Freshwater Ichthyofauna Research Articles

A Systematic Literature Review on Ichthyofaunal Diversity in Fresh Water Ecosystem, with Special Reference to Maharashtra

The study highlights a review of ichthyofaunal diversity across major rivers in Maharashtra such as the Godavari, Krishna, Tapti, Bhima, and Wainganga etc. Secondary data from various sources, research publications, were analysed. River Godavari had the most studied River. The scope of fish and fisheries in Maharashtra holds significant economic importance, particularly for local livelihoods and food security. Freshwater fish diversity in the state, as studied between 2009 and 2024, The current study deals with the freshwater ichthyofauna recorded and confirmed by different author shows a rich variety with 160 species recorded, spanning 9 orders, 25 families, and 80 genera. The most dominant order was Cypriniform followed by Siluridae. This diversity not only supports the fishing communities but also plays a critical role in meeting the demand for fish, which is a key source of protein for consumers. The review of this data provides valuable insights for fishermen. It also aids the fish industry by offering guidance on sustainable practices, as overfishing and habitat destruction can threaten these species.

Peculiarities of Arctic Ichthyofauna

An audit of the marine and freshwater ichthyofauna of the Arctic has been carried out. According to new data, the ichthyofauna of the Arctic is represented by 4 classes, 33 orders, 103 families, 251 genera and 479 species of cyclostomes and fish (or about 1% of the world fauna), of which 214 species or 45% of the number of species in the Arctic are true Arctic species. Arctic fish are characterized by polymorphism and euryphagy, which are associated with the variable and low food supply of northern water bodies. The ichthyofauna of the Arctic is well represented by taxa with a wide adaptive radiation (genera Coregonus, Salvelinus, Lycodes, etc.) and species with a complex intraspecific structure such as superspecies or species-complex. Another characteristic feature of the Arctic is low endemism of ichthyofauna at the level of families (5%), genera (2%) and species (10%). Possible routes of distribution of whitefish and pike in the water bodies of the North have been discussed.

Estuarine ichthyofauna of Kamchatka: formation conditions, species diversity and ecological characterisation

Species composition of the estuarine ichthyofauna is described for the first time on a regional scale for Kamchatka Territory based on the materials of author’s field studies carried out in 2010–2022, as well as on the analysis of all available information (literature, archival, survey, fishery, etc.). Specific features of the conditions of formation of estuarine ichthyofauna of the region are revealed. Fish species dominating in abundance in Kamchatka estuaries are indicated. The main ecological groups (guilds) of estuarine fishes are outlined. It was found that 1 species of cyclostomes and 60 species of fishes belonging to 44 genera and 23 families can be attributed to the estuarine ichthyofauna of Kamchatka. All these species are, to varying degrees, euryhaline and therefore may utilize individual Kamchatka estuaries as habitats at various stages of the life cycle. The basis of species diversity of the ichthyofauna of Kamchatka estuaries is formed by marine fishes (at least 34 species), the second in importance are anadromous species (1 species of cyclostomes and 14 species of fishes), the minimum contribution belongs to freshwater resident fishes (13 species), which are found only in estuaries of some rivers of the region. Fish that can be referred to the ecological group of “solely estuarine” (i. e., those whose full life cycle takes place in estuaries) are probably absent in Kamchatka. The maximum species diversity of fish communutues is observed either in big Kamchatka estuaries that are largely open to the sea or in river basins with a diverse freshwater ichthyofauna. Environmental conditions in such estuaries are more favorable for the migrations and habitat of marine fish species (mainly coastal ichthyocene). At the same time, all anadromous or some freshwater euryhaline species may also use these same estuaries as temporary habitats at certain stages of the life cycle. Fish species diversity is significantly higher in the estuaries of the eastern coast of Kamchatka, which is explained by the milder climatic conditions of this area (compared to the coast of the Sea of Okhotsk), as well as by the greater typological diversity of estuaries. A separate section summarizes the currently available information on the biology and ecology of all species of fishes and fish of Kamchatka estuaries, which is presented in the form of brief species sketches (including, external view at individual stages of the life cycle; key morphological characters; maximum size and age; distribution over the territory of the region; habitat specifics, lifestyle and migrations; peculiarities of reproduction and development; feeding preferences; abundance in the region, commercial or economic importance).

