This study aims to determine the effect of Trichoderma harzianum fermented biourine dosage on the growth of two shallot (Allium ascolonicum L.) varieties. The research employed an experimental method with a Factorial Completely Randomized Design (CRD) consisting of two factors: biourine dosage and shallot variety. The biourine dosage levels were: q0 = Without biourine application, q1 = 5 ml/plant, q2 = 15 ml/plant, q3 = 25 ml/plant, and q4 = 35 ml/plant. The shallot varieties tested were v1 = Keta Monca and v2 = Bali Karet. In total, 10 treatment combinations of biourine dosage and variety were evaluated. The results showed that the most effective biourine dosage for promoting shallot growth was 35 ml (q4). This dosage significantly increased plant height, number of leaves, number of tillers, fresh stover, dry stover, fresh tuber weight, and dry tuber weight. The Keta Monca variety outperformed in terms of number of tillers, fresh tuber weight, and dry tuber weight, while the Bali Karet variety exhibited greater plant height, number of leaves, fresh stover, and dry stover. Significant interactions between biourine dosage and variety were observed in parameters such as plant height at 3 and 4 weeks after planting (WAP), number of leaves at 1, 4, and 5 WAP, number of tillers at 6 and 7 WAP, fresh tuber weight, and dry tuber weight.