Full-fat Flaxseed Research Articles

PSVI-19 Abundance of some cecal bacteria in Lohmann LSL lite pullets and hens fed omega-3 fatty acids and yeast bioactives in low or high spacing allowance

Abstract Nutritional optimizations and rearing environment that meets welfare standards are prerequisite in the performance and health of laying hens. Rearing practices influence gastrointestinal development, microbiota, and immune function. However, little is known on the implications of rearing spacing allowance (SA) and feed ingredients on intestinal microbiota. We determined the abundance of selected cecal bacteria in pullets and hens in response to rearing SA and dietary omega-3 fatty acids (N-3 FA) and yeast bioactive (YB) from placement to 17 wk of age (woa). One-day old chicks (n = 2,632) were placed in cages in 2 SA of 24 cages each: High SA (HSA, 348 cm2/bird; ~50 birds/cage) and Low SA (LSA, 284 cm2/bird; ~68 birds/cage). Birds were fed corn and soybean-based diets (C), C + 3% co-extruded full fat flaxseed and pulse mixture (N-3 FA) and C + 0.05% YB processed with β-1,3-glucan hydrolase (YB). Birds were allocated to diets within SA according to 2 x 3 factorial design (n = 8 cage/treatment) and fed ad libitum. At 4 and 16 woa, 1 and 2 pullets/treatment respectively, were necropsied for ceca digesta from each cage and independently analyzed. At 18 woa, hens were placed in enriched cages based on rearing treatment identities in egg production facility and fed a commercial diet. At 35 woa, 18 hens per treatment were necropsied for ceca digesta samples and analyzed independently. Samples were analyzed for viable bacterial counts for E. coli, Enterococcus and Lactobacillus. Statistical analysis was performed by general linear mixed model procedures of SAS with diets, SA, sampling age and associated interactions as fixed factors, difference with P < 0.05 was considered significant. Comparison of log-transformed means (CFU/g) showed that diets and SA had no significant effect on cecal E. coli counts at 4, 8 and 35 woa (P > 0.05; Table 1). However, at 16 woa the least E. coli counts was found in LSA (P < 0.0001), with N-3-FA-fed and YB-fed pullets having fewer numbers (P = 0.007) than control-fed pullets. There was no interaction effects between diet and density for E. coli in all sampling wk (P >0.05). No significant effect of SA or diets were observed at any sampling week for Enterococcus counts, except LSA had a tendency at 8 woa (P = 0.078). At 8 woa, the cecal Lactobacillus counts (P = 0.009) were less in LSA relative to HSA pullets. In conclusion, feeding of N3-FA or YB during rearing influenced cecal microbiota. Supplementation of YB seemed to promote growth of beneficial Lactobacillus, which is associated with strengthened immunity.

489 Antibiotic Resistance of cecal Escherichia coli isolates from Lohmann LSL lite pullets and laying hens fed omega-3 fatty acids or yeast bioactives, reared at different spacing allowance

Abstract Poultry diets provide components that alter gut microbiota composition. We investigated antimicrobial resistance (AMR) phenotypes of E. coli isolates from ceca digesta of pullets and hens reared at different spacing allowance (SA) and dietary source of omega-3 fatty acids (N-3 FA) and yeast bioactives (YB) from placement to 17 wk of age (woa). A total of 2,632-day old chicks were placed in cages at two SA (24 cages each): High SA (HSA, 348 cm2/bird; ~50 birds/cage) and Low SA (LSA, 284 cm2/bird; ~68 birds/cage). Birds were fed corn and soybean-based diets (C), C + 3% co-extruded full fat flaxseed and pulse mixture (N-3 FA) and C + 0.05% YB processed with β-1,3-glucan hydrolase. At 4, 16 and 35 woa, ceca samples were collected to isolate E. coli using Chromocult agar. Their susceptibility to 14 antibiotics were determined by automated Sensititre system according to CLSI and CIPARS guidelines to determine their antimicrobial susceptibility profile. Number of antibiotics resistant E. coli isolates were analyzed using general linear mixed model procedure of SAS with diets, SA, sampling age and associated interactions as fixed factors. Cochran-Mantel-Haenszel test was used to determine the relationship between these treatments, and differences between treatments were considered significant at P < 0.05. Out of 428 presumptive E. coli isolated, 153 of 428 isolates (35.7%) were resistant to one antimicrobial class but no resistance against ciprofloxacin, azithromycin and nalidixic acid were detected. The prevalence of antimicrobial resistant E. coli decreased as the birds aged from wk 4–35 (P < 0.05). At wk 4 (P = 0.002) and 16 (P = 0.011), the least prevalence of ampicillin (AMP) resistance was in ceca digesta from N-3 FA-fed birds. At 35 woa, no AMP and cefoxitin (FOX) resistance were detected in birds fed YB (Table 2). At 16 woa, the prevalence of streptomycin resistance was greater in N-3 FA-fed than in YB-fed birds (P = 0.02), which was less than control-fed birds. Of the 428 isolates, 59 (13.8%) were resistant to more than two antimicrobial classes, with most from TIO-FOX-AMP-TET and STR-SULF-TET. At wk-4, SA had a significant effect on multiple resistance spectrum with LSA having the lowest MDR E. coli (P = 0.02) mainly from N-3 FA-fed pullets. This study showed an overall low AMR prevalence which decreases with age of the bird. This study is the first to report that feed additives (N-3 FA or YB) influence the AMR profile of cecal E. coli in pullets and layers. Prevalence of AMP resistance was least in N-3 FA-fed birds, whereas least STEP- and SULF resistance was observed in YB-fed birds. Our next study would help understand the molecular and evolutionary aspect of this resistant E. coli and their potential to cause colibacillosis.

