Growth Function Research Articles

An investigation of firm size distributions involving the growth functions

Following the ideas of prospect theory, a class of growth functions is used to characterize deterministic variations of firm size, which portrays the asymmetric efforts of the firm to achieve the desired size. Considering the differences in the ability of different firm size to cope with uncertainties, the Boltzmann equation for the evolution of firm size is constructed. Utilizing a suitable scaling limit, the Fokker–Planck equation is acquired and its explicit steady-state solution is derived. Our results illustrate that different choices of parameters in the growth function lead to various statistical laws for firm size, such as the Amoroso distribution, the lognormal distribution and Zipf’s law. Under certain conditions, inequality for the distribution of firm size decreases as firm size increases. The numerical analyses are presented to illustrate our results.

Analyses of the bHLH gene family in Populus trichocarpa reveal roles of four PtbHLHs in regulating the drought stress response

As one of the largest families of transcription factors in plants, the basic helix-loop-helix (bHLH) transcription factor family regulates a wide range of functions in plants. However, little is known about the functions of bHLH family members in Populus trichocarpa during plant growth and in the response to drought stress. In our study, 190 PtbHLH genes were identified in the P. trichocarpa genome and classified into 21 groups. Analyses of microarray datasets showed that most PtbHLH members not only have multiple functions in poplar growth, but also respond rapidly to drought stress in the leaves or roots. We selected four genes, PtbHLH35, PtbHLH121, PtbHLH137, and PtbHLH152, which were highly expressed in leaves or roots under drought stress, for functional validation analyses. These genes encoded nucleus-localized bHLH transcription factors. Transient expression of PtbHLH35, PtbHLH121, and PtbHLH152 in P. trichocarpa improved drought tolerance by activating the antioxidant system to eliminate reactive oxygen species and reduce the degree of cell damage in the leaves under drought stress. Overexpression of PtbHLH137 improved drought tolerance by activating antioxidant enzymes in the roots to eliminate reactive oxygen species, and by increasing the abscisic acid content in the roots in response to drought stress. Together, our findings provide insights into the functions of PtbHLH family members in growth and in the response to drought.

Biogenic volatile organic compounds (BVOCs) emissions and physiological changes in Pinus densiflora and Quercus acutissima seedlings under elevated particulate matter (PM)

Urban trees effectively reduce air pollution, including particulate matter (PM), which is a major concern in East Asia. While acting as biofilters, urban trees can be affected by PM exposure, which hinders their growth and physiological functions, thereby reducing their pollution mitigation ability. Trees absorb pollutants but also emit biogenic volatile organic compounds (BVOCs), which can act as precursors to other forms of air pollution. To better understand the effects of PM on urban trees, this study examined how two tree species, Pinus densiflora and Quercus acutissima, respond to elevated PM levels under controlled conditions at a concentration of 300 μg m−3. The aim was to investigate how increased PM levels affect BVOCs emissions and physiological responses in seedlings, and how these physiological changes influence BVOCs emission pattern. The results revealed species-specific responses in BVOCs emissions under PM stress with being especially oxygenated monoterpenes more than non‑oxygenated monoterpenes. Increased PM adsorption was found to reduce photosynthetic abilities, including photosynthesis (Anet), carboxylation capacity (Vcmax), and electron transport rate (J). This reduction in photosynthetic efficiency was further evidenced by decreased chlorophyll content and light absorption, which were assessed through chlorophyll fluorescence measurements. Additionally, the study evaluated oxidative stress indicators, such as lipid peroxidation and the accumulation of reactive oxygen species (ROS), to provide a comprehensive understanding of the species' responses to elevated PM conditions. The study found that elevated PM conditions were closely linked to an increase in oxygenated monoterpenes, which was associated with both oxidative stress and impaired physiological function. These observations emphasize the need for strategic urban tree selection to enhance air quality and suggest that understanding species-specific BVOCs emissions in response to PM is crucial for optimizing urban green spaces for health and environmental benefits.

