Fruit Juice Extraction Research Articles

Influence of fractionation of polyphenols by membrane chromatography on antioxidant, antimicrobial and proliferation-inhibiting effects of red fruit juices

Interest in fruit juice extracts as nutraceuticals is constantly increasing due to their health-beneficial properties, mainly caused by polyphenols. However, the correlation between the various effects of fruit juice extracts and their individual composition, including anthocyanins and copigments, is unknown. Therefore, in the present study, eight red fruit juice extracts were prepared using XAD-7 column chromatography, followed by fractionation and identification of the different compounds as well as characterization of their health-promoting effects. The fruit juice extract of pomegranate, chokeberry, and cranberry showed the highest antimicrobial potential against food-borne pathogens. The highest antioxidant and cell proliferation-inhibiting potential was also found in the pomegranate extract. It can be assumed that pomegranate extracts, which are rich in copigments, especially hydrolyzable tannins, are suitable natural antioxidants and antimicrobial agents. Pomegranate extracts could be used as nutraceuticals or natural preservatives.

From the chokeberry fruit products and by-products to health-promoting effects through multifaceted in vitro bioactivity evaluation and molecular docking studies

BACKGROUND: Polyphenolic-rich chokeberry extracts and juice could provide health benefits in humans. Moreover, by-products after processing stand out as a potential new source of valuable compounds. OBJECTIVE: The research aimed to evaluate in vitro bioactivity of chokeberry fruit extract, juice, and waste extract (material remaining after juice processing) based on their chemical composition and molecular docking analysis. METHODS: Spectrophotometric methods were used to determine the phenolics, anthocyanins, flavonoids, and proanthocyanins content in freeze-dried samples. Additionally, individual anthocyanins, flavonoids, and sugars were identified through HPLC analysis. The antioxidant capacity was tested using two in vitro methods and assessing antimicrobial activity. The effects of tyrosinase and acetylcholinesterase inhibition were examined using spectrophotometric methods. Molecular docking analysis identified the interaction of chokeberry components with the active sites of these enzymes. RESULTS: The waste extract exhibited the most robust antioxidant activity within the DPPH system. While bactericidal activity was absent across all chokeberry preparations, they demonstrated inhibitory effects on specific microbiological strains relevant to the gastrointestinal tract. Results revealed the chokeberry products’ ability to inhibit tyrosinase and acetylcholinesterase. Molecular docking confirmed the strong interactions between chokeberry compounds and these enzymes. CONCLUSION: The results indicate the potential of chokeberry products and by-products as valuable resources for pharmaceutical applications.

Effects of Various Steam Explosions on the Efficiency, Yield, and Loss in a Hydraulic Press Extractor of Fruit Juice

High post-harvest losses occur as a result of the fruits rapid decline in quality after harvest. Using a steam exploded simple juicer is one approach to make up for this loss. The aim of this study was to optimise the processing conditions of a manually operated hydraulic press for the extraction of fruit juice. To measure extraction yield, efficiency, and principle extraction loss, freshly selected fruits (orange, pineapple, and watermelon) were sliced to 20, 40, 60, and 80 mm, steam-conditioned for varying lengths of time (5, 10, and 15 minutes), and loaded onto the machine at various rates (50, 75, and 100%). The Response Surface Methodology (RSM) was employed to optimize the procedure. To ascertain the variations in extraction yields using reference extraction methods, a comparative study was also carried out. The impact of the investigated parameters on the extraction procedures were significant (p 0.05). The pineapple was sliced to 71.25 mm, steamed for 12.60 minutes, and loaded at a rate of 50.61% under the optimal conditions of the press, resulting in an extraction yield, machine efficiency, and extraction loss, which were 79.56, 67.48, and 11.96% respectively. When compared to reference extraction methods, the device yielded the highest fruit extraction yields. Thus, the press was an efficient, effective, and industrially appropriate extraction method that minimizes post-harvest losses.