Significance of successive feeding of sources of n-3 fatty acids to broiler breeders and their progeny on growth performance, intestinal lesion scores, lymphoid organs weight and plasma immunoglobulin A in broiler chickens challenged with Eimeria

The study examined the effects of successive feeding of sources of n-3 PUFA to broiler breeders (BB) and their progeny in broiler chickens challenged with Eimeria. The BB were fed: 1) control (CON), corn-soybean meal diet, 2) CON + 1 % microalgae (DMA), as a source of DHA and 3) CON + 2.50% co-extruded full fat flaxseed (FFF), as a source of ALA. Eggs were hatched at 34, 44, and 54 wk of age. Posthatch treatments (BB-progeny) were: CON-CON, DMA-CON, FFF-CON, DMA-DMA and FFF-FFF with diets formulated for starter (d 1-10) and grower/finisher (d 11-42) phases. All chicks were orally challenged with Eimeria (E. acervulina and E. maxima) on d 10. Relative to CON, DMA and FFF increased concentration of n-3 PUFA by ≥ 2-fold in hatching eggs and progeny diets. There were no (P > 0.05) interactions between treatment and BB age on d 0 to 10 growth. In general, BB age affected (P < 0.05) growth performance throughout the study. In the starter phase, successive exposure to DHA and ALA improved FCR over CON-CON (P < 0.01). The interaction between treatment and BB age in grower/finisher was such that DHA exposure to younger BB resulted in poor growth performance (P < 0.05) relative to exposure to older BB. In contrast, exposure to ALA had similar (P > 0.05) growth performance irrespective of BB age. Moreover, successive exposure to ALA resulted in higher BWG, breast weight and lower FCR compared to successive exposure to DHA (P < 0.05). There were no (P > 0.05) interactions between treatment and BB age on the intestinal lesion scores, lymphoid organ weights and concentration of plasma immunoglobulin A (IgA). Successive exposure to DHA resulted in higher (P = 0.006) jejunal lesion scores than CON-CON birds. The results showed that successive exposure of DHA and ALA improved FCR relative to non-exposed birds in the starter phase. However, responses in the grower/finisher phase depended on n-3 PUFA type, with birds on successive ALA exposure supporting better growth and breast yield than birds on successive DHA exposure.

Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of age

Growth and response to Escherichia coli lipopolysaccharide (LPS) challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids (n-3 FA) and yeast bioactives (YB) from hatch through to 16 wk of age (woa) were investigated. Co-extruded full fat flaxseed and pulse mixture (FFF; 1:1 wt/wt) supplied n-3 FA and YB were yeast cell walls processed with β-1,3-glucan hydrolase. A total of 1,064-day-old pullets were placed in cages (19 birds/cage) and allocated to 7 diets (n=8). The iso-caloric and iso-nitrogenous diets were control, control+1, 3, or 5% FFF and+0.025, 0.05, or 0.1% YB. The birds had ad libitum access to feed and water. Body weight (BW), feed intake, and lymphoid organs weight were recorded. At 15 woa, 2 pairs of pullets/cage received intravenous injection of either 1 mL of sterile saline without or with 8 mg LPS/kg BW. Injected pullets were bled, monitored for BW and cloaca temperature at time points within 168 h. Birds fed 1% FFF were heavier (P < 0.04) than birds fed other diets at 16 woa with FFF and YB exhibiting non-linear responses. Control birds had heavier (P=0.02) thymus at 4 woa. Spleen weight increased quadratically (P < 0.05) in response to FFF at 8 and YB at 16 woa. The LPS increased cloaca temperature and altered concentration of several plasma metabolites (P < 0.05). Theinteraction (P < 0.05) between LPS and diet was such thatcontrol birds exhibited lower creatine kinase (CK) upon challenge with LPS relative to birds fed other diets. The LPS birds fed 1% FFF and 0.05% YB showed higher plasmaalbumin than non-LPS cohorts. Non-LPS birds fedcontrol, 1 and 3% FFF had higher plasma K than LPS cohorts. In general, FFF and YB exhibited linear and quadratic effects (P < 0.05) onselect plasma metabolites. In conclusion, dietary provision of n-3 FA and YB influenced pullet BW at sexual maturity, development of lymphoid organs and modulated some plasma metabolites in response to LPS.