Association of Serum Ferritin With Growth and Endocrine Function in Thalassemia Major Children in North India: An Observational Study

Association of Serum Ferritin With Growth and Endocrine Function in Thalassemia Major Children in North India: An Observational Study

Investigating the Mechanism of Neurotoxic Effects of PFAS in Differentiated Neuronal Cells through Transcriptomics and Lipidomics Analysis.

Per- and polyfluorinated alkyl substances (PFAS) are pervasive environmental contaminants that bioaccumulate in tissues and pose risks to human health. Increasing evidence links PFAS to neurodegenerative and behavioral disorders, yet the underlying mechanisms of their effects on neuronal function remain largely unexplored. In this study, we utilized SH-SY5Y neuroblastoma cells, differentiated into neuronal-like cells, to investigate the impact of six PFAS compounds─perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), perfluorodecanoic acid (PFDA), perfluorodecanesulfonic acid (PFDS), 8:2 fluorotelomer sulfonate (8:2 FTS), and 8:2 fluorotelomer alcohol (8:2 FTOH)─on neuronal health. Following a 30 μM exposure for 24 h, PFAS accumulation ranged from 40-6500 ng/mg of protein. Transcriptomic analysis revealed 721 differentially expressed genes (DEGs) across treatments (padj < 0.05), with 11 DEGs shared among all PFAS exposures, indicating potential biomarkers for neuronal PFAS toxicity. PFOA-treated cells showed downregulation of genes involved in synaptic growth and neural function, while PFOS, PFDS, 8:2 FTS, and 8:2 FTOH exposures resulted in the upregulation of genes related to hypoxia response and amino acid metabolism. Lipidomic profiling further demonstrated significant increases in fatty acid levels with PFDA, PFDS, and 8:2 FTS and depletion of triacylglycerols with 8:2 FTOH treatments. These findings suggest that the neurotoxic effects of PFAS are structurally dependent, offering insights into the molecular processes that may drive PFAS-induced neuronal dysfunction.

Deletion of RBM20 exon 9 impairs skeletal muscle growth and satellite cell function in pigs

Maintaining healthy skeletal tissue is essential for overall well-being and quality of life. Skeletal muscle plays a key role in this process, yet models for studying its detailed function are limited. While RNA-binding motif protein 20 (RBM20) is primarily associated with dilated cardiomyopathy (DCM), its role in skeletal muscle remains largely unexplored. This study investigates RBM20 function in skeletal muscle using an RBM20 exon 9 deletion pig model (RBM20E9D). The deletion of exon 9 resulted in loosely arranged muscle fibers, large inter-fiber gaps, and irregular organization, leading to impaired muscle growth and development. Analysis of skeletal muscle satellite cells revealed significantly reduced proliferation, diminished myotube formation in vitro, and disrupted sarcomere structure due to exon 9 deletion. Given the critical role of satellite cell proliferation and differentiation in muscle repair, RBM20E9D pigs offer a novel model for studying the mechanisms underlying skeletal muscle injury, repair, and growth.

Impact of severe drought on the age structure of a population of African savannah elephants

Impact of severe drought on the age structure of a population of African savannah elephants

Yes, they're all individuals: Hierarchical models for repeat survey data improve estimates of tree growth and size