Phytochemical screening and investigation on the antibacterial activity of fermented noni (Morinda citrifolia) fruit juice extracts on selected microorganisms in Makurdi Metropolis

Plant products are generally useful for medicinal purposes, one of which is the noni plant. Noni or pace (Morinda citrifolia L.) fruit juice was extracted using aqueous and methanol solvents, then fermented and screened for the presence or absence of bioactive constituents (phytochemicals) using the modified standard procedures. The major phytochemical constituents revealed were alkaloids, anthraquinones, cardiac glycosides, and flavonoids, phenolic compounds, saponins, tannins, reducing sugar, saponnins, carbohydrate and terpenoids. Bacterial cell viability and MIC values were also determined by turbidity test. The lowest concentration of the extract with clear suspension was considered the MIC values. The lowest concentration of the extract in the post-incubation suspensions which did not harbour any bacterial growth upon spotting on MHA after overnight incubation at 37° C was considered the MBC values. The test was performed in triplicates alongside antibiotics ciprofloxacin (5μg) as a positive control at a concentration of 0.1mg/ml. Antibacterial activity was perfumed against three bacteria (Salmonella typhi, Escherichia coli, and Staphylococcus aureus) by agar-well diffusion method. Activity was observed for both methanolic and aqueous extracts of Morinda citrifolia L. fruit juice against the organisms tested. The extracts had both bacteriostatic and bactericidal activity against the test microorganisms. The Minimum Inhibitory Concentration (MIC) test revealed that the bacterial isolates were inhibited between concentrations of 50mg/ml and 25mg/ml for the methanolic extract, and between 100mg/ml and 50mg/ml for the aqueous extract of the fermented fruit juice. The extract was bactericidal from the range of 100mg/ml to 200mg/ml for both extracts, though with the exception of S. aureus which was bactericidal at 50mg/ml for methanolic extract.

The pectinolytic activity of Burkholderia cepacia and its application in the bioscouring of cotton knit fabric

BackgroundEnzymatic catalysis in different industrial applications is often preferred over chemical methods due to various advantages, such as higher specificity, greater efficiency, and less environmental footprint. Pectinases are a group of enzymes that catalyze the degradation of pectic compounds, the key components of plant middle lamella and the primary cell wall. Pectinases have found applications in multiple industrial processes, including cotton bioscouring, fruit juice extraction and its clarification, plant fiber degumming, paper making, plant biomass liquefaction, and saccharification, among others. The purpose of this study was to taxonomically characterize a bacterial species exhibiting pectinolytic activities and assess its pectinolytic activity qualitatively and quantitatively, as well as test its bioscouring potential. ResultsHere, we report that Burkholderia cepacia, a previously unknown species with pectinolytic activity, exerts such activity comparable to commercially used pectinase enzymes in the textile industry, but requires less temperature for activity. ConclusionQuantitative evaluation of enzyme activity indicates the potential of the bacterial species for use in the bioscouring of cotton knit fabric.

In vitro antibacterial efficacy of Ananas comosus and Vitis vinifera on cariogenic microorganism

Introduction and Aim: Human health concerns are converging, necessitating a concerted effort to find preventive and therapeutic approaches. Fruits and vegetables are the primary sources of essential nutrients and have various bioactive compounds suitable for treating oral diseases in children. In the Indian sub-continent, the most consumed fruit juices are Pineapple - Ananas comosus and Grape - Vitis vinifera because they are easily accessible, cost-effective, and have increased beneficial effects on oral microorganisms. This present study aimed to assess the antibacterial efficacy of fruit juice extracts of A. comosus and V. vinifera on two major cariogenic bacteria, namely Streptococcus mutans and Lactobacillus acidophilus. Materials and Methods: The ethanolic extracts of A. comosus and V. vinifera were prepared, and their antibacterial effects were evaluated by agar well diffusion method against S. mutans and L. acidophilus. Results: The overall mean difference of zone of inhibition of A. comosus and V. vinifera juice extracts was assessed using One way ANOVA. V. vinifera (P-value of <0.01) showed increased antibacterial efficacy against S. mutans and L. acidophilus when compared to A. comosus. Further, V. vinifera juice extract showed a significant zone of inhibition against S. mutans when compared to L. acidophilus. (P = 0.001*) Conclusion: The ethanolic extract of V. vinifera juice extract had better antibacterial efficacy against both microorganisms when compared to A. comosus.