Influence of designer diet and holy basil (Ocimum sanctum) leaves on yolk colour, yolk carotenoid, and immunity status of layers

A biological experiment of six weeks duration, followed by several laboratory investigations was carried out to study the effect of designer layer mash (DLM) containing full fat flaxseed, oil rich sardine fish, holy basil leaf meal (BLM), vitamin E and organic selenium (Sel-plex), on yolk colour, carotenoid and immune status of layers. Both designer diet and BLM had significantly improved the yolk colour and pigment levels. They also exhibited a synergistic effect. The DLM had significantly increased the vitamin E and selenium levels in the egg; but BLM had no such effect. The immune status of the layers, expressed as Immunoglobulin IgY and HI titre, indicated that both designer diet as well as the BLM had significantly improved the IgY levels and HI titre.

Body weight, organ development and jejunal histomorphology in broiler breeder pullets fed n-3 fatty acids enriched diets from hatch through to 22 weeks of age

Dietary long chain polyunsaturated n-3 fatty acids (n-3 FA) may be beneficial to broiler breeder (BB) development. Therefore, the effects of feeding sources of docosahexaenoic acid (DHA) and α-linolenic acid (ALA) from hatch through to 22 weeks of age (woa) on growth, organ weight, and jejunal histomorphology were investigated. A total of 588-day-old Ross × Ross 708 BB were reared on one of 3 diets: 1) control, corn-soybean meal diet, 2) Control + 1% microalgae (DMA, Aurantichytrium limacinum), as a source of DHA and 3) Control + 2.50% co-extruded full fat flaxseed and pulse mixture (FFF, 1:1 wt/wt), as a source of ALA. Diets DMA and FFF had similar total n-3 and n-6: n-3 ratio. Diets were allocated to floor pens (28 birds/pen) to give 9 or 6 replicates per diet for control or DMA and FFF, respectively and fed according to breeder curve in 3 phases: starter (0–4 woa), grower (5–19 woa), and pre-breeder (20–22 woa). Individual body weight (BW) was taken weekly and 6 birds/pen necropsied at 5 and 12 woa for gastrointestinal, spleen, bursa, and liver weight and samples for jejunal histomorphology. There was no (P > 0.05) diet effect on growth by 20 woa. With exception of 5 woa, pullets fed DMA showed (P < 0.001) lower BW coefficient of variation (C.V.) than pullets fed control between 2 and 7 woa. However, pullets fed DMA had higher BW CV at 20 woa than birds fed either control or FFF. At 5 woa, birds fed DMA had taller (P ≤ 0.01) villi and deeper crypt than birds fed either control or FFF but VH or CD were similar (P > 0.05) between CON and FFF pullets. At 12 woa, birds fed FFF had taller VH than birds fed control diet but similar (P > 0.05) to that of birds fed DMA. Therefore, different responses to sources of omega-3 FA may implicate other components, however, the BW uniformity and intestinal histomorphology responses suggested benefits of feeding omega-3 FA during rearing.

Effect of Escherichia coli lipopolysaccharide challenge on eggshell, tibia, and keel bone attributes in ISA brown hens exposed to dietary n-3 fatty acids prior to onset of lay

The impact of Escherichia coli lipopolysaccharide (LPS) challenge on eggshell, tibia, and keel bone characteristics in ISA brown hens derived from breeders and pullets fed omega-3 polyunsaturated fatty acids (n-3 PUFA) was examined. The breeders were fed the following diets: 1) Control (CON); 2) CON + 1% microalgae as the source of docosahexaenoic acid (DHA); and 3) CON + 2.6% of a co-extruded mixture of full-fat flaxseed and pulses as a source of α-linolenic acid (ALA). During the pullet phase, offspring from breeders fed CON were fed CON or supplemented diets, and offspring from supplemented diets either continued with respective n-3 PUFA diets or CON. At 18 weeks of age (WOA), pullets were fed a common layer diet to 42 WOA. A total of 5 birds were selected based on the average body weight (BW) of each treatment and moved to an individual cage at 41 WOA. Three days before the end of 42 WOA, all the birds were weighed and subcutaneously injected with either saline or 4 mg LPS/kg BW. Eggs were recorded, labeled, and kept for egg quality analyses. At 42 WOA, birds were necropsied for tibia and keel bone samples. Administration of LPS reduced eggshell breaking strength, eggshell weight, tibia, and keel bone ash content (P < 0.05). Specifically, LPS challenged hens had 14.9, 11.1, 9.2, and 11.6% lower eggshell breaking strength, eggshell weight, keel, and tibia ash content, respectively relative to unchallenged hens. Hens from breeders and pullets fed n-3 PUFA had similar (P > 0.05) eggshell, tibia, and keel bone attributes to control hens. In conclusion, the provision of ALA and DHA to breeders and their offspring did not alleviate the negative effects of LPS on eggshell, tibia, and keel bone characteristics in laying hens.