Abstract Repeat measurement surveys of tree size are used in forests to estimate growth behaviour, biomass and population dynamics. Although size is measured with error and individuals vary in their growth trajectories, current size‐based growth modelling approaches do not usually or fully account for both of these features, and therefore under‐utilise available data. We present a new method that leverages the auto‐correlation structure of repeat surveys into a hierarchical Bayesian longitudinal growth model. This new structure allows users to correct for measurement error and capture individual‐level variation in growth trajectories and parameters. To demonstrate the new method we applied it to a sample of tropical tree survey data from long‐term monitoring sites at Barro Colorado Island. We were able to reduce estimated error in size and growth, and extract individual‐ and population‐level growth parameter estimates. We used simulation to evaluate the ability of the new method to improve estimates of growth rate and size, and estimate individual and species‐level parameters. Our method substantially improved the root mean squared error (RMSE) for growth by an average of 61% compared to existing approaches using pairwise differences and reduced RMSE in estimated size RMSE compared to ‘observed’ values in simulated data. Better numerical integration methods (Runge–Kutta fourth order in comparison to Euler and midpoint) provided better estimates of parameters, but did not improve the estimation of size and growth. The choice of a positive growth function eliminated all negative increments without data exclusion. Overall, this study shows how we can gain new and improved insights on growth, using repeat forest surveys. Our new method offers improved biomass dynamics estimation through reduced error in sizes over time, coupled with novel information about within‐species variation in growth behaviour that is inaccessible with species average models, such as individual parameters for the growth function which allows for relationships between parameters to be considered for the first time.

On Bergman-Orlicz and Campanato-Orlicz spaces of hyperbolic harmonic functions on the real unit ball

Let Φ be a growth function. In this paper, we characterize Bergman-Orlicz spaces B α Φ of hyperbolic harmonic functions on the real unit ball B in terms of invariant gradients and some integral functions. As an application, several characterizations of h-harmonic Campanato-Orlicz spaces are established. This is a continuous work of [X. Fu and Q. Shi. Complex Var. Elliptic Equ. 2023].

Effects of selenium on the growth and immune gene functions of red swamp crayfish (Procambarus clakii)

Effects of selenium on the growth and immune gene functions of red swamp crayfish (Procambarus clakii)

Efficacy of Lactobacillus acidophilus and yeast cell wall-derived supplements on immunity responses, growth performance, and disease resistance in Cyprinus carpio juveniles.

This study investigated the effects of probiotic Lactobacillus acidophilus (PTCC 1643) (LA) and a commercial yeast cell wall prebiotic (Immunogen®) (IM) on immunoantioxidant, growth, and digestive status, and resistance to Aeromonas hydrophila in Cyprinus carpio juveniles. The fish were fed in five treatments including control (T1), LA1.5 (T2): 1.5 (× 10^8 CFU/g of LA)/kg diet, LA3 (T3): 3 (× 10^8 CFU/g of LA)/kg diet, IM1 (T4): 1g of IM/kg diet, and IM2 (T5): 2g of IM/ kg diet. After 60 days, the function of growth and intestinal protease and amylase showed a significant increase in IM treatments. Serum levels of total protein and superoxide dismutase in LA3 treatment, as well as albumin, catalase, and glutathione peroxidase in LA3 and IM2 treatments were significantly increased. Serum level of malondialdehyde in LA and IM treatments, as well as alkaline phosphatase (ALP) in LA treatments, alanine aminotransferase in LA and IM2 treatments, and lactate dehydrogenase in LA3 treatment was significantly decreased. Serum levels of all the immune parameters in LA3 treatment, as well as alternative complement pathway hemolytic and nitroblue tetrazolium in IM2 treatment were significantly improved. Mucosal level of protease in LA3 and IM2 treatments, total immunoglobulin, and lysozyme in LA and IM2 treatments, as well as ALP and peroxidase in LA3 treatment was significantly increased. Cumulative mortality rate in LA and IM treatments was significantly decreased compared to control after the 14-day challenge with A. hydrophila. It is recommended to include IM2 in the diet of C. carpio juveniles with regard to its beneficial effects on growth and immunity status.

