or resist metal stress, which means it has great utilization potentiality in promoting plant growth, enhancing nutrient acquisition and for phytoremediation in heavy metal contaminated soil. On the basis of previous research results, the leading mechanism of metal tolerance of AM fungi, the influencing factors of mycorrhizal effect and the application in promoting the process of phytoremediation are discussed in this paper, which provides theoretical basises for improving plant tolerance and enhancing mycorrhizal strengthening effect under heavy metal pollution.

Effects of grassland afforestation on structure and function of soil bacterial and fungal communities

Grassland afforestation strongly influences the structure and function of soil microorganisms. Yet the mechanisms of how afforestation could simultaneously alter both the soil fungal and bacterial communities and its implications for ecosystem management are poorly understood, especially in nitrogen-limited ecosystems. Using high-throughput sequencing of 16S rRNA and ITS rRNA genes, the present study investigated the changes in soil properties and soil microorganisms after afforestation of natural grasslands with Chinese pine (Pinus tabuliformis) on the Loess Plateau in China. Results showed that soil bacterial diversity had no significant differences among the grassland (GL), forest–grassland transition zone (TZ), and forestland (FL), while soil fungal diversity in the GL was significantly higher than that in the FL and TZ (P < 0.05). The proportion of shared OTUs in the soil bacterial community was higher than that in the soil fungal community among the three land use types. The dominant bacterial phylum shifted from Proteobacteria to Actinobacteria, while the dominant fungal phylum shifted from Ascomycota to Basidiomycota after the GL conversion to the FL. The functional groups of ECM fungi increased significantly while biotrophic fungi decreased significantly after grassland afforestation. Both the soil bacterial and fungal communities in the TZ showed great similarity with those in the FL. In addition, among all examined soil properties, soil nitrogen (N) showed a more significant effect on the soil microbial communities. The reduction of soil N after grassland afforestation resulted in both the structure and function changes in soil microbial communities. Our results demonstrated simultaneously differential changes in the composition and diversity of both soil bacterial and fungal communities after afforestation from grasslands to planted forests.