Peixes de afluentes do Rio Branco, Município de Caracaraí, Estado de Roraima, norte do Brasil

Abstract The Amazon has the richest freshwater ichthyofauna of the planet, with tens of new species being described annually. Although studies on Amazonian fish in the literature are increasingly common recently, there are still significant gaps concerning primary data on the ichthyofauna. One such gap is the state of Roraima and its main drainage, Rio Branco. There is a single book published in 2007 on fishes of Rio Branco, and although it presents a rather complete list of over 580 species known until then, the information is now a little outdated and many species found in some of its affluents are not listed in the book. Due to the scarcity of published data on the fish diversity of Roraima and taking into account that ichthyofaunal surveys are important tools towards freshwater conservation, we carried out an inventory of the ichthyofauna in the region of Caracaraí (RR) in 18 sampling sites including seven affluents of both left and right margins of Rio Branco. We recorded a total of 64 species of 41 genera and 18 families of five orders that occur in the Neotropical region, representing more than 11% of the species previously registered in the book for the entire Rio Branco basin. Twelve species were added to the list presented in the book, with four representing first records for the basin, one of them a new record for Brazil. Two of the 64 species are putative new taxa in need of further taxonomic studies. The order with the highest diversity was Characiformes (40 species), with highlights for the family Characidae (24 of these species), followed by Cichliformes (11 species) and Siluriformes (9 species). The richest collecting sites had 23 species, and the least rich site had only two species. Hyphessobrycon bentosi and Nannostomus marginatus occurred in more than 60% of the sites. There has been a significant difference in the exclusive ichthyofauna from affluents of both margins of Rio Branco, with the number of exclusive species in left margin tributaries approximately five times higher. Results presented herein complement data from the literature regarding the still poorly known ichthyofauna from Roraima.

Biogeographic patterns of the freshwater fishes from the state of Espírito Santo, eastern Brazil

ABSTRACT Biogeographic studies offer valuable insights into the origin, distribution and extinction of biodiversity. As such, this study aims to identify patterns of diversity and distribution of freshwater ichthyofauna in the state of Espírito Santo, to delineate biogeographic units and to propose a hypothesis of area relationship. A database containing information on 123 fish species occurrences was compiled to conduct analyses of species richness interpolation, bioregionalization, sampling effort interpolation, endemism, and a parsimony analysis of endemism. The sampling effort in Espírito Santo is uneven, with higher representativeness of sampling sites in the highlands. Six freshwater ichthyofauna bioregions were identified, along with three major areas of increased species richness, two consensus areas of endemism and three groups of basins. The central basins of the state are characterized by a marked presence of endemic species. The southern basins also stand out as an area of endemism and density of species typical of mountainous environments, such as loricariids; they are also characterized by a significant faunal overlap with the Rio Paraíba do Sul basin. The results of this biogeographic evaluation reinforce the perception that the watersheds of the state of Espírito Santo have a diverse and characteristic freshwater fish fauna, with well-defined biogeographic units.

Geomorphological habitat type drives variation in temporal species turnover but not temporal nestedness in Amazonian fish assemblages

Explaining the mechanisms underlying spatial and temporal variation in community composition is a major challenge. Nevertheless, the processes controlling temporal variation at a site (i.e. temporal β‐diversity, including its turnover and nestedness components) are less understood than those affecting variation among sites (i.e. spatial β‐diversity). Short‐term temporal turnover (e.g. throughout an annual cycle) is expected to correlate positively with seasonal environmental variability and landscape connectivity, but also species pool size (γ‐diversity). We use the megadiverse Amazonian freshwater ichthyofauna as a model to ask whether seasonality and landscape connectivity drive variation in temporal species turnover among geomorphological habitat types, while accounting for between‐habitat variation in γ‐diversity. We collected 11 397 fish representing 260 species during a year‐long sampling program from an area containing the lowland Amazon's four major geomorphological habitat types: rivers, floodplains, terra firme streams, and shield streams. River‐floodplain systems exhibit strong but predictable seasonality (via a high‐amplitude annual flood pulse), high connectivity, and high species richness with many rare species. Terra firme and shield streams exhibit low seasonality, low connectivity, and low species richness with proportionally fewer rare species. Based on these parameters we predicted that river‐floodplain systems should have higher temporal turnover than stream systems. Using a null model approach combined with β‐deviation calculations, we confirmed that rivers and floodplains do exhibit higher turnover (but not nestedness) than terra firme and shield streams, even when controlling for the potentially confounding effect of higher species richness in river‐floodplain systems. All habitats exhibit low temporal nestedness, indicating that short‐term changes in community composition result primarily from temporal species turnover. Our results provide a timely reminder that efforts to conserve the Amazon's threatened aquatic biodiversity should account for the distinct temporal dynamics of habitat types and variation in hydrological seasonality.