Lamiaceae family-derived endophytic fungi: induced tolerance to drought stress in Thymus vulgaris plants

BackgroundThymus vulgaris is a valuable medicinal plant widely cultivated for its aromatic and medicinal properties. However, like many plants, T. vulgaris faces challenges such as drought stress, which significantly affects its growth, morphological, physiological, and biochemical processes. Understanding how endophytic fungi isolated from Lamiaceae family influence T. vulgaris under varying watering regimes can enhance its resilience against drought stress. This study aims to assess the impact of individual and co-inoculation of three native endophytic species, i.e., Fusarium sp. (F1), Cladosporium puyae (F2), and Curvularia australiensis (F3), on T. vulgaris growth parameters under different irrigation regimes in greenhouse conditions.ResultsIt has been discovered that using fungal endophytes as a biological tool can benefits T. vulgaris under drought stress. The results indicated that drought stress significantly reduced the growth, chlorophyll, and carotenoid content of plants lacking endophytes. Combinatory applications with fungal endophytes significantly improved the above-mentioned parameters under drought stress. Lipid peroxidation levels were significantly reduced in plants inoculated with bacterial endophytes. Drought stress significantly increased the activities of ascorbate peroxidase (APX), superoxide dismutase (SOD), glutathione reductase (GR), peroxidase (POD), and catalase (CAT) in drought conditions.ConclusionsThe findings suggested that the addition of fungal endophytes to the inoculum enhances drought tolerance in T. vulgaris by mitigating the harmful impact of drought stress on plant growth and physiological functions. The higher activity of antioxidant enzymes and improved redox state of glutathione are responsible for plants’ greater resistance to drought.

Muscle type-specific effects of bilateral abobotulinumtoxinA injection on muscle growth and contractile function in spastic mice.

Intramuscular injection of botulinum neurotoxin type A (BoNT-A) is commonly used to improve or maintain the joint range of motion in young children with spasticity. However, the effectiveness of BoNT-A treatment is variable and movement limitations are recurrent. Here we show long-term effects of a single, bilateral abobotulinumtoxinA (aboBoNT-A) injection in the gastrocnemius medialis and soleus muscles of wild-type and spastic (B6.Cg-Glrbspa/J with a mutation in the glycine receptor) mice at a young age (6-7 days). Specifically, we evaluated the impact of aboBoNT-A-A on gait, physical performance, and spontaneous physical behavior, as well as on contractile force characteristics, morphology, and histological phenotype of soleus and gastrocnemius muscles by comparing their results to those of saline-injected controls up to 9 weeks after the injection. The detailed time course of the study specifies the timing of the aboBoNT-A injection at 1 week, the period of behavioral studies from 4-9 weeks, and the age of the mice (10 weeks) at the time of contractile force characteristics and histology assessments. In spastic mice, aboBoNT-A injection had a minor and very specific effect on physical performance, by only modestly increasing stride length as a function of age. aboBoNT-A injection caused a reduction in the force-generating capacity and a slightly smaller physiological cross-sectional area in gastrocnemius medialis, but not in soleus. Reduced physiological cross-sectional area in aboBoNT-A-injected muscles was due to a lower number of muscle fibers, rather than reduced muscle fiber cross-sectional area. The percentage of slow-type muscle fibers and mitochondrial succinate dehydrogenase activity were increased, which was associated with an improved muscle endurance capacity. In conclusion, aboBoNT-A injection reduced the number of muscle fibers, causing muscle hypertrophy in remaining fibers and a shift towards more oxidative fibers, resulting in an improved endurance capacity and gait. This study proposed potential cellular mechanisms for the therapeutic efficacy of aboBoNT-A in spasticity.

