This paper firstly investigated fire dynamics characteristics and the temperature field distributions of large space spherical mesh shell (SMS) structures under localized fire in FDS, and divided the temperature fields into plume region, smoke accumulation region and ceiling jet region. Based on the classical axisymmetric plume model, the predictive models for the steady-state and transient temperature fields in different fire regions for large space SMS structures were proposed in this paper. Thereafter, the proposed temperature models were compared with the simulated results and experimental test results by other scholars, and they were in good agreement. In order to further discover the realistic fire development progress and temperature distribution of large space fire, and validate the proposed temperature field models, this paper designed and conducted three full-scale large space fire tests (Test-1 ∼ Test-3) with different fire source powers in a large space building. Results showed that temperature fields of large space fires could be consisted of near-fire region (Flame region) and far-fire region (Plume region and Ceiling jet region). The steady-state fire source powers were tested for 443 kW, 886 kW, 1090 kW for Test-1, Test-2 and Test-3, respectively, while their maximum temperatures of the roof reached 75 °C, 105 °C and 120 °C. Based on McCaffrey plume model, we further proposed the transient temperature model in flame region for large space structures, and its accuracy was verified by the test results. Moreover, the proposed steady-state and transient temperature fields for large space SMS structures were compared with the tested temperature field data, and the tested and predicted temperature curves were generally in good agreement in plume and ceiling jet regions. Finally, the test results aimed to provide a scientific basis and reference for the fire safety and structural fire-resistant design of large space buildings.