Molecular phylogenetics of the fresh water sleepers Odontobutis (Gobiiformes: Odontobutidae) and its implications on biogeography of freshwater ichthyofauna of East Asia

The genus Odontobutis is a group of freshwater fishes endemic to East Asia. Phylogenetic relationships among the Odontobutis species have never been fully tested due to incomplete taxon sampling and that molecular data have not been collected in many Odontobutis species. In the present study, we sampled 51 specimens from all known eight Odontobutis species with two outgroups (Perccottus glenii and Neodontobutis hainanensis). We collected sequence data of 4434 single-copy nuclear coding loci using gene capture and Illumina sequencing. A robust phylogeny of the Odontobutis with many individuals for each species was built, supporting the current taxonomy that all extant Odontobutis species are valid. The two species from Japan (O. hikimius + O. obscurus) formed an independent clade sister to the “continental odontobutids”, whereas the species from southern China (O. sinensis + O. haifengensis) separated from the rest species of the genus. Surprisingly species from the lower reaches of the Yangtze River (O. potamophilus) was more closely related to species from the Korean Peninsula and northeastern China than to the middle reaches of the Yangtze River, such that their relationship was ((O. sinensis + O. haifengensis)(O. platycephala + (O. yaluensis + (O. potamophilus + O. interruptus)))). Divergence time among the Odontobutis was estimated using 100 most clock-like loci and three fossil calibration points. The crown group of the Odontobutis was estimated at 9.0 Ma during the late Miocene (5.6–12.7 Ma, 95% HPDs). Ancestral range of the genus was reconstructed using Reconstruct Ancestral States in Phylogenies (RASP) and BioGeoBEARS. The result suggested that the common ancestor of modern Odontobutis probably was distributed in Japan, southern China or the Korean Peninsula. A series of geographical events in East Asia since the late Miocene, such as the opening of the Japan/East Sea, rapid uplift of the Tibetan Plateau and climate change in the northern region of the Yellow River might account for diversification and current distribution pattern of the Odontobutis.

Ecological Features and Opportunities for Economic Use of the Solovetsky Archipelago Freshwater Ichthyofauna

The species composition and taxonomic diversity of the Solovetsky Archipelago freshwater ichthyofauna were revealed, and a description of the current state of the fish was given. The belonging of fish to freshwater faunistic complexes and ecological groups (nature of feeding and mode of natural reproduction) was examined. Currently, 14 species of fish were found in the inland waters of the Solovetsky Islands, including local and alien species with varying degrees of acclimatization effect. A possible annual catch of freshwater fish has been established, which could be considered optimal. If it is necessary to organize freshwater aquaculture, pasture cultivation in the lakes of the Solovetsky Archipelago of the whitefish complex seems promising. It is based on the collection and fertilization of eggs at temporary fish breeding stations, its subsequent incubation at fish farms and the release of reared juveniles into feeding lakes.

Current knowledge of New Caledonian marine and freshwater ichthyofauna, SW Pacific Ocean: diversity, exploitation, threats and management actions

Current knowledge of New Caledonian marine and freshwater ichthyofauna, SW Pacific Ocean: diversity, exploitation, threats and management actions

Cytogenetic and Molecular Characterization of Eigenmannia aff. desantanai (Gymnotiformes: Sternopygidae): A First Report of System of Sex Chromosomes ZW1W2/ZZ in Gymnotiformes.

Gymnotiformes a monophyletic group of fish endemic to the Neotropics, represent an important component of the freshwater ichthyofauna that presents relevant taxonomic problems. Thus, in view of the morphological complexity involving Eigenmannia (Gymnotiformes) fish species, this study aimed to characterize Eigenmannia aff. desantanai of the upper Paraguay River basin through cytogenetic and molecular analyses, to help in the correct identification and delimitation of species. This study reports a multiple sex system of the type ZW1W2/ZZ, with 2n = 31 for females and 2n = 30 for males. A single pair of chromosomes carrying the nucleolar organizing regions (NORs) was detected. The heterochromatin was colocated in NOR sites and mainly located in the centromeric regions of chromosomes. Besides that, individual sequences COI from the specimens of E. aff. desantanai were obtained, totalizing three haplotypes. The distance p between the haplotypes in E. aff. desantanai, ranged from 0.2% to 7.1%. Species delimitation tests indicated the existence of two possible operational taxonomic units of E. aff. desantanai. Thus, this study reports a new multiple sex system in Gymnotiformes and these specimens previously identified as E. aff. desantanai may belong to two distinct species.