Temperature and feeding frequency: interactions with growth, immune response, and water quality in juvenile Nile tilapia

BackgroundWater temperature and feeding frequency are critical abiotic factors regulating the growth and immune function of aquatic organisms. This study investigated the effects of water temperature and feeding frequency on growth and immune function in Nile tilapia (Oreochromis niloticus) over two months. A total of 360 juvenile fish (average weight: 20.00 ± 1.26 g) were divided into six groups, each with three replicates, based on a combination of three water temperatures (26, 28, and 30 °C) and two feeding frequencies (either 1 or 2 meals per day).ResultsAt 30 ºC and 28 ºC, water electrical conductivity and total dissolved salts increased, while total ammonia nitrogen and dissolved oxygen rose slightly in groups fed twice daily, with a significant interaction between temperature and feeding frequency. The group at 30 ºC with two meals per day showed the highest final body weight (FBW). The interaction between temperature and feeding frequency significantly influenced FBW, total feed intake, and body thickness. Fish at 30 ºC exhibited upregulated hepatic growth hormone receptor 1 and insulin-like growth factor 1, while those at 28 ºC with one meal per day, as well as those at 30 ºC regardless of meal frequency, also showed increased expression of hepatic fatty acid binding protein and intestinal cluster of differentiation 36. Fish at 30 ºC had upregulated leptin levels and downregulated cholecystokinin, while those at 26 ºC displayed the opposite trend, particularly with one meal daily. Higher temperatures significantly boosted serum IgM, superoxide dismutase (SOD), and lysozyme (LYZ) levels, with meal frequency also affecting malondialdehyde, IgM, and SOD levels. Additionally, 30 ºC enhanced the hepatic expression of mucin-like protein (muc), oligo-peptide transporter 1 (pept1), interleukin 1, nf-κB, complement C3, lyz, sod, catalase, and glutathione peroxidase, with twice-daily meals having a more pronounced effect. Conversely, 28 ºC with one meal per day upregulated some of these genes, such as muc, pept1, and sod.ConclusionsOverall, 30 ºC with two meals per day significantly improved the growth and health of juvenile Nile tilapia, while 28 ºC with two meals maintained satisfactory performance.

The relationships between cochlear nerve health and AzBio sentence scores in quiet and noise in postlingually deafened adult cochlear implant users.

This study investigated the relationships between the cochlear nerve (CN) health and sentence-level speech perception outcomes measured in quiet and noise in postlingually deafened adult cochlear implant (CI) users. Study participants included 24 postlingually deafened adult CI users with a Cochlear® Nucleus™ device. For each participant, only one ear was tested. Neural health of the CN was assessed at three or four electrode locations across the electrode array using two parameters derived from results of the electrically evoked compound action potential (eCAP). One parameter was the phase locking value (PLV) which estimated neural synchrony in the CN. The other parameter was the sensitivity of the eCAP amplitude growth function (AGF) slope to changes in the interphase gap (IPG) of biphasic electrical pulses (i.e., the IPGE slope ). Speech perception was tested using AzBio sentences in both quiet and a ten-talker babble background noise with +5 dB and +10 dB signal-to-noise ratios (SNR). IPGE slope and PLV values were averaged across electrodes for each subject, both with and without weighting by the frequency importance function (FIF) of the AzBio sentences. Pearson and Spearman correlations were used to assess the pairwise relationships between the IPGE slope , the PLV, and age. Multiple linear regression models with AzBio score as the outcome and the PLV and the IPGE slope as predictors were used to evaluate the associations between the three variables while controlling for age. The correlation between the IPGE slope and the PLV was negligible and not statistically significant. The PLV, but not the IPGE slope , differed significantly across electrodes, where the apical electrodes had larger PLVs (better neural synchrony) than the basal electrodes. The IPGE slope , but not the PLV, was significantly correlated with participant's age, where smaller IPGE slope values (poorer CN health) were associated with more advanced age. The PLV, but not the IPGE slope , was significantly associated with AzBio scores in noise, where larger PLVs predicted better speech perception in noise. Neither the PLV nor the IPGE slope was significantly associated with AzBio score in quiet. The result patterns remained the same regardless of whether the mean values of the IPGE slope and the PLV were weighted by the AzBio FIF. The IPGE slope and the PLV quantify different aspects of CN health. The positive association between the PLV and AzBio scores suggests that neural synchrony is important for speech perception in noise in adult CI users. The lack of association between age and the PLV indicates that reduced neural synchrony in the CN is unlikely the primary factor accounting for the greater deficits in understanding speech in noise observed in elderly, as compared to younger, CI users.