Study of Opuntia humifusa: phytochemical analysis of aqueous fruit extract and green synthesis of Ag2O nanoparticles

Ag2O nanoparticle synthesis was carried out via co-precipitation technique based on using AgNO3 and secondary metabolites present in the aqueous extract of Opuntia humifusa fruit for the first time. The amount of measured total phenol, flavonoid content, flavone, and flavonol content, and anthocyanin were 38.46 μg eq gallic acid mg−1 extract, 8.34 μg eq quercetin mg−1 extract, 0.614 μg eq quercetin mg−1 DW, and 3.1 mmol L−1, respectively. Moreover, the predominant phenolic compound was gallic acid. Catechin and chlorogenic acid were found in both fruit juice and fruit aqueous extract. Scanning electron microscopy (SEM) of Ag2O-NPs showed the average particle size was 10.58 nm. Scanning electron microscopy, X-ray diffraction Spectroscopy, and UV/visible spectrometry also confirmed the synthesis. This study then focuses on IC50 and NO amount differences before and after the synthesis of Ag2O-NPs which were 448.29 and 101.38 μg mL−1 for IC50 and 35.7% and 62.6% for NO, respectively.

Effect of Thermosonication on the Nutritional Quality of Lapsi (Choerospondias axillaris) Fruit Juice: Application of Advanced Artificial Neural Networks.

This study explored the effect of thermosonication on the nutritional properties of lapsi (Choerospondias axillaris) fruit juice. The intent of the present investigation was to process lapsi fruit juice using both thermosonication and thermal pasteurisation and to compare the effects of these treatments on the juice's physicochemical, nutritional, and microbiological qualities. In order to maximise the retention of nutritional properties, enhance juice quality, and boost efficiency, an artificial neural network (ANN) model was also developed to forecast the optimisation of process parameters for the quality of lapsi fruit juice. This study establishes a novel experimental planning method using an ANN to multi-objectively optimise the extraction process and identify the ideal extraction conditions for thermosonication (50, 75, and 100% amplitude at 30, 40, and 50 °C for 15, 30, 45, and 60 min) to augment lapsi juice's nutritional and microbiological properties by improving certain attributes such as ascorbic acid (AA), antioxidant activity (AOA), total phenolic content (TPC), total flavonoid content (TFC), total plate count, and yeast and mould count (YMC). The maximum values for AA (71.80 ± 0.05 mg/100 mL), AOA (74.60 ± 0.28%), TPC (187.33 ± 0.03 mg gallic acid equivalents [GAE]/mL), TFC (127.27 ± 0.05 mg quercetin equivalents [QE]/mL), total plate count (not detected), and YMC were achieved in thermosonicated lapsi juice (TSLJ) under optimal conditions. For AA and TFC, the optimal conditions were 100% amplitude, 40 °C, and 45 min. For AOA and TPC, the optimal conditions were 100% amplitude, 40 °C, and 60 min, and for YMC, the optimal conditions were 100% amplitude, 50 °C, and 60 min. According to the findings, thermosonicated juices have improved nutritional properties, making them an excellent source of bioactive elements for use in both the food and pharmaceutical sectors. According to this study, ANN has been identified as a valuable tool for predicting the effectiveness of lapsi fruit juice extraction, and the application of thermosonication as an approach for lapsi juice preservation could be a potential successor to thermal pasteurisation. This approach can help to minimise or hinder quality degradation while improving the juice's functionality.