Recent Data on the Distribution of Freshwater Ichthyofauna in Albania

Abstract In this article, we provide an updated list of freshwater fishes in Albania for each sub-basin. The distribution data and taxa-lists presented are based on a literature review up to October 2022 and intensive fieldwork conducted in the last 15 years. Distributional records of 101 fish species (deriving from 25 families) in 11 different sub-basins are reported. There are new records that include native, alien and translocated fish species. The distinctiveness of Albanian freshwater fishes is highlighted while defining a specific ichthyological region for the whole Balkan region. The freshwater fish fauna of Albania represents a special value of the national heritage, especially due to its diversity and a high degree of endemicity. This is mainly due to the complex geological and climatic conditions, and the location between the Adriatic and Ionian Seas on the west and the continental areas of the Balkans on the other side, which have allowed different colonization from outside the area and long periods of speciation. Eleven hydrographic basin units are analyzed for species composition, evolutionary patterns, and ecological features. This review brings the number of Albanian freshwater fish taxa to 101, with 20 species added from the previous 1995 inventory and several species deleted due to taxonomic changes.

Covariation of taxonomic and functional facets of β-diversity in Chilean freshwater fish assemblages: Implications for current and future processes of biotic homogenization.

The biodiversity of assemblages that experience the introduction and extinction of species may lead to responses in two important facets: The taxonomic and functional diversity. The way in which these facets are associated may reveal important implications and consequences for the conservation of those assemblages. Considering the critical situation of freshwater fishes in continental Chile (30° - 56° S), we analyzed how the taxonomic (TDβ) and functional (FDβ) facets of β-diversity, and their components of turnover and nestedness, are associated. We evaluated changes in β-diversity (ΔTDβ and ΔFDβ), turnover (ΔTDtur and ΔFDtur), and nestedness (ΔTDnes and ΔFDnes) in 20 fish assemblages from their historical (pre-European) to current composition. We also simulated future trends of these changes, assuming that native species with conservation issues would become extinct. Our results show that the fish assemblages studied are in a process of loss of β-diversity, both in taxonomic and functional facets (ΔTDβ = -3.9%; ΔFDβ = -30.4%); also, that these facets are positively correlated in the assemblages studied (r = 0.617; P < 0.05). Both components showed by loss in nestedness (ΔTDnes = -36.9%; ΔFDnes = -60.9%) but gain in turnover (ΔTDtur = 9.2%; ΔFDtur = 12.3%). The functional β-diversity decreased more than the taxonomic (ΔFDβ > ΔTDβ), which was caused chiefly by six exotic species of Salmonidae, whose geographical spread was wider and that at the same time shared several morpho-functional traits. Our forecasts, assuming an intensification in the extinction of Endangered and Vulnerable native species, indicate that the process of homogenization will continue, though at a lower rate. Our study shows that the freshwater ichthyofauna of continental Chile is undergoing biotic homogenization, and that this process involves the facets of taxonomic and functional β-diversity, which are show high correlation between historical and current compositions. Both facets show that process is influenced by nestedness, and while turnover contributes to differentiation (both taxonomic and functional), its importance is overshadowed by nestedness.

DNA barcoding detects resurrected taxon Giuris laglaizei (Sauvage 1880) in Sulawesi, Indonesia: Bolano Sau Lake payangka phylogeny, phenotypic characters and implications for Giuris spp. conservation