Impact of feeding n-3 fatty acids to layer breeders and their offspring on concentration of antibody titres against infectious bronchitis, and Newcastle diseases and plasma fatty acids in the offspring

ABSTRACT 1. The impact of feeding sources of n-3 fatty acids (FA) to ISA Brown and Shaver White breeders and their offspring on antibody titres and plasma FA profile was examined. 2. Breeders were fed either a control diet (CON); CON + 1% microalgae (DMA: Aurantiochytrium limacinum) as a source of docosahexaenoic acid; or CON + 2.6% of a co-extruded mixture of full-fat flaxseed (FFF) as a source of α-linolenic acid. Day-old female offspring were assigned to diets (breeder-offspring): 1) CON-CON, 2) CON-DMA, 3) CON – FFF, 4) DMA – CON, 5) DMA – DMA, 6) FFF – CON or 7) FFF – FFF, followed by a standard layer diet through 18 weeks of age (WOA) to 42 WOA. 3. Antibody titres against infectious bronchitis (IBV) and Newcastle disease (NDV) were measured at six days and six WOA, and plasma FA profile was measured at 18 and 42 WOA. 4. Pullets from FFF-fed breeders had higher antibody titres against IBV and NDV than pullets fed DMA (P < 0.05). Feeding FFF to offspring increased plasma ∑n-3 FA at 18 and 42 WOA, whereas feeding DMA to offspring reduced ∑n-6 FA at 18 WOA. 5. In conclusion, independent of breeder strain, alpha linoleic acid (ALA) and DHA sources showed varied responses. Feeding breeders FFF increased plasma concentration of antibody titres and n-3 FA whereas DMA reduced plasma concentration of ∑n-6 FA.

Effects of feeding ISA brown and Shaver white layer breeders with sources of n-3 fatty acids on hatching egg profiles, apparent embryonic uptake of egg components, and body composition of day-old chicks

Effects of feeding ISA brown and Shaver white breeders sources of n-3 fatty acids (FA) on egg components, apparent embryonic uptake (AEU) of egg components, and hatching body composition were examined. A total of 240 females and 30 males per each strain were fed either: (1) control (CON); (2) CON + 1% of dried microalgae (DMA), as a source of docosahexaenoic acid; or (3) CON + 2.60% of dry extruded product consisting of full-fat flaxseed (FFF), as a source of α-linolenic acid for 30 d. Eggs were incubated and the residual yolks (RY) sampled at hatch for AEU of dry matter (DM), minerals, and organic matter (OM). Feeding n-3 FA sources reduced the AEU of OM and minerals resulting in a higher ratio of RY to body weight (P = 0.002). Feeding FFF increased body fat and decreased lean in Shaver white hatchlings compared with CON (P &lt; 0.05). The body mineral was reduced by feeding DMA compared with other treatments (P &lt; 0.05). The change in body composition in response to feeding of n-3 sources was associated with the change in AEU of DM, OM, and minerals, not the concentration of these components in the yolk.

Standardized ileal digestible amino acid contents in flaxseed and flaxseed meals fed to growing–finishing pigs

A total of 10 cannulated barrows with an average body weight of 55.99 ± 3.73 kg were assigned to test diets containing either full-fat flaxseed (FFS), expeller-pressed flaxseed meal (expeller-FM), or solvent-extracted flaxseed meal (solvent-FM). The standardized ileal digestibility (SID) of methionine and threonine was higher (P &lt; 0.05) in FFS than in expeller-FM. Similarly, the SID of crude protein (CP) and threonine was higher (P &lt; 0.05) in FFS than that in solvent-FM. However, expeller-FM and solvent-FM had greater (P &lt; 0.05) contents of SID CP, arginine, leucine, phenylalanine, aspartic acid, glutamic acid, and serine than FFS.

N-3 fatty acids fed to ISA brown and Shaver white breeders and their female progeny during rearing: Impact on egg production, eggshell, and select bone attributes from 18 to 42 weeks of age

The impact of feeding sources of n-3 fatty acids (FA) to ISA brown and Shaver white breeders and their female offspring during rearing on egg production, eggshell, tibia, and keel bone attributes was examined. Breeders were fed Control (CON) or CON + 1% dried microalgae (DMA: Aurantiochytrium limacinum) as the source of docosahexaenoic acid or CON + 2.6% of a coextruded mixture of full-fat flaxseed (FFF) and pulses as a source of α-linolenic acid. Day-old offspring were fed 1) breeder CON-pullet CON (CON-CON), 2) breeder CON-pullet DMA (CON-DMA), 3) breeder CON-pullet FFF (CON-FFF), 4) breeder DMA-pullet CON (DMA-CON), 5) breeder DMA-pullet DMA (DMA-DMA), 6) breeder FFF-pullet CON (FFF-CON), and 7) breeder FFF-pullet FFF (FFF-FFF). At 18 wk of age (WOA), pullets were fed a common layer diet to 42 WOA for egg production and bone quality assessments. There was no (P > 0.05) interaction between strains and diets and the main effect of diets on egg production, egg mass, and eggshell quality. There was an interaction (P = 0.008) between strain and diet on egg weight (EW); however, the strain effect on EW (P < 0.001) was such that ISA brown had heavier eggs than Shaver white. Shaver white had higher (P < 0.001) eggshell %, eggshell, and tibia breaking strength (BS), as well as tibia ash concentration compared with ISA brown hens. In contrast, ISA brown hens exhibited heavier (P < 0.05) tibia and keel bones. Feeding breeders DMA and pullets both sources of n-3 FA increased tibia medullary ash concentration compared with other diets (P < 0.001). Shaver white hens showed greater decline in tibia BS (83.7 vs. 96.3%) and ash content (84.1 vs. 94.3%) than ISA brown hens from 18 to 42 WOA (P < 0.05). Strain and diets exhibited independent effects on eggshell, tibia, and keel attributes. Provision of α-linolenic acid and docosahexaenoic acid to breeders and offspring improved tibia medullary ash concentration at 42 WOA.