Protective Effect of Pomegranate Juice Extract Against Antitubercular Drugs Induced Hepatic Fibrosis

The current study aimed to know the therapeutic efficacy of Punica granatum (fruit juice extract) against anti-TB druginduced hepatic fibrosis in rats. 24 male Wistar albino rats were randomly divided into four groups, with six rats in each. INH at 27 mg/kg B.W., RIF at 54 mg/kg B.W., and PZA at 135 mg/kg B.W. were administered orally to Groups 2, 3 and 4 from days 1 to 28. Group 1 was maintained as normal control, and group 2 was a toxic control (administered anti-TB drugs, p/o). Groups 3 and 4 were administered (p/o) with Enalapril at 5mg/kg B.W. and Punica granatum (fresh juice extract) at 1 ml/rat, respectively, from day 1 to 28. Serum samples were collected on the 14th and 28th to assess various biochemical parameters, and liver samples were collected at the end of the experiment to analyze antioxidant parameters, TGF β1, and histopathological examination. The biochemical parameters showed significant (p<0.05) alterations in AST, ALT, GGT, antioxidant profile (TBARS GSH, GST, GPx and SOD), and expression of TGF β1 levels in group 2 along with collagen deposition and alterations in the histopathology when compared with group 1. There was a significant improvement in treatment groups 3 and 4 at different time intervals. These changes were reversed in groups 3 and 4, which were administered Enalapril and Punica granatum juice extract, respectively.

Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expression.

Introduction: Alkaline pectin lyase is an important enzyme with a wide range of applications in industrial production, It has been widely used in many important fields such as fruit juice processing and extraction, the dyeing and processing of cotton and linen textiles, degumming plant fibers, environmental industrial wastewater treatment, and pulp and paper production. PGLA-rep4 was previously generated as a modified alkaline pectin lyase with high specific activity at pH 11.0°C and 70°C. However, the pre-constructed high-activity pectin lyase expression strains are still difficult to apply in industrial production due to their limited enzymatic activity. We hope to solve these problems by combining modern breeding techniques with high-throughput equipment to rapidly screen alkaline pectin lyase with higher enzymatic activity and lower cost. Methods: We fused the genes encoding PGLA-rep4 and fluorescent protein egfp using a flexible linker peptide and ligated them into a temperature-sensitive plasmid, pKD46. The constructed screening plasmid pKD46-PGLA-rep4-egfp was then transformed into an expression host and screened via flow-cytometric cell sorting coupled with UV mutagenesis. Results: Following mutagenesis, primary screening, and secondary screening, the high-expression strain, named Escherichia coli BL21/1G3, was obtained. The screening plasmid pKD46-PGLA-rep4-egfp was eliminated, and the original expression plasmid pET28a-PGLA-rep4 was then retransformed into the mutant strains. After induction and fermentation, pectin lyase activity in E. coli BL21/1G3 was significantly increased (1.37-fold relative to that in the parental E. coli BL21/PGLA-rep4 strain, p < 0.001), and the highest activity was 230, 240U/mL at 144h. Genome sequencing revealed that genes encoding ribonuclease E (RNase E) and diadenosine tetraphosphatase (ApaH) of E. coli BL21/1G3 were mutated compared to the sequence in the original E. coli BL21 (DE3) strain, which could be associated with increased enzyme expression. Discussion: Our work provides an effective method for the construction of strains expressing pectin lyase at high levels.

Anti-Obesity Effects of Punicalagin and Punica granatum Fruit Juice Extract in Adipocytes Differentiated from Human Bone Marrow Mesenchymal Stem Cells

Anti-Obesity Effects of Punicalagin and Punica granatum Fruit Juice Extract in Adipocytes Differentiated from Human Bone Marrow Mesenchymal Stem Cells

ỨNG DỤNG CHẾ PHẨM ENZYME PECTINASE ĐỂ KHAI THÁC VÀ THU HỒI DỊCH QUẢ CHANH LEO CỦA TỈNH CAO BẰNG

Cao Bang is a province with favorable natural conditions for the development of passion fruit trees (Passiflora edulis). Passion fruit is rich in nutritional values, such as protein, sugar, vitamins, minerals, phenolic acids, and flavonoids. The addition of pectinase enzyme preparation has the effect of breaking down the cell wall of passion fruit flesh, contributing to increasing the yield of fruit juice. The objective of this study is the application of pectinase enzyme preparations to exploit and recover passion fruit juice from Cao Bang province. The study has determined extraction and recovery of passion fruit juice was developed, with the technological parameters being the concentration of pectinase enzyme preparation used as 0.5%, the hydrolysis temperature of 39 oC, the hydrolysis time as 300 minutes, in this condition the recovery efficiency of passion fruit juice was 89.04%. Passion fruit juice has a dark orange yellow color, a very characteristic aroma, a sweet taste mixed with a slightly sour taste, a homogeneous state, a soluble solids content of 12.75 ± 0.25 oBx, and a pH of 2.81 ± 0.02.