Background: The freshwater ichthyofauna of Wallacea is diverse and understudied. A baseline survey of Bolano Sau Lake in Parigi Moutong District, Central Sulawesi Province, Indonesia in 2019 found an eleotrid goby (local name payangka) with characters conforming to the genus Giuris, long considered monophyletic as G. margaritacea/G. margaritaceus but recently found to comprise at least eight species. This study focused on the molecular (DNA barcoding) identification and phenotypic characters of the payangka. Methods: Payangka samples were collected from August to December 2019 in collaboration with local fishermen, weighed and measured, and preserved in 75% ethanol. Length, weight, sex (n=111) and 17 morphometric characters/six meristic counts (n=42) were recorded. DNA barcoding was performed on a fin clipping preserved in 96% ethanol. Homologous nucleotide sequences were obtained from public (GenBank and BOLD) databases, analysis conducted in MEGA X, and phylogenetic trees edited in the Interactive Tree of Life (iToL). Results: Within the deeply divided Giuris clade, the payangka sequence resolved into a sub-clade identified as Giuris laglaizei (Sauvage 1880), a recently resurrected taxon, based on a sequence provided by Philippe Keith. The length-weight relationship (L = 0.0087∙W3.162) indicated mildly allometric positive growth. Size distribution differed significantly between male and female fish with significantly larger mean size of males (13.56 cm) than females (11.62 cm). The meristic formula was: D VI-I,8 A I,8 P 13 V I,5 C15. Phylogenetic analysis indicated four Giuris species in wetlands around Tomini Bay and five in Sulawesi. Conclusions: This first record of G. laglaizei in Indonesia advances knowledge of Wallacean and Indo-Pacific Gobiiformes biogeography and highlights the need for a revision of the conservation status of the taxa currently grouped under Giuris margaritacea/G. margaritaceus in the IUCN Red List and FishBase databases. The data will inform biodiversity and fisheries management at local and regional levels.

Analysis of Prussian carp (Carassius gibelio B.) oocytes under the influence of Roundup® herbicide.

The aim of this study was to investigate the effect of Roundup® herbicide on the maturation of Prussian carp oocytes under laboratory conditions. The Prussian carp is currently one of the most common fish species in Polish freshwater ichthyofauna. For the investigation, oocytes from five sexually mature female Prussian carp were used, segmented into three groups, and incubated for 24 h in Cortland's saline, treated with varied concentrations of the herbicide Roundup® (0 ng - control, R1 - 10 ng/ml, and R2 - 100 ng/ml). Subsequent to this period, assays were performed using the prepared plates to determine the level of 17α,20β-dihydroxyprogesterone (17α,20β-P) utilizing the standard ELISA technique. In determining the 17α,20β-P via ELISA, the medium was extracted from each tested oocyte group. Oocyte maturity was assessed through preservation in serra fluid, and, to categorize the maturity stage of the oocytes utilizing a four-point scale - contingent upon the nucleus's position - the formerly preserved oocytes were dehydrated and subsequently analyzed. A contrast was noted in the percentage of oocytes at varied stages between the control group and the experimental groups. Specifically, a higher concentration of Roundup® (100 ng/ml) accelerated to expedite the initial migration of the nucleus in oocytes. In conclusion, the obtained results show the adverse effect of Roundup® on hormonal regulation and maturation in Prussian carp oocytes.

Entre o passado e o presente: seguindo os passos dos naturalistas do século XIX e sua contribuição para o conhecimento da ictiofauna brasileira

Abstract Aim To evaluate the contribution of naturalists Francis de Castelnau, Jacob Heckel, Johan Baptist von Spix, Johann Natterer, Louis Agassiz, and Rudolf Kner to the knowledge of taxonomic diversity of freshwater ichthyofauna in Brazil between the years 1829 and 1859. Methods Two data matrices were constructed: one with information on freshwater fish species known in Brazil until 2021 and another based on the list of fish species in South America presented by Castelnau (1855). Both were supplemented with information on taxonomy, geographic distribution, authorship, and geographic distribution. The Taxonomic Diversity Index (Δ) was calculated from the first matrix to assess the contribution of the naturalists and determine the number of species per hydrographic region. The second matrix allowed for a detailed analysis of Francis de Castelnau's contribution. Results Between 1829 and 1859, the five naturalists considered in this study described 171 fish species in Brazil, including several endemic species. Castelnau was the naturalist with the greatest contribution (Δ=87.7), followed by Agassiz (85.5), Spix &amp; Agassiz (85.4), Kner (80.8), and Heckel (48.7). The five naturalists described species from four hydrographic regions: the Amazon (122 species), Paraná (25), Tocantins-Araguaia (18), and São Francisco (13). The 276 fish species cataloged by Francis de Castelnau are mostly from the Neotropical region, including some endemics, originating from watercourses in the southeastern Brazilian region, encompassing species collected for the first time in the watercourses of Central Brazil and some marine species, ranging from small (5 cm) to medium (56.0 cm) in size. Conclusions The five naturalists considered in the study made a significant contribution to the early scientific knowledge (1829-1859) of the Brazilian ichthyofauna, but this contribution varies among the naturalists, with particular emphasis on Castelnau, Agassiz, and Spix &amp; Agassiz, as well as among the hydrographic basins, notably the Amazon.

