Full-field Optical Coherence Tomography Setup Research Articles

Analysis of the impact of optical aberrations in en-face full-field OCT microscopy.

Optical coherence tomography (OCT) is a powerful technique for cross-sectioning imaging. However, the lateral resolution may be degraded by optical aberrations originating from the sample or the setup. We present an extensive quantitative study of the impact of aberrations in time-domain en-face full-field OCT (FFOCT). Using an adaptive optics loop integrated in an FFOCT setup, a deformable mirror is used to introduce low-order calibrated aberrations. The experimental analysis of both the line spread functions (SF) and the complex object images has allowed us to measure the loss in contrast and the impact on lateral spatial resolution. We demonstrate that the frequency content of FFOCT image spectra in terms of signal-to-noise ratio and cutoff frequency is degraded by aberrations but remains much higher than in conventional incoherent images. Line SF profiles in conventional imaging display widening, whereas in FFOCT they display oscillations, leading to the possible perception of preserved resolution. Nevertheless, for complex objects, the aberration image blurring is strong due to the convolution process by the point SF, resulting in a significant filtering of the image spatial spectrum.

Full-field optical coherence tomography—An educational setup for an undergraduate lab

Optical coherence tomography, or in short OCT, is a measurement technique established in the early 1990s for the non-invasive imaging of interfaces in the bulk of biological tissues or other samples. A full-field OCT setup is built from a microscope combined with a Michelson interferometer, where the mirror in one arm is replaced by the sample. Using white light, which is temporally partially coherent, interference fringes disclose the presence of an interface whenever the lengths of both interferometer arms are nearly equal. Scanning one arm allows for a volumetric reconstruction of all interfaces inside the sample. While the importance of OCT in medicine is indisputable, it is hard to teach students the basic aspects of such technology as most available setups tend to be rather complex. It is our purpose to present a fully functional full-field OCT setup that is stripped-down to its essential components and to promote its use in an undergraduate lab course. The contribution is complemented by a description of the basic theory necessary to understand the working principle of OCT.

Full-Field Swept Source Master-Slave Optical Coherence Tomography

We apply the principle of master-slave (MS) interferometry to a full-field swept source optical coherence tomography (OCT) setup equipped with a fast 2-D camera. MS interferometry does not involve Fourier transformations and, therefore, eliminates the need for spectrum data resampling required by the conventional spectral domain OCT. Using this method in a full-field OCT setup, en face images are acquired in vivo from finger skin, Drosophila melanogaster larvae, and pupae, with no spectrum resampling and no mechanical scanning. The signal processing is based on a comparison operation of the shapes of channeled spectra for each camera pixel, with a set of reference signals (masks). In this way, en face OCT images can be obtained with no need for the volumetric assembly and software cutting the en face images from an image volume, which are required by the conventional spectral domain OCT method. We demonstrate that the sensitivity and axial resolution of the MS method in a full-field swept source OCT setup are similar to the values obtained using the conventional Fourier-transformation-based swept source OCT method in a full-field setup. Multiple en face images can be produced in parallel by using multiple stored shapes of channeled spectra for the depths of interest. The full-field MS-OCT method presented here opens the possibility of parallel processing for all image points in a 3-D volume of the object.

Rapid evaluation of fresh ex vivo kidney tissue with full-field optical coherence tomography

Background: Full-field optical coherence tomography (FFOCT) is a real-time imaging technique that rapidly generates images reminiscent of histology without any tissue processing, warranting its exploration for evaluation of ex vivo kidney tissue. Methods: Fresh tissue sections from tumor and adjacent nonneoplastic kidney (n = 25 nephrectomy specimens; clear cell renal cell carcinoma (CCRCC) = 12, papillary RCC (PRCC) = 4, chromophobe RCC (ChRCC) = 4, papillary urothelial carcinoma (PUC) = 1, angiomyolipoma (AML) = 2 and cystic nephroma = 2) were imaged with a commercial FFOCT device. Sections were submitted for routine histopathological diagnosis. Results: Glomeruli, tubules, interstitium, and blood vessels were identified in nonneoplastic tissue. In tumor sections, the normal architecture was completely replaced by either sheets of cells/trabeculae or papillary structures. The former pattern was seen predominantly in CCRCC/ChRCC and the latter in PRCC/PUC (as confirmed on H&E). Although the cellular details were not very prominent at this resolution, we could identify unique cytoplasmic signatures in some kidney tumors. For example, the hyper-intense punctate signal in the cytoplasm of CRCC represents glycogen/lipid, large cells with abundant hyper-intense cytoplasm representing histiocytes in PRCC, and signal-void large polygonal cell representing adipocytes in AML. According to a blinded analysis was performed by an uropathologist, all nonneoplastic tissues were differentiated from neoplastic tissues. Further, all benign tumors were called benign and malignant were called malignant. A diagnostic accuracy of 80% was obtained in subtyping the tumors. Conclusion: The ability of FFOCT to reliably differentiate nonneoplastic from neoplastic tissue and identify some tumor types makes it a valuable tool for rapid evaluation of ex vivo kidney tissue e.g. for intraoperative margin assessment and kidney biopsy adequacy. Recently, higher resolution images were achieved using an experimental FFOCT setup. This setup has the potential to further increase the diagnostic accuracy of FFOCT.

In vivo and in situ cellular imaging full-field optical coherence tomography with a rigid endoscopic probe

Full-field OCT has proved to be a powerful high-resolution cellular imaging tool for biological tissues. However the standard bulk full-field OCT setup does not match the size requirements for most in situ and in vivo imaging applications. We adapted its principle into a rigid needle-like probe using two coupled interferometers and incoherent illumination: an external processing interferometer is used for in-depth scanning, while a distal common-path interferometer at the tip of the probe collects light backscattered from the tissue. Our experimental setup achieves an axial and transversal resolution in tissue of 1.8 µm and 3.5 µm respectively, for a sensitivity of −80 dB. We present ex vivo images of human breast tissue, and in vivo images of different areas of human skin, which reveal cellular-level structures.

Simultaneous dual-band ultra-high resolution full-field optical coherence tomography

Ultrahigh-resolution full-field optical coherence tomography (FF-OCT) is demonstrated in the 800 nm and 1200 nm wavelength regions simultaneously using a Silicon-based (Si) CCD camera and an Indium Gallium Arsenide (InGaAs) camera as area detectors and a halogen lamp as illumination source. The FF-OCT setup is optimized to support the two broad spectral bands in parallel, achieving a detection sensitivity of approximately 90 dB and a micrometer-scale resolution in the three directions. Images of ex vivo biological tissues are presented (rabbit trachea and Xenopus laevis tadpole) with an increase in penetration depth at 1200 nm. A color image representation is applied to fuse both images and enhance spectroscopic property visualization.

Optical 3D-storage in sol–gel materials with a reading by optical coherence tomography-technique

We report on the recording of 3D optical memories in sol–gel materials by using a non-linear absorption effect. This effect induces a local change in optical properties of the material which is read and quantified with a high-resolution full-field optical coherence tomography setup. It is the first time that this technique is used for this purpose. Data recording was performed by focused picosecond (ps) single-pulse irradiation at 1064 nm with energy densities of 10 and 33 J/cm 2 per pulse.