Procena antimikrobne aktivnosti soka i ekstrakta crne ribizle (Ribes nigrum L.) sorte Čačanska crna

Aim. This study aimed to evaluate and quantify the antimicrobial activity of lyophilized fruit juice (BCLJ) and waste extract (BCLW) obtained from the black currant (Ribes nigrum L.) variety Čačanska crna. Materials and method. The study was conducted using four Gram (+) (Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis) and five Gram (-) bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Proteus mirabilis, Enterobacter aerogenes) as well as one yeast (Candida albicans). Cyanidin-3-O-glucoside, delphinidin-3-O-rutinoside and delphinidin-3-Oglucoside present in black currant were used as standards, so the second aim was to determine their influence on the total antimicrobial activity. Results. The tested samples showed moderate antimicrobial activity. The inhibitory effect of BCLJ was shown on all Gram (+) bacteria (B. cereus, E. faecalis, S. aureus), apart from L. monocytogenes, for which the extracts were not effective. It was noted that BCLJ did not suppress the growth of Gram (-) bacteria. Black currant waste extract on the other hand was shown to be efficient on Gram (+) as well as on Gram (-) bacteria. The results of minimum inhibitory concentrations MIC (MFC) of BCLJ and BCLW were 100 mg/mL and MBC was higher than 100 mg. The MIC/MBC (MFC) of standards were 0.13 - 0.5 mg/mL. Conclusion. Results indicate that these black currant lyophilizates might be potentially used as antimicrobial agents.

Use of concentrated fruit juice extracts to improve the antioxidant properties in a soft drink formulation

Three juices obtained respectively from pomegranate, prickly pear and orange fruit were separately concentrated using an innovative technique, that allow to obtain a final product with high content of bioactive compounds and elevate antioxidant activity. The concentrated juices were employed in the formulation of a soft drink to improve its bioactive properties. Despite a low amount of each concentrated juice was used, the experimental soft drink obtained evidenced a high content of total polyphenols. The antioxidant activity detected was similar to that of tea commonly considered a healthy beverage with strong radical scavenging activity. The sensory analysis of the soft drink showed a consumers' liking score potentially promising for market acceptance. In addition, the experimental drink produced was evaluated for its stability by simulating domestic storage and from the results obtained is possible to assess that a good antioxidant activity was maintained even after 5 days of conservation. Therefore, the present work, stimulates the use of high-quality concentrated juices as food ingredient in order to improve the functional properties of soft drinks.

Engineering magnetic nanobiocatalytic systems with multipurpose functionalities for biocatalysis, biotechnology and bioprocess applications

Enzymes are high-performance natural biological catalysts with wide-ranging applications in agricultural, medical, food and environmental sectors. Nevertheless, lack of efficient recovery, reusability, and high cost of the soluble form of enzymes are the most daunting challenges rendering biocatalytic systems inadequate for industrial exploitation. In order to deal with these inadequacies, immobilization appears to be a prodigious approach for enhancing the stability and catalytic efficiency of enzymes, as well as enabling their separation and reusability in continuous reaction batches. Among different nanostructures, magnetic nanomaterials have garnered supreme interest as support matrices for biomolecules and enzymes immobilization because of their substantial surface area, larger surface-to-volume ratio, modifiable surface, and adjustable surface particle size, stability, and high mass transferring ability. In addition, they can be quickly recovered from the complex reaction system by a simple external magnetic field. Magnetic nanoparticles incorporated biocatalysts demonstrated a broad-working temperature and pH profile and augmented storage and thermal stabilities compared to their native derivatives. This paper provides a recent and state-of-the-art overview of the development and application of multifunctional magnetic nanobiocatalytic systems for an array of biotechnological purposes. In the first half, the development, functionalization, and use of nanostructured magnetic materials as enzyme immobilization supports are delineated. Then the prospective applications of magnetic nanobiocatalytic systems in different industrial sectors, including wastewater treatment, biodiesel and butanol production, hydrolysis of lignocellulosic biomass, glucose monitoring, fruit juice extraction and clarification, and synthesis of non-natural benzylisoquinoline alkaloid are comprehensively vetted with representative examples. Finally, the conclusive points and future prospects in this evolving field are also directed.