Hematological changes, redox imbalance, and changes in Na+/K+-ATPase activity caused by bisphenol-A and the integrated biomarker responses in Labeo rohita (Hamilton, 1822)

Bisphenol-A (BPA) is a plasticizer commonly used in the plastics industry to manufacture plastic materials. It is abundant in aquatic ecosystems, resulting in increased contamination and lower concentrations that may represent a significant threat to the aquatic system. Hence in the present study, an Indian major carp, Labeo rohita, was exposed to two different BPA concentrations (1 and 10 μg/L) for 30 days. Compared to control, the chronic effects resulted in significant alterations in red blood cell (RBC) and white blood cells (WBC) count. The exposure to BPA caused significant changes in antioxidant activity in gill, liver, and kidney tissues (inferred by catalase, glutathione peroxidase, and glutathione S-transferase activity) in L. rohita. Regarding lipid peroxidation (LPO), we observed an increase in liver and kidney alteration, while LPO was noted in gill tissue compared to the control. Furthermore, increased Na+/K+-ATPase activity was observed in gills at the end of the 10th day and a gradual decrease at the end of the 30th day. These results indicated that exposure to BPA alters the RBC and WBC levels, antioxidant enzyme activity (gills, liver, and kidney), and Na+/K+-ATPase activity in the gill of L. rohita exposed to BPA (at 1 and 10 μg/L). Therefore, our findings will help us gain better insight into the toxicity of BPA in freshwater ichthyofauna.

Haor-Based Floodplain-Rich Freshwater Ichthyofauna in Sylhet Division, Bangladesh: Species Availability, Diversity, and Conservation Perspectives

Biodiversity assessment is important for evaluating community conservation status. The haor basin in Sylhet Division represents a transitional zone with high species availability, rare occurrences and endemism. As a result, this study aims to describe the haor-based freshwater fish composition, including habitat, trophic ecology, availability and conservation status. Semi-structured questionnaires were used to collect data on fish samples through focus group discussions, field surveys, and interviews with fisheries stakeholders on a monthly basis. We identified 188 morpho-species, of which 176 were finfish and 12 shellfish, distributed into 15 orders and 42 families where 29%, 42%, 15%, and 14% species were commonly available, moderately available, abundantly available, and rarely available, respectively. Cypriniformes was the dominant order in both total species and small indigenous species identified. Approximately 45.34% of species were riverine, 31.58% floodplain residents, 12.55% estuarine, 2.83% migratory, and 7.69% were exclusively hill stream residents. Carnivores and omnivores were the most dominant trophic groups. A total of 87.76% species were used as food, 12.23% as ornamental and 6.91% as sport fish. Approximately 50 species were threatened (7 critically endangered, 23 endangered and 20 vulnerable) at the national level, most of them belonging to Cypriniformes and Siluriformes. Based on endemism, 16 species were endemic of which Sygnathidae, Cobitidae, Olyridae, Cyprinidae and Balitoridae fell under the threatened category. Minimizing intense fishing efforts, banning indiscriminate fishing and destructive fishing gear, initiating fish sanctuaries and beel nurseries, and implementing eco-friendly modern fishing technology are suggested to conserve the threatened species. This study represents a guideline for assessing the availability and conservation of freshwater fish in the Sylhet belt and serves as a reference for decision-makers in order to allow for the sustainable exploitation of fisheries resources within an ecosystem-based framework.