Effects of feeding broiler breeder hens a coextruded full-fat flaxseed and pulses mixture without or with multienzyme supplement

The effects of coextruded full-fat flaxseed and pulses (FFF; 1:1 wt/wt) mixture on n-3 polyunsaturated fatty acids (PUFA) enrichment in egg yolk, hepatic attributes, apparent retention (AR) of components, and ceca metabolites were evaluated in broiler breeder hens. The diets were as follows: 1) corn–soybean control, 2) control diet plus 18% FFF (FFF−), and 3) FFF plus enzyme supplement (FFF+) containing galactanase, protease, mannanase, glucanase, xylanase, amylase, and cellulase activities. Twenty-six-week-old Cobb 500 broiler breeder hens were allocated to 30 identical cages (2 hens/cage) and given 1-week adaptation period. The 3 diets were assigned to 10 replicate cages based on postadaptation BW and fed based on breeder curve for 30 D. Excreta samples were collected from day 24 to 27 for determination of AR of components, and eggs were collected from day 28 to 30 for yolk polyunsaturated fatty acids analyses. On day 30, birds were weighed, killed via cervical dislocation, liver weighed, and stored for fat analyses. Ceca digesta samples were taken for concentration of short-chain fatty acids. Liver and yolk weights as well as total yolk FA were not influenced by diets (P > 0.05). Control birds had lower yolk concentration of α-linolenic acid than birds fed either FFF− or FFF+ (P < 0.01) corresponding to 7.5, 36.8, and 37.3 mg/g for the control, FFF−, and FFF+, respectively. Control birds also exhibited lower yolk concentration of docosahexaenoic acid (P < 0.01). Control birds had higher hepatic concentration of crude fat and apparent retention of dry matter and crude protein compared with either the FFF− or FFF+ birds (P < 0.05). Birds fed FFF- diet had lower ceca digesta concentration of lactic acid than control and FFF+ (P < 0.05) birds. Results showed broiler breeder hens enriched egg yolk with n-3 polyunsaturated fatty acids without effects on the liver while the supplemental enzyme did not improve the utilization of FFF.

Impact of feeding microalgae (Aurantiochytrium limacinum) and co-extruded mixture of full-fat flaxseed as sources of n-3 fatty acids to ISA brown and Shaver white breeders and progeny on pullet skeletal attributes at hatch through to 18 weeks of age

Impact of feeding n-3 fatty acids (FA) to ISA brown and Shaver white breeders and their progeny on bone development in pullets was investigated. Breeders were fed Control (CON); CON + 1% microalgae (DMA: Aurantiochytrium limacinum) as the source of docosahexaenoic acid; and CON + 2.6% of a co-extruded mixture of full-fat flaxseed (FFF) and pulses mixture as source of α-linolenic acid. Test diets (DMA and FFF) were balanced for total n-3 FA and n-6: n-3 FA ratio. Samples of day-old progeny were euthanized for bone mineral content (BMC) and tibia collagen type II. The remaining pullets were fed posthatch treatments as follows: from breeder CON: CON (CON-CON), DMA (CON-DMA), and FFF (CON-FFF), from breeder DMA: CON (DMA-CON) and DMA (DMA-DMA) and from breeder FFF: CON (FFF-CON) and FFF (FFF-FFF). A total of 60 pullets per posthatch diets were reared in cages (12 pullets/cage, n = 5) with free access to feed and water, bled at 6, 12, and 18 wk of age (WOA) for bone turnover markers and necropsied at 18 WOA for tibia and femur samples. Day-old pullets from breeder fed CON had greater BMC (P < 0.001) relative to those from breeders fed other diets. There was strain and diet interaction (P ≤ 0.024) on tibia breaking strength (TBS) and tibia cortical ash concentration at 18 WOA such that diet responses were only observed in Shaver white pullets. In this context, TBS of DMA-DMA and FFF-FFF was greater than for pullets originating from CON breeder, and the cortical ash weight of DMA-DMA and FFF-FFF pullets was 23.8 and 20.2%, respectively, higher than for CON-CON pullets. In conclusions, the strain effects were strong on tibia attributes on 18-week-old pullets. Breeder feeding of n-3 FA was more effective when concomitant with posthatch feeding of n-3 FA in supporting the skeletal strength and cortical bone development in Shaver white pullets. Further investigations are warranted to establish the impact these strategies on skeletal health during laying cycle.

Effect of full-fat sunflower or flaxseed seeds dietary inclusion on performance, egg yolk fatty acid profile and egg quality in laying hens.