Citrus maxima (Burm.) Merr. fruit juice and peel extract mediated synthesis of silver nanoparticles (Ag NPs) and their applications as antimicrobial agents and plant growth enhancers

Green synthesis of metal nanoparticles (NP) has emerged as a recent trend in green chemistry. However, their potential in the field of agriculture is yet underexplored. In this study, silver nanoparticles (Ag NPs) were synthesized by using the fruit juice and peel extract of Citrus maxima (Burm.) Merr. as a reducing and capping agent. The nano-primed seeds of Zea mays, Glycine max, and Cicer arietinum showed enhanced germination rate up to 90% as compared to the respective control (30-60%) and also enhanced plant growth. The HRTEM images of fruit-mediated Ag NPs showed well-dispersed particles. The XRD diffraction pattern confirmed the crystalline nature of Ag NPs with fcc lattice points of 101, 111, 200, 220, and 311 with an average crystal size of 19.1275±1.73 nm. On the other hand, the peel-mediated Ag NPs exhibited a size range of 10-20 nm with average crystal size of 19.09±1.71 nm. The FTIR spectra confirmed the presence of ʋC=C-H, ʋCH2, ʋC=O (stretch), ʋC=C (stretch), ʋC-H (bend), ʋC-O (bend) in the Ag NPs synthesized by the fruit and peel extract. The Ag NPs also showed potential antimicrobial effects against both Gram-positive (Staphylococcus aureus) and negative bacteria (Klebsiella pneumonia).

Synthesis, characterization and evaluation of biological properties of selenium nanoparticles from Solanum lycopersicum

Synthesis of nanoparticles by green synthesis has a large number of biomedical applications worldwide. In this study, Selenium Nanoparticles (SeNPs) were synthesized by using sodium salt of selenium and Solanum lycopersicum (tomato) fruit juice and seeds extract. The plant extracts were used as a reducing agent in ratio 1:4 i.e. sodium selenite salt (Na2SeO3). SeNPs were characterized by UV–visible spectrophotometry, FTIR and Zeta Sizer analysis. The UV-graphs indicated the highest peak of absorbance at 350 nm. Whereas, FTIR analysis of SeNPs indicated absorbance bands at 3262.35–1633.72 cm−1. Zeta sizer analysis showed the average size of SeNPs for Fruit juice extract as 1020 d.nm. with PDI 0.432. In case of seeds extract, average size was 1155 d.nm. with PDI 0.761; and the PDI value for both extracts showed polyderse nature of these NPs. SeNPs possessed significant antimicrobial activity against selected strains of E. coli, S. aureus, M. luteus, S. enterica, B. subtilis, K. pneumoniae and P. aureginosa. The α-amylase inhibitory assay of these SeNPs indicated that they had antidiabetic role with IC50 value 24.4642 µg/mL. The DPPH assay showed that SeNPs of Solanum lycopersicum have antioxidant activity with IC50 value of 20.7398 µg/mL.

L’acérola, un fruit exotique riche en vitamine C contient une LTP allergénique

Aim of the studyAcerola is a tropical fruit 20 to 30 times richer in vitamin C than oranges. Acerola is found in tablets or powders commercially available for their richness in vitamin C and also in the form of juice or as additives in compotes and fruit juices. Besides a previous report on the existence of an IgE cross-reactivity of an unidentified acerola protein with Hev b 6 from latex no studies on acerola allergens have been performed. Patients and methodsAcerola proteins were extracted from fresh fruit, commercial juice and a vitamin C-enriched powder. Allergens were studied by an allergomics analysis, including IgE immunoblots, using sera from documented patients followed by protein identification using mass spectrometry. ResultsIgE Immunoblots performed with patients sensitized to LTPs, showed IgE reactivities in low molecular mass corresponding to LTPs. In contrast, no allergenic gibberellin-regulated protein (GRP), PR10 and profilin were revealed. The presence of LTP was confirmed by the use of specific anti-Pru p 3 antibodies and their identification in fresh fruit and commercial juice extracts by mass spectrometry that further unravelled several putative allergens. LTP was also detected in vitamin C-enriched powder. ConclusionAcerola fruit and juice as well as derived products containing acerola carries a risk for LTP sensitized patients.