“Fishcide” effect of the fungicide difenoconazole in freshwater fish (Labeo rohita): A multi-endpoint approach

Difenoconazole is widely used to protect crops, fruits, and vegetables. However, this fungicide can enter aquatic environments and cause harmful effects to non-target organisms and induce little-known biological disorders. Thus, aiming to expand our knowledge about the ecotoxicity of difenoconazole on freshwater ichthyofauna, we aimed to determine the median lethal concentration (LC50) of difenoconazole and evaluate its possible impacts from different toxicity biomarkers, using freshwater fish Labeo rohita as a model system. Using the probit analysis method, the 96 h LC50 value of difenoconazole in the fish was calculated as 4.5 mg L−1. Posteriorly, fish were exposed to two sublethal concentrations (0.45 mg L−1 1/10th and 0.9 mg L−1 1/5th LC50 value) for 21 days. A significant reduction of superoxide dismutase (SOD) and catalase (CAT) activity was noted in the gill, liver, and kidneys of fish compared to the control groups. The level of glutathione-S-transferase (GST) and lipid peroxidation (LPO) activity was higher in all vital tissues of difenoconazole-treated fish. Histological alterations in the gill include epithelial lifting, lamellar fusion, hypertrophy, and epithelial necrosis. At the same time, the liver showed pyknotic nucleus, vacuolation, cellular edema and tubular necrosis, shrinkage of glomeruli, vacuolation, and pyknotic nuclei in the kidney. DNA damage was increased significantly with tail formation based on the concentration and time-dependent manner. Therefore, our study confirms that the exposure of L. rohita to difenoconazole induces negative biological consequences and sheds light on the danger of this fungicide for freshwater fish species. We believe that studies like ours can support actions and strategies for the remediation/mitigation of aquatic pollution by difenoconazole and for the conservation of freshwater ichthyofauna.

Diversity and distribution of fish in the Qilian Mountain Basin.

The Qilian Mountain Basin, on the north-eastern edge of the Qinghai-Tibet Plateau (QTP), supports a high diversity of native and endemic fish. However, the detailed species inventory and distribution patterns concerning fish in the whole Basin remain unknown, which hinders the conservation of biodiversity and assessment of ecological health. We compiled a comprehensive species richness and distribution database of freshwater fish in the Qilian Mountain Basin, based on field investigations and exhaustive data collection from 50 rivers or lakes. Then, we elucidated a distribution pattern using clustering and ordination analyses based on a βdissim matrix with species presence/absence data. A total of 79 freshwater fish species within eight orders, 17 families and 42 genera were recorded. The Qilian Mountain Basin could be grouped into six systems, which match the six Basins (i.e. Heihe River Basin, HHR; Qaidam Basin, QDM; Qinghai Lake Basin, QHL; Shule River Basin, SLR; Shiyang River Basin, SYR; Yellow River Basin, YR), based on the fish distribution pattern. Additionally, the spatial pattern of species distribution showed the distance decay of taxonomic similarity. Our results demonstrate that riverine connectivity resulting from historical processes plays a vital role in shaping the freshwater ichthyofauna of High Central Asia. These findings will be valuable for future systematic conservation of fish in the Qilian Mountain Basin.

Similarities and Differences in Fish Community Composition Accessed by Electrofishing, Gill Netting, Seining, Trawling, and Water eDNA Metabarcoding in Temperate Reservoirs

It is difficult to understand the composition and diversity of biological communities in complex and heterogeneous environments using traditional sampling methods. Recently, developments in environmental DNA metabarcoding have emerged as a powerful, non-invasive method for comprehensive community characterization and biodiversity monitoring in different types of aquatic ecosystems. In this study, water eDNA targeting fish (wf-eDNA) and four traditional fish sampling methods (electrofishing, gill netting, seining, trawling) were compared to evaluate the reliability and efficiency of wf-eDNA (vertebrate mitochondrial 12S ribosomal RNA (rRNA) as an alternative approach to assess the diversity and composition of freshwater fish communities. The results of wf-eDNA showed a consistency between the traditional sampling methods regarding species detection. However, some fish species detected using wf-eDNA assay were not detected using traditional sampling methods and vice versa. Comparison of wf-eDNA and traditional sampling methods revealed spatial homogeneity in fish community composition in all reservoirs. Ordination analysis showed that the wf-eDNA approach covers all traditional sampling methods and occupies an intermediate position. In addition, based on the Shannon diversity index, we found that in one reservoir the wf-eDNA method yielded similar fish community diversity to traditional sampling methods. However, in other reservoirs, the calculated Shannon diversity index of the wf-eDNA method was significantly higher than traditional sampling methods. In general, significant positive correlations were found between the wf-eDNA method and almost all traditional sampling methods. We conclude that wf-eDNA seems to be a reliable and complementary approach for biomonitoring and ecosystem management of freshwater ichthyofauna.