A study was conducted to evaluate the effect of the dietary inclusion of full-fat flaxseed or full-fat sunflower seeds on performance parameters, egg quality parameters and egg yolk fatty acid profile in laying hens. A total of 150 Babcock Brown hens at 27 weeks of age were distributed in 3 experimental treatments, as follows: T1, control; T2, diet containing 13.5% full-fat whole flaxseed seeds; and T3, diet containing 13.5% full-fat ground sunflower seeds. Feed and water were provided ad libitum and the experiment lasted for 8 weeks. No significant differences were found on egg quality parameters, but total egg production and rate of lay were significantly (P < 0.05) lower in the group receiving sunflower seeds compared with the control, and significant differences (P < 0.05) in egg yolk saturated, monounsaturated, polyunsaturated, omega-6 (n-6) and omega-3 (n-3) fatty acids were observed, as well as in the n-6:n-3 ratio. The results show that the dietary inclusion of 13.5% flaxseed full-fat seeds significantly increases the n-3 polyunsaturated fatty acid (PUFA) content in the eggs and lowers the n-6:n-3 ratio without affecting performance parameters. Inclusion of full-fat sunflower seeds increases the n-6 PUFA content but affects total egg production and rate of lay. Further studies are needed to determine the level of inclusion of full-fat sunflower seeds that does not affect performance.

Enriching ISA brown and Shaver white breeder diets with sources of n−3 polyunsaturated fatty acids increased embryonic utilization of docosahexaenoic acid

There is limited information on feeding egg-type chick breeders n−3 polyunsaturated fatty acids (PUFA) and its impact on hatching egg quality and embryonic fatty acid (FA) utilization. We investigated the effects of feeding brown and white egg-type chick breeders diets containing sources of n−3 PUFA on egg composition, apparent embryonic FA utilization, and intestinal FA transporter in hatchlings. Twenty-six-week-old ISA brown and Shaver white breeders were fed either 1) control (CON); 2) CON + 1% of microalgae (DMA, Aurantiochytrium limacinum) fermentation product, as a source of docosahexaenoic acid (DHA); or 3) CON + 2.60% of coextruded full-fat flaxseed and pulse mixture (FFF, 1:1 wt/wt) as a source of α-linolenic acid (ALA). Test diets had similar total n−3 and n−6:n−3 ratio. Eggs were hatched, and residual yolk (RY) samples taken for FA analyses. Apparent embryonic FA utilization was calculated by subtracting concentration of FA in RY from concentration of FA in yolk before incubation. There was an interaction between strains and diets (P < 0.05) on DHA in phospholipid and triglyceride fractions of yolk. Both n−3 PUFA sources increased DHA to a greater extent in Shaver white than in ISA brown. The interactive effect of strains and diets (P = 0.019) on embryonic utilization of ALA was such that DMA and FFF reduced ALA utilization, and this pattern was more prevalent in Shaver white birds than in ISA brown birds. There was no interaction between strains and diets on DHA utilization (P > 0.05). Embryos from hens fed n−3 PUFA sources used less total FA in phospholipid fraction (P < 0.001), and they preferentially used more DHA than CON embryos. Shaver white embryos used more (P < 0.05) ALA and DHA than ISA brown embryos. Although data suggested Shaver white had higher propensity of depositing DHA than ISA brown, irrespective of strain, feeding n−3 PUFA modified embryonic pattern of FA utilization toward utilization of DHA.

Molecular changes of phenolic-protein interactions in isolated proteins from flaxseed and soybean using Native-PAGE, SDS-PAGE, RP-HPLC, and ESI-MS analysis.

The effects of protein-phenolic interactions on the molecular characteristics of soybean and flaxseed proteins were investigated. Proteins were isolated from soybean and flaxseed using isoelectric precipitation, followed by extraction of free and bound phenolics. The effects of elimination of the phenolic compounds on molecular characteristics of the protein isolates were studied using reversed phase-high performance liquid chromatography (RP-HPLC), Native and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and electron spray ionization-mass spectrometry (ESI-MS). The Native-PAGE fractions from isolated proteins from full-fat flaxseed and soybean revealed that protein migration was affected by removal of bound phenolics. SDS-PAGE from full-fat and defatted protein isolates of flaxseed and soybean revealed that the removal of bound phenolics affected the molecular characteristics of protein subunits. Soybean protein isolates had protein-phenolic interactions through acidic and basic subunits. RP-HPLC and ESI-MS showed that the removal of bound and free phenolics had only minor effects on the molecular characteristics of isolated proteins from defatted and full-fat soybean. With respect to isolated proteins from flaxseed, the removal of bound phenolics showed little effect on the electrophoretic behavior of the proteins or the protein subunits. PRACTICAL APPLICATIONS: Phenolic-protein and phenolic-lipid-protein interactions may affect the nutritional, physicochemical, and functional properties of isolated proteins from food in flaxseed and soybean.

Impact of extrusion processing conditions on lipid peroxidation and storage stability of full-fat flaxseed meal.