Sennoside A represses the malignant phenotype and tumor immune microenvironment of non-small cell lung cancer cells by inhibiting the TRAF6/NF-κB pathway.

Non-small cell lung cancer (NSCLC) is a prominent cause of cancer death worldwide. Sennoside A (SA) is the primary anthraquinone active component from Rheum officinale Baill and exerts antitumor functions in multiple human tumors. This research aimed to elucidate the function and mechanism of SA in NSCLC. SA functions in NSCLC were determined using Cell Counting Kit-8 (CCK-8) assay, Terminal deoxynucleotidyl transferase dUTP nick-end labeling analysis, Transwell assay, Enzyme-Linked Immunosorbent Assay (ELISA), and Western blot. Meanwhile, the SA mechanism in NSCLC was examined with Western blot, immunofluorescence assay, CCK-8 assay, Transwell analysis, and ELISA. Furthermore, SA functions in NSCLC growth in vivo were assessed by the establishment of a tumor xenograft model, hematoxylin-eosin staining, analysis of NSCLC tissue apoptosis, and immunohistochemistry assays. Functionally, less than 200µM SA had no significant effect on normal human bronchial epithelial cell BEAS-2B cell viability. Furthermore, H460 cell viability was decreased when the SA dose was greater than or equal to 25µM (IC50 = 53.34µM). A549 cell viability was reduced when the SA dose was greater than or equal to 12.5µM (IC50 = 48.21µM). Also, SA repressed NSCLC cell proliferation and boosted cell apoptosis. SA restrained NSCLC cell invasion and tumor microenvironment. Mechanically, SA weakened NSCLC cell proliferation, invasion, and tumor microenvironment, yet this impact was abolished after transfecting pcDNA3.1-TRAF6, which indicated that SA repressed NSCLC cell proliferation, invasion, and tumor microenvironment through inactivating TRAF6/NF-κB. Moreover, SA reduced subcutaneous tumor volume and promoted NSCLC tissue apoptosis in vivo. SA repressed NSCLC cell proliferation, invasion, and tumor microenvironment through inactivating TRAF6/NF-κB.

Identification and characterization of vasoactive intestinal peptide receptor antagonists with high-affinity and potent anti-leukemia activity.

Vasoactive intestinal peptide (VIP) is a neuropeptide involved in tumor growth and immune modulating functions. Previous research indicated that a VIP antagonist (VIPhyb) enhances T-cell activation and induces T-cell-dependent anti-leukemic activity in mice. We created a combinatorial library of VIPhyb C-terminal sequence variations to develop a more potent VIP-receptor (VIP-R) antagonist, hypothesizing that specific amino acid substitutions would improve receptor binding and plasma stability. In silico screening analyses identified sequences with improved docking scores predicting increased binding affinity to human VIP receptors VPAC1 and VPAC2. Fifteen peptides were synthesized and tested for their ability to potentiate activation of purified mouse and human T cells and enhance T cell-dependent anti-leukemia responses in murine models of acute myeloid leukemia. Treating C57Bl/6 mice engrafted with a C1498 myeloid leukemia cell line with daily subcutaneous injections of VIP-R antagonist peptides induced T cell activation resulting in specific anti-leukemia responses. Strikingly, the predicted binding affinity of the VIP-R antagonists to VIP receptors correlated positively with their ability to augment mouse T-cell proliferation and anti-leukemia activity. ANT308 and ANT195 emerged as top candidates due to their high predicted VIP-R binding, low EC 50 for in vitro T cell activation, and potent anti-leukemia activities. ANT308 decreased CREB phosphorylation, a downstream signaling pathway of the VIP receptor, and stimulated granzyme B and perforin expression in CD8+ T cells from AML patients. Combining in silico modeling, in vitro T cell activation properties, and in vivo anti-leukemia activity has identified promising VIP-R antagonist candidates for further development as novel immunotherapies for AML, especially for patients with relapsed disease.