A Novel Endo-Polygalacturonase from Penicillium oxalicum: Gene Cloning, Heterologous Expression and Its Use in Acidic Fruit Juice Extraction.

An endo-polygalacturonase (endo-PGase) exhibiting excellent performance during acidic fruit juice production would be highly attractive to the fruit juice industry. However, candidate endo-PGases for this purpose have rarely been reported. In this study, we expressed a gene from Penicillium oxalicum in Pichia pastoris. The recombinant enzyme PoxaEnPG28C had an optimal enzyme activity at pH 4.5 and 45°C and was stable at pH 3.0-6.5 and < 45°C. The enzyme had a specific activity of 4,377.65 ± 55.37 U/mg towards polygalacturonic acid, and the Km and Vmax values of PoxaEnPG28C were calculated as 1.64 g/l and 6127.45 U/mg, respectively. PoxaEnPG28C increased the light transmittance of orange, lemon, strawberry and hawthorn juice by 13.9 ± 0.3%, 29.4 ± 3.8%, 95.7 ± 10.2% and 79.8 ± 1.7%, respectively; it reduced the viscosity of the same juices by 25.7 ± 1.6%, 52.0 ± 4.5%, 48.2 ± 0.7% and 80.5 ± 2.3%, respectively, and it increased the yield of the juices by 24.5 ± 0.7%, 12.7 ± 2.2%, 48.5 ± 4.2% and 104.5 ± 6.4%, respectively. Thus, PoxaEnPG28C could be considered an excellent candidate enzyme for acidic fruit juice production. Remarkably, fruit juice production using hawthorn as an material was reported for the first time.

Indian jujube (Ziziphus mauritiana L.) fruit juice extraction using cellulase enzyme: Modelling and optimization of approach by ANN-GA

The Indian jujube is a seasonal fruit that is beared in abundant during February and March in India. The fruit is rich in ascorbic acid and bioactive compounds like total phenolic compounds and antioxidant activity. It is a significant source of revenue for the local tribes. However, due to a lack of scientific documentation and value addition, a substantial amount of fruit is thrown away. As a result, making juice from Indian Jujube pulp will be a cost-effective and straightforward option that will extend its shelf life and aid in its commercialization. A Box–Behnken design was generated with 17 experimental runs was modelled using an artificial neural network by taking independent variables as incubation temperatures (30, 40, and 50 °C), incubation duration (40, 70, and 100 mins) and enzyme concentrations (0.01, 0.055, and 0.1 uM) while taking dependent variables as yield, viscosity, refractive index, total soluble solids, polyphenol, antioxidant activity, and protein content. The model was fitted successfully with a maximum R-value of 0.9997 and a least MSE 0.0978. The weight and bias values obtained by artificial neural network modelling was used by a genetic algorithm to estimate the fitness function, which minimizes dependent variables like viscosity and maximizes other dependent variables. The optimized independent variables obtained were 30 °C incubation temperature, 58 min of incubation duration and 0.1 uM of enzyme concentration with mean and best fitness values of -187.238 and -187.304, respectively. The optimized independent variables were validated by physiochemical experiment, which resulted in juice yield (80.89%), viscosity (3.11 cP), refractive index (1.3549), total soluble solids (14.20 °Brix), polyphenolic content (0.94 g GAE/100 mL), antioxidant activity (82.41%) and protein content (9.53 mg/100 ml). The results revealed that the observed effects of the responses of enzyme-treated were significantly higher than those of the juice without treatment. Therefore, these conclusions highlight the highly effective method of juice extraction with a better statistical approach.