BackgroundThe full-fat flaxseed (Linum usitatissimum L.) meal has obtained relatively new flourished concept as food or feedstuff for the development of healthier products. It provides favorable balance of polyunsaturated, monounsaturated and saturated fatty acids. However, flaxseed meal may be susceptible to oxidation by exposure to various storage conditions which is extremely undesirable and produces toxic compounds to human health. Another consideration in the application of flaxseed meal relates to the presence of anti-nutritional compounds that need to be minimized using appropriate processing method. The present research work was conducted to evaluate the impact of extrusion processing conditions and storage of full-fat flaxseed meal on functional characteristics such as α-linolenic acid content, lipid peroxidation and sensory attributes.MethodsThe raw flaxseed meal was analyzed for cyanogenic glycosides, tannin and mucilage anti-nutritional compounds. Fatty acids composition was quantified by gas chromatography. The meal was extruded at barrel exit temperature (100–140 °C), screw speed (50–150 rpm), feed rate (30–90 kg/h) and feed moisture (10–30 %) for reduction of anti-nutritional compounds. The raw and extruded meals were stored for a ninety-day period under room conditions (20–25 °C). Lipid peroxidation was analyzed by peroxide, free fatty acids, conjugated dienes, total volatiles and malondialdehyde assay. Color, aroma and overall acceptability attributes were evaluated by sensory multiple comparison tests.ResultsThe raw flaxseed meal possessed significant amount of anti-nutritional compounds, lipid and α-linolenic acid contents. The extrusion processing at high barrel exit temperature (140 °C) significantly reduced the cyanogenic compounds (84 %), tannin (73 %) and mucilage (27 %) in the flaxseed meal. The α-linolenic acid content and lipid peroxidation did not significantly change after extrusion processing or during storage at the end of 60 days. Fluctuations in sensory attributes occurred during storage, but at the end of 90 days, only the extruded samples presented negative effect and showed lowest consumer acceptability.ConclusionsThe present study suggested that extrusion of flaxseed meal at optimum conditions and stored for 60 days did not change the stability of full-fat flaxseed meal and can be used as supplement or ingredient for the production of various healthier products.

Growth performance and quality characteristics of flaxseed-fed broiler chicks

A 42-day study was conducted to evaluate the effect of ground full-fat flaxseed supplementation on growth performance, carcass characteristics, α-linolenic acid (ALA), linoleic acid (LA) and organoleptic characteristics of broiler meat. A total of 200 one-day-old broiler chicks were randomly allocated to five experimental groups and were fed isoenergetic and isonitrogenous diets containing flaxseed at 0%, 2.5%, 5.0%, 7.5% and 10%. Flaxseed supplementation did not affect the weekly body weight of broiler chicks during the first three weeks, but thereafter it reduced significantly with increasing levels of flaxseed in the diets. Birds fed on 10% flaxseed showed a reduction of 10.08% in body weight as compared to the control group. Diets containing 5.0–7.5% flaxseed resulted in significantly lower weight gain, higher feed conversion ratio, energy efficiency ratio and lower protein efficiency ratio as compared to control and 2.5% flaxseed diets. The carcass characteristics data indicated a little variation in the evisceration rate and giblet among treatment groups, but the breast yield was significantly higher in control than flaxseed groups. The protein, fat and ash content of broiler meat were not affected with the level of flaxseed in the diets. However, the inclusion of flaxseed in the diets significantly increased the ALA in the breast and thigh tissues with no difference in the organoleptic quality of meat.