Wnt 3a-Modified Scaffolds Improve Nerve Regeneration by Boosting Schwann Cell Function.

A pivotal approach in engineering artificial peripheral nerve sheaths encompasses the augmentation of the regenerative microenvironment via the manipulation of Schwann cells (SCs). Our investigation employed single-cell sequencing analysis to elucidate the potential functions of Schwann cells and the Wnt pathway in facilitating peripheral nerve regeneration. In vitro studies showed that activating the Wnt signaling pathway promotes the transition to repair SCs, boosting their growth, movement, and immune functions. To better understand the peripheral nerve regeneration environment, we created a polymer scaffold using ammonization and electrospinning. The Wnt3a protein was incorporated into the polycaprolactone (PCL) electrospun fiber surface. In a rat sciatic nerve defect model, the Wnt3a-modified scaffold showed better nerve repair outcomes than traditional electrospun scaffolds. After a week, the test group showed better immune regulation and angiogenesis, with a significant increase in axon growth rate observed after 3 weeks. Three-month-long animal experiments revealed notable improvements in neuroelectrophysiology, reduced organ atrophy, and enhanced sciatic nerve recovery. In this nerve defect model, Wnt3a-modified neural scaffolds achieved repair effects.

From Regulation to Application: The Role of Abscisic Acid in Seed and Fruit Development and Agronomic Production Strategies.

Abscisic acid (ABA) is a crucial plant hormone that plays a decisive role in regulating seed and fruit development and is becoming increasingly important in agricultural applications. This article delves into ABA's regulatory functions in plant growth, particularly during the stages of seed and fruit development. In the seed phase, elevated ABA levels help maintain seed dormancy, aiding seed survival under unfavorable conditions. During fruit development, ABA regulates pigment synthesis and sugar accumulation, influencing the nutritional value and market quality of the fruit. This article highlights three main strategies for applying ABA in agricultural production: the use of ABA analogs, the development of ABA signal modulators, and breeding techniques based on ABA signaling. ABA analogs can mimic the natural functions of ABA, while ABA signal modulators, including enhancers and inhibitors, are used to finely tune plant responses to ABA, optimizing crop performance under specific growth conditions. Furthermore, breeding strategies based on ABA signaling aim to select crop varieties that effectively utilize ABA pathways through genetic engineering and other technologies. ABA is not only a key regulator of plant growth and development but also holds great potential for modern agricultural practices.

The regulation of ovarian degeneration in Pampus argenteus by heat shock protein genes under low-temperature stress.

Temperature is a crucial environmental factor that significantly impacts the growth, development, metabolism, and physiological functions of fish. To study the effects of low-temperature on the gonadal development of silver pomfret (Pampus argenteus), a cold period by gradually lowering the water temperature from 18°C to 9°C was simulated. The results showed that hsp70, hsp90a, and hsp90b were widely expressed in the tissues of P. argenteus, with hsp70 primarily expressed in the pituitary, hsp90a and hsp90b mainly expressed in the lateral. The hsps were involved in the development process of P. argenteus from 1 to 27 days post-hatching (dph). The expression levels of hsp70 and hsp90b were highest at 17 dph, while the levels of hsp90a were at 25 dph. Under the condition of 9°C, regressed oocyte were observed in the ovaries, the oocyte diameter significantly decreased, and the ovaries degenerated 100% after low-temperature stress. The expression levels of hsps in the ovaries were significantly higher than in the control group, while the expression levels in the testes were significantly lower than in the control group. Taken together, hsps may regulate the ovarian degeneration under low-temperature stress. Male fish rapidly completed meiosis and maintained the testes in the prophase of meiosis to resist the low-temperature stress.