오메가 3계열 지방산을 함유하는 사료의 급여가 육용 토종닭 계육 내 지방산 조성에 미치는 영향 탐색

본 실험은 부존사료자원을 토종닭에 이용하여 EPA 및 DHA와 같은 <TEX>${\omega}-3$</TEX>계 다가불포화지방산을 함유하는 고품질 계육을 생산하기 위한 목적으로 실시하였다. 45일령의 재래종 암수탉을 공시하여 20일간 사양실험을 수행하였다. 일반시판사료를 사용한 대조구와 임자박 10% 첨가구(PM), 임자박 10% 및 아마종실 5% 첨가구(PM+FS), 임자박 10%, 아마종실 5% 그리고 어유 1% 첨가구(PM+FS+FO)로 나누어, 4처리 3반복 그리고 반복당 20수씩 총 240수를 공시하였다. 실험 종료 시체중, 증체량, 사료섭취량 및 사료 요구율 등 생산성과 관련된 항목에서는 처리간에 큰 차이는 관찰되지 않았다. 대조구에 비해 모든 시험구에서 산패도 수치가 약간 높게 나타났으나 처리간에 유의차는 인정되지 않았다. 계육 내의 지방산 조성을 조사한 결과에서 <TEX>${\omega}-6$</TEX>계 불포화지방산의 비율은 처리간에 큰 차이가 관찰되지 않았다. 임자박, 아마종실 및 어유 첨가에 의해 <TEX>${\omega}-3$</TEX>계 불포화지방산(C18:3 <TEX>${\omega}-3$</TEX>, EPA, DHA)의 비율이 증가하는 경향 및 유의하게 증가하는 결과가 나타났다. 특히 EPA와 DHA는 어유를 첨가한 PM+FS+FO구에서 다른 구와 비교하여 유의하게 증가하였다. 본 연구의 결과, 임자박, 아마종실 및 어유를 일반시판사료에 적정량 혼합 급여하는 사료적 처리를 통해 재래종 닭에서 조직 내 총 <TEX>${\omega}-3$</TEX>계 불포화지방산 및 DHA 함량을 증가시킬 수 있었으며, 이렇게 생산된 고부가가치 계육이 <TEX>${\omega}-3$</TEX>계 불포화지방산의 좋은 공급원이 될 수 있을 것으로 사료되었다. Estimations were made of oxidative susceptibility and fatty acid composition of edible meats of native chickens fed various dietary sources containing <TEX>${\omega}-3$</TEX> polyunsaturated fatty acids (PUFA). A total of 240 Korean native chickens were divided into 4 groups, placed into 3 replicates per group, and were fed a commercial diet (Control) or one of the three experimental diets containing 10% perilla meal (PM group), 10% perilla meal-5% full fat flaxseed (PM+FS group), or 10% perilla meal-5% full fat flaxseed-1% fish oil (PM+FS+FO group) for 20 days. Final body weight, weight gain, feed intake, and feed conversion rate among the groups were not significantly different. Dietary treatments did not affect the relative weights of liver, abdominal fat, and breast muscle. The leg weight was increased from the feeding of <TEX>${\omega}-3$</TEX> PUFA sources. The TBA reactive substance in the edible meat was not different with the dietary treatments. The total <TEX>${\omega}-3$</TEX> PUFA in chickens that were fed diets containing <TEX>${\omega}-3$</TEX> PUFA sources increased compared to that of the control. The level of longer chain <TEX>${\omega}-3$</TEX> PUFAs, such as C20:5 <TEX>${\omega}3$</TEX> and C22:6 <TEX>${\omega}3$</TEX> in the PM+FS+FO group, was much higher than that of the others. The addition of local ingredients, such as perilla meal with conventional sources, could be used to obtain value-enhanced meat by enhancing <TEX>${\omega}-3$</TEX> PUFA.

Effects of method of barley grain processing and source of supplemental dietary fat on duodenal nutrient flows, milk fatty acid profiles, and microbial protein synthesis in dairy cows

The objective of this study was to determine the interactive effects of barley grain processing and source of supplemental fat on ruminal nutrient digestion, duodenal nutrient flow, microbial protein synthesis, and milk fatty acid (FA) profiles in dairy cows. Four Holstein cows (656±28kg of BW; 80±12d in milk) fitted with permanent ruminal and duodenal cannulas were used in a 4×4 Latin square design with a 2×2 factorial arrangement of experimental treatments, in which barley was either dry-rolled (DRB) or pelleted (PB) and supplemented with full-fat canola seed (canola) or full-fat flaxseed (flaxseed). Cows fed DRB consumed 1.8 to 3.5kg/d more dry matter compared with those fed PB. Mean ruminal pH was lower and the duration (min/d) and area (pH × min) of total ruminal acidosis (RA; ruminal pH <5.8), mild RA (5.8 >ruminal pH >5.5), and severe RA (5.5 >ruminal pH >5.2), and the duration of acute RA (ruminal pH <5.2) were greater in cows fed PB compared with those fed DRB. Milk yield was unaffected by diet. Milk fat content and yield were greater in cows fed DRB than in cows fed PB. Cows fed PB had greater duodenal flows of C18:1 trans, C18:1 cis, C18:2 trans-9,trans-12, C18:2 trans-9,cis-12, C18:2 trans-11,cis-15, and C18:2 trans-10,cis-12 compared with those fed DRB. Duodenal flows of C18:1 cis, C18:1 trans, total C18:1, C18:2n6, C18:2 cis-9,trans-11, C18:2 trans-10,cis-12, C18:2 trans-11,trans-13, total conjugated linoleic acid (CLA), and C18:3n3 were greater in cows fed flaxseed compared with those fed canola. Milk concentrations of C18:3n3 and total CLA tended to be greater in cows fed PB compared with those fed DRB. When compared with cows fed canola, milk concentrations of C18:2 trans-11,cis-15, C18:2 cis-9,trans-11, C18:2 trans-11,trans-13, total CLA, and C18:3n3 were greater in cows fed flaxseed. Ruminal digestion of neutral detergent fiber and acid detergent fiber was greater in cows fed DRB compared with those fed PB. Duodenal flow of ammonia-N tended to be greater in cows fed PB compared with those fed DRB. Duodenal flow of nonammonia nonmicrobial N was greater in cows fed flaxseed compared with those fed canola; however, barley grain processing did not affect duodenal nonammonia nonmicrobial N flow. Duodenal flow of microbial nonammonia N and microbial efficiency were not affected by diet. In summary, barley grain processing and source of fat altered ruminal FA biohydrogenation, and this was reflected in changes in duodenal FA flow and milk FA profiles; however, ruminal microbial protein synthesis was unaltered.